##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138729_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10292861
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.16436411606064	33.0	31.0	34.0	31.0	34.0
2	32.44890405106996	34.0	31.0	34.0	31.0	34.0
3	32.22582700767066	33.0	31.0	34.0	30.0	34.0
4	36.064056825405494	37.0	35.0	37.0	35.0	37.0
5	35.903454637150936	37.0	35.0	37.0	35.0	37.0
6	36.00614999075573	37.0	35.0	37.0	35.0	37.0
7	35.97183737349606	37.0	35.0	37.0	35.0	37.0
8	35.94936480731645	37.0	35.0	37.0	35.0	37.0
9	37.73192973265645	39.0	37.0	39.0	35.0	39.0
10-11	37.65416403660751	39.0	37.0	39.0	35.0	39.0
12-13	37.62274488113654	39.0	37.0	39.0	35.0	39.0
14-15	38.92533936871391	40.0	38.0	41.0	35.0	41.0
16-17	38.90468058395037	40.0	38.0	41.0	35.0	41.0
18-19	38.886589744095446	40.0	38.0	41.0	35.0	41.0
20-21	38.88354383784683	40.0	38.0	41.0	35.0	41.0
22-23	38.79267512595648	40.0	38.0	41.0	35.0	41.0
24-25	38.720103928260514	40.0	38.0	41.0	34.0	41.0
26-27	38.566736133123705	40.0	38.0	41.0	34.0	41.0
28-29	38.443306759930024	40.0	38.0	41.0	34.0	41.0
30-31	38.28785463584638	40.0	38.0	41.0	34.0	41.0
32-33	38.117356732336006	40.0	37.5	41.0	33.5	41.0
34-35	38.20282041600005	40.0	38.0	41.0	34.0	41.0
36-37	38.19254696244348	40.0	38.0	41.0	33.5	41.0
38-39	38.12565679120429	40.0	37.5	41.0	33.0	41.0
40-41	38.09195054166351	40.0	37.0	41.0	33.0	41.0
42-43	38.1543424306263	40.0	37.0	41.0	33.0	41.0
44-45	38.10461151297072	40.0	37.0	41.0	33.0	41.0
46-47	38.00036034831591	40.0	37.0	41.0	33.0	41.0
48-49	37.88621639720682	40.0	37.0	41.0	33.0	41.0
50-51	37.759052983213536	40.0	37.0	41.0	33.0	41.0
52-53	37.58229771617896	39.5	36.0	41.0	32.0	41.0
54-55	37.394384749730776	39.0	36.0	41.0	32.0	41.0
56-57	37.26001139133446	39.0	35.0	41.0	32.0	41.0
58-59	37.022157337645666	39.0	35.0	40.5	31.5	41.0
60-61	36.79243027568734	38.0	35.0	40.0	31.0	41.0
62-63	36.49595664886213	38.0	35.0	40.0	31.0	41.0
64-65	36.16204571540693	37.0	35.0	40.0	31.0	41.0
66-67	35.84770952181943	37.0	34.0	39.5	30.0	41.0
68-69	35.549940283793774	36.0	34.0	39.0	30.0	41.0
70-71	35.235357555639986	36.0	34.0	39.0	30.0	40.5
72-73	34.77994399112528	35.0	34.0	38.0	29.0	40.0
74-75	34.55377279563332	35.0	34.0	37.0	29.0	39.5
76-77	33.372643106465205	34.5	32.0	36.0	28.0	39.0
78-79	34.098431611232506	35.0	33.5	36.0	29.5	39.0
80-81	34.04850671118092	35.0	34.0	36.0	30.0	37.5
82-83	33.848968335667934	35.0	34.0	36.0	29.0	37.0
84-85	33.66826757880746	35.0	33.0	35.0	29.0	37.0
86-87	33.51874095474859	35.0	33.0	35.0	29.0	36.0
88-89	33.37718109025233	35.0	33.0	35.0	29.0	36.0
90-91	33.25360994480086	35.0	33.0	35.0	29.0	36.0
92-93	33.18934649398858	35.0	33.0	35.0	29.0	35.5
94-95	33.167506232313954	35.0	33.0	35.0	29.0	35.0
96-97	33.22390202281176	35.0	33.0	35.0	29.0	35.0
98-99	33.32514680566874	35.0	33.0	35.0	29.5	35.0
100	32.14562263454623	34.0	31.0	35.0	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	1.0
17	0.0
18	1.0
19	15.0
20	70.0
21	348.0
22	1538.0
23	5047.0
24	13414.0
25	28285.0
26	50960.0
27	81312.0
28	117186.0
29	156215.0
30	199084.0
31	249284.0
32	311020.0
