##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138737_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9444452
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	22.79110159064814	18.0	18.0	30.0	18.0	33.0
2	30.817532769503195	32.0	29.0	33.0	27.0	33.0
3	31.57109316665488	33.0	31.0	33.0	29.0	33.0
4	32.16796898327187	33.0	33.0	33.0	31.0	34.0
5	32.47752055916002	33.0	33.0	34.0	31.0	34.0
6	36.90402206501764	38.0	37.0	38.0	35.0	38.0
7	37.10273089428587	38.0	38.0	38.0	36.0	38.0
8	37.192405340193375	38.0	38.0	38.0	36.0	38.0
9	37.31292922024486	38.0	38.0	38.0	37.0	38.0
10-11	37.342183485076745	38.0	38.0	38.0	37.0	38.0
12-13	37.366432271560065	38.0	38.0	38.0	37.0	38.0
14-15	37.35759782568644	38.0	38.0	38.0	37.0	38.0
16-17	37.3610571582131	38.0	38.0	38.0	37.0	38.0
18-19	37.250632117141365	38.0	38.0	38.0	37.0	38.0
20-21	37.352726665201246	38.0	38.0	38.0	37.0	38.0
22-23	37.36790806687944	38.0	38.0	38.0	37.0	38.0
24-25	37.373603024429336	38.0	38.0	38.0	37.0	38.0
26-27	37.34562696685987	38.0	38.0	38.0	37.0	38.0
28-29	37.32133237105608	38.0	38.0	38.0	37.0	38.0
30-31	37.327032116783656	38.0	38.0	38.0	37.0	38.0
32-33	37.31362897951442	38.0	38.0	38.0	37.0	38.0
34-35	37.29516612576222	38.0	38.0	38.0	37.0	38.0
36-37	37.269169009385756	38.0	38.0	38.0	37.0	38.0
38-39	37.232993193585614	38.0	38.0	38.0	37.0	38.0
40-41	37.24019500981467	38.0	38.0	38.0	37.0	38.0
42-43	37.215307382787756	38.0	38.0	38.0	37.0	38.0
44-45	37.18053390771122	38.0	38.0	38.0	37.0	38.0
46-47	37.14285977047268	38.0	38.0	38.0	36.0	38.0
48-49	37.109500402529726	38.0	38.0	38.0	36.0	38.0
50-51	37.08078805213323	38.0	38.0	38.0	36.0	38.0
52-53	37.062442632996195	38.0	38.0	38.0	36.0	38.0
54-55	37.04504950111261	38.0	38.0	38.0	36.0	38.0
56-57	37.035462842902085	38.0	38.0	38.0	36.0	38.0
58-59	37.02332852556263	38.0	38.0	38.0	36.0	38.0
60-61	37.021221283768384	38.0	38.0	38.0	36.0	38.0
62-63	37.017304461053	38.0	38.0	38.0	36.0	38.0
64-65	37.023737065160695	38.0	38.0	38.0	36.0	38.0
66-67	37.017328214365534	38.0	38.0	38.0	36.0	38.0
68-69	37.01945707907717	38.0	38.0	38.0	36.0	38.0
70-71	37.01439086736892	38.0	38.0	38.0	36.0	38.0
72-73	36.98862151035689	38.0	38.0	38.0	36.0	38.0
74-75	37.008333832077795	38.0	38.0	38.0	36.0	38.0
76-77	36.999724848536204	38.0	38.0	38.0	36.0	38.0
78-79	36.98878796786091	38.0	38.0	38.0	36.0	38.0
80-81	36.96316442081434	38.0	38.0	38.0	36.0	38.0
82-83	36.95254126601707	38.0	38.0	38.0	36.0	38.0
84-85	36.935986673183336	38.0	38.0	38.0	35.5	38.0
86-87	36.89981274316855	38.0	38.0	38.0	35.0	38.0
88-89	36.87825312352594	38.0	38.0	38.0	35.0	38.0
90-91	36.88442488804698	38.0	38.0	38.0	35.0	38.0
92-93	36.84983671054018	38.0	38.0	38.0	35.0	38.0
94-95	36.787880151976104	38.0	38.0	38.0	35.0	38.0
96-97	36.80080672671242	38.0	38.0	38.0	35.0	38.0
98-99	36.82788039071255	38.0	38.0	38.0	35.0	38.0
100	35.20176263319476	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	4.0
19	39.0
20	110.0
21	294.0
22	867.0
23	2191.0
24	5083.0
25	9750.0
26	17016.0
27	27659.0
28	42370.0
29	60864.0
30	83604.0
31	109326.0
32	141091.0
33	189208.0
34	281840.0
35	505130.0
36	1553561.0
