##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138735_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8875067
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.763872317808982	33.0	31.0	34.0	30.0	34.0
2	31.479202128840267	33.0	31.0	34.0	28.0	34.0
3	31.79412132888687	33.0	31.0	34.0	30.0	34.0
4	32.9977404114245	37.0	35.0	37.0	27.0	37.0
5	34.43523029178259	37.0	35.0	37.0	28.0	37.0
6	35.080538546920266	37.0	35.0	37.0	32.0	37.0
7	35.21558259785532	37.0	35.0	37.0	32.0	37.0
8	35.35861036316684	37.0	35.0	37.0	32.0	37.0
9	37.118591330071084	39.0	37.0	39.0	32.0	39.0
10-11	36.99924423105763	39.0	37.0	39.0	32.0	39.0
12-13	36.891906787858616	39.0	37.0	39.0	32.0	39.0
14-15	38.241399529716226	40.0	38.0	41.0	32.0	41.0
16-17	38.23751589706309	40.0	38.0	41.0	32.0	41.0
18-19	38.17403716501521	40.0	38.0	41.0	32.0	41.0
20-21	38.23379352276617	40.0	38.0	41.0	32.0	41.0
22-23	38.27722699651038	40.0	38.0	41.0	32.0	41.0
24-25	38.164432910118656	40.0	38.0	41.0	32.0	41.0
26-27	38.07636624565771	40.0	38.0	41.0	32.0	41.0
28-29	38.085552188461676	40.0	38.0	41.0	32.0	41.0
30-31	38.035468649423905	40.0	37.5	41.0	32.0	41.0
32-33	37.93868967471275	40.0	37.0	41.0	32.0	41.0
34-35	37.83359266926372	40.0	37.0	41.0	31.0	41.0
36-37	37.79652764777488	40.0	37.0	41.0	31.0	41.0
38-39	37.73930986423619	40.0	37.0	41.0	31.0	41.0
40-41	37.6474168686478	40.0	37.0	41.0	31.0	41.0
42-43	37.62095654932752	40.0	37.0	41.0	31.0	41.0
44-45	37.55901096543788	40.0	36.5	41.0	30.5	41.0
46-47	37.42516990630092	40.0	36.0	41.0	30.0	41.0
48-49	37.60104922742123	40.0	37.0	41.0	31.0	41.0
50-51	37.5654428587564	40.0	36.0	41.0	31.0	41.0
52-53	37.49786159691761	40.0	36.0	41.0	30.5	41.0
54-55	37.37380793628622	39.5	36.0	41.0	30.0	41.0
56-57	37.24284575731794	39.0	35.5	41.0	30.0	41.0
58-59	37.12846674147332	39.0	35.0	41.0	30.5	41.0
60-61	36.92028183632467	39.0	35.0	41.0	30.5	41.0
62-63	36.73040483219401	38.5	35.0	41.0	30.0	41.0
64-65	36.46726920319513	38.0	35.0	40.0	30.0	41.0
66-67	36.24453806044021	37.5	35.0	40.0	30.0	41.0
68-69	35.99382394492545	37.0	34.0	40.0	30.0	41.0
70-71	35.74278056795069	36.5	34.0	39.0	30.0	41.0
72-73	35.43852054059596	36.0	34.0	39.0	30.0	41.0
74-75	35.25669468240754	36.0	34.0	39.0	30.0	40.5
76-77	35.063005616894785	35.0	34.0	37.5	30.0	40.0
78-79	34.80570311009038	35.0	34.0	37.0	30.0	39.0
80-81	34.556586728724284	35.0	34.0	37.0	30.0	39.0
82-83	34.31279899456069	35.0	34.0	36.0	30.0	38.5
84-85	34.103051937944684	35.0	34.0	36.0	30.0	37.0
86-87	33.920146649716926	35.0	34.0	36.0	29.5	37.0
88-89	33.783893045704076	35.0	34.0	35.0	29.0	36.5
90-91	33.65413829774086	35.0	34.0	35.0	29.0	36.0
92-93	33.61333218778664	35.0	34.0	35.0	29.0	36.0
94-95	33.59068472910435	35.0	34.0	35.0	29.5	36.0
96-97	33.59766554662022	35.0	34.0	35.0	30.0	36.0
98-99	33.629719958527915	35.0	34.0	35.0	30.0	35.0
100-101	33.246210976298904	34.5	33.0	35.0	29.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	2.0
16	15.0
17	98.0
18	603.0
19	2249.0
20	6508.0
21	13609.0
22	23272.0
23	34712.0
24	51521.0
25	75256.0
26	106963.0
27	144465.0
28	158245.0
29	136195.0
30	163139.0
31	198866.0
32	246317.0
33	321258.0
34	438230.0
35	621612.0
36	951644.0
37	1663882.0
38	2243087.0
39	1213198.0
40	60120.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.61527648185642	20.326178946029366	14.757511126394876	24.301033445719337