33	398604.0
34	549108.0
35	785046.0
36	1273352.0
37	2201661.0
38	2448632.0
39	1362373.0
40	60304.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.31878182363485	19.306906019618843	13.434768039712186	23.939544117034124
2	15.96330699501334	20.759602213611938	39.597416112002286	23.679674679372432
3	18.92339748880316	25.639936262619305	29.666241485239137	25.770424763338397
4	13.425926960443748	17.098802752704035	36.96903125379814	32.50623903305407
5	13.912934411530479	37.60655079282621	34.42589965996821	14.054615135675105
6	31.547457990543155	36.0292148120916	18.167776675503536	14.255550521861707
7	28.215828427101076	31.937631334961193	22.019057675023497	17.827482562914238
8	26.21893951545639	35.09060308887879	20.394222753032416	18.296234642632403
9	26.858489588074686	15.942088404769091	20.419871598382606	36.779550408773616
10-11	25.689703766523227	25.824418497442064	27.574111804288425	20.911765931746285
12-13	26.21926984149499	23.49258869812776	27.90376261760457	22.384378842772676
14-15	23.429613010415665	24.573614663600335	25.641733624888165	26.35503870109584
16-17	22.92923221250146	27.593941082076206	26.29655641905589	23.180270286366444
18-19	22.9156791294471	26.84354233482799	27.991128997078658	22.249649538646253
20-21	23.944278749663816	25.908762675845043	27.807962988009344	22.338995586481794
22-23	23.81486247397922	25.765566738679063	27.633291152678503	22.786279634663217
24-25	23.27903404535373	26.014747030448714	27.75781292634809	22.94840599784946
26-27	23.165075088497495	26.15007119716995	27.76629824941069	22.918555464921873
28-29	23.16454275167141	26.18095929967325	27.731992018171713	22.922505930483624
30-31	23.436758724729927	26.10075499415794	27.52086804554727	22.941618235564867
32-33	23.12970958411605	26.370999830186747	27.49099918831125	23.008291397385953
34-35	23.315450270467156	26.364429603155614	27.506600247793088	22.81351987858414
36-37	23.345278971825056	26.26604347158607	27.410734634510746	22.977942922078128
38-39	23.15319782844205	26.439802284674485	27.513960108100672	22.893039778782793
40-41	23.43772899122707	26.210129324533614	27.37984016037136	22.97230152386796
42-43	23.210953763294675	26.34285875137265	27.16445831135041	23.281729173982267
44-45	23.254772872269786	26.48702205444225	27.010191063596096	23.248014009691868
46-47	23.490097621588916	26.411209308125205	26.769720900050636	23.328972170235243
48-49	23.347998626151007	26.557756352511642	26.6417049406524	23.45254008068495
50-51	23.336240740639155	26.68428751257268	26.592348689241184	23.38712305754698
52-53	23.543773473044197	26.582836231497975	26.379593621426046	23.493796674031785
54-55	23.50358224378637	26.43291540718552	26.48386813511089	23.579634213917224
56-57	23.441690578415194	26.597511736023886	26.477683058751733	23.483114626809186
58-59	23.406121065399375	26.67315812716507	26.463456613466292	23.45726419396927
60-61	23.540860162432708	26.63452240210838	26.479478983597897	23.34513845186102
62-63	23.54751570187089	26.67631136792434	26.36973881440098	23.406434115803787
64-65	23.506553101687942	26.65741463029385	26.39354663123217	23.442485636786035
