FastQCFastQC Report
Fri 27 May 2016
SRR2138734_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138734_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21394447
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA267200.053.6497271
GTATCAA496200.040.474111
ATCAACG577100.033.8240433
TCAACGC585800.033.406784
TATCAAC614100.032.1351282
CAACGCA609300.030.6729555
CTTATAC194750.029.9212761
AACGCAG636200.029.5197436
ACGCAGA719400.026.0791387
CGCAGAG734300.025.5722928
TATACAC302400.022.5947253
GCAGAGT845500.021.9513139
GTGGTAT124000.021.5823841
TTATACA289400.021.2289562
GTACATG561750.020.7216281
TACATGG557200.020.4230692
GAGTACT520300.020.39663712-13
ACATCTC288500.019.5780758
CAGAGTA825950.019.34454310-11
ACATGGG572250.019.1817073
Produced by FastQC (version 0.11.5)