2	14.320133924220446	20.786804795129193	41.63272887798334	23.260332402667018
3	17.56758888577386	26.046319492083867	30.71160002055319	25.674491601589082
4	12.153638088800921	18.153931177418816	38.73927770787446	30.953153025905806
5	13.169904173931425	37.57145603207558	35.57607302639155	13.682566767601445
6	29.81784812554527	38.16275550815555	18.666933385684832	13.352462980614346
7	26.227550106702324	32.797212609526966	23.167021965407823	17.808215318362887
8	24.1423137391041	37.089182199323474	21.003511995647102	17.764992065925327
9	25.274055041091636	17.04034752736945	21.3393581686712	36.34623926286771
10-11	23.97453103673231	26.881123273242384	29.00273078698319	20.141614903042118
12-13	24.77232250931901	24.483682378542053	29.100619097422907	21.643376014716022
14-15	21.97074600223852	25.841592854493783	26.69554759654878	25.49211354671892
16-17	21.52760942986673	29.007195616163116	27.37647360329481	22.088721350675343
18-19	21.59254429106976	28.061466883934838	29.186110996169884	21.159877828825522
20-21	22.294290346230333	27.171868417000045	29.186468903404943	21.34737233336468
22-23	22.0711951272522	27.108622045504347	29.04640437349677	21.77377845374668
24-25	21.903325498128893	27.253515534500522	29.0445632947575	21.798595672613086
26-27	21.822970003512992	27.42440313297069	29.008091861663775	21.74453500185254
28-29	21.785662412199795	27.322193378155756	29.094559558217814	21.797584651426636
30-31	22.03375758552658	27.236547707403663	28.975654650470645	21.75404005659911
32-33	21.785439695109158	27.439677551539393	28.928948573457813	21.845934179893643
34-35	21.987821407986356	27.39390954543619	28.9434511174992	21.67481792907825
36-37	21.928965396188595	27.32409478843522	28.861921612550162	21.885018202826018
38-39	21.833958202714427	27.39277023642643	28.970146105961497	21.80312545489765
40-41	22.04210883988625	27.28357910207679	28.8201192098558	21.85419284818116
42-43	21.938458808938226	27.38721839300261	28.665070437031538	22.00925236102762
44-45	22.00722529537495	27.49948472189454	28.500793601371505	21.992496381359004
46-47	22.153518874860826	27.480070157436415	28.1799980490185	22.18641291868426
48-49	22.04095829828927	27.567776696669505	28.153522111429464	22.237742893611763
50-51	22.063183038623542	27.708983346067463	28.02582778626453	22.20200582904447
52-53	22.180309759871715	27.693865149011327	27.968149028683527	22.15767606243344
54-55	22.165684673587744	27.628961980481147	27.93821559239669	22.267137753534417
56-57	22.156245036138145	27.74683825051747	27.917804103082105	22.179112610262276
58-59	22.087888980382335	27.783274343521434	27.96833308415339	22.16050359194284
60-61	22.21147830470953	27.786816339151716	27.89142820416236	22.110277151976394
62-63	22.15747698277376	27.90627585134328	27.86083147486229	22.075415691020673
64-65	22.179472908222266	27.79783382000307	27.81802614858763	22.20466712318703
66-67	22.17773572899855	27.813028480398792	27.738402262913457	22.270833527689195
68-69	22.125822682395448	27.77202904225228	27.814562392265362	22.2875858830869
70-71	22.150555046320676	27.566620957008297	27.89741021722824	22.38541377944279
72-73	22.21475980794372	27.623016694458375	27.794350070992056	22.367873426605847
74-75	22.086099846827167	27.782994708331394	27.761504168990513	22.36940127585093
