FastQCFastQC Report
Fri 27 May 2016
SRR2138731_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138731_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9512069
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA108150.054.2166821
GTATCAA197850.041.296041
ATCAACG239200.033.2920343
TCAACGC239950.033.2246784
TATCAAC254300.031.7410772
CAACGCA250350.030.3027735
AACGCAG259250.029.3956016
CTTATAC112450.027.3251881
ACGCAGA298150.025.597547
CGCAGAG305400.024.9431448
TATACAC154700.022.8348753
GTACATG231800.021.8904761
GTGGTAT54500.021.1209981
GCAGAGT358300.021.0198579
TTATACA156300.020.8915522
TACATGG237050.020.8903432
ACATGGG242150.019.7859753
GAGTACT224700.019.51420612-13
AGAGTAC322300.019.09895510-11
TGGTATC52750.019.0605832
Produced by FastQC (version 0.11.5)