##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2138743_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 9181772 Sequences flagged as poor quality 0 Sequence length 19-100 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 23.11056220956042 18.0 18.0 30.0 18.0 33.0 2 30.969428885840337 32.0 30.0 33.0 27.0 33.0 3 31.65194594246078 33.0 31.0 33.0 29.0 33.0 4 32.225621916989446 33.0 33.0 33.0 31.0 34.0 5 32.52731531560575 33.0 33.0 34.0 31.0 34.0 6 36.928722691001255 38.0 37.0 38.0 35.0 38.0 7 37.117761909139105 38.0 38.0 38.0 36.0 38.0 8 37.19844720605129 38.0 38.0 38.0 36.0 38.0 9 37.31722286286351 38.0 38.0 38.0 37.0 38.0 10-11 37.346050903899595 38.0 38.0 38.0 37.0 38.0 12-13 37.37505679731538 38.0 38.0 38.0 37.0 38.0 14-15 37.362495278689124 38.0 38.0 38.0 37.0 38.0 16-17 37.36240548120777 38.0 38.0 38.0 37.0 38.0 18-19 37.24862123563948 38.0 38.0 38.0 37.0 38.0 20-21 37.35105115225693 38.0 38.0 38.0 37.0 38.0 22-23 37.36644535510714 38.0 38.0 38.0 37.0 38.0 24-25 37.37338977294024 38.0 38.0 38.0 37.0 38.0 26-27 37.34340030728987 38.0 38.0 38.0 37.0 38.0 28-29 37.318595778562525 38.0 38.0 38.0 37.0 38.0 30-31 37.32430713519899 38.0 38.0 38.0 37.0 38.0 32-33 37.311140148106986 38.0 38.0 38.0 37.0 38.0 34-35 37.289843112750724 38.0 38.0 38.0 37.0 38.0 36-37 37.26282425983095 38.0 38.0 38.0 37.0 38.0 38-39 37.22588647857885 38.0 38.0 38.0 37.0 38.0 40-41 37.23388515055385 38.0 38.0 38.0 37.0 38.0 42-43 37.209547660451335 38.0 38.0 38.0 37.0 38.0 44-45 37.17503922876076 38.0 38.0 38.0 36.5 38.0 46-47 37.1376812223589 38.0 38.0 38.0 36.0 38.0 48-49 37.100203533010145 38.0 38.0 38.0 36.0 38.0 50-51 37.067947659139485 38.0 38.0 38.0 36.0 38.0 52-53 37.04299907486452 38.0 38.0 38.0 36.0 38.0 54-55 37.025150755211925 38.0 38.0 38.0 36.0 38.0 56-57 37.00723503675371 38.0 38.0 38.0 36.0 38.0 58-59 36.99118927850422 38.0 38.0 38.0 36.0 38.0 60-61 36.984185981170114 38.0 38.0 38.0 36.0 38.0 62-63 36.97585272038597 38.0 38.0 38.0 36.0 38.0 64-65 36.978558029658544 38.0 38.0 38.0 36.0 38.0 66-67 36.96754582888755 38.0 38.0 38.0 36.0 38.0 68-69 36.96753381022522 38.0 38.0 38.0 36.0 38.0 70-71 36.956745211523454 38.0 38.0 38.0 36.0 38.0 72-73 36.92640155401862 38.0 38.0 38.0 36.0 38.0 74-75 36.94566900135848 38.0 38.0 38.0 36.0 38.0 76-77 36.933190525903946 38.0 38.0 38.0 36.0 38.0 78-79 36.921717500057284 38.0 38.0 38.0 36.0 38.0 80-81 36.89672351567625 38.0 38.0 38.0 35.0 38.0 82-83 36.885529786734914 38.0 38.0 38.0 35.0 38.0 84-85 36.868464922462735 38.0 38.0 38.0 35.0 38.0 86-87 36.830582909322715 38.0 38.0 38.0 35.0 38.0 88-89 36.8129157132355 38.0 38.0 38.0 35.0 38.0 90-91 36.8200514402003 38.0 38.0 38.0 35.0 38.0 92-93 36.78823149539146 38.0 38.0 38.0 35.0 38.0 94-95 36.72634942300014 38.0 38.0 38.0 34.5 38.0 96-97 36.74285112113232 38.0 38.0 38.0 35.0 38.0 98-99 36.769502756558204 38.0 38.0 38.0 35.0 38.0 100 35.11486994703049 38.0 35.0 38.0 29.0 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 17 1.0 18 12.0 19 46.0 20 94.0 21 362.0 22 916.0 23 2378.0 24 5286.0 25 10134.0 26 17328.0 27 28382.0 28 42560.0 29 61810.0 30 83376.0 31 108581.0 32 140775.0 33 188014.0 34 276847.0 35 488275.0 36 1470300.0 37 6256295.