FastQCFastQC Report
Fri 27 May 2016
SRR2138740_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138740_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12038148
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA180700.051.029011
GTATCAA339950.036.9798971
TCAACGC430450.029.9332124
ATCAACG431100.029.8684313
CAACGCA436700.029.4757145
TATCAAC441250.029.430952
AACGCAG454300.028.4166476
TGGTATC80000.024.990152
ACGCAGA519300.024.8106337
GTGGTAT83550.024.4352861
CGCAGAG529700.024.3315078
GCAGAGT603950.021.382259
TACATGG438800.020.6471922
GTACATG436500.020.6201341
CTTATAC77150.020.5879061
ACATGGG440750.020.0176833
GAGTACT355000.020.00194412-13
CAGAGTA591100.018.97378510-11
AGAGTAC557050.018.72695710-11
CATGGGG307950.018.6920134
Produced by FastQC (version 0.11.5)