FastQCFastQC Report
Fri 27 May 2016
SRR2138733_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138733_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12921414
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA148750.055.8767851
GTATCAA276400.041.4006271
CTTATAC125550.034.755171
TCAACGC339350.033.014734
ATCAACG340250.032.9017453
TATCAAC351850.032.0322
CAACGCA352800.030.3043735
AACGCAG365650.029.3908086
TATACAC178800.027.3272423
ACGCAGA416600.025.6950447
TTATACA182000.025.3343662
CGCAGAG428800.025.0179028
GCAGAGT495450.021.4793789
GTACATG324100.021.2247681
TACATGG319300.020.9445882
ACATCTC199650.020.8231898
GTGGTAT73150.019.9051171
GAGTACT316400.019.72613712-13
ACACATC210450.019.6515796
ACATGGG331350.019.5304783
Produced by FastQC (version 0.11.5)