##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138738_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8925455
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	23.69775961001428	18.0	18.0	31.0	18.0	33.0
2	31.16557049472548	32.0	30.0	33.0	27.0	33.0
3	31.749482575398115	33.0	31.0	33.0	29.0	33.0
4	32.27948916889951	33.0	33.0	33.0	31.0	34.0
5	32.542645500985664	33.0	33.0	34.0	31.0	34.0
6	36.94530474916965	38.0	37.0	38.0	35.0	38.0
7	37.126908600177806	38.0	38.0	38.0	36.0	38.0
8	37.23057726468846	38.0	38.0	38.0	36.0	38.0
9	37.342044299142174	38.0	38.0	38.0	37.0	38.0
10-11	37.36954149676403	38.0	38.0	38.0	37.0	38.0
12-13	37.39361276259866	38.0	38.0	38.0	37.0	38.0
14-15	37.38549973082605	38.0	38.0	38.0	37.0	38.0
16-17	37.38991468782264	38.0	38.0	38.0	37.0	38.0
18-19	37.27067258755996	38.0	38.0	38.0	37.0	38.0
20-21	37.37772855448874	38.0	38.0	38.0	37.0	38.0
22-23	37.395014813972296	38.0	38.0	38.0	37.0	38.0
24-25	37.40291916116957	38.0	38.0	38.0	37.0	38.0
26-27	37.37346912836469	38.0	38.0	38.0	37.0	38.0
28-29	37.3463231811647	38.0	38.0	38.0	37.0	38.0
30-31	37.351186388847424	38.0	38.0	38.0	37.0	38.0
32-33	37.33569515559816	38.0	38.0	38.0	37.0	38.0
34-35	37.313624317609076	38.0	38.0	38.0	37.0	38.0
36-37	37.286456532790865	38.0	38.0	38.0	37.0	38.0
38-39	37.243266550165586	38.0	38.0	38.0	37.0	38.0
40-41	37.26746447658529	38.0	38.0	38.0	37.0	38.0
42-43	37.24957001169217	38.0	38.0	38.0	37.0	38.0
44-45	37.21822851814332	38.0	38.0	38.0	37.0	38.0
46-47	37.184416849436374	38.0	38.0	38.0	37.0	38.0
48-49	37.15337226987465	38.0	38.0	38.0	37.0	38.0
50-51	37.12449499619203	38.0	38.0	38.0	36.0	38.0
52-53	37.102647998535645	38.0	38.0	38.0	36.0	38.0
54-55	37.08961002020794	38.0	38.0	38.0	36.0	38.0
56-57	37.07808923981602	38.0	38.0	38.0	36.0	38.0
58-59	37.06679413394161	38.0	38.0	38.0	36.0	38.0
60-61	37.06339871079432	38.0	38.0	38.0	36.0	38.0
62-63	37.05684240793309	38.0	38.0	38.0	36.0	38.0
64-65	37.06165970539689	38.0	38.0	38.0	36.0	38.0
66-67	37.05068140440741	38.0	38.0	38.0	36.0	38.0
68-69	37.05111102554382	38.0	38.0	38.0	36.0	38.0
70-71	37.04238521003328	38.0	38.0	38.0	36.0	38.0
72-73	37.0171635936115	38.0	38.0	38.0	36.0	38.0
74-75	37.037202048284385	38.0	38.0	38.0	36.0	38.0
76-77	37.02850122499221	38.0	38.0	38.0	36.0	38.0
78-79	37.019730888261904	38.0	38.0	38.0	36.0	38.0
80-81	36.99858260171827	38.0	38.0	38.0	36.0	38.0
82-83	36.988492741254696	38.0	38.0	38.0	36.0	38.0
84-85	36.97582857434982	38.0	38.0	38.0	36.0	38.0
86-87	36.945009908665995	38.0	38.0	38.0	36.0	38.0
88-89	36.9290632211037	38.0	38.0	38.0	36.0	38.0
90-91	36.934845795514256	38.0	38.0	38.0	36.0	38.0
92-93	36.90503609678973	38.0	38.0	38.0	35.0	38.0
94-95	36.846014781759116	38.0	38.0	38.0	35.0	38.0
96-97	36.864764562067094	38.0	38.0	38.0	35.0	38.0
98-99	36.89167183863779	38.0	38.0	38.0	35.0	38.0
100	35.32407374502177	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	7.0
19	32.0
20	83.0
21	232.0
22	767.0
23	1908.0
24	4409.0
25	8520.0
26	15205.0
27	24846.0
28	38674.0
29	55102.0