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.72917515268294 19.42684919642962 16.69921666536699 23.14475898552044 2 14.48429562398195 20.937113228252674 41.953710024600916 22.62488112316446 3 18.560295332970586 25.92045413456139 30.172770571954956 25.34647996051307 4 12.511190650345053 18.258207674945535 38.61386451329874 30.616737161410672 5 13.490912540533959 37.40069023587543 35.63507440342802 13.47332282016259 6 29.864533508521358 37.81594453521625 18.747441937564435 13.572080018697951 7 26.36307022217498 32.499815939668295 23.0046008548241 18.13251298333263 8 24.05916853522392 36.932293679259296 21.213497786701737 17.795039998815042 9 25.296173766893798 16.968630891727653 21.317595339984482 36.41760000139407 10-11 24.2002088485752 26.74256668538491 28.825214784248615 20.232009681791272 12-13 25.01612025522526 24.238470756977563 28.955425431791674 21.789983556005506 14-15 22.104322564315474 25.557702805079458 26.63867606383604 25.69929856676903 16-17 21.75961786025617 28.732531149760632 27.294404609480612 22.21344638050259 18-19 21.74895543038969 27.630554320015786 29.08602500693766 21.534465242656864 20-21 22.45033322474708 26.851376567652775 29.083276072327376 21.61501413527277 22-23 22.17415875021709 26.943546756388216 28.927204244045118 21.955090249349578 24-25 21.967852738456006 27.02307587191834 29.112975201970865 21.89609618765479 26-27 21.912059320350384 27.140496148675417 29.060256538541925 21.887187992432274 28-29 21.712058974874786 27.048391339721668 29.129365844430318 22.11018384097323 30-31 21.993356800641468 26.889104944739707 29.156840678877806 21.960697575741015 32-33 21.757328529814636 27.09782161883861 29.12016672106588 22.024683130280877 34-35 21.942214872094677 27.26252485787172 29.12883213089544 21.666428139138162 36-37 21.950925547388795 27.099740567885288 28.916946455553916 22.032387429172 38-39 21.872445753568154 27.109024630022972 29.123845960234895 21.89468365617398 40-41 22.13345003731848 26.98989325427131 28.996857963936645 21.879798744473568 42-43 22.114301539293038 27.013813939231174 28.871177660513553 22.000706860962236 44-45 22.05594228627039 27.2297440142399 28.74322239112473 21.97109130836498 46-47 22.23755110111555 27.098040204407297 28.370155220479482 22.294253473997674 48-49 22.16422554516807 27.197760249278424 28.393239588207287 22.244774617346224 50-51 22.06930597898689 27.336326144944294 28.351570018095273 22.242797857973542 52-53 22.199531753816537 27.32362579427175 28.418649681127373 22.058192770784338 54-55 22.177729032491232 27.288952129518417 28.266093692573012 22.26722514541734 56-57 22.317080716862744 27.499145504472907 27.999279300096834 22.184494478567515 58-59 22.068322397603854 27.683644457414893 28.327994164246096 21.920038980735164 60-61 22.266925347906177 27.85867477784595 27.98591714439183 21.88848272985604 62-63 22.08390053576596 28.020905735762497 27.963278208947763 21.93191551952378 64-65 22.0180905606184 27.960749171018396 28.00104665646863 22.020113611894573 66-67 22.13383330479252 27.926780778576227 27.834386776522514 22.10499914010874 68-69 22.1283496014328 27.794062748595277 27.966613484869356 22.11097416510257 70-71 22.134977177401378 27.481353468589724 28.050774092505954 22.33289526150294 72-73 22.256796290944855 27.31294960467797 