76-77	22.21527761056902	27.72562968862739	27.738947020421172	22.320145680382414
78-79	22.15018361437686	27.700829207255882	27.708901596009	22.440085582358254
80-81	22.158637142491123	27.754548724742957	27.719067144692573	22.367746988073353
82-83	22.209570953407066	27.730452718591568	27.76623873400631	22.293737593995047
84-85	22.222455204705117	27.73739061737181	27.731396613493708	22.308757564429364
86-87	22.164621621721924	27.699310876807132	27.817792733188938	22.318274768282006
88-89	22.1813076797492	27.71190182780921	27.773128088108074	22.333662404333516
90-91	22.195096015476057	27.77417885690548	27.76137611178659	22.269349015831878
92-93	22.201000036453422	27.7346445129427	27.79710335033581	22.267252100268063
94-95	22.06609518338532	27.761692145743588	27.816913143953197	22.355299526917896
96-97	22.138143014732993	27.75540598792562	27.815648646326785	22.290802351014598
98-99	22.39030831371481	28.314224395915133	27.887394298074575	21.40807299229548
100-101	23.028413794647825	30.94821758599961	25.042774996868417	20.980593622484147
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3201.0
1	2518.0
2	2580.0
3	3353.5
4	3942.5
5	5849.5
6	7790.0
7	10051.5
8	12074.5
9	12061.0
10	10180.0
11	8260.0
12	8753.5
13	11945.0
14	20050.0
15	31238.0
16	40320.0
17	45444.0
18	46575.5
19	44571.5
20	40753.5
21	37334.5
22	36518.5
23	40357.5
24	48948.5
25	61494.0
26	76951.0
27	93516.5
28	112169.5
29	132621.5
30	152132.0
31	172084.0
32	193057.0
33	214571.5
34	235016.5
35	253265.0
36	273823.5
37	291180.0
38	304548.5
39	318588.5
40	331072.0
41	342120.5
42	348982.0
43	354090.5
44	360566.0
45	366266.5
46	368209.5
47	365435.0
48	360733.0
49	355615.5
50	348787.5
51	338719.0
52	324633.0
53	307177.0
54	289101.5
55	270422.0
56	249558.5
57	227022.5
58	203126.5
59	176383.5
60	148702.5
61	122445.0
62	98418.0
63	77044.5
64	58367.5
65	43857.5
66	33218.5
67	25811.5
68	20161.0
69	15365.5
70	11537.0
71	8496.5
72	6224.5
73	4460.0
74	3109.5
75	2144.5
76	1487.5
77	1012.0
78	688.0
79	475.0
80	339.5
81	243.5
82	186.5
83	135.0
84	90.0
85	66.0
86	54.0
87	43.0
88	31.5
89	25.0
90	19.0
91	13.0
92	10.5
93	5.5
94	1.0
95	1.5
96	2.0
97	1.5
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	0.0
2	1.7320094597595714
3	0.005983053423709365
4	8.5799915651341
5	3.267580965867638E-4
6	0.07670928005388579
7	0.025374456328048002
8	6.309811520296128E-4
9	2.816880142989343E-4
10-11	0.29054991922877876
12-13	0.304504743457148
14-15	7.323888371772292E-5
16-17	1.183089660055524E-4
18-19	0.4738048738110935
20-21	2.1975567451412302E-4
22-23	1.6909214315611386E-4
24-25	0.16620773514120496
26-27	0.1497978175122528
28-29	1.3547980766609105E-4
30-31	1.0173180309108694E-4
32-33	7.929239916088253E-5
34-35	3.9186332715817306E-4
36-37	2.9643887410463345E-4
38-39	0.0015186903502128603
40-41	0.0017410001677009765
42-43	9.978994796128267E-4
44-45	8.185911403296173E-4
46-47	0.0012746722100744781
48-49	0.0024172525626449458
50-51	3.4216345148077235E-4
52-53	0.001487922694794938
54-55	4.0227553863018473E-4
56-57	0.008623774116372024
58-59	1.1782032400589103E-4
60-61	0.0025157281509166542
62-63	0.0014712218895590982
64-65	0.0014336411477692797
66-67	0.004702922618715985
68-69	0.0018910574169221435
70-71	0.0024098607034849606
72-73	0.003508523390509663
74-75	7.098725457328691E-4
76-77	3.719911244270407E-4