30	75074.0
31	98169.0
32	127702.0
33	170627.0
34	251675.0
35	448554.0
36	1380784.0
37	6223085.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.68299039096606	19.776795692768605	16.1977736709221	23.342440245343234
2	14.3864374421248	20.85881336021525	42.18275706952755	22.571992128132404
3	18.346190754420924	26.10300539300237	30.345713467828812	25.20509038474789
4	12.495687894902836	18.26450304214183	38.524691458306606	30.71511760464873
5	13.366461433934123	37.45165348444486	35.76923956028984	13.412645521331177
6	29.813335854963825	37.83569485970555	18.698054393272624	13.652914892058007
7	26.23534598516266	32.50604030830922	23.161575516318216	18.09703819020991
8	23.947193728498995	37.117883626100856	21.255006047310754	17.679916598089395
9	25.16454343223959	17.029988947342183	21.460250485829572	36.34521713458866
10-11	24.140836517578094	26.795311835643115	28.88147999177633	20.182371655002463
12-13	24.93444451484235	24.22964007043003	29.01038821048273	21.825527204244892
14-15	22.082871078602835	25.595728799901945	26.560727331069096	25.76067279042612
16-17	21.749182534671903	28.795103442905713	27.287348376077187	22.1683656463452
18-19	21.76393808494917	27.69606703523798	29.020974280862994	21.519020598949858
20-21	22.431871700233522	26.91802195936909	28.98508433425141	21.66502200614598
22-23	22.214712101174758	27.00775227498778	28.84054923068287	21.936986393154594
24-25	22.06121873737832	27.160544735034964	28.95014334616467	21.82809318142205
26-27	21.972932607195578	27.320013212352713	28.905540363093156	21.801513817358558
28-29	21.709880113280434	27.14098060132295	29.06103560832241	22.08810367707421
30-31	22.14642587694819	26.983870379667312	29.11958805101122	21.750115692373278
32-33	21.82893212641103	27.291124319808112	28.953519644967844	21.92642390881301
34-35	21.927333848927812	27.273475931069562	29.169795251160792	21.629394968841833
36-37	22.129387904016397	27.11477564251497	28.87037252476521	21.885463928703423
38-39	21.85258370157923	27.13479808811999	29.0700461012288	21.94257210907197
40-41	22.101932475613705	26.973452867184545	29.00593378523964	21.918680871962117
42-43	22.12901691541007	27.033026540091647	28.858629554635385	21.979326989862898
44-45	21.99370812072811	27.292384197226617	28.715254535642405	21.99865314640287
46-47	22.242653541216278	27.076694533182344	28.38724476939421	22.293407156207167
48-49	22.142503279959236	27.14563019012932	28.372954204893613	22.338912325017823
50-51	22.000173208551992	27.321549219997994	28.418843192281066	22.259434379168944
52-53	22.145678746818653	27.320512360946815	28.473647106618394	22.060161785616135
54-55	22.14504974331998	27.301290161412656	28.2541323277688	22.299527767498567
56-57	22.276597783811194	27.550339804294076	28.00249424358666	22.170568168308062
58-59	21.97329109647158	27.7884752686561	28.328878791724062	21.909354843148257
60-61	22.25165454297027	28.00217481701004	27.886201429785572	21.85996921023412
62-63	22.00587805370337	28.220434351183677	27.883135845768308	21.89055174934465
