##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138732_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	13379032
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.799214322829933	33.0	31.0	34.0	30.0	34.0
2	31.473461233966702	33.0	31.0	34.0	29.0	34.0
3	31.794902725398966	33.0	31.0	34.0	30.0	34.0
4	32.959743275896194	37.0	35.0	37.0	26.0	37.0
5	34.4174550894265	37.0	35.0	37.0	28.0	37.0
6	35.091468351372505	37.0	35.0	37.0	32.0	37.0
7	35.245325670795914	37.0	35.0	37.0	32.0	37.0
8	35.387861020139574	37.0	35.0	37.0	32.0	37.0
9	37.15524665760572	39.0	37.0	39.0	33.0	39.0
10-11	37.02360708906295	39.0	37.0	39.0	33.0	39.0
12-13	36.9170333474051	39.0	37.0	39.0	32.0	39.0
14-15	38.266726957525776	40.0	38.0	41.0	32.0	41.0
16-17	38.25635707426367	40.0	38.0	41.0	32.5	41.0
18-19	38.187646946356054	40.0	38.0	41.0	32.0	41.0
20-21	38.25126544268437	40.0	38.0	41.0	32.0	41.0
22-23	38.29147738832718	40.0	38.0	41.0	32.0	41.0
24-25	38.172607207653996	40.0	38.0	41.0	32.0	41.0
26-27	38.07753901216298	40.0	38.0	41.0	32.0	41.0
28-29	38.08101043586089	40.0	38.0	41.0	32.0	41.0
30-31	38.03487182306719	40.0	37.0	41.0	32.0	41.0
32-33	37.931697018186476	40.0	37.0	41.0	31.5	41.0
34-35	37.81787588277709	40.0	37.0	41.0	31.0	41.0
36-37	37.77199639905034	40.0	37.0	41.0	31.0	41.0
38-39	37.69655771492013	40.0	37.0	41.0	31.0	41.0
40-41	37.610415392945384	40.0	37.0	41.0	31.0	41.0
42-43	37.58631907197141	40.0	37.0	41.0	31.0	41.0
44-45	37.51957004984472	40.0	36.0	41.0	30.5	41.0
46-47	37.38349747648154	40.0	36.0	41.0	30.0	41.0
48-49	37.55906038668935	40.0	36.0	41.0	31.0	41.0
50-51	37.5031367937862	40.0	36.0	41.0	30.0	41.0
52-53	37.440644178281794	40.0	36.0	41.0	30.0	41.0
54-55	37.30940081581693	39.0	36.0	41.0	30.0	41.0
56-57	37.162518993903916	39.0	35.0	41.0	30.0	41.0
58-59	37.05083308012068	39.0	35.0	41.0	30.5	41.0
60-61	36.83229424820628	39.0	35.0	41.0	30.0	41.0
62-63	36.64529056969691	38.5	35.0	40.5	30.0	41.0
64-65	36.38889261319841	38.0	35.0	40.0	30.0	41.0
66-67	36.16414481340489	37.0	34.5	40.0	30.0	41.0
68-69	35.90966365512705	37.0	34.0	40.0	30.0	41.0
70-71	35.6585270017607	36.5	34.0	39.0	30.0	41.0
72-73	35.35408893133873	36.0	34.0	39.0	30.0	41.0
74-75	35.17989476854633	36.0	34.0	39.0	30.0	40.0
76-77	34.98781801700404	35.0	34.0	37.5	30.0	39.5
78-79	34.73553000869719	35.0	34.0	37.0	30.0	39.0
80-81	34.48755823432869	35.0	34.0	37.0	30.0	39.0
82-83	34.245637439052835	35.0	34.0	36.0	30.0	38.5
84-85	34.03911625492923	35.0	34.0	36.0	29.0	37.0
86-87	33.860381262777835	35.0	34.0	36.0	29.0	37.0
88-89	33.728190272261514	35.0	34.0	35.0	29.0	36.5
90-91	33.60195004758694	35.0	34.0	35.0	29.0	36.0
92-93	33.56382008146079	35.0	34.0	35.0	29.0	36.0
94-95	33.54533639697132	35.0	34.0	35.0	29.0	36.0
96-97	33.55262670264425	35.0	34.0	35.0	29.5	36.0
98-99	33.588254769556	35.0	34.0	35.0	30.0	35.0
100-101	33.19963825358974	34.5	33.0	35.0	29.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	0.0
16	10.0
17	101.0
18	704.0
19	2395.0
20	6792.0
21	14804.0
22	26883.0
23	43695.0
24	69120.0
25	106918.0
26	160171.0
27	226866.0
28	250698.0
29	210075.0
30	249975.0
31	305677.0
32	382053.0
33	498182.0
34	680269.0
35	963612.0
36	1477256.0
37	2552541.0
38	3327271.0
39	1738521.0
