##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138741_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8393758
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	23.135862268128292	18.0	18.0	30.0	18.0	33.0
2	30.96678793932348	32.0	30.0	33.0	27.0	33.0
3	31.653188119076106	33.0	31.0	33.0	29.0	33.0
4	32.222967590916966	33.0	33.0	33.0	31.0	34.0
5	32.51258339828239	33.0	33.0	34.0	31.0	34.0
6	36.92628522290016	38.0	37.0	38.0	35.0	38.0
7	37.12062666090683	38.0	38.0	38.0	36.0	38.0
8	37.21975448899051	38.0	38.0	38.0	36.0	38.0
9	37.33333793993108	38.0	38.0	38.0	37.0	38.0
10-11	37.35910941201783	38.0	38.0	38.0	37.0	38.0
12-13	37.38183403667344	38.0	38.0	38.0	37.0	38.0
14-15	37.37492056597296	38.0	38.0	38.0	37.0	38.0
16-17	37.37938269128083	38.0	38.0	38.0	37.0	38.0
18-19	37.26517121413317	38.0	38.0	38.0	37.0	38.0
20-21	37.36964147267206	38.0	38.0	38.0	37.0	38.0
22-23	37.38481780894183	38.0	38.0	38.0	37.0	38.0
24-25	37.39024251823082	38.0	38.0	38.0	37.0	38.0
26-27	37.35947287210944	38.0	38.0	38.0	37.0	38.0
28-29	37.3370767607163	38.0	38.0	38.0	37.0	38.0
30-31	37.34191877111093	38.0	38.0	38.0	37.0	38.0
32-33	37.328436674122216	38.0	38.0	38.0	37.0	38.0
34-35	37.30961132780806	38.0	38.0	38.0	37.0	38.0
36-37	37.282563925441735	38.0	38.0	38.0	37.0	38.0
38-39	37.250684442719916	38.0	38.0	38.0	37.0	38.0
40-41	37.25222315049313	38.0	38.0	38.0	37.0	38.0
42-43	37.22722473881526	38.0	38.0	38.0	37.0	38.0
44-45	37.19190640357726	38.0	38.0	38.0	37.0	38.0
46-47	37.15160706689474	38.0	38.0	38.0	37.0	38.0
48-49	37.117997413029514	38.0	38.0	38.0	36.0	38.0
50-51	37.091317276979936	38.0	38.0	38.0	36.0	38.0
52-53	37.0728341654372	38.0	38.0	38.0	36.0	38.0
54-55	37.05886015693273	38.0	38.0	38.0	36.0	38.0
56-57	37.047344865361325	38.0	38.0	38.0	36.0	38.0
58-59	37.03793847417633	38.0	38.0	38.0	36.0	38.0
60-61	37.035548407129475	38.0	38.0	38.0	36.0	38.0
62-63	37.032059923605246	38.0	38.0	38.0	36.0	38.0
64-65	37.038720561488574	38.0	38.0	38.0	36.0	38.0
66-67	37.03370788154952	38.0	38.0	38.0	36.0	38.0
68-69	37.040244175507965	38.0	38.0	38.0	36.0	38.0
70-71	37.03375267891754	38.0	38.0	38.0	36.0	38.0
72-73	37.00775603520493	38.0	38.0	38.0	36.0	38.0
74-75	37.03227790128152	38.0	38.0	38.0	36.0	38.0
76-77	37.0210738136837	38.0	38.0	38.0	36.0	38.0
78-79	37.0128253874005	38.0	38.0	38.0	36.0	38.0
80-81	36.9913843488431	38.0	38.0	38.0	36.0	38.0
82-83	36.981162803842324	38.0	38.0	38.0	36.0	38.0
84-85	36.965865148655624	38.0	38.0	38.0	36.0	38.0
86-87	36.9297182116772	38.0	38.0	38.0	35.5	38.0
88-89	36.91354068305325	38.0	38.0	38.0	35.0	38.0
90-91	36.919560575526035	38.0	38.0	38.0	35.0	38.0
92-93	36.88535610076487	38.0	38.0	38.0	35.0	38.0
94-95	36.827184019436146	38.0	38.0	38.0	35.0	38.0
96-97	36.84159045616258	38.0	38.0	38.0	35.0	38.0
98-99	36.87028368120964	38.0	38.0	38.0	35.0	38.0
100	35.292393964864615	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	3.0
18	3.0
19	20.0
20	96.0
21	272.0
22	693.0
23	1973.0
24	4264.0
25	8379.0
26	14900.0
27	24684.0
28	36879.0
29	53704.0
30	73256.0
31	95205.0
32	123125.0
