FastQCFastQC Report
Fri 27 May 2016
SRR2138730_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138730_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6154681
Sequences flagged as poor quality0
Sequence length19-100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA78250.048.4536481
GTATCAA135100.033.1506771
CTTATAC49050.028.6779581
TCAACGC162200.027.213214
ATCAACG167300.026.3594783
CAACGCA169550.026.0096765
TATCAAC170300.025.9188652
AACGCAG173200.025.5315676
TATACAC65150.024.8172453
GTACATG224000.023.4947571
TACATGG226950.022.7084772
ACATGGG230900.021.829843
GTGGTAT40150.021.4438151
ACGCAGA207000.021.4407527
CGCAGAG213050.020.7749798
TTATACA74700.020.3458232
TGGTATC41300.019.6722492
CATGGGG172800.019.4152224
TACACAT83150.018.8129435
ACACATC76950.018.2275736
Produced by FastQC (version 0.11.5)