##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138731_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9512069
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.85740526062206	33.0	31.0	34.0	30.0	34.0
2	31.51680291637918	33.0	31.0	34.0	30.0	34.0
3	31.833271814996294	33.0	31.0	34.0	30.0	34.0
4	32.96330714169546	37.0	35.0	37.0	26.0	37.0
5	34.435915256712285	37.0	35.0	37.0	28.0	37.0
6	35.182366107731134	37.0	35.0	37.0	32.0	37.0
7	35.31386935902168	37.0	35.0	37.0	32.0	37.0
8	35.44682402955656	37.0	35.0	37.0	32.0	37.0
9	37.199774833424776	39.0	37.0	39.0	33.0	39.0
10-11	37.07012722468687	39.0	37.0	39.0	33.0	39.0
12-13	36.969423108684346	39.0	37.0	39.0	32.5	39.0
14-15	38.33001647696206	40.0	38.0	41.0	33.0	41.0
16-17	38.324004588276225	40.0	38.0	41.0	33.0	41.0
18-19	38.251069667387824	40.0	38.0	41.0	33.0	41.0
20-21	38.31588505869626	40.0	38.0	41.0	32.5	41.0
22-23	38.3621631096837	40.0	38.0	41.0	33.0	41.0
24-25	38.24209278692619	40.0	38.0	41.0	32.0	41.0
26-27	38.14343097804942	40.0	38.0	41.0	32.0	41.0
28-29	38.15530789114956	40.0	38.0	41.0	32.0	41.0
30-31	38.11054726051047	40.0	37.5	41.0	32.0	41.0
32-33	38.01071770733433	40.0	37.0	41.0	32.0	41.0
34-35	37.90096856445052	40.0	37.0	41.0	31.0	41.0
36-37	37.85816150731384	40.0	37.0	41.0	31.0	41.0
38-39	37.789260283266735	40.0	37.0	41.0	31.0	41.0
40-41	37.700612340251524	40.0	37.0	41.0	31.0	41.0
42-43	37.67445634170313	40.0	37.0	41.0	31.0	41.0
44-45	37.60771193224635	40.0	37.0	41.0	31.0	41.0
46-47	37.47404984275535	40.0	36.0	41.0	30.0	41.0
48-49	37.650357487770805	40.0	37.0	41.0	31.0	41.0
50-51	37.60385931547604	40.0	36.5	41.0	31.0	41.0
52-53	37.54486737680459	40.0	36.0	41.0	31.0	41.0
54-55	37.41307705296663	39.5	36.0	41.0	30.0	41.0
56-57	37.27562911959484	39.0	35.5	41.0	30.0	41.0
58-59	37.16901788348533	39.0	35.0	41.0	30.5	41.0
60-61	36.9479245169559	39.0	35.0	41.0	30.5	41.0
62-63	36.76342749938178	38.5	35.0	41.0	30.0	41.0
64-65	36.502981610207435	38.0	35.0	40.0	30.0	41.0
66-67	36.280464173226946	37.5	35.0	40.0	30.0	41.0
68-69	36.024905278295556	37.0	34.0	40.0	30.0	41.0
70-71	35.774276598843684	36.5	34.0	39.0	30.0	41.0
72-73	35.46655330402261	36.0	34.0	39.0	30.0	41.0
74-75	35.2855109619486	36.0	34.0	39.0	30.0	40.5
76-77	35.08550162246752	35.0	34.0	37.5	30.0	40.0
78-79	34.826129846617214	35.0	34.0	37.0	30.0	39.0
80-81	34.57006827439638	35.0	34.0	37.0	30.0	39.0
82-83	34.32172848439504	35.0	34.0	36.0	30.0	38.5
84-85	34.11045519197028	35.0	34.0	36.0	30.0	37.0
86-87	33.92344147194448	35.0	34.0	36.0	29.5	37.0
88-89	33.78795977916161	35.0	34.0	35.0	29.0	36.5
90-91	33.6561994984403	35.0	34.0	35.0	29.0	36.0
92-93	33.615465066674986	35.0	34.0	35.0	29.0	36.0
94-95	33.59295036523063	35.0	34.0	35.0	29.5	36.0
96-97	33.59711176751224	35.0	34.0	35.0	30.0	36.0
98-99	33.628330733275305	35.0	34.0	35.0	30.0	35.0
100-101	33.24302732392626	34.5	33.0	35.0	29.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	2.0
16	6.0
17	67.0
18	366.0
19	1410.0
20	3859.0
21	8782.0
22	16221.0
23	27272.0
24	43974.0
25	70142.0
26	108210.0
27	156953.0
28	179511.0
29	144922.0
30	169427.0
31	210891.0
32	263275.0
33	347333.0
34	475975.0
35	675269.0
36	1035531.0
37	1797859.0
38	2425146.0
39	1287512.0
40	62154.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.25321935742897	20.372875764463018	14.672601723137205	24.701303154970805
