FastQCFastQC Report
Fri 27 May 2016
SRR2138736_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138736_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7430444
Sequences flagged as poor quality0
Sequence length19-101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA99450.059.085231
GTATCAA190450.042.8219381
ATCAACG242600.033.1361583
TCAACGC244500.032.877694
TATCAAC247600.032.6172522
CAACGCA255100.030.1038595
AACGCAG265300.029.0135736
GTGGTAT42500.026.3769971
ACGCAGA301150.025.5678187
CGCAGAG305200.025.278338
TGGTATC42950.025.1218662
GCAGAGT346650.021.9713529
GTACATG240650.021.6805531
TACATGG247950.020.8161892
GAGTACT199350.020.74900412-13
ACATGGG254450.019.946723
AGAGTAC317950.019.5466910-11
CAGAGTA336500.019.51745610-11
GTACTTT224900.018.4652614-15
AGTACTT219150.018.14278812-13
Produced by FastQC (version 0.11.5)