##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138733_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	12921414
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.766642799309736	33.0	31.0	34.0	30.0	34.0
2	31.463295735280983	33.0	31.0	34.0	28.0	34.0
3	31.788237726923693	33.0	31.0	34.0	30.0	34.0
4	32.96134618084368	37.0	35.0	37.0	26.0	37.0
5	34.414062114254676	37.0	35.0	37.0	28.0	37.0
6	35.094884275049154	37.0	35.0	37.0	32.0	37.0
7	35.23785709520645	37.0	35.0	37.0	32.0	37.0
8	35.38635469771342	37.0	35.0	37.0	32.0	37.0
9	37.135203391826934	39.0	37.0	39.0	33.0	39.0
10-11	37.01397281288255	39.0	37.0	39.0	33.0	39.0
12-13	36.90234946423046	39.0	37.0	39.0	32.0	39.0
14-15	38.247365884260034	40.0	38.0	41.0	32.0	41.0
16-17	38.239786760179655	40.0	38.0	41.0	32.0	41.0
18-19	38.17195765107441	40.0	38.0	41.0	32.0	41.0
20-21	38.23505093250873	40.0	38.0	41.0	32.0	41.0
22-23	38.280508804290434	40.0	38.0	41.0	32.0	41.0
24-25	38.16342264501954	40.0	38.0	41.0	32.0	41.0
26-27	38.07293977158054	40.0	38.0	41.0	32.0	41.0
28-29	38.082218240751565	40.0	38.0	41.0	32.0	41.0
30-31	38.032936999308895	40.0	37.0	41.0	32.0	41.0
32-33	37.926330800416295	40.0	37.0	41.0	31.5	41.0
34-35	37.81273122314683	40.0	37.0	41.0	31.0	41.0
36-37	37.76482332861833	40.0	37.0	41.0	31.0	41.0
38-39	37.69223137413909	40.0	37.0	41.0	31.0	41.0
40-41	37.61563316232944	40.0	37.0	41.0	31.0	41.0
42-43	37.589717552617515	40.0	37.0	41.0	31.0	41.0
44-45	37.5241516056201	40.0	36.0	41.0	30.5	41.0
46-47	37.38784255170682	40.0	36.0	41.0	30.0	41.0
48-49	37.56072853755043	40.0	36.0	41.0	31.0	41.0
50-51	37.506546229231645	40.0	36.0	41.0	30.0	41.0
52-53	37.44498186951926	40.0	36.0	41.0	30.0	41.0
54-55	37.315748026265965	39.0	36.0	41.0	30.0	41.0
56-57	37.179416323572525	39.0	35.0	41.0	30.0	41.0
58-59	37.07164748600532	39.0	35.0	41.0	30.5	41.0
60-61	36.859356384549955	39.0	35.0	41.0	30.0	41.0
62-63	36.6748176769468	38.5	35.0	40.5	30.0	41.0
64-65	36.41523962206118	38.0	35.0	40.0	30.0	41.0
66-67	36.19712723741114	37.5	34.5	40.0	30.0	41.0
68-69	35.942712098225996	37.0	34.0	40.0	30.0	41.0
70-71	35.69529147914438	36.5	34.0	39.0	30.0	41.0
72-73	35.39217532301248	36.0	34.0	39.0	30.0	41.0
74-75	35.21316888853282	36.0	34.0	39.0	30.0	40.0
76-77	35.02337432686669	35.0	34.0	37.5	30.0	40.0
78-79	34.77087668944452	35.0	34.0	37.0	30.0	39.0
80-81	34.5241391320385	35.0	34.0	37.0	30.0	39.0
82-83	34.28040645152892	35.0	34.0	36.0	30.0	38.5
84-85	34.07363861506444	35.0	34.0	36.0	30.0	37.0
86-87	33.8913287731583	35.0	34.0	36.0	29.0	37.0
88-89	33.75731355792569	35.0	34.0	35.0	29.0	36.5
90-91	33.62877396724032	35.0	34.0	35.0	29.0	36.0
92-93	33.58838649670744	35.0	34.0	35.0	29.0	36.0
94-95	33.5669578945304	35.0	34.0	35.0	29.0	36.0
96-97	33.57499524700847	35.0	34.0	35.0	30.0	36.0
98-99	33.6081292565147	35.0	34.0	35.0	30.0	35.0
100-101	33.22098319204905	34.5	33.0	35.0	29.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	12.0
17	122.0
18	689.0
19	2823.0
20	7405.0
21	16234.0
22	28532.0
23	45027.0
24	69809.0
25	104747.0
26	154843.0
27	216489.0
28	240444.0
29	203547.0
30	239396.0
31	294921.0
32	367509.0
33	479044.0
34	651853.0
35	921530.0
36	1409278.0
37	2440695.0
38	3217153.0
39	1723792.0
40	85520.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.26825547111175	20.403850538338915	14.628399028155897	24.699494962393434