33	164063.0
34	243132.0
35	437032.0
36	1352606.0
37	5759469.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.398322420065	19.2542363027383	16.65316059862579	22.694280678570912
2	14.67859807252008	20.89652811053166	41.77654395087397	22.64832986607429
3	18.590445423849484	26.108401028478546	30.12514775860824	25.176005789063733
4	12.677992384340838	18.022416181167006	38.28983394565342	31.00975748883873
5	13.497409002339314	37.56961500497112	35.276586543015206	13.65638944967436
6	30.06874528741006	37.492588225580995	18.582791292414502	13.855875194594446
7	26.589687241400096	32.72739099697656	22.80573254554158	17.87718921608176
8	24.57586935434641	36.559083547559986	20.967390291690563	17.897656806403045
9	25.803650760481776	16.821321272307348	21.062877914755227	36.31215005245564
10-11	24.511851544921832	26.727247795326004	28.386599899592053	20.37430076016011
12-13	25.380175363645225	24.24865000873268	28.679460379963302	21.691714247658798
14-15	22.468145074964642	25.455138860941528	26.550798408865067	25.525917655228763
16-17	21.843606880255543	28.64048498896442	27.237180295166958	22.278727835613083
18-19	21.885054346336887	27.430705054875304	29.20106822236238	21.483172376425433
20-21	22.758712118390967	26.601634057512268	29.15094848324607	21.488705340850693
22-23	22.52043829770749	26.54861117319435	29.040501116008237	21.890449413089925
24-25	22.214230108145703	26.69922489409604	29.15477313116424	21.931771866594012
26-27	22.115580931409824	26.882633069790383	29.08376687372931	21.918019125070483
28-29	22.008565759904698	26.894327688191595	29.127926750469314	21.969179801434397
30-31	22.321286832041032	26.7585653805123	29.016366106357893	21.903781681088773
32-33	21.987603127462762	26.90350936678462	29.026064244651284	22.08282326110134
34-35	22.19715667641346	26.892825165237138	28.957525643701903	21.9524925146475
36-37	22.147181644433207	26.836664008072802	29.00452784548136	22.011626502012625
38-39	21.99890720274396	26.969170519838915	29.04072637007804	21.991195907339087
40-41	22.315536852352466	26.81980615992508	28.90327146166313	21.96138552605932
42-43	22.285808863630624	26.882644033429358	28.647594688129978	22.183952414810033
44-45	22.257980233891537	27.1043609133934	28.49524277110959	22.14241608160547
46-47	22.4087442001061	27.062204465672185	28.167568249056636	22.36148308516508
48-49	22.337827053498653	27.148820755654274	28.105486007408135	22.407866183438934
50-51	22.319075245122978	27.372527227409098	27.929674570827324	22.378722956640598
52-53	22.542063161506633	27.29441316127192	27.817551316288597	22.345972360932855
54-55	22.4544301448001	27.274202521053276	27.721573343384502	22.54979399076212
56-57	22.54901255939277	27.408935873124264	27.599017585450497	22.443033982032464
58-59	22.399857897320793	27.51242244584083	27.736759006594667	22.350960650243714
60-61	22.511963649689623	27.586737000733528	27.649892112788617	22.251407236788232
62-63	22.42838513928108	27.71461477584155	27.519732310868566	22.337267774008804
64-65	22.36349563053628	27.655997306725833	27.554539275628915	22.42596778710897
