##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138730_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6154681
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.12952954019875	33.0	31.0	34.0	30.0	34.0
2	32.4181740694603	34.0	31.0	34.0	31.0	34.0
3	32.19964901511549	33.0	31.0	34.0	30.0	34.0
4	36.040744759964	37.0	35.0	37.0	35.0	37.0
5	35.87301990143762	37.0	35.0	37.0	35.0	37.0
6	35.9770181427762	37.0	35.0	37.0	35.0	37.0
7	35.94253073392431	37.0	35.0	37.0	35.0	37.0
8	35.91919613705406	37.0	35.0	37.0	35.0	37.0
9	37.7012426801649	39.0	37.0	39.0	35.0	39.0
10-11	37.62049966521417	39.0	37.0	39.0	35.0	39.0
12-13	37.59085986747323	39.0	37.0	39.0	35.0	39.0
14-15	38.89205338180809	40.0	38.0	41.0	35.0	41.0
16-17	38.871378792824515	40.0	38.0	41.0	35.0	41.0
18-19	38.84348920764537	40.0	38.0	41.0	35.0	41.0
20-21	38.83882680837836	40.0	38.0	41.0	35.0	41.0
22-23	38.74562118162292	40.0	38.0	41.0	34.0	41.0
24-25	38.67174881700355	40.0	38.0	41.0	34.0	41.0
26-27	38.511153090335924	40.0	38.0	41.0	34.0	41.0
28-29	38.385583270782746	40.0	38.0	41.0	34.0	41.0
30-31	38.23334671035593	40.0	38.0	41.0	34.0	41.0
32-33	38.06232132953796	40.0	37.5	41.0	33.0	41.0
34-35	38.150803591170785	40.0	38.0	41.0	33.0	41.0
36-37	38.14245135866027	40.0	38.0	41.0	33.0	41.0
38-39	38.06107610539535	40.0	37.0	41.0	33.0	41.0
40-41	38.04580357944463	40.0	37.0	41.0	33.0	41.0
42-43	38.11725383807507	40.0	37.0	41.0	33.0	41.0
44-45	38.06880875331679	40.0	37.0	41.0	33.0	41.0
46-47	37.96981742025367	40.0	37.0	41.0	33.0	41.0
48-49	37.85962872309942	40.0	37.0	41.0	33.0	41.0
50-51	37.72923345615645	40.0	37.0	41.0	33.0	41.0
52-53	37.56189982566404	39.5	36.0	41.0	32.0	41.0
54-55	37.37113428219935	39.0	36.0	41.0	32.0	41.0
56-57	37.236684789344764	39.0	35.5	41.0	32.0	41.0
58-59	37.002847529154096	39.0	35.0	40.5	31.0	41.0
60-61	36.76720259945957	38.0	35.0	40.0	31.0	41.0
62-63	36.475926406784666	38.0	35.0	40.0	31.0	41.0
64-65	36.14907503187716	37.0	35.0	40.0	30.5	41.0
66-67	35.84040457119404	37.0	34.0	39.5	30.0	41.0
68-69	35.545092971578555	36.0	34.0	39.0	30.0	41.0
70-71	35.23363103251744	36.0	34.0	39.0	30.0	40.5
72-73	34.77786479486008	35.0	34.0	38.5	29.0	40.0
74-75	34.54942514882221	35.0	34.0	37.0	29.0	39.5
76-77	33.36412636020455	34.5	32.0	36.0	28.0	39.0
78-79	34.10049423338509	35.0	33.5	36.0	29.0	39.0
80-81	34.045715971294726	35.0	34.0	36.0	29.0	38.5
82-83	33.84019457211026	35.0	33.0	36.0	29.0	37.0
84-85	33.64995958794884	35.0	33.0	35.0	29.0	37.0
86-87	33.48906857848831	35.0	33.0	35.0	29.0	36.5
88-89	33.33815286088617	35.0	33.0	35.0	29.0	36.0
90-91	33.20569290344099	35.0	33.0	35.0	29.0	36.0
92-93	33.13887245215159	35.0	33.0	35.0	29.0	36.0
94-95	33.11255425092633	35.0	33.0	35.0	29.0	35.0
96-97	33.17688368518891	35.0	33.0	35.0	29.0	35.0
98-99	33.28227240209296	35.0	33.0	35.0	29.5	35.0
100	32.064022951148694	34.0	31.0	35.0	26.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	2.0
19	9.0
20	56.0
21	221.0
22	923.0
23	3225.0
24	8379.0
25	17765.0
26	31564.0
27	50737.0
28	72079.0
29	96478.0
30	123326.0
31	153399.0
32	191573.0
33	243864.0
34	330671.0
35	468823.0
36	753425.0
37	1293350.0
38	1460018.0
39	819588.0