40	84442.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.20617485629753	20.34093348457497	14.631200523326351	24.821691135801156
2	14.243902372209453	20.701466133556707	41.69585155549928	23.35877993873456
3	17.618950730689498	25.872894875284285	30.727626301503058	25.78052809252316
4	12.15620912999995	18.2576346592332	38.31178152688274	31.274374683884105
5	13.228217720285615	37.42708246209745	35.55192498562858	13.79277483198835
6	29.858954276154424	38.033755409343314	18.572446491502205	13.534843823000047
7	26.138040363574817	32.67929032694101	22.981387634700255	18.20128167478392
8	24.17406242808197	37.09117765396634	20.99465675437686	17.74010316357483
9	25.167611593006562	16.886385429341228	21.11689687948955	36.82910609816267
10-11	24.02294419519995	26.798039619535814	28.851843820998997	20.32717236426524
12-13	24.821955997004245	24.291059993040292	28.877430325991003	22.00955368396446
14-15	21.99840870258061	25.704853569244985	26.420302084906073	25.87643564326834
16-17	21.73552599085015	28.951325717825586	27.06937117049565	22.243777120828618
18-19	21.76178054232617	27.90061173493519	28.77479986704614	21.562807855692494
20-21	22.462375582478998	27.048446531428667	28.74736081214716	21.741817073945178
22-23	22.17540530757255	27.228749546962227	28.584655047115554	22.01119009834967
24-25	22.078331564552364	27.370722541937383	28.656132457348725	21.89481343616153
26-27	22.009725459605594	27.553753825573164	28.586048222879356	21.850472491941886
28-29	21.882962333572216	27.318251326699126	28.692161210188775	22.10662512953988
30-31	22.3685845530996	27.24739097297058	28.61251936390543	21.771505110024393
32-33	22.240597436357135	27.30195100676604	28.64993372790462	21.807517828972205
34-35	22.210454522686966	27.396036818563392	28.716506025139992	21.67700263360965
36-37	22.21838774724221	27.231343463831436	28.55574424280356	21.994524546122793
38-39	21.971354694150453	27.2172390549056	28.745564458017437	22.065841792926516
40-41	22.118568608085713	27.05926733920775	28.742790327170663	22.079373725535866
42-43	22.124594724585727	27.135669272962915	28.60618706048307	22.13354894196829
44-45	22.021481969054214	27.3293942208376	28.42626242342035	22.22286138668784
46-47	22.337801728440162	27.13786197505893	27.998854001135992	22.52548229536491
48-49	22.224294212411106	27.30031017180714	28.04883206564191	22.426563550139843
50-51	22.111981583883292	27.491514439873576	28.01690088068029	22.379603095562846
52-53	22.21036381444771	27.551905365931074	28.034653850249292	22.20307696937192
54-55	22.16516545256543	27.496474556259514	27.895120189474344	22.443239801700717
56-57	22.320627376074547	27.700806004774353	27.633080176439673	22.345486442711426
58-59	22.081354083903545	27.85099365714505	27.942078656513054	22.125573602438347
60-61	22.31829891919092	27.96794064002927	27.579187320500637	22.13457312027917
62-63	22.11671454054765	28.20351647625084	27.56350941705174	22.116259566149772
64-65	22.107970690153657	28.072038059305825	27.563174384819433	22.256816865721085
66-67	22.195871640993058	28.074967581075423	27.401522032516212	22.327638745415307
68-69	22.17437830524642	27.892850915251294	27.525732935055373	22.40703784444691
70-71	22.238916377781603	27.572258892561518	27.604946004595458	22.583878725061425
72-73	22.289533314101874	27.565006375009084	27.571828405454983	22.573631905434063