64-65	21.990510685670383	28.11057904887342	27.87899713062703	22.01991313482917
66-67	22.085244532122324	28.03454418985436	27.78556906862179	22.094642209401528
68-69	22.065160604229174	27.894078493157508	27.933892063235216	22.106868839378105
70-71	22.06704438528127	27.59244447131195	28.08644128768486	22.254069855721916
72-73	22.26842999383935	27.369707273573912	28.03217346665769	22.329689265929048
74-75	22.14064679979649	27.493143063255175	28.010196553179473	22.356013583768867
76-77	22.26894089911655	27.46978127478329	27.912369783977276	22.34890804212288
78-79	22.20022501523337	27.445443711051976	27.91207708655068	22.44225418716397
80-81	22.22458144448101	27.51293919984799	27.910996706979425	22.35148264869157
82-83	22.324018036930884	27.48573592875892	27.93323660726405	22.257009427046146
84-85	22.428182060890112	27.446501491142232	27.826613711181874	22.298702736785785
86-87	22.255979646724853	27.4875553842232	27.87925981900307	22.377205150048873
88-89	22.33423852951323	27.450548867325363	27.863509389567543	22.351703213593865
90-91	22.369184148564056	27.548006299246836	27.816179098611588	22.266630453577523
92-93	22.433743289596393	27.485711993831323	27.828270035638266	22.25227468093402
94-95	22.250926626034985	27.592498031529285	27.8217005731196	22.33487476931613
96-97	22.381506383218063	27.67494238070797	27.83894245698708	22.10460877908688
98-99	23.45958089171413	29.57866765834175	28.054560296156072	18.907191153788048
100	22.40894154327487	30.474143314200543	21.940572250917466	25.176342891607124
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1618.0
1	1600.0
2	2178.5
3	3048.0
4	3971.0
5	5483.5
6	6862.5
7	8808.5
8	11001.5
9	11973.0
10	11885.5
11	11769.5
12	12890.5
13	15744.0
14	21372.0
15	27964.5
16	32523.0
17	34661.5
18	34238.5
19	31591.0
20	28425.0
21	26644.5
22	27438.5
23	31754.5
24	39797.0
25	51563.0
26	66913.0
27	84679.5
28	103215.0
29	124357.0
30	146287.0
31	166776.5
32	188066.0
33	208969.5
34	230501.0
35	251205.5
36	272347.0
37	291602.5
38	305854.0
39	318608.0
40	331973.0
41	342189.0
42	350227.0
43	370830.5
44	385597.5
45	388503.0
46	385408.0
47	375433.5
48	368813.0
49	362805.5
50	355036.0
51	343937.0
52	330716.0
53	314019.5
54	294413.0
55	273682.5
56	251853.5
57	228774.5
58	203990.0
59	177222.0
60	148708.0
61	120699.0
62	95403.5
63	73719.5
64	55767.0
65	41650.5
66	30847.5
67	23172.0
68	17575.0
69	12942.0
70	9444.0
71	6900.0
72	4876.5
73	3171.5
74	2076.0
75	1369.5
76	833.5
77	502.5
78	322.5
79	211.5
80	135.5
81	84.5
82	51.0
83	30.0
84	19.0
85	14.5
86	11.5
87	7.5
88	3.0
89	1.0
90	0.5
91	0.5
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0033947849157269856
6	1.4565083796848452E-4
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	5.601955306480174E-6
14-15	1.1203910612960348E-5
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	6.181387650722601E-5
34-35	1.126570791737054E-4
36-37	9.609973569746221E-5
38-39	1.704861144458511E-4
40-41	6.287480888201559E-5
42-43	1.6674351242679025E-4
44-45	1.5634131671930833E-4
