FastQCFastQC Report
Fri 27 May 2016
SRR2138742_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138742_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9111556
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA153250.053.465221
GTATCAA289700.037.573921
ATCAACG366300.030.1794053
TATCAAC373550.029.809272
TCAACGC371650.029.7905864
CAACGCA379350.029.18595
TGGTATC59700.028.5424822
AACGCAG392700.028.322316
GTGGTAT66150.026.784681
ACGCAGA442250.025.1586427
CGCAGAG448900.024.7953838
CTTATAC64850.024.5763781
GCAGAGT509250.021.798689
TACATGG345900.021.0529352
GTACATG348450.020.6555731
TATACAC103250.020.6088453
GAGTACT316150.020.27142712-13
ACATGGG351650.020.202423
CAGAGTA500500.019.29191610-11
TATGCCG50300.019.24811742-43
Produced by FastQC (version 0.11.5)