74-75	22.197940513405822	27.636953848337807	27.57894784591955	22.586157792336824
76-77	22.37675775729423	27.614033092206324	27.47746363643388	22.531745514065562
78-79	22.256568708801904	27.657363526256106	27.473272815812805	22.612794949129185
80-81	22.293805328584636	27.646871929855894	27.470834075680532	22.58848866587894
82-83	22.391697114528583	27.624465209043546	27.58528016148584	22.398557514942027
84-85	22.447982486135224	27.644199083660435	27.518022788502222	22.38979564170212
86-87	22.29152267515734	27.690460493506492	27.540285435576944	22.477731395759225
88-89	22.338073478115753	27.609081902248878	27.528749440955764	22.52409517867961
90-91	22.378856924200786	27.684584235459692	27.533196079921286	22.403362760418236
92-93	22.43981749850702	27.65694958163539	27.5738158947752	22.329417025082392
94-95	22.282425134035744	27.729138245322616	27.588712606277028	22.399724014364615
96-97	22.26545657071973	27.71002253107516	27.656580846054528	22.36794005215058
98-99	22.533093560147847	28.259682182904257	27.703560728281428	21.503663528666475
100-101	23.223260911626177	30.856153421493353	24.83836385307146	21.08222181380901
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3976.0
1	3220.0
2	3463.0
3	4555.5
4	5552.5
5	8555.0
6	11398.0
7	14002.0
8	15941.5
9	15762.0
10	13765.5
11	11523.0
12	12225.5
13	16536.5
14	27918.5
15	44026.0
16	57119.5
17	64810.5
18	66520.5
19	63069.0
20	57591.5
21	52779.5
22	51450.5
23	56774.5
24	68436.0
25	86068.0
26	109010.5
27	134045.5
28	161698.0
29	190655.5
30	219062.0
31	248441.0
32	279307.5
33	310227.5
34	339245.5
35	367168.5
36	397208.0
37	424189.0
38	445661.5
39	464761.0
40	482882.5
41	501376.5
42	514695.5
43	526747.0
44	541128.0
45	556708.5
46	576723.0
47	588314.5
48	587798.0
49	577151.0
50	555621.5
51	532746.5
52	504769.0
53	474362.0
54	446318.5
55	415080.0
56	381265.0
57	346166.0
58	308813.5
59	268199.5
60	224993.0
61	183891.5
62	147013.5
63	114976.5
64	88251.5
65	66891.0
66	51250.0
67	38662.5
68	28571.5
69	21717.0
70	16322.5
71	11815.5
72	8479.0
73	5942.5
74	4118.0
75	2806.5
76	1909.5
77	1280.5
78	896.0
79	693.0
80	520.0
81	372.5
82	293.0
83	209.5
84	146.5
85	104.5
86	74.0
87	52.0
88	31.5
89	17.5
90	8.5
91	5.0
92	5.5
93	5.0
94	4.0
95	4.0
96	4.0
97	3.5
98	1.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	0.0
2	1.775375079452684
3	0.006323327427574731
4	8.673362915941901
5	3.662447328027917E-4
6	0.07499795201924923
7	0.02547269488554927
8	6.353224956783122E-4
9	2.6160338057342267E-4
10-11	0.29355262772373963
12-13	0.30515286905659544
14-15	8.969258762517348E-5
16-17	1.1959011683356464E-4
18-19	0.47188765226064183
20-21	2.653804808544803E-4
22-23	1.9067367214200447E-4
24-25	0.1730912372322726
26-27	0.15261540211490643
28-29	1.2355390450930566E-4
30-31	1.3869160736600873E-4
32-33	1.164413276565056E-4
34-35	3.803711752313137E-4
36-37	2.9482951804407674E-4
38-39	0.0016375773156863038
40-41	0.001933342477864948
42-43	0.0012994788090376675
44-45	0.001053641835530848
46-47	0.0015553828047194223
48-49	0.0026014165185928558
50-51	3.4166191187172645E-4
52-53	0.0017166407876732228
54-55	5.5608919074234E-4
56-57	0.008812190971792583
58-59	1.9660362325732393E-4
60-61	0.002824435060262403
62-63	0.0015819501739122558
