##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138728_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4515605
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.16272326742485	33.0	31.0	34.0	31.0	34.0
2	32.444960974221615	34.0	31.0	34.0	31.0	34.0
3	32.22438654399576	33.0	31.0	34.0	30.0	34.0
4	36.057443244039284	37.0	35.0	37.0	35.0	37.0
5	35.89981453205052	37.0	35.0	37.0	35.0	37.0
6	36.00133381905636	37.0	35.0	37.0	35.0	37.0
7	35.969648363840506	37.0	35.0	37.0	35.0	37.0
8	35.946695736230254	37.0	35.0	37.0	35.0	37.0
9	37.73078313980076	39.0	37.0	39.0	35.0	39.0
10-11	37.65350623006219	39.0	37.0	39.0	35.0	39.0
12-13	37.618491320653604	39.0	37.0	39.0	35.0	39.0
14-15	38.91555527996802	40.0	38.0	41.0	35.0	41.0
16-17	38.89646902242335	40.0	38.0	41.0	35.0	41.0
18-19	38.88181982259299	40.0	38.0	41.0	35.0	41.0
20-21	38.87871596730656	40.0	38.0	41.0	35.0	41.0
22-23	38.78860497506962	40.0	38.0	41.0	34.5	41.0
24-25	38.71476210116829	40.0	38.0	41.0	34.0	41.0
26-27	38.55865375210901	40.0	38.0	41.0	34.0	41.0
28-29	38.43713259262652	40.0	38.0	41.0	34.0	41.0
30-31	38.27931235999051	40.0	38.0	41.0	34.0	41.0
32-33	38.10459455986009	40.0	37.5	41.0	33.5	41.0
34-35	38.18606936991972	40.0	38.0	41.0	33.5	41.0
36-37	38.175228964155075	40.0	38.0	41.0	33.0	41.0
38-39	38.108697887652504	40.0	37.0	41.0	33.0	41.0
40-41	38.07506639226092	40.0	37.0	41.0	33.0	41.0
42-43	38.13823620744489	40.0	37.0	41.0	33.0	41.0
44-45	38.08329842911238	40.0	37.0	41.0	33.0	41.0
46-47	37.97435311246389	40.0	37.0	41.0	33.0	41.0
48-49	37.858448155902366	40.0	37.0	41.0	33.0	41.0
50-51	37.73055605998779	40.0	36.5	41.0	33.0	41.0
52-53	37.552817162184795	39.5	36.0	41.0	32.0	41.0
54-55	37.3633652319731	39.0	36.0	41.0	32.0	41.0
56-57	37.226444795532814	39.0	35.0	41.0	32.0	41.0
58-59	36.98847672326757	39.0	35.0	40.5	31.0	41.0
60-61	36.75458475936895	38.0	35.0	40.0	31.0	41.0
62-63	36.456688326645484	38.0	35.0	40.0	31.0	41.0
64-65	36.119357197217106	37.0	35.0	40.0	31.0	41.0
66-67	35.80838759853587	36.5	34.0	39.5	30.0	41.0
68-69	35.5086159299117	36.0	34.0	39.0	30.0	41.0
70-71	35.19941939268679	35.5	34.0	39.0	30.0	40.5
72-73	34.75180201785445	35.0	34.0	38.0	29.0	40.0
74-75	34.53051514691853	35.0	34.0	37.0	29.0	39.5
76-77	33.34843859322135	34.5	32.0	36.0	28.0	39.0
78-79	34.080640096421725	35.0	33.5	36.0	29.5	39.0
80-81	34.03748397145923	35.0	34.0	36.0	30.0	37.5
82-83	33.842989886785745	35.0	34.0	35.5	29.0	37.0
84-85	33.66405971586588	35.0	33.0	35.0	29.0	37.0
86-87	33.52245353951322	35.0	33.0	35.0	29.0	36.0
88-89	33.380679098586015	35.0	33.0	35.0	29.0	36.0
90-91	33.26012975910362	35.0	33.0	35.0	29.0	36.0
92-93	33.20105020371844	35.0	33.0	35.0	29.0	35.5
94-95	33.17686685831147	35.0	33.0	35.0	29.0	35.0
96-97	33.235694353426695	35.0	33.0	35.0	29.0	35.0
98-99	33.33634511474099	35.0	33.0	35.0	29.5	35.0
100	32.1530502151184	34.0	31.0	35.0	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	1.0
18	4.0
19	10.0
20	35.0
21	164.0
22	695.0
23	2297.0
24	6166.0
25	12983.0
26	22962.0
27	35923.0
28	51154.0
29	68052.0
30	87095.0
31	108677.0
32	136614.0
33	175322.0
34	241250.0
35	344057.0
36	556014.0
37	962710.0
38	1060845.0
39	615011.0
40	27563.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.738435934941165	19.119010630912136	13.174646586670002	23.967906847476694
2	16.130972483199926	20.811895637461646	39.32354579286718	23.73358608647125
3	19.06296055567305	25.678818231444072	29.489005349227842	25.769215863655038
4	13.437889275080526	16.903781442353793	36.77179469860627	32.8865345839594