40	35205.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.8335440943243	19.48984845843351	13.198913152444455	24.477694294797733
2	15.876744871098925	20.68113684527273	39.647026385283006	23.79509189834534
3	18.932955257957317	25.25796219170417	29.820278256501027	25.988804293837486
4	13.247494061836838	17.176373560221887	36.92704788436639	32.64908449357489
5	13.79871678158462	37.39914383864899	34.86354532428245	13.938594055483946
6	31.628446705848766	36.27578748598018	17.942830830712428	14.15293497745862
7	28.071706722086816	31.743871047094075	21.866819742566673	18.317602488252437
8	25.867189542398705	35.46347893578887	20.48705692463996	18.18227459717246
9	26.257234127975117	16.02781687629302	20.367391908695186	37.347557087036684
10-11	25.39603596027154	25.90710225274064	27.863353112858324	20.833508674129497
12-13	25.91404006154015	23.34734326604417	28.068798366641584	22.669818305774093
14-15	23.025198219046608	24.686827798223824	25.57100034916513	26.71697363356444
16-17	22.86384135912162	27.84241782799141	26.16781438388115	23.125926429005826
18-19	22.876539011526347	26.979554261220034	27.782512204937998	22.361394522315617
20-21	23.687728703141524	26.15621754465843	27.61245111920378	22.54360263299627
22-23	23.419385170062302	26.237966477501807	27.437828865633257	22.904819486802637
24-25	23.09397550019952	26.376880497440386	27.66179937730554	22.867344625054553
26-27	23.118567221267263	26.474036333358814	27.665937028899833	22.741459416474093
28-29	23.02183489554965	26.37093402819061	27.729422978906804	22.877808097352936
30-31	23.362171059660035	26.429652220021598	27.376392676733285	22.83178404358508
32-33	23.035832152985225	26.503711397380247	27.489852660972797	22.970603788661737
34-35	23.04386106804173	26.60630706692916	27.648462784767514	22.7013690802616
36-37	23.220425491638125	26.488900957367356	27.37583484099683	22.91483870999769
38-39	22.870134705595536	26.55752144243212	27.617795777945155	22.954548074027194
40-41	23.247039891408182	26.437996401933876	27.41877619335002	22.896187513307925
42-43	23.15849637927927	26.402035065195488	27.29539552340587	23.14407303211937
44-45	23.055288916304498	26.70231808782341	27.125885223365504	23.116507772506587
46-47	23.35766640627305	26.43968444250713	26.826247711633872	23.376401439585955
48-49	23.191853640705812	26.583560980332592	26.747246738109798	23.477338640851794
50-51	23.052279969589623	26.748129456189822	26.870175362920545	23.32941521130001
52-53	23.322597560141904	26.646702395203487	26.833475394146028	23.197224650508584
54-55	23.257416446458297	26.52403707687407	26.729001725025604	23.48954475164203
56-57	23.364260439936732	26.81850222111445	26.510681615415255	23.306555723533563
58-59	23.142282814255484	26.996474587335562	26.750013033297996	23.111229565110957
60-61	23.348833806875575	27.091888898314835	26.503952228182698	23.055325066626885
62-63	23.22026424491822	27.28758136628877	26.383075248972617	23.10907913982039
64-65	23.202811197553782	27.16537711205899	26.45867087700735	23.173140813379877
66-67	23.34709325864887	27.058546995441073	26.308442429931617	23.285917315978438
68-69	23.28139430549736	26.887876788138716	26.507769036313146	23.322959870050784
70-71	23.285069028078144	26.56294159736648	26.599418634973386	23.552570739581988