28.091537679131722 22.33871642524545 74-75 22.15281977358771 27.428047555892626 28.058788225868863 22.3603444446508 76-77 22.296848155773574 27.39894187390057 27.900203063299184 22.404006907026677 78-79 22.249916364969675 27.40577152295537 27.893244271146127 22.451067840928825 80-81 22.257154163484756 27.44171992155121 27.859480195367535 22.4416457195965 82-83 22.30700351335876 27.423363558584445 27.982210403966302 22.28742252409049 84-85 22.458312549822153 27.39799319521761 27.8663497583563 22.277344496603934 86-87 22.2949204848377 27.446163086097258 27.870989544274018 22.387926884791025 88-89 22.370329500293177 27.40164261515408 27.847450702543053 22.380577182009688 90-91 22.365622521832552 27.455875721944363 27.864428202379372 22.314073553843716 92-93 22.413423743098075 27.4611937632417 27.851592009625275 22.273790484034954 94-95 22.28132463008122 27.536759460628357 27.860128185401056 22.32178772388937 96-97 22.37586754731113 27.641677474722513 27.89134569750278 22.091109280463574 98-99 23.480588418112816 29.53015133547523 28.102959496747747 18.886300749664205 100 22.41478924241588 30.373920555481583 21.975087118764066 25.236203083338477 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2478.0 1 2415.0 2 2831.0 3 3573.5 4 4534.0 5 6125.5 6 7617.0 7 10058.0 8 12725.0 9 13749.0 10 13473.0 11 13165.0 12 14280.5 13 17325.0 14 23469.5 15 30496.0 16 35315.5 17 37364.5 18 36261.5 19 33260.5 20 30168.5 21 28036.0 22 28672.5 23 32991.0 24 41157.0 25 53370.5 26 69003.5 27 86806.5 28 105894.0 29 126920.0 30 147651.5 31 167658.5 32 187809.0 33 206965.0 34 226405.5 35 245373.0 36 266537.5 37 288027.0 38 303428.5 39 315937.0 40 330747.5 41 344712.0 42 356302.0 43 377907.0 44 393806.5 45 397202.0 46 396632.5 47 390812.5 48 385807.0 49 379839.5 50 371758.5 51 360179.5 52 344762.5 53 326506.0 54 307006.0 55 285066.0 56 261204.5 57 235137.0 58 207551.0 59 180287.5 60 151109.0 61 122022.5 62 95931.5 63 73972.0 64 56643.0 65 42513.0 66 31153.0 67 23247.0 68 17372.0 69 12573.0 70 9187.5 71 6543.5 72 4589.0 73 3112.5 74 2003.0 75 1288.5 76 798.0 77 483.0 78 293.5 79 179.5 80 118.0 81 84.0 82 59.0 83 35.5 84 20.0 85 15.0 86 9.0 87 4.0 88 3.5 89 3.0 90 1.5 91 0.5 92 0.0 93 0.5 94 1.0 95 1.0 96 1.0 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.002973282281459396 6 1.9604058998633382E-4 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 5.4455719440648275E-6 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 5.462679384148452E-5 34-35 1.8067469515662058E-4 36-37 1.757890373341856E-4 38-39 1.4354136986498002E-4 40-41 1.0548205214555212E-4 42-43 1.5633551343206814E-4 44-45 1.3490697068252303E-4 46-47 3.9659917341933414E-5 48-49 2.91222934754014E-4 50-51 4.026652064875807E-4 52-53 4.111367804585183E-4 54-55 0.0019993846791640448 56-57 0.003240578809643822 58-59 0.001904521375147238 60-61 0.0035824005779526787 62-63 0.0020608731456873285 64-65 0.0028709544148767772 66-67 6.478172226486679E-4 68-69 6.789644828741085E-4 70-71 3.5546178570655733E-4 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 7.000534770851145E-6 96-97 0.0 98-99 0.0 100 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 18-19 76.0 20-21 357.0 22-23 831.0 24-25 903.0 26-27 2062.0 28-29 