5	13.933658944925432	37.66689070456783	34.22741803147087	14.172032319035877
6	31.68361714543234	35.800252679319826	18.162682519839535	14.3534476554083
7	28.308654100613317	31.891762011956317	21.927892275785858	17.871691611644508
8	26.438295643662368	34.86959111791222	20.360793293478945	18.33131994494647
9	27.166813749209684	15.64310873072379	20.296970173431912	36.893107346634615
10-11	25.840490919821374	25.760844892323398	27.436777574655004	20.961886613200225
12-13	26.347200430507094	23.4431266685195	27.774960387367802	22.434712513605596
14-15	23.607611826100822	24.42399191249013	25.52238293650574	26.446013324903305
16-17	23.06324401713613	27.4922407960838	26.16199822615131	23.28251696062875
18-19	23.040888208778227	26.716231822756864	27.961745989740024	22.28113397872489
20-21	24.006977334183944	25.777079443917273	27.81393657389725	22.402006648001528
22-23	23.905466504445467	25.5832952919749	27.61238923430664	22.898848969272994
24-25	23.340346780727955	25.86226357306991	27.713957683380237	23.083431962821898
26-27	23.243308179751526	25.991473027751173	27.762796304306363	23.002422488190938
28-29	23.223387048649478	25.985835146680948	27.78068879627364	23.010089008395934
30-31	23.54406549980931	25.92129809796271	27.462787205541478	23.071849196686507
32-33	23.2080275636335	26.21207371106075	27.44579234237686	23.13410638292889
34-35	23.39901328814097	26.243647064860497	27.517796957492784	22.839542689505752
36-37	23.43274088059252	26.129872678488653	27.420061737793706	23.017324703125123
38-39	23.258147977515222	26.320450260404154	27.490908229654853	22.93049353242578
40-41	23.53877674743254	26.10275651281253	27.336123857554558	23.022342882200377
42-43	23.29808470938628	26.181497895001037	27.12122707865446	23.399190316958222
44-45	23.285633051545222	26.33172065594216	26.998957769990994	23.383688522521627
46-47	23.546161442520468	26.241234659719463	26.753370262790554	23.459233634969515
48-49	23.419150844461555	26.41477444894276	26.5883485983936	23.577726108202082
50-51	23.40342304188908	26.511743358785054	26.574894524379012	23.509939074946853
52-53	23.64262253143745	26.460292680640197	26.331620614758506	23.565464173163853
54-55	23.56232480231161	26.238219367831444	26.49063533480149	23.708820495055456
56-57	23.48891411583005	26.42371509919447	26.451394778125554	23.63597600684992
58-59	23.4716440438962	26.54616907603444	26.432009365353252	23.550177514716104
60-61	23.657933565198476	26.450097406269258	26.479693850588248	23.412275177944014
62-63	23.69215862545326	26.528581700353314	26.354806265153783	23.424453409039632
64-65	23.602961993399145	26.505126027357218	26.349956721486116	23.541955257757518
66-67	23.710606351199427	26.49386383508831	26.160528076330046	23.63500173738222
68-69	23.494131767913824	26.471602607979232	26.370478589561113	23.663787034545827
70-71	23.519992543943143	26.28669388467361	26.424837734446527	23.768475836936716
72-73	23.6569524719878	26.393862393000322	26.163195708301956	23.785989426709918
74-75	23.39849942496212	26.520922251227656	26.36335822121616	23.717220102594062
76-77	23.616623399578664	26.24616108702386	26.290972810316365	23.84624270308111
78-79	23.403188169625906	26.274059525517046	26.3169987500919	24.00575355476514
80-81	23.720863728753088	26.386159105492318	26.104453121515935	23.78852404423866
82-83	23.64175608222791	26.229721879119122	26.420096025904606	23.70842601274836
84-85	23.668098510246395	26.294644173605775	26.181175828214283	23.856081487933544
86-87	23.45072176014264	26.451825461638794	26.41620189932127	23.681250878897306