78-79	8.407243599063063E-4
80-81	4.6295128681455997E-4
82-83	0.0015507265712806835
84-85	2.1886185340971943E-4
86-87	3.711242495439454E-4
88-89	1.4435028154800665E-4
90-91	2.9943581180736755E-4
92-93	0.0
94-95	0.0
96-97	3.0410217103101435E-5
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	986.0
20-21	2468.0
22-23	3151.0
24-25	4000.0
26-27	5284.0
28-29	8277.0
30-31	17614.0
32-33	22385.0
34-35	30029.0
36-37	43465.0
38-39	51141.0
40-41	53612.0
42-43	62656.0
44-45	77363.0
46-47	76497.0
48-49	70722.0
50-51	63490.0
52-53	62255.0
54-55	67407.0
56-57	71106.0
58-59	72900.0
60-61	72127.0
62-63	70783.0
64-65	74250.0
66-67	77427.0
68-69	79325.0
70-71	77377.0
72-73	72788.0
74-75	72853.0
76-77	76101.0
78-79	79255.0
80-81	78864.0
82-83	76249.0
84-85	75848.0
86-87	77454.0
88-89	80530.0
90-91	84081.0
92-93	84949.0
94-95	93009.0
96-97	145239.0
98-99	729613.0
100-101	5730137.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.60176608870833
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.48708664300983	80.14430364238339
2	6.076304096729433	10.645899403311038
3	1.3682828482492293	3.595919820465618
4	0.47934291452379235	1.6796514349757188
5	0.23636899436958914	1.0353170677693984
6	0.11768819458196705	0.6185816215903122
7	0.07185990030210442	0.4406537924195997
8	0.04292510796942743	0.30082522141362655
9	0.03064665979734933	0.24162313706708555
>10	0.08800834299280824	1.169524078058919
>50	0.001262024267808729	0.07663896162672644
>100	1.8555414756208402E-4	0.02974413233109581
>500	3.87190590524798E-5	0.021317686587389462
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	1.1267520571957373E-5	0.0
3	0.0	0.0	0.0	1.1267520571957373E-5	0.0
4	0.0	5.6337602859786864E-5	0.0	1.1267520571957373E-5	0.0
5	0.0	5.6337602859786864E-5	0.0	3.380256171587212E-5	0.0
6	2.2535041143914746E-5	5.6337602859786864E-5	0.0	3.380256171587212E-5	4.507008228782949E-5
7	2.2535041143914746E-5	5.6337602859786864E-5	0.0	3.380256171587212E-5	4.507008228782949E-5
8	2.2535041143914746E-5	6.760512343174424E-5	0.0	3.380256171587212E-5	4.507008228782949E-5
9	2.2535041143914746E-5	1.0140768514761635E-4	0.0	4.507008228782949E-5	4.507008228782949E-5
10-11	2.8168801429893432E-5	1.0140768514761635E-4	0.0	7.88726440037016E-5	4.507008228782949E-5
12-13	3.380256171587212E-5	1.0140768514761635E-4	0.0	7.88726440037016E-5	4.507008228782949E-5
14-15	4.507008228782949E-5	1.5211152772142452E-4	0.0	8.45064042896803E-5	5.6337602859786864E-5
16-17	6.760512343174424E-5	1.577452880074032E-4	0.0	9.014016457565898E-5	6.760512343174424E-5
18-19	6.760512343174424E-5	1.690128085793606E-4	0.0	9.577392486163767E-5	6.760512343174424E-5
20-21	6.760512343174424E-5	1.8028032915131797E-4	0.0	1.0704144543359503E-4	7.88726440037016E-5
22-23	6.760512343174424E-5	2.1971665115316877E-4	0.0	1.690128085793606E-4	1.0140768514761635E-4
24-25	7.323888371772292E-5	2.647867334409983E-4	0.0	2.3661793201110482E-4	1.3521024686348847E-4
26-27	7.88726440037016E-5	2.816880142989343E-4	0.0	3.549268980166572E-4	1.3521024686348847E-4
28-29	7.88726440037016E-5	2.816880142989343E-4	0.0	0.0013239336672049911	1.3521024686348847E-4
30-31	9.014016457565898E-5	3.154905760148064E-4	0.0	0.004326727899631631	1.3521024686348847E-4
32-33	1.295764865775098E-4	3.267580965867638E-4	0.0	0.011802727799125348	1.4084400714946716E-4
