FastQCFastQC Report
Fri 27 May 2016
SRR2138739_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138739_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8062818
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA115650.053.3105931
GTATCAA225900.039.191621
ATCAACG284700.031.4869023
TCAACGC284700.031.4719164
CAACGCA292350.030.648385
TATCAAC293650.030.614412
AACGCAG299000.029.9661776
ACGCAGA342050.026.1323037
CGCAGAG351400.025.4734048
TGGTATC50850.024.5848732
GTGGTAT52700.024.288571
GCAGAGT398600.022.467699
GTACATG285800.021.1841741
TACATGG288100.020.9558122
ACATGGG289900.020.3400123
GAGTACT239550.020.04622512-13
CAGAGTA397000.019.16214210-11
AGAGTAC370600.019.13989310-11
CATGGGG210150.018.069744
GTACTTT266250.018.03623414-15
Produced by FastQC (version 0.11.5)