5020.0 30-31 15169.0 32-33 19069.0 34-35 26630.0 36-37 42649.0 38-39 50302.0 40-41 50186.0 42-43 56986.0 44-45 68549.0 46-47 69943.0 48-49 65798.0 50-51 58871.0 52-53 55890.0 54-55 60123.0 56-57 62817.0 58-59 64520.0 60-61 67100.0 62-63 66038.0 64-65 70729.0 66-67 79383.0 68-69 81416.0 70-71 92618.0 72-73 69805.0 74-75 67760.0 76-77 71016.0 78-79 72753.0 80-81 72712.0 82-83 68738.0 84-85 68191.0 86-87 70777.0 88-89 74227.0 90-91 74352.0 92-93 74676.0 94-95 88623.0 96-97 284705.0 98-99 631142.0 100-101 6158260.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 84.6102280933522 #Duplication Level Percentage of deduplicated Percentage of total 1 89.56617948973302 75.78214876076433 2 7.236712744964649 12.245998319950557 3 1.7435738161149308 4.425725348372424 4 0.6679730953150922 2.2606942381932975 5 0.29821342339883805 1.261595288713755 6 0.15729790410540062 0.7985406926978513 7 0.09872174012786207 0.5847008264993736 8 0.05670348513497718 0.383815584876674 9 0.03796855294421169 0.2891275132485843 >10 0.13396140484350322 1.737241909499837 >50 0.0021153762150479733 0.11830933591095959 >100 5.360702234060242E-4 0.07698662646135104 >500 2.1448440080265488E-5 0.01635096424101512 >1k 2.1448438911045138E-5 0.01876459056999195 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 1.0891143888129655E-5 1.0891143888129655E-5 2 0.0 4.356457555251862E-5 0.0 1.0891143888129655E-5 1.0891143888129655E-5 3 0.0 4.356457555251862E-5 0.0 1.0891143888129655E-5 1.0891143888129655E-5 4 0.0 8.712915110503724E-5 0.0 1.0891143888129655E-5 3.2673431664388964E-5 5 0.0 8.712915110503724E-5 0.0 1.0891143888129655E-5 3.2673431664388964E-5 6 4.356457555251862E-5 8.712915110503724E-5 0.0 1.0891143888129655E-5 3.2673431664388964E-5 7 4.356457555251862E-5 9.80202949931669E-5 0.0 1.0891143888129655E-5 3.2673431664388964E-5 8 4.356457555251862E-5 9.80202949931669E-5 0.0 1.0891143888129655E-5 3.2673431664388964E-5 9 4.356457555251862E-5 1.7425830221007448E-4 0.0 3.2673431664388964E-5 4.356457555251862E-5 10-11 4.901014749658345E-5 1.8514944609820412E-4 0.0 4.356457555251862E-5 5.445571944064828E-5 12-13 7.623800721690758E-5 1.8514944609820412E-4 0.0 5.445571944064828E-5 5.9901291384713106E-5 14-15 7.623800721690758E-5 2.559418813710469E-4 0.0 5.9901291384713106E-5 9.257472304910206E-5 16-17 1.4703044248975033E-4 2.9406088497950067E-4 0.0 9.80202949931669E-5 1.0891143888129655E-4 18-19 1.5247601443381517E-4 3.1584317275576E-4 0.0 1.0891143888129655E-4 1.1980258276942621E-4 20-21 1.5247601443381517E-4 3.4851660442014896E-4 0.0 1.0891143888129655E-4 1.4158487054568552E-4 22-23 1.6336715832194484E-4 3.7029889219640825E-4 0.0 1.1980258276942621E-4 1.5792158637788E-4 24-25 1.7425830221007448E-4 4.519824713573807E-4 0.0 1.4703044248975033E-4 1.8514944609820412E-4 26-27 1.7425830221007448E-4 4.901014749658345E-4 0.0 2.6683302525917654E-4 1.960405899863338E-4 28-29 1.7425830221007448E-4 5.06438190798029E-4 0.0 4.302001835811214E-4 1.960405899863338E-4 30-31 1.797038741541393E-4 5.772306260708718E-4 0.0 0.0012906005507433641 2.0148616193039863E-4 32-33 1.8514944609820412E-4 5.772306260708718E-4 0.0 0.002897044274242488 2.2871402165072275E-4 34-35 2.178228777625931E-4 7.024787807843627E-4 0.0 0.005124283199365002 