2	14.249557329406958	20.760183235764853	41.77745184041767	23.212807594410524
3	17.599217123231266	25.99771279121821	30.723315845948378	25.679754239602147
4	12.129767120433858	18.366883741011023	38.48248012895855	31.020869009596574
5	13.220399505563341	37.51166671774484	35.55406500176607	13.713868774925759
6	29.668995084485694	38.10324407834958	18.68734206273159	13.54041877443313
7	26.057967025055866	32.73689961706656	23.0145488076796	18.19058455019797
8	23.997120112483707	37.09986535450122	21.15982880410682	17.74318572890826
9	25.129804267006193	16.979543513083513	21.219710467412995	36.67094175249729
10-11	24.00041806672317	26.805012391805054	28.9704326850867	20.224136856385076
12-13	24.893175630271934	24.298994427945235	28.928765950099926	21.879063991682905
14-15	22.015605576080066	25.644382225557944	26.536525782370298	25.803486415991696
16-17	21.68118593063838	28.877486268608816	27.151937486013995	22.289390314738814
18-19	21.7368286471665	27.82561293873855	28.979931448352257	21.457626965742683
20-21	22.434573141356136	26.994026462090243	28.893821900113565	21.67757849644006
22-23	22.12786094167283	27.150816452960164	28.72451656038151	21.9968060449855
24-25	21.995033211167193	27.28980047930028	28.852191075087237	21.86297523444529
26-27	21.997026178891304	27.446362074398916	28.81879192339303	21.737819823316755
28-29	21.850403553036088	27.25092629231895	28.959208867098546	21.93946128754642
30-31	22.132186137920872	27.410879569789298	28.66832221894621	21.78861207334362
32-33	22.255825853298873	27.249244346201824	28.66945569975475	21.825474100744554
34-35	22.11062286283018	27.241519528375203	28.769800016024995	21.878057592769622
36-37	21.95020266230862	27.42775942995934	28.693561791542482	21.928476116189557
38-39	21.871107250870256	27.233664544676046	28.897796894711064	21.99743130974263
40-41	22.110800940900994	27.12421187815004	28.78117237683676	21.983814804112207
42-43	22.16319957226147	27.137869191859536	28.605791872569135	22.09313936330986
44-45	22.053957951600182	27.39724265434413	28.405838146724925	22.142961247330764
46-47	22.33208370032453	27.185893840848706	28.04112449037941	22.440897968447352
48-49	22.20282155107936	27.338882113596476	27.9933221407959	22.464974194528263
50-51	22.05889054908823	27.480450918873274	28.051653793562824	22.409004738475673
52-53	22.213131401460657	27.456349985219035	28.103005623088883	22.227512990231418
54-55	22.21264189738063	27.384453357817666	27.981208736037516	22.42169600876418
56-57	22.374405352465427	27.56127693406027	27.751601365778072	22.312716347696234
58-59	22.09875308255578	27.830636087246752	27.979402770474344	22.091208059723122
60-61	22.347020857151115	27.93569951510696	27.63464719541451	22.082632432327415
62-63	22.17950345777617	28.199012585748672	27.55831083807589	22.063173118399266
64-65	22.165629752110252	28.07301665715659	27.580676953477457	22.1806766372557
66-67	22.23095091866422	28.007305643652785	27.504393318080755	22.257350119602233
68-69	22.19204424456	27.855271397230837	27.638150738279855	22.31453361992931
70-71	22.25548560686424	27.51307567305639	27.741685513368058	22.48975320671131
72-73	22.325373391940136	27.516303639676092	27.67577712090613	22.482545847477635
74-75	22.17914314727292	27.63278519531974	27.703308587990318	22.484763069417024
