FastQCFastQC Report
Fri 27 May 2016
SRR2138729_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138729_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10292861
Sequences flagged as poor quality0
Sequence length19-100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA140700.051.5778161
GTATCAA261100.037.053421
TCAACGC334750.028.4656054
ATCAACG341550.027.8633023
TATCAAC344500.027.7892782
CAACGCA344100.027.656825
AACGCAG350050.027.3139786
GTACATG456100.023.0318571
ACGCAGA416700.022.9063117
TACATGG460300.022.469792
CGCAGAG430500.022.1626248
GTGGTAT65800.021.7869511
ACATGGG471000.021.5466233
TGGTATC69300.019.4594272
CATGGGG352850.019.407634
GCAGAGT491750.019.2786279
GAGTACT234000.018.15426412-13
CAGAGTA470850.017.52833610-11
AGAGTAC442500.017.11841210-11
GTACTTT252300.016.847614-15
Produced by FastQC (version 0.11.5)