34-35	1.4084400714946716E-4	3.9436322001850803E-4	0.0	0.02312095221365653	1.4647776743544584E-4
36-37	1.4647776743544584E-4	4.507008228782949E-4	0.0	0.03932364679613123	1.577452880074032E-4
38-39	1.4647776743544584E-4	4.901371448801457E-4	0.0	0.0604502478685513	1.577452880074032E-4
40-41	1.4647776743544584E-4	6.535161931735276E-4	0.0	0.07977967940974418	1.633790482933819E-4
42-43	1.4647776743544584E-4	6.535161931735276E-4	0.0	0.09660772138396251	1.6901280857936057E-4
44-45	1.4647776743544584E-4	7.042200357473357E-4	0.0	0.11583011147972178	1.8028032915131797E-4
46-47	1.4647776743544584E-4	7.549238783211439E-4	0.0	0.13309758675624644	1.9154784972327533E-4
48-49	1.4647776743544584E-4	7.605576386071226E-4	0.0	0.14788620750694054	2.028153702952327E-4
50-51	1.4647776743544584E-4	8.450640428968029E-4	0.0	0.1595480912989164	2.028153702952327E-4
52-53	1.4647776743544584E-4	8.450640428968029E-4	0.0	0.1687536556062056	2.1408289086719006E-4
54-55	1.4647776743544584E-4	8.506978031827817E-4	0.0	0.17777893958434343	2.3098417172512614E-4
56-57	1.4647776743544584E-4	8.78866604612675E-4	0.0	0.18433100279693665	2.3661793201110482E-4
58-59	1.4647776743544584E-4	8.845003648986537E-4	0.0	0.1899985656446312	2.3661793201110482E-4
60-61	1.633790482933819E-4	9.014016457565898E-4	0.0	0.1949619084565784	2.3661793201110482E-4
62-63	1.8028032915131797E-4	9.014016457565898E-4	0.0	0.19962102821308278	2.591529731550196E-4
64-65	1.8028032915131797E-4	9.69006769188334E-4	0.0	0.20428578172987313	2.591529731550196E-4
66-67	1.8028032915131797E-4	0.0010366118926200783	0.0	0.2093730672681119	2.647867334409983E-4
68-69	1.8028032915131797E-4	0.001059146933763993	0.0	0.21442655024463475	2.7042049372697695E-4
70-71	1.8028032915131797E-4	0.0010704144543359504	0.0	0.2193391892140082	2.7042049372697695E-4
72-73	1.8028032915131797E-4	0.0010873157351938864	0.0	0.22315887868790174	3.267580965867638E-4
74-75	1.8028032915131797E-4	0.00111548453662378	0.0	0.2262405455643321	3.267580965867638E-4
76-77	1.8028032915131797E-4	0.001205624701199439	0.0	0.22897291930303174	3.267580965867638E-4
78-79	1.8028032915131797E-4	0.001239427262915311	0.0	0.2314686751097203	3.267580965867638E-4
80-81	1.8028032915131797E-4	0.001239427262915311	0.0	0.2327644399754954	3.323918568727425E-4
82-83	1.8591408943729665E-4	0.001239427262915311	0.0	0.23319260575722978	3.4929313773067857E-4
84-85	1.9154784972327533E-4	0.0012506947834872682	0.0	0.2335249976141025	3.549268980166572E-4
86-87	1.9718161000925401E-4	0.0012619623040592257	0.0	0.2336320390595361	3.6056065830263593E-4
88-89	2.028153702952327E-4	0.0013352011877769488	0.0	0.2337109117035398	3.6056065830263593E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10450	0.0	57.821617	1
GTATCAA	19375	0.0	41.7027	1
TCAACGC	24105	0.0	32.680847	4
ATCAACG	24150	0.0	32.62156	3
TATCAAC	25015	0.0	31.738472	2
CAACGCA	24890	0.0	29.862545	5
AACGCAG	25860	0.0	28.800774	6
ACGCAGA	29290	0.0	25.450737	7
CGCAGAG	30250	0.0	24.651941	8
GTGGTAT	4875	0.0	23.828344	1
GCAGAGT	34730	0.0	21.17448	9
TGGTATC	5000	0.0	21.171677	2
GTACATG	22560	0.0	20.118773	1
GAGTACT	21335	0.0	19.861574	12-13
TACATGG	22980	0.0	19.506474	2
AGAGTAC	31395	0.0	19.366941	10-11
CAGAGTA	33715	0.0	19.180912	10-11
ACATGGG	23445	0.0	18.473413	3
GTACTTT	23900	0.0	17.710155	14-15
AGTACTT	23530	0.0	17.52502	12-13
>>END_MODULE