37	6414444.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.983277801613056	19.445617384682564	17.16836508883734	22.402739724867043
2	14.641738874844194	20.924210319455273	41.934577040573664	22.499473765126872
3	18.622223925750273	26.344757747723214	30.064539477780183	24.968478848746333
4	12.675981623920581	18.006942065034583	38.37759988615538	30.93947642488945
5	13.43443958666651	37.65964047737286	35.339780330552436	13.566139605408193
6	29.96756294308453	37.61022484645736	18.595873023772175	13.82633918668593
7	26.4905286712801	32.72632787231359	22.985031104507822	17.798112351898485
8	24.399658127332323	36.8201987791351	20.936693838880224	17.84344925465236
9	25.66591476138584	16.998148754422175	21.310267657668227	36.02566882652376
10-11	24.50100863448721	26.82230265980493	28.38742258417958	20.28926612152828
12-13	25.32992124158408	24.354226624713284	28.705024781218093	21.610827352484545
14-15	22.413555598567285	25.53779721682105	26.52620819079815	25.522438993813513
16-17	21.770712583429933	28.750561705432986	27.227090571268718	22.25163513986836
18-19	21.81789901626902	27.58422087379977	29.17661607047185	21.421264039459356
20-21	22.7207965591478	26.757456224704292	29.095347622489072	21.42639959365884
22-23	22.494613893995076	26.683353112182758	28.995261815732803	21.82677117808936
24-25	22.156011342303227	26.89929647798217	29.10193239161673	21.842759788097872
26-27	22.035537678775093	27.045482088325475	29.088514703752615	21.83046552914682
28-29	21.906558126353215	26.960947561643955	29.201492031714736	21.931002280288087
30-31	22.22166385036757	26.995392972294102	28.97033830241242	21.812604874925913
32-33	22.135785326694513	27.008529285172898	28.949127092809245	21.906558295323343
34-35	22.20546680425654	26.99581414833534	28.951517904940914	21.847201142467195
36-37	22.11656707601563	26.984848452421208	28.900118995133184	21.998465476429974
38-39	21.957149514103016	27.041230856176313	29.035049221883135	21.966570407837537
40-41	22.20856519749321	26.909718519246734	28.94901697030735	21.93269931295271
42-43	22.16780624999047	26.974722755620768	28.707509658369034	22.14996133601973
44-45	22.14174025106724	27.16770548643352	28.5766737884605	22.113880474038744
46-47	22.33729005313223	27.08268029015072	28.255631181100426	22.324398475616626
48-49	22.26120486803117	27.184390892297632	28.186736383202888	22.367667856468305
50-51	22.218234223518145	27.42755502569281	27.982764856580562	22.371445894208478
52-53	22.421632954470695	27.403516770370622	27.833260484975032	22.34158979018365
54-55	22.445828147026056	27.34582033463166	27.743748423488597	22.464603094853693
56-57	22.45831515096165	27.536994331141095	27.61673024892189	22.38796026897537
58-59	22.321864930581185	27.57001196542948	27.800094438182455	22.30802866580688
60-61	22.442876384522524	27.70027716132562	27.64734559989152	22.209500854260337
62-63	22.3368959817651	27.811710628185416	27.613532970118104	22.23786041993138
64-65	22.324006461912145	27.69472501546734	27.5979228408735	22.38334568174701
66-67	22.436749884244417	27.709791567694893	27.44853015997452	22.404928388086176