66-67	22.505020133494376	27.63447072912805	27.42534206439643	22.43516707298114
68-69	22.444680049767136	27.536151123275303	27.563474987926263	22.455693839031294
70-71	22.446418869001317	27.297744178683253	27.643548458249406	22.612288494066025
72-73	22.577584439800166	27.251426835404153	27.573830650011267	22.597158074784414
74-75	22.404059778992863	27.399560220539648	27.60858943902179	22.587790561445697
76-77	22.590714088443708	27.27909041466686	27.475301137802	22.654894359087425
78-79	22.526086394203077	27.296340231327026	27.416375194208953	22.761198180260948
80-81	22.559335174486865	27.381584604845095	27.383171637261995	22.67590858340605
82-83	22.634302799649607	27.33157197215904	27.481777915108545	22.5523473130828
84-85	22.736782879196312	27.333803006145935	27.323508293953996	22.605905820703754
86-87	22.518283731523148	27.4031269709805	27.449703455475305	22.628885842021045
88-89	22.65527979787341	27.353821348702663	27.3161997041094	22.674699149314527
90-91	22.69513342019668	27.366313274940456	27.35049577375414	22.58805753110872
92-93	22.74598305911612	27.368282161172537	27.32399185922951	22.561742920481837
94-95	22.58225944037153	27.50917514626999	27.280929321428932	22.62763609192955
96-97	22.689914173796854	27.521488740038635	27.375108115650832	22.41348897051368
98-99	23.715888379775663	29.502309447531168	27.543827667936338	19.23797450475683
100	22.625744705851417	30.293417645218906	21.539580682726942	25.541256966202734
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2755.0
1	2384.0
2	2298.5
3	3009.0
4	4321.5
5	6179.0
6	7491.5
7	7819.5
8	7569.0
9	7492.5
10	7901.5
11	9307.0
12	11902.0
13	17922.5
14	27578.0
15	37162.5
16	44299.0
17	46886.5
18	45978.0
19	41659.0
20	36484.0
21	33288.0
22	32511.5
23	35751.5
24	42799.5
25	54171.5
26	69686.5
27	86170.0
28	102489.5
29	120392.0
30	137543.5
31	154943.0
32	174311.0
33	192730.0
34	210664.0
35	228470.0
36	245905.0
37	262303.5
38	274404.0
39	282567.5
40	291627.5
41	300529.0
42	307168.0
43	315713.5
44	329561.0
45	344323.5
46	347275.0
47	345204.5
48	345244.5
49	341420.5
50	337175.5
51	332139.5
52	322752.5
53	309686.0
54	296124.5
55	279908.5
56	260304.0
57	237778.5
58	213508.5
59	188719.5
60	161029.0
61	131989.5
62	104166.0
63	80614.5
64	61898.5
65	46482.0
66	34186.5
67	25943.0
68	19897.0
69	14440.0
70	10444.0
71	7561.0
72	5253.0
73	3504.0
74	2299.0
75	1453.0
76	920.0
77	555.5
78	355.0
79	216.0
80	145.5
81	94.5
82	61.0
83	43.0
84	29.5
85	21.5
86	14.0
87	9.0
88	7.5
89	7.0
90	5.5
91	2.5
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.003252416855477606
6	2.263586822493572E-4
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	1.1913614855229326E-5
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	5.386766868510816E-5
34-35	1.1403723315662563E-4
36-37	1.084729224166104E-4
38-39	1.3943360614225646E-4
40-41	6.712839202053348E-5
42-43	1.5359233424346006E-4
44-45	1.362417816015754E-4
46-47	3.750384648825545E-5
48-49	3.027891612834854E-4
50-51	3.4404671440803287E-4
52-53	3.9225722814323436E-4
54-55	0.001983062057788502
