FastQCFastQC Report
Fri 27 May 2016
SRR2138738_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138738_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8925455
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA111800.048.2210581
GTATCAA201650.035.7739871
CTTATAC75150.034.787261
ATCAACG245150.029.303993
TCAACGC247450.029.083494
CAACGCA252700.028.4115355
TATCAAC256650.028.2243332
AACGCAG260150.027.6463176
TATACAC113550.026.9418163
TTATACA114700.024.5081162
ACGCAGA298050.024.0733937
CGCAGAG307150.023.4158828
TACACAT154600.020.2032555
GCAGAGT356800.020.1814639
GTACATG251450.020.1298751
TACATGG255350.019.8056722
TGGTATC56900.019.7765922
ACACATC142350.019.6267816
GTGGTAT58000.019.622831
ACATCTC146000.019.4584668
Produced by FastQC (version 0.11.5)