68-69	22.41108648439655	27.645243249050626	27.599958144110847	22.343712122441975
70-71	22.368654555930636	27.34426344306135	27.736941471464565	22.55014052954345
72-73	22.48309282146856	27.351904588028013	27.588526248758598	22.576476341744822
74-75	22.337665201107637	27.49999624172804	27.579803146725943	22.582535410438375
76-77	22.514374178828298	27.40220179259894	27.54064977697757	22.542774251595187
78-79	22.47717967627419	27.394401359455422	27.464198020600637	22.66422094366975
80-81	22.488177244296427	27.49257284911702	27.400219706116157	22.61903020047039
82-83	22.57940489741424	27.428880955530026	27.49415238783572	22.497561759220012
84-85	22.625129170999738	27.4259414126988	27.37401023645273	22.57491917984873
86-87	22.465939173721402	27.529197243638304	27.47761740811161	22.527246174528685
88-89	22.596273760220665	27.430164893231296	27.414074711074765	22.559486635473274
90-91	22.60644581446398	27.49865406239669	27.408730879818517	22.486169243320816
92-93	22.66419718259375	27.51195056179714	27.357794778814227	22.46605747679489
94-95	22.49179178757602	27.55052086715458	27.369458527339813	22.588228817929583
96-97	22.64744705518649	27.602700569467544	27.431236955610395	22.318615419735572
98-99	23.682334423193073	29.51814996789212	27.636535767329846	19.162979841584963
100	22.627388073480738	30.385853454572416	21.623916390064185	25.362842081882665
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3106.0
1	2724.5
2	2664.5
3	3429.5
4	4784.5
5	6690.0
6	7987.0
7	8309.5
8	8103.5
9	8172.0
10	8700.0
11	10177.5
12	12962.5
13	19628.5
14	30135.5
15	40495.5
16	48146.5
17	50783.0
18	50024.0
19	45237.5
20	39442.0
21	36185.5
22	35873.0
23	40205.0
24	48327.0
25	60987.0
26	78095.0
27	96407.5
28	114790.0
29	135242.0
30	155710.5
31	174903.5
32	196418.0
33	218332.5
34	239374.5
35	260079.5
36	282218.0
37	301447.0
38	313380.5
39	323911.5
40	334886.0
41	342724.0
42	347433.0
43	360200.0
44	371265.5
45	375893.5
46	377486.5
47	375767.5
48	377759.5
49	377393.0
50	372658.0
51	367201.0
52	359936.0
53	345725.0
54	328242.0
55	310702.5
56	289058.0
57	262457.5
58	235762.5
59	208381.0
60	177061.5
61	143955.0
62	113218.0
63	87866.0
64	66764.5
65	49708.0
66	36429.5
67	27147.5
68	20446.5
69	15117.5
70	10956.5
71	7539.0
72	5081.0
73	3328.5
74	2181.5
75	1420.0
76	896.0
77	542.0
78	331.0
79	214.5
80	153.5
81	110.5
82	75.0
83	49.5
84	33.5
85	22.0
86	14.0
87	8.0
88	5.5
89	5.0
90	3.0
91	1.5
92	2.0
93	2.0
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0027741154277664813
6	7.411758776475331E-5
7	1.0588226823536188E-5
8	0.0
9	0.0
10-11	0.0
12-13	1.0588226823536188E-5
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	5.848710035099704E-5
34-35	1.5458315054319986E-4
36-37	1.7121572582198528E-4
38-39	1.7216909221532607E-4
40-41	1.1368309797052393E-4
42-43	1.9065042680911051E-4
44-45	1.9206033548001915E-4
46-47	6.64228542000084E-5
48-49	3.238152674097185E-4
50-51	4.903561044938714E-4
52-53	4.321413793736759E-4
54-55	0.0021191529848613435
56-57	0.003342423493715782
58-59	0.0019732440907014744
60-61	0.0036393893456876905
62-63	0.002231254033420753
64-65	0.0028463397234754105
66-67	8.248834460764359E-4