76-77	22.324170184904663	27.593059413899525	27.60992933657998	22.472841064615835
78-79	22.234634595754457	27.65174987151583	27.598281509410725	22.51533402331899
80-81	22.294718948222883	27.616410337759483	27.616621079414926	22.472249634602708
82-83	22.312368676562947	27.62574587643863	27.69501011259785	22.366875334400575
84-85	22.381417542477035	27.613913938757552	27.634996940125014	22.369671578640403
86-87	22.239356573057663	27.652563352240744	27.69214782811624	22.415932246585342
88-89	22.284752348101343	27.587949233267462	27.662268614511166	22.465029804120032
90-91	22.2765609737343	27.662534759986606	27.674203156722026	22.38670110955707
92-93	22.36835344568945	27.63056605894577	27.66464401014177	22.336436485223008
94-95	22.196024248925454	27.699972131856782	27.703779425778936	22.400224193438824
96-97	22.21297232758634	27.722943015068118	27.69703133839303	22.36705331895251
98-99	22.494823022281356	28.279366177538094	27.729041187264492	21.49676961291606
100-101	23.12980702917558	30.848183682140164	24.894697194770625	21.127312093913634
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3979.0
1	3243.0
2	3537.5
3	4675.5
4	5735.5
5	8795.5
6	11676.0
7	14429.0
8	16766.5
9	16733.5
10	14467.0
11	12057.5
12	12752.0
13	17199.0
14	28370.5
15	44083.0
16	57291.0
17	65135.5
18	67058.5
19	63837.5
20	58377.0
21	53528.5
22	52380.0
23	58157.0
24	70212.0
25	88806.5
26	112075.0
27	136258.5
28	162806.0
29	191824.0
30	217720.0
31	243416.5
32	271546.5
33	300132.5
34	328987.5
35	355133.5
36	383080.0
37	410200.0
38	432296.0
39	450802.0
40	468416.5
41	485482.5
42	498535.0
43	512775.5
44	534581.5
45	555348.0
46	558370.0
47	552786.5
48	544576.5
49	532380.0
50	520659.5
51	505958.5
52	484795.0
53	460354.0
54	435539.5
55	407346.0
56	375904.5
57	340522.5
58	304192.0
59	265375.0
60	222993.0
61	182000.0
62	143871.5
63	111084.0
64	84452.5
65	63753.0
66	48796.0
67	37377.5
68	28479.0
69	21524.5
70	15854.0
71	11547.0
72	8341.5
73	5846.5
74	4049.0
75	2781.0
76	1922.5
77	1308.5
78	956.0
79	704.0
80	482.0
81	328.5
82	246.5
83	183.5
84	135.5
85	101.0
86	76.5
87	57.0
88	37.5
89	24.5
90	16.0
91	12.0
92	10.0
93	5.5
94	2.0
95	1.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	0.0
2	1.7732734203857257
3	0.006671096522408461
4	8.651769845003031
5	3.250418259178136E-4
6	0.07462031632141808
7	0.0250901333244179
8	5.88170923089377E-4
9	2.78607279358126E-4
10-11	0.2964304061459528
12-13	0.3094862528203183
14-15	5.417363765296894E-5
16-17	1.3543409413242235E-4
18-19	0.48592205156494483
20-21	2.631712162519682E-4
22-23	1.1613523762605173E-4
24-25	0.17047305003127075
26-27	0.14816854703003116
28-29	1.4344120887598694E-4
30-31	1.3585353808609303E-4
32-33	8.947013877713225E-5
34-35	3.93991742947304E-4
36-37	2.858747895834276E-4
38-39	0.0014686339168915423
40-41	0.0018421821927696687
42-43	0.0012601028546992145
44-45	0.0010048593389858412
46-47	0.0014727441312769227
48-49	0.0024644262930214322
50-51	3.880327080200004E-4
52-53	0.0015766665334057573
54-55	5.995819949132805E-4
56-57	0.00896599001214088
58-59	2.0057429969803326E-4
60-61	0.002778469643043759
62-63	0.0015784116421903433
64-65	0.0015327736206234208
66-67	0.004754826748497635
68-69	0.0022050655250265504
70-71	0.0026311297825896097
72-73	0.0037797855598597245