72-73	23.407819645775803	26.620934050138207	26.493380375265275	23.47786592882072
74-75	23.149725418329634	26.826248552977816	26.566107946635498	23.457918082057052
76-77	23.354688065559344	26.660001019418196	26.49649623385195	23.48881468117051
78-79	23.24996783645928	26.683877298192744	26.455104225694537	23.61105063965344
80-81	23.34407730962882	26.699647573781654	26.42993781633199	23.526337300257538
82-83	23.36838565656186	26.56387194406103	26.6272393252089	23.440503074168216
84-85	23.493617610575843	26.617153494227132	26.47016007233714	23.41906882285989
86-87	23.23348024992087	26.64292344023711	26.624692490707908	23.498903819134107
88-89	23.351385490340874	26.53777031683433	26.60783193346062	23.50301225936418
90-91	23.37331227351535	26.531248372781924	26.645322386856513	23.450116966846217
92-93	23.43056403120636	26.53645114820757	26.608140700464	23.424844120122067
94-95	23.24539261971276	26.50377261673638	26.756676756079216	23.49415800747164
96-97	23.225302565590418	26.641586707837696	26.8311305798483	23.30198014672359
98-99	24.47127552600058	28.671065686235504	26.920228516274275	19.937430271489646
100	31.90963749194195	40.192757272275635	27.897605235782418	0.0
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1249.0
1	1143.0
2	1605.5
3	2318.0
4	3108.5
5	5057.0
6	6805.5
7	8175.0
8	9074.5
9	8885.5
10	7984.0
11	6945.0
12	7304.5
13	9535.0
14	14936.5
15	22184.0
16	27914.5
17	31313.0
18	32218.0
19	30762.0
20	28125.0
21	25489.0
22	24061.5
23	25271.5
24	29255.0
25	35983.5
26	45461.0
27	55341.0
28	64706.0
29	76063.0
30	86752.5
31	95897.5
32	107762.5
33	121828.0
34	134358.5
35	147402.5
36	162760.0
37	174887.0
38	183487.5
39	190737.0
40	198953.0
41	207505.0
42	214335.5
43	224029.5
44	236618.5
45	249915.5
46	260809.5
47	268662.0
48	274101.5
49	277931.5
50	281389.5
51	282225.0
52	278870.0
53	272686.5
54	265507.5
55	256201.0
56	243385.0
57	225428.5
58	205248.5
59	183007.0
60	158769.5
61	134172.0
62	110637.5
63	88402.5
64	68124.5
65	53447.5
66	41485.5
67	30698.5
68	22594.5
69	16592.5
70	11677.0
71	7831.5
72	5238.5
73	3489.5
74	2312.5
75	1574.5
76	1054.5
77	640.5
78	384.0
79	258.5
80	185.5
81	120.0
82	82.5
83	53.5
84	34.0
85	25.0
86	21.5
87	18.0
88	11.5
89	5.5
90	3.5
91	3.0
92	2.0
93	1.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.005353841824277325
22-23	0.004680014566545338
24-25	0.0034294968586783985
26-27	0.01634099842687403
28-29	0.02046294920648507
30-31	0.038485684116254644
32-33	0.023722702225197647
34-35	0.012467195537648013
36-37	0.007172728739312262
38-39	0.002981564659990133
40-41	0.007578032852375785
42-43	0.0096373811670391
44-45	0.006207845577121559
46-47	0.0020485551339286695
48-49	0.009849460316125084
50-51	0.004690742576899872
52-53	1.8181841322343502E-5
54-55	5.519168393783099E-4
56-57	7.081426434089018E-4
58-59	4.627743815280111E-4
60-61	0.0011298244942120432
62-63	0.001116206404617639
64-65	0.0
66-67	1.198743357338502E-4
68-69	7.103439576773011E-4
70-71	3.092138300214471E-4
72-73	5.438021664450847E-4
74-75	4.135089339665463E-4
76-77	0.0
78-79	1.091214154706232E-5
80-81	3.7688944934567004E-4
82-83	0.0019706900951314047
84-85	3.430683945725665E-5
86-87	6.390668044417234E-4
88-89	2.4827713403063737E-4