46-47	5.8384430413757616E-5
48-49	3.5907536797719953E-4
50-51	3.084526343100644E-4
52-53	4.6599775675849095E-4
54-55	0.0020340687254049947
56-57	0.0031298380563560095
58-59	0.001987457613644355
60-61	0.003162978672583554
62-63	0.0019586496112018346
64-65	0.002483592298003982
66-67	7.029210549363202E-4
68-69	4.928665251520989E-4
70-71	3.4303418918206084E-4
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	1.4612381377601249E-5
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	58.0
20-21	275.0
22-23	606.0
24-25	754.0
26-27	1776.0
28-29	4730.0
30-31	15148.0
32-33	19577.0
34-35	27402.0
36-37	43929.0
38-39	51291.0
40-41	50379.0
42-43	57797.0
44-45	69581.0
46-47	71013.0
48-49	66554.0
50-51	60847.0
52-53	59633.0
54-55	64165.0
56-57	66972.0
58-59	66557.0
60-61	68622.0
62-63	68034.0
64-65	73560.0
66-67	82330.0
68-69	84806.0
70-71	98330.0
72-73	70676.0
74-75	68949.0
76-77	72307.0
78-79	73173.0
80-81	73087.0
82-83	68991.0
84-85	68378.0
86-87	71095.0
88-89	73684.0
90-91	73409.0
92-93	73562.0
94-95	86064.0
96-97	272486.0
98-99	595642.0
100-101	5909226.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.91550580965216
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.65526048870149	76.13121792894208
2	7.150616039275434	12.1439635565137
3	1.8034796239603885	4.59430153457993
4	0.6407780026233733	2.1764795281784943
5	0.2876481172222321	1.2212892684559977
6	0.15746012295212367	0.8022483591197761
7	0.08909290632082992	0.529575844299966
8	0.05249555405109685	0.35661492200054584
9	0.03828344905273571	0.29257725964059916
>10	0.12259895374257858	1.5521696400626648
>50	0.0017015887555247263	0.09403753032369809
>100	5.207322752255886E-4	0.06404119640152625
>500	6.442106696429683E-5	0.04148343148104167
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.2407821225920695E-5	0.0	1.1203910612960348E-5	0.0
3	0.0	2.2407821225920695E-5	0.0	2.2407821225920695E-5	0.0
4	0.0	5.601955306480174E-5	0.0	3.361173183888104E-5	0.0
5	0.0	5.601955306480174E-5	0.0	4.481564245184139E-5	0.0
6	0.0	5.601955306480174E-5	0.0	4.481564245184139E-5	0.0
7	0.0	5.601955306480174E-5	0.0	5.601955306480174E-5	0.0
8	0.0	6.722346367776208E-5	0.0	7.842737429072244E-5	0.0
9	0.0	1.1203910612960348E-4	0.0	7.842737429072244E-5	0.0
10-11	0.0	1.1203910612960348E-4	0.0	1.1764106143608364E-4	1.1203910612960348E-5
12-13	0.0	1.1203910612960348E-4	0.0	1.4565083796848452E-4	1.1203910612960348E-5
14-15	0.0	1.680586591944052E-4	0.0	1.7926256980736556E-4	1.1203910612960348E-5
16-17	0.0	1.7926256980736556E-4	0.0	1.9046648042032592E-4	1.1203910612960348E-5
18-19	0.0	1.8486452511384574E-4	0.0	2.352821228721673E-4	2.2407821225920695E-5
20-21	0.0	1.9046648042032592E-4	0.0	2.744958100175285E-4	2.2407821225920695E-5
22-23	0.0	1.9046648042032592E-4	0.0	3.3051536308233024E-4	2.2407821225920695E-5
24-25	0.0	2.1847625695272678E-4	0.0	3.641270949212113E-4	3.361173183888104E-5
26-27	0.0	2.352821228721673E-4	0.0	4.1454469267953285E-4	3.361173183888104E-5
28-29	0.0	2.408840781786475E-4	0.0	6.106131284063389E-4	3.921368714536122E-5