66-67	23.613383364230685	26.62119449263373	26.218723497957203	23.546698645178378
68-69	23.419804168139557	26.63495240385637	26.37642363465324	23.568819793350833
70-71	23.427945656418853	26.359128534839154	26.532584392439606	23.68034141630239
72-73	23.599536359499865	26.474470378599555	26.275598631798175	23.650394630102404
74-75	23.303408035885656	26.62064345207632	26.414404772991894	23.66154373904613
76-77	23.547101130184775	26.416955468656827	26.360861010625676	23.675082390532722
78-79	23.396144232062603	26.435101551514073	26.339770336018052	23.828983880405275
80-81	23.555443457591423	26.516812948579915	26.24299434395051	23.684749249878156
82-83	23.533056237131404	26.400867371048616	26.447332720891325	23.61874367092866
84-85	23.56420975100802	26.389985797390747	26.299784170103894	23.746020281497334
86-87	23.362638375166746	26.512112461295427	26.468646335829106	23.65660282770872
88-89	23.50335503217583	26.310543962203443	26.44851315601685	23.73758784960388
90-91	23.552372276590543	26.265489849627105	26.4899879505981	23.692149923184257
92-93	23.60590530994764	26.32684736454146	26.4031689310851	23.664078394425793
94-95	23.436335989658637	26.27736369987634	26.544565367645134	23.74173494281989
96-97	23.450362878745537	26.38289117988965	26.629817706121074	23.53692823524374
98-99	24.603035101922163	28.430925995095297	26.733690848216174	20.232348054766366
100	32.29904439593574	39.98299032617743	27.717965277886837	0.0
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2638.0
1	2410.0
2	3362.5
3	4737.0
4	6153.0
5	9903.5
6	13288.0
7	15901.5
8	17811.5
9	17605.5
10	15702.0
11	13630.0
12	14376.5
13	18714.0
14	29170.5
15	43212.5
16	54326.0
17	60197.5
18	60657.5
19	56927.0
20	50360.5
21	43264.0
22	38875.5
23	39119.0
24	43831.5
25	53077.0
26	66758.0
27	81662.0
28	96670.5
29	115197.5
30	133473.0
31	150356.0
32	171490.5
33	194580.0
34	214269.0
35	236273.0
36	262708.0
37	284567.5
38	302315.0
39	314650.0
40	325624.5
41	340026.0
42	354561.0
43	372204.5
44	392630.5
45	410349.0
46	423070.0
47	435999.5
48	450416.5
49	462706.0
50	470617.0
51	476061.0
52	475970.0
53	469314.5
54	460216.5
55	444280.0
56	417829.5
57	386093.0
58	352924.5
59	314007.5
60	272467.0
61	230950.0
62	189897.0
63	151537.5
64	117538.5
65	91213.0
66	69655.5
67	51734.5
68	38512.0
69	28170.0
70	19461.5
71	12802.0
72	8449.0
73	5517.0
74	3596.0
75	2394.0
76	1629.0
77	1077.5
78	719.5
79	478.5
80	335.5
81	236.0
82	167.5
83	115.0
84	76.0
85	53.0
86	39.0
87	32.0
88	23.5
89	14.0
90	8.0
91	6.0
92	5.5
93	3.0
94	0.5
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.004935621233431178
22-23	0.004766049172607062
24-25	0.0037611370840681686
26-27	0.016912149670823166
28-29	0.021469655053235795
30-31	0.039838202208634224
32-33	0.02414277851392492
34-35	0.012706479056273192
36-37	0.0072254203045784195
38-39	0.002640647289041109
40-41	0.00786942683786302
42-43	0.009412717795298781
44-45	0.006159531239536735
46-47	0.001953346748059955
48-49	0.00961049665878606
50-51	0.004372213822875003
52-53	5.441131877311835E-6
54-55	4.4593760749298504E-4