2	14.09325498375688	20.745288210944242	42.02415802401455	23.13729878128433
3	17.590686708969223	25.94415885978652	30.790659121259967	25.67449530998429
4	12.000355233321715	18.39295394015263	38.6044859379899	31.002204888535758
5	13.069461357225586	37.556266864815605	35.695046182011275	13.679225595947534
6	29.645682893891294	38.264208518272405	18.67620936162405	13.413899226212244
7	26.069781256496704	32.82805610438512	22.995908043119652	18.106254595998518
8	24.01818879118319	37.306765382549976	21.102580610705708	17.572465215561124
9	25.08517882638721	17.041263568188842	21.19185281569782	36.68170478972612
10-11	23.86161820731815	26.890475549281927	29.04011170216636	20.207794541233564
12-13	24.74730173175843	24.391975810693207	29.064947466189096	21.795774991359266
14-15	21.910012131967207	25.82313925690124	26.59052823468313	25.676320376448423
16-17	21.556904042942552	29.13847131067444	27.243192508203013	22.061432138179995
18-19	21.580513714290063	28.00392334941244	29.07349935635685	21.34206357994065
20-21	22.207448996666905	27.114284360940527	29.076390800818587	21.601875841573985
22-23	21.902995365595295	27.27356238673288	28.93924531268363	21.88419693498819
24-25	21.766134071635417	27.3723481012895	29.080076524561992	21.781441302513088
26-27	21.722634719378593	27.482304156617783	29.049471828647437	21.745589295356186
28-29	21.49493733294947	27.295280368999634	29.095271902613817	22.11451039543708
30-31	21.773689015778192	27.152243143647542	29.143496686383365	21.9305711541909
32-33	21.624189189624563	27.377724384392028	29.07920018299664	21.918886242986773
34-35	21.7630131491682	27.558813834624686	29.12585897176232	21.5523140444448
36-37	21.7967865989745	27.428852968079624	28.818950873436012	21.955409559509864
38-39	21.740473163039876	27.37773406074619	29.018456262025015	21.86333651418892
40-41	21.951036100789644	27.209416335268788	28.948213840756814	21.891333723184754
42-43	21.96340136597818	27.280904846913174	28.82453055680746	21.931163230301188
44-45	21.88500877163226	27.489172197386036	28.635122694768267	21.990696336213432
46-47	22.156277753291285	27.375187365614195	28.219464183780236	22.249070697314284
48-49	22.008855224338628	27.527478005962568	28.211976987273868	22.251689782424933
50-51	21.908351620327828	27.685173544266288	28.229239078661507	22.17723575674438
52-53	22.025585392397034	27.670878642181364	28.27845102134755	22.025084944074056
54-55	21.943467576159385	27.651399356503394	28.123176807754398	22.28195625958282
56-57	22.1389389132941	27.8264340754451	27.92067936985918	22.113947641401623
58-59	21.893329033912106	28.045135403851557	28.12315355179144	21.938382010444897
60-61	22.141767869798617	28.13114464185923	27.81525503990987	21.911832448432282
62-63	21.939596907790936	28.382783757366088	27.741880183934096	21.935739150908883
64-65	21.913548809787674	28.27947211099672	27.748020209092434	22.05895887012317
66-67	21.97993243991609	28.19879964949708	27.692003332791902	22.129264577794928
68-69	21.99193326500192	28.051381594661684	27.794001953189955	22.16268318714644
70-71	22.03899728126927	27.72732863528476	27.879653642108952	22.354020441337013
72-73	22.102367104759445	27.71063263252823	27.857490798272277	22.329509464440044
74-75	21.99884034609961	27.80741511024666	27.843402433277014	22.35034211037672