64-65	0.0017237318127855301
66-67	0.004966310441084183
68-69	0.002140847521473627
70-71	0.0026671972006632257
72-73	0.003809445796100404
74-75	7.827168993025814E-4
76-77	4.35748149249581E-4
78-79	7.85503224695059E-4
80-81	4.2692910901823014E-4
82-83	0.0017173914476319538
84-85	3.0501075420994616E-4
86-87	4.4128467744296496E-4
88-89	1.1519249866448696E-4
90-91	2.6235362489630956E-4
92-93	0.0
94-95	0.0
96-97	4.556844585625616E-5
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	1288.0
20-21	3152.0
22-23	4304.0
24-25	5612.0
26-27	7499.0
28-29	12181.0
30-31	26080.0
32-33	32605.0
34-35	43374.0
36-37	63993.0
38-39	73744.0
40-41	78586.0
42-43	91783.0
44-45	111665.0
46-47	111579.0
48-49	102595.0
50-51	92673.0
52-53	85494.0
54-55	93377.0
56-57	102360.0
58-59	115102.0
60-61	113981.0
62-63	112395.0
64-65	122048.0
66-67	130269.0
68-69	135421.0
70-71	125070.0
72-73	110303.0
74-75	111873.0
76-77	115945.0
78-79	120778.0
80-81	120427.0
82-83	117120.0
84-85	117187.0
86-87	119179.0
88-89	124337.0
90-91	128892.0
92-93	130713.0
94-95	144673.0
96-97	225557.0
98-99	1109518.0
100-101	8584300.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.5007034834809
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.53041236996229	75.65382828427956
2	7.208053225050463	12.18171136526275
3	1.7972766796160715	4.556134313460384
4	0.6661468405809313	2.251595066095476
5	0.309191369738119	1.3063444126946053
6	0.15586337339187142	0.7902338819352958
7	0.09515978427014728	0.5628748099914607
8	0.06641912770462317	0.4489970412639852
9	0.03809562656640806	0.2897196520054919
>10	0.1300925255127274	1.6976309981425672
>50	0.002613304897887194	0.14697501963717932
>100	6.457428984487427E-4	0.09110478428512539
>500	1.5015524400219332E-5	0.009275091561190834
>1k	1.5014285647662745E-5	0.013575279384964929
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.242314690629337E-5	0.0	7.47438230209779E-6	0.0
3	0.0	2.242314690629337E-5	0.0	7.47438230209779E-6	0.0
4	0.0	8.969258762517348E-5	0.0	1.494876460419558E-5	0.0
5	0.0	9.716696992727127E-5	0.0	2.989752920839116E-5	0.0
6	0.0	9.716696992727127E-5	0.0	5.232067611468453E-5	7.47438230209779E-6
7	0.0	9.716696992727127E-5	0.0	5.979505841678232E-5	7.47438230209779E-6
8	0.0	1.1959011683356464E-4	0.0	7.47438230209779E-5	7.47438230209779E-6
9	0.0	2.466546159692271E-4	0.0	1.0464135222936906E-4	2.242314690629337E-5
10-11	0.0	2.466546159692271E-4	0.0	1.3827607258880912E-4	2.242314690629337E-5
12-13	0.0	2.466546159692271E-4	0.0	1.5322483719300468E-4	2.242314690629337E-5
14-15	0.0	3.4008439474544943E-4	0.0	1.8685955755244475E-4	2.242314690629337E-5
16-17	0.0	3.587703505006939E-4	0.0	2.1675708676083591E-4	2.242314690629337E-5
18-19	0.0	3.774563062559384E-4	0.0	2.541289982713249E-4	2.242314690629337E-5
20-21	0.0	3.9987945316223176E-4	0.0	2.6907776287552046E-4	2.242314690629337E-5
22-23	0.0	4.409885558237696E-4	0.0	3.2139843899020495E-4	2.242314690629337E-5
24-25	0.0	4.746232761832097E-4	0.0	3.9987945316223176E-4	2.242314690629337E-5
26-27	0.0	5.045208053916009E-4	0.0	5.344183345999921E-4	2.242314690629337E-5
28-29	0.0	5.082579965426497E-4	0.0	0.0019470765896964742	2.242314690629337E-5
30-31	0.0	5.755274372615299E-4	0.0	0.006794213512606891	2.242314690629337E-5
