##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138739_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8062818
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	23.232626607719535	18.0	18.0	30.0	18.0	33.0
2	31.028540517719737	32.0	30.0	33.0	27.0	33.0
3	31.680896679051916	33.0	31.0	33.0	29.0	33.0
4	32.250799534356354	33.0	33.0	33.0	31.0	34.0
5	32.53043365235331	33.0	33.0	34.0	31.0	34.0
6	36.948608538602755	38.0	37.0	38.0	35.0	38.0
7	37.13192856393385	38.0	38.0	38.0	36.0	38.0
8	37.23682513979604	38.0	38.0	38.0	36.0	38.0
9	37.34921115669484	38.0	38.0	38.0	37.0	38.0
10-11	37.37307930056216	38.0	38.0	38.0	37.0	38.0
12-13	37.39878582153287	38.0	38.0	38.0	37.0	38.0
14-15	37.393793708353584	38.0	38.0	38.0	37.0	38.0
16-17	37.39792160755706	38.0	38.0	38.0	37.0	38.0
18-19	37.28364946350023	38.0	38.0	38.0	37.0	38.0
20-21	37.38809130109108	38.0	38.0	38.0	37.0	38.0
22-23	37.40431935874365	38.0	38.0	38.0	37.0	38.0
24-25	37.412967283221825	38.0	38.0	38.0	37.0	38.0
26-27	37.38244437106039	38.0	38.0	38.0	37.0	38.0
28-29	37.35836892955952	38.0	38.0	38.0	37.0	38.0
30-31	37.36372668565255	38.0	38.0	38.0	37.0	38.0
32-33	37.35009360389721	38.0	38.0	38.0	37.0	38.0
34-35	37.32945992664008	38.0	38.0	38.0	37.0	38.0
36-37	37.30185635716707	38.0	38.0	38.0	37.0	38.0
38-39	37.270219176102145	38.0	38.0	38.0	37.0	38.0
40-41	37.265961485097115	38.0	38.0	38.0	37.0	38.0
42-43	37.23833039979621	38.0	38.0	38.0	37.0	38.0
44-45	37.19793565723161	38.0	38.0	38.0	37.0	38.0
46-47	37.15403786314943	38.0	38.0	38.0	37.0	38.0
48-49	37.111593769945515	38.0	38.0	38.0	36.5	38.0
50-51	37.078610818713855	38.0	38.0	38.0	36.0	38.0
52-53	37.06058511875523	38.0	38.0	38.0	36.0	38.0
54-55	37.04999492814412	38.0	38.0	38.0	36.0	38.0
56-57	37.04109103766079	38.0	38.0	38.0	36.0	38.0
58-59	37.034448997639196	38.0	38.0	38.0	36.0	38.0
60-61	37.03698957300928	38.0	38.0	38.0	36.0	38.0
62-63	37.03613378701062	38.0	38.0	38.0	36.0	38.0
64-65	37.04533519101441	38.0	38.0	38.0	36.0	38.0
66-67	37.04265657006154	38.0	38.0	38.0	36.0	38.0
68-69	37.0479147817532	38.0	38.0	38.0	36.0	38.0
70-71	37.04431091592359	38.0	38.0	38.0	36.0	38.0
72-73	37.02285116531146	38.0	38.0	38.0	36.0	38.0
74-75	37.044087968754184	38.0	38.0	38.0	36.0	38.0
76-77	37.03814036263776	38.0	38.0	38.0	36.0	38.0
78-79	37.031906622761454	38.0	38.0	38.0	36.0	38.0
80-81	37.01145812888059	38.0	38.0	38.0	36.0	38.0
82-83	37.005988513521146	38.0	38.0	38.0	36.0	38.0
84-85	36.99416204912322	38.0	38.0	38.0	36.0	38.0
86-87	36.96135440718945	38.0	38.0	38.0	36.0	38.0
88-89	36.948888884852565	38.0	38.0	38.0	36.0	38.0
90-91	36.95593218015415	38.0	38.0	38.0	36.0	38.0
92-93	36.9277244512689	38.0	38.0	38.0	35.0	38.0
94-95	36.87144589995974	38.0	38.0	38.0	35.0	38.0
96-97	36.8890476493917	38.0	38.0	38.0	35.0	38.0
98-99	36.92071631742346	38.0	38.0	38.0	35.0	38.0
100	35.39230286106197	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	5.0
19	22.0
20	66.0
21	250.0
22	622.0
23	1598.0
24	3775.0
25	7618.0
26	13485.0
27	22501.0
28	34554.0
29	50938.0
30	70189.0
31	91471.0
32	116923.0
33	155067.0
34	227082.0
35	404639.0
36	1262725.0
37	5599288.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.47670206620068	19.362510725158373	16.509066681152916	22.65172052748803