76-77	22.10729535864447	27.759336700816085	27.778740255698537	22.35462768484091
78-79	22.059484857906718	27.783882895814727	27.800689477272027	22.355942769006525
80-81	22.04973554595566	27.847408880618485	27.76007157386241	22.342783999563444
82-83	22.091352407265536	27.81578936583513	27.83810864682512	22.25474958007421
84-85	22.18495860722989	27.78952041536829	27.77791548812001	22.247605489281813
86-87	22.020339615736358	27.807651094957752	27.85821997155567	22.313789317750214
88-89	22.078899800081356	27.80637167658529	27.82583314056736	22.28889538276599
90-91	22.08692864795535	27.820352422410018	27.860740718770177	22.231978210864458
92-93	22.086763818162332	27.760650035031514	27.892490031240452	22.260096115565702
94-95	21.987636396924714	27.830812692208827	27.910305756294207	22.27124515457225
96-97	22.019000324220933	27.840065929409374	27.890976478787827	22.249957267581866
98-99	22.267278495778157	28.411134562752466	27.96900308194	21.352583859529382
100-101	22.937804228376848	30.989855275292733	25.12563055872813	20.946709937602293
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2993.0
1	2379.0
2	2437.0
3	3218.5
4	4019.0
5	6195.5
6	8211.5
7	10093.0
8	11670.5
9	11752.5
10	10353.0
11	8723.5
12	9211.0
13	12372.5
14	20526.5
15	31711.0
16	40921.5
17	46704.0
18	48455.0
19	46205.5
20	42125.0
21	38617.5
22	37870.0
23	41984.5
24	50580.0
25	63560.5
26	80258.0
27	98373.0
28	118357.5
29	140252.0
30	161431.5
31	183393.0
32	206290.0
33	228205.5
34	248787.0
35	268063.5
36	290465.0
37	311569.0
38	327621.5
39	341717.0
40	355123.5
41	368578.5
42	378310.0
43	386696.0
44	400597.0
45	416513.0
46	418015.0
47	411821.5
48	403402.0
49	389483.5
50	378710.0
51	366153.0
52	347397.0
53	326976.5
54	307075.0
55	283986.5
56	259141.5
57	232582.0
58	205451.0
59	178952.5
60	150220.5
61	122180.0
62	97001.0
63	74951.0
64	56542.0
65	41888.5
66	30955.5
67	23555.5
68	17903.5
69	12991.0
70	9381.5
71	6782.5
72	4798.5
73	3235.5
74	2201.0
75	1524.0
76	1068.0
77	732.0
78	488.5
79	335.0
80	239.5
81	187.0
82	147.0
83	109.0
84	82.5
85	60.0
86	47.0
87	35.0
88	19.0
89	7.5
90	5.5
91	5.0
92	3.5
93	1.5
94	1.0
95	1.0
96	1.0
97	1.0
98	1.0
99	1.0
100	0.5
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	0.0
2	1.8158930512383795
3	0.0061711074635812675
4	8.730498065142294
5	3.364147169243621E-4
6	0.07503099483403664
7	0.025630596245674837
8	5.9923871452152E-4
9	2.312851178854989E-4
10-11	0.2972854801620972
12-13	0.30872883701747744
14-15	9.461663913497684E-5
16-17	1.261555188466358E-4
18-19	0.4961801685837224
20-21	2.838862535073752E-4
22-23	1.8404024066153528E-4
24-25	0.17699711791884634
26-27	0.16648695785510453
28-29	1.2636380772061806E-4
30-31	1.2123390817532955E-4
32-33	1.3203965457158782E-4
34-35	4.236097854919473E-4
36-37	3.0823181966355324E-4
38-39	0.0013993717141470286
40-41	0.0018960449576430002
42-43	0.00114034686649435
44-45	0.0010073159451362374
46-47	0.0013897051194045075
48-49	0.002409564475375497
50-51	3.517075204725877E-4
52-53	0.0017206129430578326
54-55	4.870317048578808E-4
56-57	0.010485187945424284
58-59	1.1512182104761893E-4
60-61	0.002550383434754162
62-63	0.0013073989930213622
64-65	0.0015032602103771237
66-67	0.00481404020506974
68-69	0.0020317600375295104
70-71	0.0025014803993414687
72-73	0.003496942029227268
74-75	7.236088168241046E-4
76-77	2.267942829697149E-4