56-57	6.634494016689934E-4
58-59	4.405949251598682E-4
60-61	0.001018807122240199
62-63	9.102703073768306E-4
64-65	0.0
66-67	2.0829632354013288E-4
68-69	6.277861026507544E-4
70-71	3.491277716380971E-4
72-73	6.400743331373131E-4
74-75	3.46565476268866E-4
76-77	0.0
78-79	6.485557474199641E-6
80-81	3.557955638223117E-4
82-83	0.00222916245212154
84-85	4.079058961097675E-5
86-87	6.009105659969714E-4
88-89	1.9675714708039448E-4
90-91	0.008881714119253772
92-93	8.478477931947205E-4
94-95	8.219283919546361E-5
96-97	2.075719159810387E-4
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	205.0
20-21	775.0
22-23	1674.0
24-25	3492.0
26-27	6479.0
28-29	14515.0
30-31	33458.0
32-33	43473.0
34-35	58095.0
36-37	87953.0
38-39	104647.0
40-41	105066.0
42-43	115691.0
44-45	135351.0
46-47	133509.0
48-49	123095.0
50-51	110189.0
52-53	105500.0
54-55	112324.0
56-57	116592.0
58-59	115926.0
60-61	114101.0
62-63	107990.0
64-65	112065.0
66-67	117632.0
68-69	119658.0
70-71	119601.0
72-73	111235.0
74-75	111751.0
76-77	111653.0
78-79	120322.0
80-81	120694.0
82-83	115262.0
84-85	114114.0
86-87	121243.0
88-89	131630.0
90-91	139812.0
92-93	144922.0
94-95	166474.0
96-97	369612.0
98-99	2119753.0
100-101	4075328.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.38617202723985
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.13530074089962	73.43516220539516
2	7.168477570818645	11.811668526457506
3	1.9256282813900811	4.759354285533643
4	0.7472342993237086	2.462470941149484
5	0.35941892027221006	1.4805574497695553
6	0.20606894461038988	1.0186338912086006
7	0.12176589653449521	0.7022278269259409
8	0.07681090326536522	0.5062525031990447
9	0.05239593852979846	0.38850307247201943
>10	0.19698028319294156	2.6096679391049586
>50	0.007137298843268628	0.40205720762133135
>100	0.0027330604140422114	0.3925297880396622
>500	4.786190541012947E-5	0.030914363122969614
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.886188689422698E-5
2	0.0	1.068701889591242E-4	0.0	0.0	3.886188689422698E-5
3	0.0	1.263011324062377E-4	0.0	0.0	3.886188689422698E-5
4	0.0	3.7890339721871304E-4	0.0	0.0	4.857735861778372E-5
5	0.0	3.983343406658265E-4	0.0	0.0	4.857735861778372E-5
6	0.0	3.983343406658265E-4	0.0	0.0	7.772377378845395E-5
7	0.0	3.983343406658265E-4	0.0	0.0	7.772377378845395E-5
8	0.0	4.0804981238938326E-4	0.0	0.0	7.772377378845395E-5
9	0.0	8.355305682258801E-4	0.0	0.0	1.3601660412979443E-4
10-11	0.0	8.355305682258801E-4	0.0	9.715471723556744E-6	1.408743399915728E-4
12-13	0.0	8.355305682258801E-4	0.0	9.715471723556744E-6	1.5058981171512954E-4
14-15	0.0	0.001199860757859258	0.0	9.715471723556744E-6	2.1374037791824837E-4
16-17	0.0	0.0012387226447534848	0.0	9.715471723556744E-6	2.3802905722714024E-4
18-19	0.0	0.0013261618902654956	0.0	1.4573207585335115E-5	2.428867930889186E-4
20-21	0.0	0.0014330320792246198	0.0	2.428867930889186E-5	2.47744528950697E-4
22-23	0.0	0.001593337362663306	0.0	3.886188689422698E-5	2.9632188756848073E-4
24-25	0.0	0.0018410818916140032	0.0	6.315056620311884E-5	3.3032603860092935E-4
26-27	0.0	0.001943094344711349	0.0	1.6516301930046467E-4	3.3032603860092935E-4