32-33	0.0	5.755274372615299E-4	0.0	0.01906714925265146	2.242314690629337E-5
34-35	0.0	6.951175540950945E-4	0.0	0.03492031411540088	2.242314690629337E-5
36-37	0.0	8.147076709286591E-4	0.0	0.057313563492485856	2.242314690629337E-5
38-39	0.0	8.520795824391481E-4	0.0	0.09263375706104895	2.242314690629337E-5
40-41	0.0	9.903556550279573E-4	0.0	0.14573176893515166	2.989752920839116E-5
42-43	3.737191151048895E-6	0.0010277275665384461	0.0	0.17516588644081277	3.363472035944006E-5
44-45	7.47438230209779E-6	0.0011622664479762064	0.0	0.21145774970864858	3.737191151048895E-5
46-47	7.47438230209779E-6	0.0012556962267524286	0.0	0.248788552116476	3.737191151048895E-5
48-49	7.47438230209779E-6	0.0012669078002055753	0.0	0.2790261657196126	3.737191151048895E-5
50-51	7.47438230209779E-6	0.0013416516232265534	0.0	0.3043867448706304	3.737191151048895E-5
52-53	7.47438230209779E-6	0.0013528631966797	0.0	0.33721796913259494	4.110910266153784E-5
54-55	7.47438230209779E-6	0.0013752863435859933	0.0	0.40233478774847087	4.484629381258674E-5
56-57	7.47438230209779E-6	0.0013939722993412378	0.0	0.4698097739806587	4.484629381258674E-5
58-59	7.47438230209779E-6	0.0014051838727943845	0.0	0.510724542702342	4.484629381258674E-5
60-61	7.47438230209779E-6	0.0014350814020027756	0.0	0.5298739101603165	4.484629381258674E-5
62-63	7.47438230209779E-6	0.0014874020781174603	0.0	0.5419973582543192	5.232067611468453E-5
64-65	7.47438230209779E-6	0.0016518384887636117	0.0	0.551616888277119	5.232067611468453E-5
66-67	7.47438230209779E-6	0.001666787253367807	0.0	0.5581793959383609	5.232067611468453E-5
68-69	7.47438230209779E-6	0.0017041591648782961	0.0	0.5642635431322685	5.232067611468453E-5
70-71	7.47438230209779E-6	0.0017490054586908827	0.0	0.5700599266075452	5.232067611468453E-5
72-73	7.47438230209779E-6	0.001782640179050323	0.0	0.5742530550790221	5.979505841678232E-5
74-75	7.47438230209779E-6	0.0017975889436545184	0.0	0.5771867501325956	5.979505841678232E-5
76-77	7.47438230209779E-6	0.0018723327666754965	0.0	0.580198926200341	6.726944071888011E-5
78-79	7.47438230209779E-6	0.0019059674870349364	0.0	0.5827775880945647	7.47438230209779E-5
80-81	7.47438230209779E-6	0.0019134418693370343	0.0	0.58393611735139	7.47438230209779E-5
82-83	7.47438230209779E-6	0.0019209162516391322	0.0	0.5843247852310989	9.716696992727127E-5
84-85	7.47438230209779E-6	0.0019433393985454255	0.0	0.5846611324346933	9.716696992727127E-5
86-87	7.47438230209779E-6	0.001969499736602768	0.0	0.5847956713161311	9.716696992727127E-5
88-89	7.47438230209779E-6	0.0020479807507747944	0.0	0.5848405176099437	9.716696992727127E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	14620	0.0	55.798397	1
GTATCAA	27485	0.0	41.259174	1
ATCAACG	33440	0.0	33.11559	3
TCAACGC	33520	0.0	33.07649	4
TATCAAC	34840	0.0	32.118053	2
CAACGCA	34840	0.0	30.250217	5
AACGCAG	36330	0.0	29.179636	6
ACGCAGA	41465	0.0	25.494654	7
CTTATAC	18440	0.0	24.97051	1
CGCAGAG	43025	0.0	24.55489	8
TATACAC	23690	0.0	21.823465	3
GTACATG	34130	0.0	21.261433	1
GCAGAGT	49595	0.0	21.09354	9
TACATGG	33960	0.0	20.77363	2
GAGTACT	29830	0.0	20.03586	12-13
TTATACA	24395	0.0	19.817896	2
ACATGGG	34795	0.0	19.694767	3
GTGGTAT	7585	0.0	19.30716	1
AGAGTAC	44850	0.0	18.92912	10-11
CAGAGTA	48335	0.0	18.626259	10-11
>>END_MODULE