78-79	6.111446816279977E-4
80-81	4.1828037088727433E-4
82-83	0.0015091795521002282
84-85	2.367005054147542E-4
86-87	3.256922822095709E-4
88-89	1.2772619805493168E-4
90-91	2.0408395115781248E-4
92-93	0.0
94-95	0.0
96-97	4.958587295645992E-5
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	762.0
20-21	1917.0
22-23	2697.0
24-25	3691.0
26-27	4818.0
28-29	8266.0
30-31	17839.0
32-33	22503.0
34-35	30736.0
36-37	44139.0
38-39	52395.0
40-41	55735.0
42-43	64711.0
44-45	79013.0
46-47	77832.0
48-49	72127.0
50-51	65500.0
52-53	61892.0
54-55	67555.0
56-57	72326.0
58-59	78548.0
60-61	79551.0
62-63	78480.0
64-65	85119.0
66-67	91365.0
68-69	94619.0
70-71	85991.0
72-73	77244.0
74-75	78085.0
76-77	81096.0
78-79	84578.0
80-81	84089.0
82-83	82079.0
84-85	81594.0
86-87	83827.0
88-89	87086.0
90-91	90476.0
92-93	91753.0
94-95	101006.0
96-97	157473.0
98-99	785097.0
100-101	6146459.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.25347978982212
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.61499015147308	78.15858221685014
2	6.624526182998518	11.427768704848203
3	1.5401099794812028	3.9851953496785586
4	0.5657012077761538	1.9517479076799376
5	0.24497581455434145	1.056500823482905
6	0.1371673910312805	0.7098698874083509
7	0.07822635359965659	0.47231066464676214
8	0.045501926373879856	0.3139759589509936
9	0.03229635394113178	0.2507105620751716
>10	0.11419733307226937	1.501228884178542
>50	0.001993983552296299	0.11171630745145048
>100	2.7471542044475093E-4	0.04004690592969247
>500	3.860672567110213E-5	0.020345826819293793
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	1.0512959903886316E-5	0.0
3	0.0	0.0	0.0	1.0512959903886316E-5	0.0
4	0.0	2.102591980777263E-5	0.0	1.0512959903886316E-5	0.0
5	0.0	2.102591980777263E-5	0.0	1.0512959903886316E-5	0.0
6	9.461663913497684E-5	2.102591980777263E-5	0.0	1.0512959903886316E-5	4.205183961554526E-5
7	9.461663913497684E-5	2.102591980777263E-5	0.0	1.0512959903886316E-5	5.256479951943158E-5
8	9.461663913497684E-5	3.153887971165895E-5	0.0	1.0512959903886316E-5	5.256479951943158E-5
9	9.461663913497684E-5	6.30777594233179E-5	0.0	3.153887971165895E-5	6.30777594233179E-5
10-11	9.461663913497684E-5	6.30777594233179E-5	0.0	6.30777594233179E-5	6.30777594233179E-5
12-13	9.461663913497684E-5	6.30777594233179E-5	0.0	6.833423937526105E-5	6.30777594233179E-5
14-15	9.461663913497684E-5	9.461663913497684E-5	0.0	8.936015918303368E-5	1.1564255894274948E-4
16-17	2.102591980777263E-4	9.461663913497684E-5	0.0	9.461663913497684E-5	1.3141199879857895E-4
18-19	2.102591980777263E-4	9.987311908692E-5	0.0	9.461663913497684E-5	1.366684787505221E-4
20-21	2.102591980777263E-4	1.1564255894274947E-4	0.0	9.461663913497684E-5	1.366684787505221E-4
22-23	2.1551567802966946E-4	1.629508785102379E-4	0.0	1.1038607899080631E-4	1.5243791860635158E-4
24-25	2.2077215798161262E-4	2.102591980777263E-4	0.0	1.629508785102379E-4	1.7872031836606736E-4
26-27	2.2077215798161262E-4	2.2077215798161262E-4	0.0	3.469276768282484E-4	1.8397679831801052E-4
28-29	2.2077215798161262E-4	2.4179807778938528E-4	0.0	0.0012457857486105283	1.8923327826995368E-4
30-31	2.2077215798161262E-4	2.8384991740493053E-4	0.0	0.0052091716323756695	1.8923327826995368E-4
32-33	2.2077215798161262E-4	2.8910639735687366E-4	0.0	0.014870581784047193	1.9974623817384E-4