74-75	7.189586473075996E-4
76-77	4.1237418721282003E-4
78-79	8.952365316755764E-4
80-81	4.1669199148166615E-4
82-83	0.0016176407697393463
84-85	2.88874474542227E-4
86-87	4.901005384323723E-4
88-89	1.1517713090962591E-4
90-91	2.2809585378508342E-4
92-93	0.0
94-95	0.0
96-97	5.281627189056215E-5
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	1301.0
20-21	3175.0
22-23	4191.0
24-25	5522.0
26-27	7391.0
28-29	12108.0
30-31	26571.0
32-33	33516.0
34-35	44819.0
36-37	64681.0
38-39	77539.0
40-41	80177.0
42-43	93327.0
44-45	114129.0
46-47	112358.0
48-49	104415.0
50-51	94541.0
52-53	92350.0
54-55	100200.0
56-57	105955.0
58-59	108349.0
60-61	108761.0
62-63	111985.0
64-65	120121.0
66-67	128111.0
68-69	130815.0
70-71	121396.0
72-73	107298.0
74-75	108760.0
76-77	112178.0
78-79	116745.0
80-81	116635.0
82-83	112170.0
84-85	111543.0
86-87	114228.0
88-89	119221.0
90-91	122948.0
92-93	126043.0
94-95	137276.0
96-97	213359.0
98-99	1056222.0
100-101	8238984.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.37751708464737
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.67645070207854	75.6667625120517
2	7.009401208463402	11.828717404405372
3	1.758725685611996	4.45190719754803
4	0.687191023794671	2.319338894026047
5	0.3347523702378346	1.4122786919434547
6	0.17652053136633322	0.8936618490692287
7	0.10556250043638096	0.6234971178848181
8	0.06691859843678427	0.4517140146304337
9	0.0442853253409285	0.33630172159980254
>10	0.13704269712216358	1.7510789478536453
>50	0.0025693909870569812	0.14231407500827323
>100	5.173537838130548E-4	0.07204260317241808
>500	3.130990460109185E-5	0.01845260347243726
>1k	3.13024355367749E-5	0.0319323673343007
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	7.739091093281278E-6	0.0	7.739091093281278E-6	0.0
3	0.0	7.739091093281278E-6	0.0	7.739091093281278E-6	0.0
4	0.0	2.321727327984383E-5	0.0	1.5478182186562555E-5	0.0
5	0.0	3.095636437312511E-5	0.0	1.5478182186562555E-5	0.0
6	0.0	3.095636437312511E-5	0.0	1.5478182186562555E-5	0.0
7	7.739091093281278E-6	3.8695455466406386E-5	0.0	2.321727327984383E-5	0.0
8	7.739091093281278E-6	4.643454655968766E-5	0.0	3.095636437312511E-5	0.0
9	7.739091093281278E-6	9.286909311937532E-5	0.0	3.095636437312511E-5	0.0
10-11	2.7086818826484472E-5	9.286909311937532E-5	0.0	5.4173637652968945E-5	0.0
12-13	3.8695455466406386E-5	9.286909311937532E-5	0.0	9.286909311937532E-5	0.0
14-15	3.8695455466406386E-5	1.5478182186562552E-4	0.0	1.2382545749250044E-4	0.0
16-17	3.8695455466406386E-5	1.625209129589068E-4	0.0	1.4704273077234425E-4	0.0
18-19	4.2565001013047024E-5	1.7799909514546939E-4	0.0	1.702600040521881E-4	0.0
20-21	4.643454655968766E-5	1.9734682287867258E-4	0.0	1.8186864069211E-4	0.0
22-23	5.417363765296894E-5	2.2830318725179769E-4	0.0	2.0508591397195384E-4	3.869545546640639E-6
24-25	6.578227429289085E-5	2.5539000607828217E-4	0.0	2.824768249047666E-4	7.739091093281278E-6
26-27	6.96518198395315E-5	2.631290971715634E-4	0.0	4.17910919037189E-4	7.739091093281278E-6
28-29	6.96518198395315E-5	2.708681882648447E-4	0.0	0.0012034286650052385	1.1608636639921917E-5
30-31	7.739091093281277E-5	3.250418259178136E-4	0.0	0.004314543284504312	1.5478182186562555E-5
32-33	7.739091093281277E-5	3.250418259178136E-4	0.0	0.012007199831225902	1.5478182186562555E-5