2	14.714073913115739	20.95847878496079	41.78506819824037	22.542379103683103
3	18.605939015366587	26.138330295933756	30.14116156410823	25.11456912459143
4	12.688045296321956	17.911616013160657	38.410714467323956	30.98962422319343
5	13.505914174608396	37.51658123287846	35.27258461591508	13.70491997659806
6	30.084972899013074	37.51060114803705	18.614226858490703	13.790199094459174
7	26.48350737918182	32.73808239253323	23.015228670670727	17.763181557614224
8	24.452815380429026	36.61654027165192	21.023083988749345	17.90756035916971
9	25.71086188476535	16.839335825266055	21.340491624640418	36.109310665328174
10-11	24.450706936458197	26.733909905941072	28.512053726129004	20.303329431471724
12-13	25.292515594423687	24.332720892372866	28.87384410760605	21.5009194055974
14-15	22.376816641526574	25.48297009804761	26.772947125930415	25.36726613449541
16-17	21.696260538189005	28.642299751774132	27.422843973409794	22.23859573662707
18-19	21.751228912769704	27.46989948179408	29.446175022182068	21.332696583254144
20-21	22.60066281589907	26.63306676287901	29.467705166301478	21.298565254920437
22-23	22.38041971728982	26.527749861687443	29.334969166269087	21.75686125475365
24-25	22.07875154982996	26.70390744763728	29.41205912372982	21.80528187880294
26-27	21.962157087567675	26.848128711431123	29.391113623950904	21.798600577050294
28-29	21.871492865868298	26.862217449126057	29.460959824604434	21.80532986040121
30-31	22.213185708371388	26.712729907854886	29.317686687233895	21.75639769653983
32-33	21.924172684675465	26.902709969240124	29.265615517050726	21.907501829033684
34-35	22.095356870799314	26.899529155018236	29.26885204786685	21.7362619263156
36-37	22.048354184847	26.856398051373098	29.225270123162613	21.869977640617293
38-39	21.910896782275337	26.917034208989925	29.342101865538396	21.829967143196335
40-41	22.16640914829959	26.78673923950615	29.188742046066608	21.858109566127656
42-43	22.07687289370232	26.87908970375077	28.98195455240921	22.0620828501377
44-45	22.123422512486936	27.01520025304836	28.833753988101535	22.027623246363166
46-47	22.224244553791557	26.995249653374024	28.570506897083646	22.20999889575077
48-49	22.165952537418594	27.123731192570222	28.492949043588002	22.217367226423185
50-51	22.165078921543113	27.29376052018434	28.338672872128317	22.202487686144227
52-53	22.36422859275235	27.198896534414267	28.249531265755618	22.187343607077768
54-55	22.312718234188388	27.147283080014383	28.226168536927254	22.31383014886997
56-57	22.286089507947683	27.24925167344311	28.217486749361836	22.247172069247377
58-59	22.25307767091683	27.28897165451989	28.272056496807966	22.185894177755316
60-61	22.347198859061542	27.266801908243487	28.258086337414518	22.127912895280456
62-63	22.3314148088064	27.370598006368223	28.18124900548501	22.116738179340366
64-65	22.265536485444308	27.289264895275302	28.207535973538583	22.23766264574181
66-67	22.341317413844976	27.316862926842518	28.0578866117371	22.283933047575413
68-69	22.2811314717105	27.26146028134917	28.176255404341482	22.281152842598846
70-71	22.277990394333713	27.08640739071874	28.220584355972356	22.415017858975187