34-35	2.2602863793355578E-4	3.732100765879642E-4	0.0	0.028374478780589164	2.102591980777263E-4
36-37	2.3128511788549893E-4	4.5205727586711156E-4	0.0	0.04739767972667146	2.102591980777263E-4
38-39	2.3128511788549893E-4	4.6257023577099787E-4	0.0	0.07581421034687616	2.102591980777263E-4
40-41	2.3128511788549893E-4	5.309044751462589E-4	0.0	0.11184738041744652	2.2077215798161262E-4
42-43	2.3128511788549893E-4	5.414174350501452E-4	0.0	0.13492332740647697	2.2602863793355578E-4
44-45	2.365415978374421E-4	6.150081543773494E-4	0.0	0.16203099451864783	2.3128511788549893E-4
46-47	2.4179807778938525E-4	6.728294338487242E-4	0.0	0.1887233997146152	2.575675176452148E-4
48-49	2.470545577413284E-4	6.833423937526106E-4	0.0	0.21017509439849524	2.733369575010442E-4
50-51	2.9961935726076003E-4	7.779590328875873E-4	0.0	0.22753724767976347	2.785934374529874E-4
52-53	3.0487583721270316E-4	7.884719927914736E-4	0.0	0.24781674733436018	2.8910639735687366E-4
54-55	3.0487583721270316E-4	7.884719927914736E-4	0.0	0.28147924494660415	2.9436287730881685E-4
56-57	3.0487583721270316E-4	7.9898495269536E-4	0.0	0.31666086526495973	2.9961935726076003E-4
58-59	3.0487583721270316E-4	7.9898495269536E-4	0.0	0.34423635909285355	3.101323171646463E-4
60-61	3.311582369724189E-4	8.200108725031326E-4	0.0	0.35979553975060524	3.153887971165895E-4
62-63	3.6795359663602104E-4	8.357803123589621E-4	0.0	0.3690732268657849	3.469276768282484E-4
64-65	3.6795359663602104E-4	9.566793512536547E-4	0.0	0.3774362864693265	3.469276768282484E-4
66-67	3.8372303649185054E-4	9.724487911094842E-4	0.0	0.38338136529497424	3.626971166840779E-4
68-69	3.8897951644379367E-4	9.882182309653136E-4	0.0	0.38908990252278447	3.732100765879642E-4
70-71	4.0474895629962317E-4	0.001003987670821143	0.0	0.39414137975660185	3.8897951644379367E-4
72-73	4.6782671572294105E-4	0.0010092441507730864	0.0	0.3982782294787811	4.5205727586711156E-4
74-75	4.730831956748842E-4	0.0010355265505328022	0.0	0.40157929888860144	4.5205727586711156E-4
76-77	4.835961555787705E-4	0.0010723219101964041	0.0	0.4046070313409207	4.6257023577099787E-4
78-79	4.835961555787705E-4	0.0010828348701002904	0.0	0.4069724473192951	4.6257023577099787E-4
80-81	4.835961555787705E-4	0.0010828348701002904	0.0	0.40817092474833816	4.6782671572294105E-4
82-83	4.835961555787705E-4	0.0010828348701002904	0.0	0.40867554682372464	4.835961555787705E-4
84-85	4.835961555787705E-4	0.0010880913500522335	0.0	0.40892785786141794	4.835961555787705E-4
86-87	4.941091154826568E-4	0.0011038607899080631	0.0	0.4090067050606971	4.835961555787705E-4
88-89	5.151350352904294E-4	0.001172195029283324	0.0	0.4090277309805049	4.835961555787705E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10815	0.0	54.216682	1
GTATCAA	19785	0.0	41.29604	1
ATCAACG	23920	0.0	33.292034	3
TCAACGC	23995	0.0	33.224678	4
TATCAAC	25430	0.0	31.741077	2
CAACGCA	25035	0.0	30.302773	5
AACGCAG	25925	0.0	29.395601	6
CTTATAC	11245	0.0	27.325188	1
ACGCAGA	29815	0.0	25.59754	7
CGCAGAG	30540	0.0	24.943144	8
TATACAC	15470	0.0	22.834875	3
GTACATG	23180	0.0	21.890476	1
GTGGTAT	5450	0.0	21.120998	1
GCAGAGT	35830	0.0	21.019857	9
TTATACA	15630	0.0	20.891552	2
TACATGG	23705	0.0	20.890343	2
ACATGGG	24215	0.0	19.785975	3
GAGTACT	22470	0.0	19.514206	12-13
AGAGTAC	32230	0.0	19.098955	10-11
TGGTATC	5275	0.0	19.060583	2
>>END_MODULE