2.2871402165072275E-4 36-37 2.613874533151117E-4 8.277269354978538E-4 0.0 0.008892618984657864 2.2871402165072275E-4 38-39 2.613874533151117E-4 8.604003671622428E-4 0.0 0.017578306235441266 2.341595935947876E-4 40-41 2.722785972032414E-4 9.311928024350856E-4 0.0 0.027402118022534214 2.3960516553885242E-4 42-43 2.722785972032414E-4 9.420839463232151E-4 0.0 0.034018487934572975 2.3960516553885242E-4 44-45 2.722785972032414E-4 0.0010509953852045118 0.0 0.04145169363822147 2.4505073748291726E-4 46-47 2.722785972032414E-4 0.0011435701082536138 0.0 0.050344312622879334 2.504963094269821E-4 48-49 2.777241691473062E-4 0.0011544612521417435 0.0 0.058719602272851036 2.6683302525917654E-4 50-51 2.9406088497950067E-4 0.0012688182629671048 0.0 0.06688796018894828 2.722785972032414E-4 52-53 3.1039760081169517E-4 0.0012851549787992994 0.0 0.08632320645731564 2.777241691473062E-4 54-55 3.1584317275576E-4 0.0013069372665755587 0.0 0.10961391766208091 2.886153130354359E-4 56-57 3.1584317275576E-4 0.0013505018421280772 0.0 0.13824673494397377 3.3762546053201935E-4 58-59 3.1584317275576E-4 0.0013668385579602717 0.0 0.16033397474910072 3.5940774830827863E-4 60-61 3.267343166438897E-4 0.001388620845736531 0.0 0.1694117431798568 3.6485332025234347E-4 62-63 3.4851660442014896E-4 0.0013940664176805959 0.0 0.17964397286275458 4.0297232386079726E-4 64-65 3.4851660442014896E-4 0.0015792158637788 0.0 0.18498063336793813 4.0297232386079726E-4 66-67 3.4851660442014896E-4 0.0016227804393313186 0.0 0.1900177874161981 4.0841789580486204E-4 68-69 3.811900360845379E-4 0.0016500082990516427 0.0 0.19528365548610876 4.356457555251862E-4 70-71 3.8663560802860275E-4 0.001671790586827902 0.0 0.20010843222855024 4.574280433014455E-4 72-73 4.0297232386079726E-4 0.0016772361587719668 0.0 0.20364805399219235 5.173293346861585E-4 74-75 4.0297232386079726E-4 0.0017153551623804207 0.0 0.206305493100896 5.282204785742883E-4 76-77 4.193090396929917E-4 0.0017861475976532633 0.0 0.20891392206210305 5.336660505183531E-4 78-79 4.2475461163705655E-4 0.0018079298854295227 0.0 0.2107164063755885 5.336660505183531E-4 80-81 4.302001835811214E-4 0.0018133754573735877 0.0 0.2112446268541628 5.554483382946124E-4 82-83 4.356457555251862E-4 0.0018242666012617172 0.0 0.2116911637535761 5.772306260708718E-4 84-85 4.356457555251862E-4 0.0018514944609820416 0.0 0.21193076891911497 5.935673419030661E-4 86-87 4.356457555251862E-4 0.0018732767487583007 0.0 0.21205601707382846 6.044584857911959E-4 88 4.356457555251862E-4 0.001982188187639597 0.0 0.21206146264577252 6.099040577352607E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 11725 0.0 49.92753 1 GTATCAA 21230 0.0 35.901165 1 TCAACGC 25750 0.0 29.749626 4 ATCAACG 25805 0.0 29.636213 3 CAACGCA 26300 0.0 29.127485 5 TATCAAC 26900 0.0 28.621708 2 AACGCAG 27160 0.0 28.283289 6 CTTATAC 8430 0.0 27.45045 1 ACGCAGA 30810 0.0 24.932487 7 CGCAGAG 31645 0.0 24.274609 8 TATACAC 11885 0.0 23.921978 3 GCAGAGT 36805 0.0 20.964838 9 TTATACA 12675 0.0 20.564562 2 GTGGTAT 5625 0.0 20.493073 1 TGGTATC 5485 0.0 20.231956 2 GTACATG 26155 0.0 19.783617 1 TACATGG 26265 0.0 19.504246 2 GAGTACT 22830 0.0 19.423552 12-13 ACATGGG 26200 0.0 18.879538 3 CAGAGTA 36165 0.0 18.826447 10-11 >>END_MODULE