FastQCFastQC Report
Fri 27 May 2016
SRR2138732_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138732_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13379032
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA146200.055.7983971
GTATCAA274850.041.2591741
ATCAACG334400.033.115593
TCAACGC335200.033.076494
TATCAAC348400.032.1180532
CAACGCA348400.030.2502175
AACGCAG363300.029.1796366
ACGCAGA414650.025.4946547
CTTATAC184400.024.970511
CGCAGAG430250.024.554898
TATACAC236900.021.8234653
GTACATG341300.021.2614331
GCAGAGT495950.021.093549
TACATGG339600.020.773632
GAGTACT298300.020.0358612-13
TTATACA243950.019.8178962
ACATGGG347950.019.6947673
GTGGTAT75850.019.307161
AGAGTAC448500.018.9291210-11
CAGAGTA483350.018.62625910-11
Produced by FastQC (version 0.11.5)