72-73	22.419743031442152	27.08058296064201	28.08751286394786	22.412161143967975
74-75	22.27163780354341	27.218614357292726	28.0768292401055	22.432918599058365
76-77	22.463941802762047	27.113048108048154	27.964921559107346	22.458088530082453
78-79	22.36625513171194	27.156756135299954	27.93137068804461	22.545618044943495
80-81	22.415114501380305	27.25029457993344	27.88546329808963	22.449127620596627
82-83	22.45206411030495	27.23293314510836	27.930019191091628	22.384983553495065
84-85	22.516124585602377	27.22432529201463	27.802168447747714	22.45738167463528
86-87	22.410799473113535	27.278099123848953	27.856389527410407	22.454711875627105
88-89	22.45817561613391	27.23016052002505	27.785594986091688	22.526068877749356
90-91	22.49130175446141	27.316966536143212	27.769187704515286	22.422544004880095
92-93	22.581514509898398	27.25715703774036	27.765958440450834	22.395370011910416
94-95	22.438794883372132	27.33179994774696	27.768246376330026	22.46115879255088
96-97	22.56400000893592	27.358964916006904	27.77683500146822	22.300200073588954
98-99	23.603044340738474	29.323107584536018	27.993309129446697	19.080538945278814
100	22.47653548493331	30.179641577927267	21.956146698350498	25.387676238788927
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2234.0
1	2164.5
2	2894.5
3	4006.0
4	4939.0
5	6622.0
6	8339.0
7	11432.0
8	14998.5
9	16452.0
10	16020.5
11	15104.0
12	15892.0
13	19012.5
14	25318.0
15	32373.0
16	36969.0
17	38683.0
18	37448.0
19	34231.0
20	30742.0
21	28327.0
22	28544.5
23	32602.0
24	40354.5
25	51396.0
26	65804.0
27	81986.0
28	99053.5
29	117952.5
30	135509.0
31	152353.5
32	170640.0
33	188166.5
34	204149.5
35	219058.0
36	236159.0
37	252751.0
38	264882.5
39	273167.5
40	281345.0
41	290140.5
42	296324.0
43	303761.5
44	309667.5
45	313586.5
46	316815.5
47	318446.5
48	320919.0
49	319729.0
50	315017.0
51	310203.0
52	302722.0
53	291904.0
54	279863.0
55	265065.0
56	247089.0
57	226148.5
58	202863.5
59	178642.5
60	152450.5
61	125527.0
62	100152.0
63	77889.0
64	59482.5
65	44928.0
66	33694.0
67	25390.0
68	19115.0
69	14065.5
70	10385.5
71	7599.5
72	5387.5
73	3629.5
74	2346.0
75	1525.0
76	956.5
77	565.0
78	350.5
79	220.0
80	139.5
81	82.5
82	56.0
83	47.0
84	33.5
85	20.5
86	12.5
87	11.0
88	9.5
89	5.5
90	3.0
91	2.5
92	1.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.002964224170755188
6	1.4883133911741526E-4
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	3.733672881842259E-5
34-35	1.3101539237456728E-4
36-37	1.4404376826263613E-4
38-39	1.4485439708356455E-4
40-41	9.50602149425532E-5
42-43	1.530661932839529E-4
44-45	1.5423484543033642E-4
46-47	3.8904831584550344E-5
48-49	2.9439549483303195E-4
50-51	4.3536725701807076E-4
52-53	4.5864522316347146E-4
54-55	0.001996047959004122
56-57	0.003403978034616025
58-59	0.002030042033450869
60-61	0.0034632665268762193
62-63	0.002238474920799223
64-65	0.0026349450040848636
66-67	6.278028930285945E-4
68-69	7.266049242443131E-4
70-71	4.0295683972463656E-4
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	7.877161650700185E-6
90-91	0.0
92-93	0.0
94-95	8.14417602235674E-6
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	57.0
20-21	262.0
22-23	597.0
24-25	749.0