56-57	0.002960645956741237
58-59	0.001786801130814348
60-61	0.0033126399765002115
62-63	0.0019256391947956482
64-65	0.002653134956447301
66-67	6.765218358697891E-4
68-69	5.800331861844382E-4
70-71	3.55987070828793E-4
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	1.5308906951698103E-5
90-91	0.0
92-93	0.0
94-95	7.921900831585697E-6
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	1023.0
20-21	3000.0
22-23	1867.0
24-25	2023.0
26-27	3252.0
28-29	6742.0
30-31	17114.0
32-33	21541.0
34-35	29559.0
36-37	45996.0
38-39	55210.0
40-41	53721.0
42-43	61188.0
44-45	73273.0
46-47	74401.0
48-49	68911.0
50-51	85075.0
52-53	58817.0
54-55	63987.0
56-57	66892.0
58-59	66792.0
60-61	65390.0
62-63	63967.0
64-65	68675.0
66-67	74873.0
68-69	76829.0
70-71	82546.0
72-73	68329.0
74-75	67117.0
76-77	70118.0
78-79	72160.0
80-81	71534.0
82-83	66945.0
84-85	65781.0
86-87	69062.0
88-89	72714.0
90-91	74323.0
92-93	72751.0
94-95	80998.0
96-97	253676.0
98-99	557365.0
100-101	5438221.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.35285779568139
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.36006595156805	75.3777693582533
2	7.390457697256359	12.46812454363329
3	1.8252135916556784	4.61885947631029
4	0.6460760239707825	2.179934359008266
5	0.29851343012064036	1.2590230460533725
6	0.1586071912664526	0.8027381910162908
7	0.09345671354958723	0.5518338607670042
8	0.0572232164679702	0.3861553473067347
9	0.03680012992291627	0.279377651362532
>10	0.12893741655613705	1.7257462315566265
>50	0.003987712854612378	0.2261231109928305
>100	6.128115302686643E-4	0.08038164032849027
>500	2.4056640787249093E-5	0.0166601129388163
>1k	2.405663978570515E-5	0.02727307047206136
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.1913614855229326E-5
2	0.0	2.3827229710458653E-5	0.0	0.0	1.1913614855229326E-5
3	0.0	2.3827229710458653E-5	0.0	0.0	1.1913614855229326E-5
4	0.0	8.339530398660528E-5	0.0	1.1913614855229326E-5	1.1913614855229326E-5
5	0.0	8.339530398660528E-5	0.0	1.1913614855229326E-5	1.1913614855229326E-5
6	0.0	8.339530398660528E-5	0.0	1.1913614855229326E-5	1.1913614855229326E-5
7	0.0	8.339530398660528E-5	0.0	1.1913614855229326E-5	1.1913614855229326E-5
8	0.0	8.339530398660528E-5	0.0	1.1913614855229326E-5	1.1913614855229326E-5
9	0.0	1.6679060797321056E-4	0.0	1.1913614855229326E-5	1.1913614855229326E-5
10-11	0.0	1.787042228284399E-4	0.0	5.956807427614663E-5	1.1913614855229326E-5
12-13	0.0	1.787042228284399E-4	0.0	5.956807427614663E-5	2.9784037138073315E-5
14-15	0.0	2.8592675652550385E-4	0.0	5.956807427614663E-5	7.148168913137596E-5
16-17	0.0	2.8592675652550385E-4	0.0	5.956807427614663E-5	7.148168913137596E-5
18-19	0.0	3.157107936635771E-4	0.0	5.956807427614663E-5	8.935211141421995E-5
20-21	0.0	3.693220605121091E-4	0.0	5.956807427614663E-5	1.1913614855229326E-4
22-23	0.0	3.9314929022256774E-4	0.0	8.339530398660528E-5	1.250929559799079E-4
24-25	0.0	4.3484694221587045E-4	0.0	1.0722253369706394E-4	1.3104976340752258E-4
26-27	0.0	4.527173644987144E-4	0.0	1.131793411246786E-4	1.3104976340752258E-4