28-29	0.0	0.0019528098164349056	0.0	7.966686813316531E-4	3.400415103244861E-4
30-31	0.0	0.002292851326759392	0.0	0.0023219977419300618	3.5461471790982117E-4
32-33	0.0	0.002307424534344727	0.0	0.005226923787273529	3.886188689422698E-4
34-35	0.0	0.0026377505729456565	0.0	0.009015957759460659	3.934766048040481E-4
36-37	0.0	0.0029535034039612504	0.0	0.015049265699789396	3.983343406658265E-4
38-39	0.0	0.0030846622722292666	0.0	0.02369117779789312	4.0804981238938326E-4
40-41	0.0	0.003380984159797747	0.0	0.03264398499115066	4.1776528411294005E-4
42-43	0.0	0.0035024275563422065	0.0	0.0410818721830597	4.274807558364968E-4
44-45	0.0	0.003973627934934709	0.0	0.050505879754909735	4.3719622756005353E-4
46-47	0.0	0.004313669445259195	0.0	0.06019220506329581	4.5176943514538867E-4
48-49	0.0	0.0043233849169827514	0.0	0.069941680937885	4.6634264273072375E-4
50-51	0.0	0.004765438880404583	0.0	0.0797785960579862	4.6634264273072375E-4
52-53	0.0	0.0047800120879899185	0.0	0.08941148627189272	4.857735861778373E-4
54-55	0.0	0.0048043007672988105	0.0	0.09904923422166101	5.100622654867291E-4
56-57	0.0	0.004857735861778373	0.0	0.10867240896384397	5.246354730720642E-4
58-59	0.0	0.0048917400128108204	0.0	0.11821300219637669	5.246354730720642E-4
60-61	0.0	0.004974321522461054	0.0	0.12719009806894313	5.246354730720642E-4
62-63	0.0	0.005090907183143735	0.0	0.13550654186430772	5.246354730720642E-4
64-65	0.0	0.005634973599662912	0.0	0.14369668452726603	5.246354730720642E-4
66-67	0.0	0.005727270581036701	0.0	0.15218800681365463	5.246354730720642E-4
68-69	0.0	0.005838998505857604	0.0	0.16053359702418987	5.294932089338426E-4
70-71	0.0	0.005936153223093171	0.0	0.16768418421272763	5.392086806573994E-4
72-73	0.0	0.00599930378929629	0.0	0.1731054174344723	5.829283034134047E-4
74-75	0.0	0.006154751336873198	0.0	0.1772150619735368	5.829283034134047E-4
76-77	0.0	0.006426784545132787	0.0	0.18108667745537416	5.829283034134047E-4
78-79	0.0	0.006557943413400802	0.0	0.1838167250096936	5.829283034134047E-4
80-81	0.0	0.00656765888512436	0.0	0.18472997935170793	5.975015109987398E-4
82-83	0.0	0.006587089828571473	0.0	0.1852594725606418	6.606520772018587E-4
84-85	0.0	0.006616236243742143	0.0	0.18549264388200715	6.606520772018587E-4
86-87	0.0	0.006679386809945263	0.0	0.18563351822199872	6.65509813063637E-4
88	0.0	0.006985424169237299	0.0	0.18565294916544584	6.703675489254154E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	14070	0.0	51.577816	1
GTATCAA	26110	0.0	37.05342	1
TCAACGC	33475	0.0	28.465605	4
ATCAACG	34155	0.0	27.863302	3
TATCAAC	34450	0.0	27.789278	2
CAACGCA	34410	0.0	27.65682	5
AACGCAG	35005	0.0	27.313978	6
GTACATG	45610	0.0	23.031857	1
ACGCAGA	41670	0.0	22.906311	7
TACATGG	46030	0.0	22.46979	2
CGCAGAG	43050	0.0	22.162624	8
GTGGTAT	6580	0.0	21.786951	1
ACATGGG	47100	0.0	21.546623	3
TGGTATC	6930	0.0	19.459427	2
CATGGGG	35285	0.0	19.40763	4
GCAGAGT	49175	0.0	19.278627	9
GAGTACT	23400	0.0	18.154264	12-13
CAGAGTA	47085	0.0	17.528336	10-11
AGAGTAC	44250	0.0	17.118412	10-11
GTACTTT	25230	0.0	16.8476	14-15
>>END_MODULE