FastQCFastQC Report
Fri 27 May 2016
SRR2138735_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138735_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8875067
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA104500.057.8216171
GTATCAA193750.041.70271
TCAACGC241050.032.6808474
ATCAACG241500.032.621563
TATCAAC250150.031.7384722
CAACGCA248900.029.8625455
AACGCAG258600.028.8007746
ACGCAGA292900.025.4507377
CGCAGAG302500.024.6519418
GTGGTAT48750.023.8283441
GCAGAGT347300.021.174489
TGGTATC50000.021.1716772
GTACATG225600.020.1187731
GAGTACT213350.019.86157412-13
TACATGG229800.019.5064742
AGAGTAC313950.019.36694110-11
CAGAGTA337150.019.18091210-11
ACATGGG234450.018.4734133
GTACTTT239000.017.71015514-15
AGTACTT235300.017.5250212-13
Produced by FastQC (version 0.11.5)