90-91	0.008509023783565146
92-93	7.995510705250787E-4
94-95	5.031914287378411E-5
96-97	2.9790187707972747E-4
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	137.0
20-21	509.0
22-23	1077.0
24-25	2080.0
26-27	3811.0
28-29	8853.0
30-31	19982.0
32-33	26271.0
34-35	35720.0
36-37	53864.0
38-39	61833.0
40-41	61776.0
42-43	68280.0
44-45	79137.0
46-47	77855.0
48-49	72793.0
50-51	65070.0
52-53	63374.0
54-55	68163.0
56-57	71850.0
58-59	71817.0
60-61	71805.0
62-63	70030.0
64-65	73624.0
66-67	77943.0
68-69	77421.0
70-71	71921.0
72-73	65722.0
74-75	65866.0
76-77	66388.0
78-79	71316.0
80-81	71259.0
82-83	67903.0
84-85	68255.0
86-87	72687.0
88-89	78672.0
90-91	83598.0
92-93	87605.0
94-95	100870.0
96-97	224309.0
98-99	1251744.0
100-101	2421491.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.39891591765509
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.43894114579275	77.23387530591312
2	6.561629863819881	11.207121540462573
3	1.6633126911037421	4.2613530195701115
4	0.6169533294021425	2.1074858201092375
5	0.2740986959284922	1.1703865743368105
6	0.14382671305047218	0.7369587224705987
7	0.0849736742671797	0.5079661764769963
8	0.05516477144096118	0.3768809342322644
9	0.034226946303684155	0.26306496985557765
>10	0.12187879021546116	1.6793068892281535
>50	0.003501770402602448	0.20531406577583822
>100	0.001428295639374397	0.19816413802928512
>500	3.165631662218224E-5	0.022519447961931796
>1k	3.165631661931081E-5	0.02960239557752535
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	9.74867747004272E-5	0.0	0.0	1.6247795783404534E-5
5	0.0	1.2998236626723627E-4	0.0	1.6247795783404534E-5	1.6247795783404534E-5
6	0.0	1.2998236626723627E-4	0.0	1.6247795783404534E-5	6.499118313361814E-5
7	0.0	1.462301620506408E-4	0.0	1.6247795783404534E-5	1.1373457048383174E-4
8	0.0	2.5996473253447254E-4	0.0	1.6247795783404534E-5	1.2998236626723627E-4
9	0.0	6.336640355527768E-4	0.0	1.6247795783404534E-5	1.2998236626723627E-4
10-11	8.123897891702267E-6	6.336640355527768E-4	0.0	1.6247795783404534E-5	1.462301620506408E-4
12-13	1.6247795783404534E-5	6.336640355527768E-4	0.0	1.6247795783404534E-5	1.462301620506408E-4
14-15	1.6247795783404534E-5	8.20513687061929E-4	0.0	1.6247795783404534E-5	1.7060185572574763E-4
16-17	1.6247795783404534E-5	8.286375849536312E-4	0.0	1.6247795783404534E-5	1.949735494008544E-4
18-19	1.6247795783404534E-5	8.53009278628738E-4	0.0	1.6247795783404534E-5	2.3559303885936574E-4
20-21	1.6247795783404534E-5	9.017526659789517E-4	0.0	3.249559156680907E-5	2.518408346427703E-4
22-23	1.6247795783404534E-5	9.911155427876767E-4	0.0	7.311508102532041E-5	3.3307981355979295E-4
24-25	1.6247795783404534E-5	0.0011373457048383174	0.0	1.1373457048383174E-4	4.0619489458511336E-4
26-27	1.6247795783404534E-5	0.0011860890921885309	0.0	2.193452430759612E-4	4.0619489458511336E-4
28-29	1.6247795783404534E-5	0.0011860890921885309	0.0	7.230269123615018E-4	4.0619489458511336E-4
30-31	1.6247795783404534E-5	0.0014135582331561945	0.0	0.00218532853286791	4.143187924768156E-4
32-33	1.6247795783404534E-5	0.001429806028939599	0.0	0.0047281085729707194	4.87433873502136E-4
34-35	1.6247795783404534E-5	0.001673522965690667	0.0	0.008318871441103121	5.524250566357542E-4