26-27	2008.0
28-29	4982.0
30-31	14920.0
32-33	18953.0
34-35	26940.0
36-37	41925.0
38-39	49578.0
40-41	49116.0
42-43	56113.0
44-45	67715.0
46-47	69546.0
48-49	64667.0
50-51	58641.0
52-53	56400.0
54-55	60804.0
56-57	63176.0
58-59	63475.0
60-61	62499.0
62-63	60332.0
64-65	64046.0
66-67	68802.0
68-69	69689.0
70-71	72288.0
72-73	64457.0
74-75	64921.0
76-77	67856.0
78-79	69208.0
80-81	68912.0
82-83	64922.0
84-85	64260.0
86-87	65665.0
88-89	68328.0
90-91	69265.0
92-93	68932.0
94-95	79849.0
96-97	245634.0
98-99	536673.0
100-101	5295656.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.83137408791156
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.45578089694666	77.63943968579993
2	6.708996034321056	11.516846967522513
3	1.5855115937042332	4.082599161598467
4	0.5658283490724093	1.942632987951175
5	0.2592135089400503	1.1124325827236832
6	0.1312928448453781	0.6761427168593866
7	0.08683891104770575	0.5217452141665756
8	0.051789072861875174	0.3556101829178973
9	0.033412531416134594	0.25810591348821116
>10	0.11765130892593743	1.5901466606083314
>50	0.002897535238239049	0.16740743551363754
>100	7.164844793589812E-4	0.08644257946258388
>500	4.7285702911985486E-5	0.025670547924258734
>1k	2.3642498078033616E-5	0.02477736346325197
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	2.4805223186235878E-5	0.0
3	0.0	0.0	0.0	3.720783477935382E-5	0.0
4	0.0	3.720783477935382E-5	0.0	4.9610446372471755E-5	0.0
5	1.2402611593117939E-5	3.720783477935382E-5	0.0	7.441566955870764E-5	0.0
6	2.4805223186235878E-5	3.720783477935382E-5	0.0	7.441566955870764E-5	1.2402611593117939E-5
7	2.4805223186235878E-5	4.9610446372471755E-5	0.0	8.681828115182558E-5	1.2402611593117939E-5
8	2.4805223186235878E-5	6.20130579655897E-5	0.0	9.922089274494351E-5	1.2402611593117939E-5
9	2.4805223186235878E-5	7.441566955870764E-5	0.0	1.240261159311794E-4	2.4805223186235878E-5
10-11	4.9610446372471755E-5	7.441566955870764E-5	0.0	1.7363656230365117E-4	2.4805223186235878E-5
12-13	7.441566955870764E-5	7.441566955870764E-5	0.0	1.7363656230365117E-4	2.4805223186235878E-5
14-15	7.441566955870764E-5	9.301958694838455E-5	0.0	2.1084439708300495E-4	4.340914057591279E-5
16-17	8.681828115182558E-5	9.922089274494351E-5	0.0	2.4185092606579983E-4	6.20130579655897E-5
18-19	8.681828115182558E-5	1.1782481013462043E-4	0.0	2.542535376589178E-4	6.20130579655897E-5
20-21	8.681828115182558E-5	1.3642872752429733E-4	0.0	2.914613724382716E-4	8.06169753552666E-5
22-23	9.922089274494352E-5	1.612339507105332E-4	0.0	3.1006528982794847E-4	9.922089274494351E-5
24-25	1.1782481013462043E-4	1.7363656230365117E-4	0.0	3.348705130141844E-4	1.1162350433806145E-4
26-27	1.240261159311794E-4	1.7363656230365117E-4	0.0	3.720783477935382E-4	1.1162350433806145E-4
28-29	1.240261159311794E-4	1.7363656230365117E-4	0.0	5.829227448765431E-4	1.1162350433806145E-4
30-31	1.240261159311794E-4	1.9844178548988702E-4	0.0	0.0011782481013462042	1.2402611593117938E-4
32-33	1.3642872752429733E-4	2.04643091286446E-4	0.0	0.002740977162079065	1.4883133911741529E-4
34-35	1.3642872752429733E-4	2.7285745504859466E-4	0.0	0.004954843331450617	1.4883133911741529E-4
