FastQCFastQC Report
Fri 27 May 2016
SRR2138741_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138741_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8393758
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA131750.049.5273171
GTATCAA239050.037.8743131
TCAACGC300800.030.5364954
ATCAACG301650.030.4082533
TATCAAC308950.029.8408132
CAACGCA307500.029.8297565
AACGCAG317600.028.920016
ACGCAGA362200.025.3354767
GTGGTAT59650.024.9652441
CGCAGAG368400.024.8515138
TGGTATC60800.023.3765452
CTTATAC56700.022.7472881
TACATGG308600.021.7220212
GCAGAGT425550.021.5539139
GTACATG310800.021.486361
ACATGGG317800.020.7060383
GAGTACT241400.019.8592212-13
TATACAC84600.019.4079533
CAGAGTA413800.018.92614710-11
AGAGTAC387450.018.66937410-11
Produced by FastQC (version 0.11.5)