30-31	0.0	3.081075418564096E-4	0.0	0.0014621103349913254	5.041759775832156E-5
32-33	0.0	3.2491340777585006E-4	0.0	0.003439600558178827	5.601955306480174E-5
34-35	0.0	3.7533100553417166E-4	0.0	0.005994092177933785	6.722346367776208E-5
36-37	0.0	4.4255446921193374E-4	0.0	0.009786615920420863	6.722346367776208E-5
38-39	0.0	4.705642457443346E-4	0.0	0.015237318433626072	8.40293295972026E-5
40-41	0.0	5.321857541156164E-4	0.0	0.021293032119931138	1.0083519551664312E-4
42-43	0.0	5.545935753415371E-4	0.0	0.026855773739265953	1.0083519551664312E-4
44-45	0.0	6.274189943257795E-4	0.0	0.03367335334725233	1.0083519551664312E-4
46-47	0.0	6.722346367776208E-4	0.0	0.041196779323855195	1.064371508231233E-4
48-49	0.0	6.834385473905812E-4	0.0	0.048484923177585904	1.2324301674256384E-4
50-51	0.0	7.058463686165019E-4	0.0	0.05620441758991558	1.2324301674256384E-4
52-53	0.0	7.058463686165019E-4	0.0	0.06377826116427678	1.3444692735552417E-4
54-55	0.0	7.170502792294623E-4	0.0	0.07120085194536302	1.3444692735552417E-4
56-57	0.0	7.282541898424226E-4	0.0	0.07865705445828812	1.3444692735552417E-4
58-59	0.0	7.338561451489028E-4	0.0	0.0858947807142605	1.3444692735552417E-4
60-61	0.0	7.842737429072243E-4	0.0	0.09276837987531168	1.3444692735552417E-4
62-63	0.0	7.954776535201846E-4	0.0	0.09915460892469907	1.3444692735552417E-4
64-65	0.0	8.963128490368278E-4	0.0	0.10560806143776424	1.3444692735552417E-4
66-67	0.0	9.075167596497882E-4	0.0	0.1118654455151026	1.3444692735552417E-4
68-69	0.0	9.243226255692288E-4	0.0	0.11829088825163535	1.3444692735552417E-4
70-71	0.0	9.411284914886692E-4	0.0	0.12351191059727487	1.3444692735552417E-4
72-73	0.0	9.411284914886692E-4	0.0	0.12733244411629435	1.5685474858144488E-4
74-75	0.0	9.523324021016295E-4	0.0	0.1303126843393418	1.6805865919440523E-4
76-77	0.0	9.803421786340305E-4	0.0	0.13348899299811606	1.7926256980736556E-4
78-79	0.0	9.97148044553471E-4	0.0	0.1358530181374507	1.7926256980736556E-4
80-81	0.0	9.97148044553471E-4	0.0	0.1366204860144385	1.7926256980736556E-4
82-83	0.0	9.97148044553471E-4	0.0	0.13716947763447354	1.9046648042032592E-4
84-85	0.0	0.0010083519551664312	0.0	0.1374047597573457	1.9046648042032592E-4
86-87	0.0	0.0010419636870053124	0.0	0.1374999929975559	1.9046648042032592E-4
88	0.0	0.0011091871506830745	0.0	0.13751679886347531	1.9046648042032592E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11180	0.0	48.221058	1
GTATCAA	20165	0.0	35.773987	1
CTTATAC	7515	0.0	34.78726	1
ATCAACG	24515	0.0	29.30399	3
TCAACGC	24745	0.0	29.08349	4
CAACGCA	25270	0.0	28.411535	5
TATCAAC	25665	0.0	28.224333	2
AACGCAG	26015	0.0	27.646317	6
TATACAC	11355	0.0	26.941816	3
TTATACA	11470	0.0	24.508116	2
ACGCAGA	29805	0.0	24.073393	7
CGCAGAG	30715	0.0	23.415882	8
TACACAT	15460	0.0	20.203255	5
GCAGAGT	35680	0.0	20.181463	9
GTACATG	25145	0.0	20.129875	1
TACATGG	25535	0.0	19.805672	2
TGGTATC	5690	0.0	19.776592	2
ACACATC	14235	0.0	19.626781	6
GTGGTAT	5800	0.0	19.62283	1
ACATCTC	14600	0.0	19.458466	8
>>END_MODULE