FastQCFastQC Report
Fri 27 May 2016
SRR2138737_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138737_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9444452
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA137900.050.557861
GTATCAA254200.037.772931
TCAACGC326200.029.9067364
ATCAACG328750.029.6098233
TATCAAC335250.029.2904322
CAACGCA333600.029.1793465
AACGCAG344400.028.3007556
TGGTATC62200.025.5340272
GTGGTAT65150.024.90211
ACGCAGA396150.024.5714327
CGCAGAG407950.023.8606998
CTTATAC61300.023.2623061
GCAGAGT460900.021.2028569
GTACATG357400.021.1917021
TACATGG365400.021.0899432
ACATGGG358900.020.8414253
GAGTACT256500.019.69863512-13
TATACAC93800.019.6173883
CATGGGG250050.018.9181944
AGAGTAC425500.018.59723310-11
Produced by FastQC (version 0.11.5)