68-69	8.633766624788845E-4
70-71	4.1155898420362904E-4
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	6.695872275968501E-6
90-91	0.0
92-93	0.0
94-95	3.457762531657545E-5
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	1028.0
20-21	3154.0
22-23	1892.0
24-25	2030.0
26-27	3272.0
28-29	6645.0
30-31	17604.0
32-33	21997.0
34-35	30592.0
36-37	48631.0
38-39	57642.0
40-41	56064.0
42-43	63444.0
44-45	77244.0
46-47	78695.0
48-49	73285.0
50-51	92515.0
52-53	62367.0
54-55	67280.0
56-57	71063.0
58-59	71894.0
60-61	71109.0
62-63	68263.0
64-65	72849.0
66-67	79317.0
68-69	83223.0
70-71	87916.0
72-73	73430.0
74-75	71474.0
76-77	74279.0
78-79	77527.0
80-81	76679.0
82-83	71640.0
84-85	69846.0
86-87	72380.0
88-89	77550.0
90-91	79895.0
92-93	78462.0
94-95	87818.0
96-97	282148.0
98-99	635788.0
100-101	6244521.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.94998822991234
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.3267842186727	74.98982483773493
2	7.33308855817232	12.312253962949422
3	1.83156864332538	4.612804981483264
4	0.6972705525541264	2.3414341871993356
5	0.3061033484108507	1.2848686248113836
6	0.15835663702320332	0.7976422688535852
7	0.09456732109801999	0.5557247845179184
8	0.06288823809532937	0.42235734783222606
9	0.042288742287296524	0.3195125475548706
>10	0.1414146579361141	1.8914874683457976
>50	0.004145997389741757	0.2361625062196079
>100	0.001454910689072079	0.17747330705194472
>500	2.2725021513949222E-5	0.010836106839364678
>1k	4.544932432367952E-5	0.0476170686063885
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.0588226823536188E-5	0.0	0.0	0.0
3	0.0	1.0588226823536188E-5	0.0	0.0	0.0
4	0.0	5.2941134117680944E-5	0.0	0.0	0.0
5	0.0	5.2941134117680944E-5	0.0	0.0	0.0
6	0.0	6.352936094121714E-5	0.0	0.0	0.0
7	0.0	6.352936094121714E-5	0.0	0.0	0.0
8	0.0	9.52940414118257E-5	0.0	0.0	0.0
9	0.0	2.1176453647072377E-4	0.0	0.0	0.0
10-11	1.0588226823536188E-5	2.1176453647072377E-4	0.0	0.0	0.0
12-13	1.0588226823536188E-5	2.1176453647072377E-4	0.0	0.0	0.0
14-15	1.0588226823536188E-5	3.3352914494138994E-4	0.0	0.0	0.0
16-17	1.0588226823536188E-5	3.494114851766942E-4	0.0	0.0	0.0
18-19	1.0588226823536188E-5	3.8117616564730273E-4	0.0	5.294113411768094E-6	0.0
20-21	1.0588226823536188E-5	4.1294084611791134E-4	0.0	1.0588226823536188E-5	0.0
22-23	1.0588226823536188E-5	5.188231143532732E-4	0.0	2.6470567058840472E-5	0.0
24-25	1.0588226823536188E-5	5.505877948238818E-4	0.0	4.235290729414475E-5	1.0588226823536188E-5
26-27	1.0588226823536188E-5	5.611760216474179E-4	0.0	8.999992800005761E-5	1.0588226823536188E-5
28-29	1.0588226823536188E-5	5.611760216474179E-4	0.0	2.8588212423547705E-4	1.0588226823536188E-5
30-31	1.0588226823536188E-5	6.141171557650989E-4	0.0	9.688227543535612E-4	1.0588226823536188E-5
32-33	1.0588226823536188E-5	6.352936094121713E-4	0.0	0.0028111742216488577	1.0588226823536188E-5
34-35	1.0588226823536188E-5	7.411758776475332E-4	0.0	0.005129995896003284	1.0588226823536188E-5
36-37	1.0588226823536188E-5	8.470581458828951E-4	0.0	0.008931169325652775	1.0588226823536188E-5
38-39	1.0588226823536188E-5	8.682345995299674E-4	0.0	0.014722929398127069	1.0588226823536188E-5