36-37	1.3642872752429733E-4	3.348705130141844E-4	0.0	0.008173321039864722	1.5503264491397426E-4
38-39	1.3642872752429733E-4	3.534744304038613E-4	0.0	0.013140566982908457	1.612339507105332E-4
40-41	1.3642872752429733E-4	4.09286182572892E-4	0.0	0.01798378681002101	1.612339507105332E-4
42-43	1.3642872752429733E-4	4.15487488369451E-4	0.0	0.023149474538554635	1.674352565070922E-4
44-45	1.3642872752429733E-4	4.5889662894536376E-4	0.0	0.02902211112789598	1.7363656230365117E-4
46-47	1.3642872752429733E-4	4.961044637247176E-4	0.0	0.03549627437950355	1.7983786810021012E-4
48-49	1.3642872752429733E-4	4.961044637247176E-4	0.0	0.04188982065575584	1.860391738967691E-4
50-51	1.3642872752429733E-4	5.457149100971893E-4	0.0	0.048506613940684264	1.860391738967691E-4
52-53	1.3642872752429733E-4	5.457149100971893E-4	0.0	0.05476993279520882	2.1084439708300498E-4
54-55	1.426300333208563E-4	5.457149100971893E-4	0.0	0.06110146601349553	2.480522318623588E-4
56-57	1.4883133911741529E-4	5.457149100971893E-4	0.0	0.06663303078402613	2.480522318623588E-4
58-59	1.4883133911741529E-4	5.519162158937484E-4	0.0	0.0719413485458806	2.480522318623588E-4
60-61	1.5503264491397426E-4	5.829227448765432E-4	0.0	0.07739229634105595	2.480522318623588E-4
62-63	1.7363656230365117E-4	6.13929273859338E-4	0.0	0.08240295142467559	2.604548434554767E-4
64-65	1.7363656230365117E-4	6.945462492146047E-4	0.0	0.0872833790865675	2.604548434554767E-4
66-67	1.7363656230365117E-4	7.131501666042814E-4	0.0	0.09217000805425596	2.666561492520357E-4
68-69	1.7363656230365117E-4	7.503580013836354E-4	0.0	0.097273682724824	2.7285745504859466E-4
70-71	1.9224047969332807E-4	7.689619187733123E-4	0.0	0.10193086337803979	2.7285745504859466E-4
72-73	1.9844178548988702E-4	7.751632245698713E-4	0.0	0.1059059003936341	2.9766267823483057E-4
74-75	1.9844178548988702E-4	7.813645303664302E-4	0.0	0.10891353370496519	2.9766267823483057E-4
76-77	1.9844178548988702E-4	8.247736709423429E-4	0.0	0.11187155656992381	2.9766267823483057E-4
78-79	2.1084439708300498E-4	8.433775883320199E-4	0.0	0.11381256528424677	2.9766267823483057E-4
80-81	2.1084439708300498E-4	8.433775883320199E-4	0.0	0.1145939298146132	3.100652898279485E-4
82-83	2.1084439708300498E-4	8.495788941285788E-4	0.0	0.11510243688993105	3.224679014210664E-4
84-85	2.232470086761229E-4	8.557801999251379E-4	0.0	0.1153814956507762	3.224679014210664E-4
86-87	2.3564962026924086E-4	8.557801999251379E-4	0.0	0.11549311915511426	3.224679014210664E-4
88	2.3564962026924086E-4	9.301958694838455E-4	0.0	0.11549311915511426	3.348705130141844E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11565	0.0	53.310593	1
GTATCAA	22590	0.0	39.19162	1
ATCAACG	28470	0.0	31.486902	3
TCAACGC	28470	0.0	31.471916	4
CAACGCA	29235	0.0	30.64838	5
TATCAAC	29365	0.0	30.61441	2
AACGCAG	29900	0.0	29.966177	6
ACGCAGA	34205	0.0	26.132303	7
CGCAGAG	35140	0.0	25.473404	8
TGGTATC	5085	0.0	24.584873	2
GTGGTAT	5270	0.0	24.28857	1
GCAGAGT	39860	0.0	22.46769	9
GTACATG	28580	0.0	21.184174	1
TACATGG	28810	0.0	20.955812	2
ACATGGG	28990	0.0	20.340012	3
GAGTACT	23955	0.0	20.046225	12-13
CAGAGTA	39700	0.0	19.162142	10-11
AGAGTAC	37060	0.0	19.139893	10-11
CATGGGG	21015	0.0	18.06974	4
GTACTTT	26625	0.0	18.036234	14-15
>>END_MODULE