34-35	9.286909311937532E-5	4.101718279439077E-4	0.0	0.023120534641177817	1.5478182186562555E-5
36-37	1.0834727530593788E-4	4.720845566901579E-4	0.0	0.03890054138037834	1.5478182186562555E-5
38-39	1.0834727530593788E-4	4.875627388767205E-4	0.0	0.05959487096381247	1.5478182186562555E-5
40-41	1.0834727530593788E-4	5.494754676229706E-4	0.0	0.07884199051280301	1.5478182186562555E-5
42-43	1.0834727530593788E-4	5.804318319960958E-4	0.0	0.09577125227935582	1.5478182186562555E-5
44-45	1.0834727530593788E-4	6.500836518356272E-4	0.0	0.11403937680504625	1.5478182186562555E-5
46-47	1.0834727530593788E-4	7.042572894885962E-4	0.0	0.13096476902605242	1.5478182186562555E-5
48-49	1.0834727530593788E-4	7.081268350352368E-4	0.0	0.14497252390489151	1.5478182186562555E-5
50-51	1.0834727530593788E-4	8.551695658075812E-4	0.0	0.15622903190006915	1.5478182186562555E-5
52-53	1.1608636639921915E-4	8.66778202447503E-4	0.0	0.16498194392657026	1.5478182186562555E-5
54-55	1.1608636639921915E-4	8.66778202447503E-4	0.0	0.1732782495785678	1.5478182186562555E-5
56-57	1.1608636639921915E-4	8.745172935407843E-4	0.0	0.1798990420088699	1.5478182186562555E-5
58-59	1.1608636639921915E-4	8.78386839087425E-4	0.0	0.18541701395837948	1.5478182186562555E-5
60-61	1.1608636639921915E-4	9.016041123672688E-4	0.0	0.1907144218117305	1.5478182186562555E-5
62-63	1.1608636639921915E-4	9.286909311937532E-4	0.0	0.19574870056791	1.5478182186562555E-5
64-65	1.1608636639921915E-4	0.00102929911540641	0.0	0.20069784932206336	1.5478182186562555E-5
66-67	1.2382545749250044E-4	0.0010409077520463317	0.0	0.2057282585326962	1.5478182186562555E-5
68-69	1.315645485857817E-4	0.0010447772975929724	0.0	0.21065032046802307	1.5478182186562555E-5
70-71	1.315645485857817E-4	0.0010563859342328941	0.0	0.21555690422116341	1.5478182186562555E-5
72-73	1.315645485857817E-4	0.0010718641164194568	0.0	0.219383884766791	1.5478182186562555E-5
74-75	1.315645485857817E-4	0.0011182986629791444	0.0	0.2225453034783964	1.5478182186562555E-5
76-77	1.315645485857817E-4	0.0011647332095388323	0.0	0.22536620218189743	1.5478182186562555E-5
78-79	1.315645485857817E-4	0.001176341846178754	0.0	0.22785431996838734	1.5478182186562555E-5
80-81	1.315645485857817E-4	0.0011840809372720355	0.0	0.2290384009056594	1.5478182186562555E-5
82-83	1.315645485857817E-4	0.0011918200283653167	0.0	0.22954918091781595	1.5478182186562555E-5
84-85	1.315645485857817E-4	0.0012072982105518792	0.0	0.22991678774474683	1.5478182186562555E-5
86-87	1.315645485857817E-4	0.0012072982105518792	0.0	0.23004448274778597	1.5478182186562555E-5
88-89	1.315645485857817E-4	0.0013001673036712545	0.0	0.23010639547653222	1.5478182186562555E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	14875	0.0	55.876785	1
GTATCAA	27640	0.0	41.400627	1
CTTATAC	12555	0.0	34.75517	1
TCAACGC	33935	0.0	33.01473	4
ATCAACG	34025	0.0	32.901745	3
TATCAAC	35185	0.0	32.032	2
CAACGCA	35280	0.0	30.304373	5
AACGCAG	36565	0.0	29.390808	6
TATACAC	17880	0.0	27.327242	3
ACGCAGA	41660	0.0	25.695044	7
TTATACA	18200	0.0	25.334366	2
CGCAGAG	42880	0.0	25.017902	8
GCAGAGT	49545	0.0	21.479378	9
GTACATG	32410	0.0	21.224768	1
TACATGG	31930	0.0	20.944588	2
ACATCTC	19965	0.0	20.823189	8
GTGGTAT	7315	0.0	19.905117	1
GAGTACT	31640	0.0	19.726137	12-13
ACACATC	21045	0.0	19.651579	6
ACATGGG	33135	0.0	19.530478	3
>>END_MODULE