40-41	1.0588226823536188E-5	8.947051665888078E-4	0.0	0.020821748048483912	1.0588226823536188E-5
42-43	1.0588226823536188E-5	9.211757336476484E-4	0.0	0.026285273089428587	1.0588226823536188E-5
44-45	1.0588226823536188E-5	9.952933214124019E-4	0.0	0.032071739048491116	1.0588226823536188E-5
46-47	1.0588226823536188E-5	0.0010482344555300827	0.0	0.03896996882402494	1.0588226823536188E-5
48-49	1.0588226823536188E-5	0.0010482344555300827	0.0	0.046074669022617726	1.0588226823536188E-5
50-51	1.0588226823536188E-5	0.0011276461567066042	0.0	0.0530470163859163	1.0588226823536188E-5
52-53	1.0588226823536188E-5	0.0011435284969419084	0.0	0.060035246089450184	1.0588226823536188E-5
54-55	1.0588226823536188E-5	0.0011488226103536763	0.0	0.0665205350188661	1.0588226823536188E-5
56-57	1.0588226823536188E-5	0.0011647049505889807	0.0	0.07161876623439878	1.0588226823536188E-5
58-59	1.0588226823536188E-5	0.001180587290824285	0.0	0.07701346780099047	1.0588226823536188E-5
60-61	1.0588226823536188E-5	0.0012282343115301978	0.0	0.0823022870993468	1.0588226823536188E-5
62-63	1.0588226823536188E-5	0.0012494107651772701	0.0	0.08783992972805621	1.0588226823536188E-5
64-65	1.0588226823536188E-5	0.0014399988480009215	0.0	0.0928058081082947	1.0588226823536188E-5
66-67	1.0588226823536188E-5	0.0014399988480009215	0.0	0.09765521599347426	1.0588226823536188E-5
68-69	1.0588226823536188E-5	0.001466469415059762	0.0	0.10255227089935975	1.0588226823536188E-5
70-71	1.0588226823536188E-5	0.00147176352847153	0.0	0.1072746200626569	1.0588226823536188E-5
72-73	1.0588226823536188E-5	0.0014770576418832982	0.0	0.11063638207912962	1.0588226823536188E-5
74-75	1.0588226823536188E-5	0.001514116435765675	0.0	0.11330461523866076	1.0588226823536188E-5
76-77	1.0588226823536188E-5	0.001567057569883356	0.0	0.11579284854219175	1.0588226823536188E-5
78-79	1.0588226823536188E-5	0.0015988222503539645	0.0	0.11766167057654589	1.0588226823536188E-5
80-81	1.0588226823536188E-5	0.0015988222503539645	0.0	0.11825990539207569	1.0588226823536188E-5
82-83	1.0588226823536188E-5	0.0016041163637657324	0.0	0.11868872857842891	1.0588226823536188E-5
84-85	1.0588226823536188E-5	0.0016199987040010368	0.0	0.11890049311489963	1.0588226823536188E-5
86-87	1.0588226823536188E-5	0.0016199987040010368	0.0	0.11896931658925261	1.0588226823536188E-5
88	1.0588226823536188E-5	0.001683528064942254	0.0	0.11897990481607615	1.0588226823536188E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	13790	0.0	50.55786	1
GTATCAA	25420	0.0	37.77293	1
TCAACGC	32620	0.0	29.906736	4
ATCAACG	32875	0.0	29.609823	3
TATCAAC	33525	0.0	29.290432	2
CAACGCA	33360	0.0	29.179346	5
AACGCAG	34440	0.0	28.300755	6
TGGTATC	6220	0.0	25.534027	2
GTGGTAT	6515	0.0	24.9021	1
ACGCAGA	39615	0.0	24.571432	7
CGCAGAG	40795	0.0	23.860699	8
CTTATAC	6130	0.0	23.262306	1
GCAGAGT	46090	0.0	21.202856	9
GTACATG	35740	0.0	21.191702	1
TACATGG	36540	0.0	21.089943	2
ACATGGG	35890	0.0	20.841425	3
GAGTACT	25650	0.0	19.698635	12-13
TATACAC	9380	0.0	19.617388	3
CATGGGG	25005	0.0	18.918194	4
AGAGTAC	42550	0.0	18.597233	10-11
>>END_MODULE