FastQCFastQC Report
Fri 27 May 2016
SRR2138728_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138728_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4515605
Sequences flagged as poor quality0
Sequence length19-100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA63600.054.0909161
GTATCAA123800.037.2998471
TCAACGC159550.028.5391354
ATCAACG161200.028.2968793
TATCAAC163250.028.1138692
CAACGCA162750.028.0767695
AACGCAG163950.027.8467566
GTGGTAT29700.023.5450971
ACGCAGA195100.023.4006967
CGCAGAG202250.022.5734278
GTACATG209900.022.5357341
TACATGG213600.021.9195862
TGGTATC31350.021.4085182
ACATGGG221450.020.8522053
GCAGAGT227450.019.8780739
CATGGGG165350.018.9096324
GAGTACT105650.018.86773912-13
CAGAGTA219200.017.89439210-11
AGAGTAC206900.017.25856410-11
GTACTTT115650.017.13412314-15
Produced by FastQC (version 0.11.5)