36-37	1.6247795783404534E-5	0.0019172399024417349	0.0	0.014070591148428326	5.524250566357542E-4
38-39	3.249559156680907E-5	0.0019822310855753533	0.0	0.023104365604001247	5.849206482025633E-4
40-41	3.249559156680907E-5	0.0022097002265430168	0.0	0.031634458390288625	6.011684439859678E-4
42-43	3.249559156680907E-5	0.0022909392054600393	0.0	0.03967711730307387	6.011684439859678E-4
44-45	3.249559156680907E-5	0.0025996473253447256	0.0	0.04948266205835851	6.011684439859678E-4
46-47	3.249559156680907E-5	0.0028108686705289846	0.0	0.059726897299795065	6.255401376610745E-4
48-49	3.249559156680907E-5	0.0028108686705289846	0.0	0.06935371630146225	6.336640355527768E-4
50-51	3.249559156680907E-5	0.0030545856072800525	0.0	0.07876119006005347	6.336640355527768E-4
52-53	3.249559156680907E-5	0.0030870811988468615	0.0	0.0885342392237713	7.230269123615018E-4
54-55	3.249559156680907E-5	0.0031439484840887775	0.0	0.09815293432754679	8.123897891702267E-4
56-57	3.249559156680907E-5	0.003200815769330693	0.0	0.10764164706505504	8.123897891702267E-4
58-59	3.249559156680907E-5	0.003217063565114098	0.0	0.11669979321430307	8.123897891702267E-4
60-61	3.249559156680907E-5	0.003347045931381334	0.0	0.1257660632614428	8.123897891702267E-4
62-63	3.249559156680907E-5	0.003412037114514952	0.0	0.13447488180134765	8.286375849536312E-4
64-65	3.249559156680907E-5	0.0037857364175332563	0.0	0.1422494520837067	8.286375849536312E-4
66-67	3.249559156680907E-5	0.0037938603154249583	0.0	0.1508851555425862	8.367614828453335E-4
68-69	4.061948945851133E-5	0.0038832231922336833	0.0	0.15930963765628145	8.448853807370357E-4
70-71	4.87433873502136E-5	0.003972586069042409	0.0	0.1660118534169358	8.448853807370357E-4
72-73	4.87433873502136E-5	0.004005081660609217	0.0	0.17128426314865058	8.773809723038448E-4
74-75	4.87433873502136E-5	0.004094444537417943	0.0	0.17537870768606853	8.936287680872493E-4
76-77	4.87433873502136E-5	0.004216303005793476	0.0	0.17970874526234587	8.936287680872493E-4
78-79	5.686728524191587E-5	0.004240674699468584	0.0	0.18260897680968358	9.09876563870654E-4
80-81	6.499118313361814E-5	0.0042569224952519885	0.0	0.1833969949051787	9.180004617623562E-4
82-83	6.499118313361814E-5	0.004273170291035393	0.0	0.18403065894073145	9.74867747004272E-4
84-85	6.499118313361814E-5	0.004297541984710499	0.0	0.18430687146904934	9.74867747004272E-4
86-87	6.499118313361814E-5	0.0043544092699524155	0.0	0.18442060603953317	9.911155427876767E-4
88	6.499118313361814E-5	0.0044843916362196515	0.0	0.18442872993742487	0.0010073633385710812
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7825	0.0	48.453648	1
GTATCAA	13510	0.0	33.150677	1
CTTATAC	4905	0.0	28.677958	1
TCAACGC	16220	0.0	27.21321	4
ATCAACG	16730	0.0	26.359478	3
CAACGCA	16955	0.0	26.009676	5
TATCAAC	17030	0.0	25.918865	2
AACGCAG	17320	0.0	25.531567	6
TATACAC	6515	0.0	24.817245	3
GTACATG	22400	0.0	23.494757	1
TACATGG	22695	0.0	22.708477	2
ACATGGG	23090	0.0	21.82984	3
GTGGTAT	4015	0.0	21.443815	1
ACGCAGA	20700	0.0	21.440752	7
CGCAGAG	21305	0.0	20.774979	8
TTATACA	7470	0.0	20.345823	2
TGGTATC	4130	0.0	19.672249	2
CATGGGG	17280	0.0	19.415222	4
TACACAT	8315	0.0	18.812943	5
ACACATC	7695	0.0	18.227573	6
>>END_MODULE