28-29	0.0	4.646309793539437E-4	0.0	2.740131416702745E-4	1.3700657083513725E-4
30-31	0.0	5.420694759129343E-4	0.0	9.947868404116488E-4	1.4296337826275192E-4
32-33	0.0	5.539830907681637E-4	0.0	0.002198061940789811	1.489201856903666E-4
34-35	0.0	6.37378394754769E-4	0.0	0.004205506043895952	1.5487699311798124E-4
36-37	0.0	7.029032764585303E-4	0.0	0.007160082527992825	1.5487699311798124E-4
38-39	0.0	7.446009284518329E-4	0.0	0.011705126595262813	1.6679060797321056E-4
40-41	0.0	8.696938844317408E-4	0.0	0.01658375187847922	1.787042228284399E-4
42-43	0.0	8.935211141421995E-4	0.0	0.02139685227999187	1.787042228284399E-4
44-45	0.0	9.88830032984034E-4	0.0	0.02662097239400993	1.787042228284399E-4
46-47	0.0	0.0010662685295430247	0.0	0.03236333475423046	1.787042228284399E-4
48-49	0.0	0.0010900957592534833	0.0	0.03857032809380495	1.787042228284399E-4
50-51	0.0	0.0012687999820819233	0.0	0.04447352425457107	1.787042228284399E-4
52-53	0.0	0.0012866704043647673	0.0	0.050489899756461884	1.9061783768366922E-4
54-55	0.0	0.001292627211792382	0.0	0.05609525554584728	1.9061783768366922E-4
56-57	0.0	0.0012985840192199967	0.0	0.061307462045010114	2.0253145253889854E-4
58-59	0.0	0.0013462384786409138	0.0	0.06682346572298128	2.144450673941279E-4
60-61	0.0	0.0013819793232066019	0.0	0.07248838958664283	2.144450673941279E-4
62-63	0.0	0.0013938929380618312	0.0	0.0779626956126207	2.144450673941279E-4
64-65	0.0	0.0014772882420484364	0.0	0.083198729341494	2.144450673941279E-4
66-67	0.0	0.0014892018569036658	0.0	0.0886015536783405	2.144450673941279E-4
68-69	0.0	0.0015428131237521978	0.0	0.09361122872496443	2.263586822493572E-4
70-71	0.0	0.0015845107757455003	0.0	0.09823966809622103	2.3827229710458652E-4
72-73	0.0	0.0015964243906007296	0.0	0.10202224081275635	2.5018591195981587E-4
74-75	0.0	0.0016023811980283443	0.0	0.1047683290368867	2.561427193874305E-4
76-77	0.0	0.0016142948128835737	0.0	0.1077348191358388	2.6209952681504516E-4
78-79	0.0	0.0016202516203111883	0.0	0.10958738624582695	2.6209952681504516E-4
80-81	0.0	0.0016202516203111883	0.0	0.11024859187029218	2.8592675652550385E-4
82-83	0.0	0.0016381220425940324	0.0	0.1107847045387775	3.097539862359625E-4
84-85	0.0	0.001661949272304491	0.0	0.110993192798744	3.1571079366357716E-4
86-87	0.0	0.0016917333094425644	0.0	0.11108850171758586	3.335812159464211E-4
88	0.0	0.001751301383718711	0.0	0.11109445852501347	3.4549483080165047E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	13175	0.0	49.527317	1
GTATCAA	23905	0.0	37.874313	1
TCAACGC	30080	0.0	30.536495	4
ATCAACG	30165	0.0	30.408253	3
TATCAAC	30895	0.0	29.840813	2
CAACGCA	30750	0.0	29.829756	5
AACGCAG	31760	0.0	28.92001	6
ACGCAGA	36220	0.0	25.335476	7
GTGGTAT	5965	0.0	24.965244	1
CGCAGAG	36840	0.0	24.851513	8
TGGTATC	6080	0.0	23.376545	2
CTTATAC	5670	0.0	22.747288	1
TACATGG	30860	0.0	21.722021	2
GCAGAGT	42555	0.0	21.553913	9
GTACATG	31080	0.0	21.48636	1
ACATGGG	31780	0.0	20.706038	3
GAGTACT	24140	0.0	19.85922	12-13
TATACAC	8460	0.0	19.407953	3
CAGAGTA	41380	0.0	18.926147	10-11
AGAGTAC	38745	0.0	18.669374	10-11
>>END_MODULE