88-89	23.580623912768512	26.204254144826216	26.40810748212382	23.807014460281454
90-91	23.648240014660885	26.10395513608015	26.419019984175502	23.82878486508347
92-93	23.734058167645173	26.163226136245278	26.361239933998824	23.741475762110724
94-95	23.60617913761622	26.139711832662414	26.456394652023274	23.797714377698096
96-97	23.48922616919627	26.190826986757955	26.566457581765196	23.753489262280578
98-99	24.690993358224432	28.24691370776982	26.675342000986852	20.386750933018895
100	32.40885742830978	39.81937806862616	27.771764503064073	0.0
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1397.0
1	1263.0
2	1739.5
3	2481.0
4	3218.0
5	5045.5
6	6733.5
7	8058.5
8	9084.5
9	9096.5
10	8086.0
11	6884.0
12	7125.0
13	9110.0
14	13843.0
15	20088.0
16	24873.0
17	27370.0
18	27350.5
19	25279.0
20	22278.0
21	19092.5
22	16977.5
23	17059.5
24	19114.5
25	23074.5
26	28874.0
27	35235.0
28	41903.0
29	50200.5
30	58083.0
31	65466.5
32	74627.5
33	84619.0
34	93531.5
35	103266.5
36	114459.5
37	124467.5
38	132327.5
39	137096.0
40	141349.0
41	145589.0
42	150333.5
43	157245.0
44	165056.0
45	172839.5
46	179081.5
47	185515.5
48	192992.0
49	199315.0
50	203819.0
51	207377.0
52	208548.5
53	207826.0
54	205816.0
55	201188.0
56	192765.0
57	179629.5
58	164469.0
59	147093.0
60	127931.0
61	108654.5
62	89533.5
63	71481.0
64	56272.0
65	45074.5
66	35265.5
67	25783.5
68	18739.0
69	13833.5
70	9513.5
71	6227.5
72	4167.0
73	2723.5
74	1740.5
75	1145.5
76	779.0
77	516.5
78	342.0
79	215.5
80	148.0
81	112.0
82	87.0
83	58.0
84	34.5
85	18.0
86	8.5
87	6.5
88	5.0
89	4.5
90	3.5
91	2.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.00529296154596998
22-23	0.004163887972808925
24-25	0.003090325906434679
26-27	0.014870285568210564
28-29	0.019628883247402445
30-31	0.03751245739152639
32-33	0.021776799512949
34-35	0.011348340851282402
36-37	0.006405703421795947
38-39	0.0029377015969254787
40-41	0.0074709765798122475
42-43	0.0090096361201201
44-45	0.006509091620888807
46-47	0.0020585536434544398
48-49	0.010176345873352097
50-51	0.0045308200414914766
52-53	2.4934024570985175E-5
54-55	5.807275708493948E-4
56-57	8.31791107308475E-4
58-59	4.282917596146309E-4
60-61	0.001342881130400472
62-63	8.944342029503245E-4
64-65	0.0
66-67	1.7847434637202842E-4
68-69	6.970176010884626E-4
70-71	2.6911986896411937E-4
72-73	5.46702806599566E-4
74-75	3.6510208254227885E-4
76-77	0.0
78-79	0.0
80-81	3.065010559727631E-4
82-83	0.001294090884782411
84-85	0.0
86-87	7.901950023875176E-4
88-89	3.6138253129408456E-4
90-91	0.007846244457122873
92-93	8.051365657225269E-4
94-95	5.260509182218777E-5
96-97	3.0694580438488334E-4
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	113.0
20-21	338.0
22-23	713.0
24-25	1564.0
26-27	2873.0
28-29	6702.0
30-31	14808.0
32-33	19356.0
34-35	26318.0
36-37	39531.0
38-39	47911.0
40-41	47658.0
42-43	52474.0
44-45	61852.0
46-47	61227.0
48-49	57832.0
50-51	51712.0
52-53	49051.0
54-55	52823.0
56-57	54542.0
58-59	54767.0
60-61	53358.0
62-63	50546.0
64-65	51890.0
66-67	54935.0
68-69	56454.0
70-71	55963.0
72-73	51701.0
74-75	51892.0
76-77	52166.0
78-79	55875.0
80-81	56353.0
82-83	53397.0
84-85	52965.0
86-87	55382.0
88-89	60245.0
90-91	63440.0
92-93	65970.0
94-95	73299.0
96-97	160057.0
98-99	906969.0
100-101	1728583.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.83852140494491
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.87556941942023	78.00624510795461
2	6.357740231106949	10.914780418299065
3	1.5160400066746014	3.9040389769107184
4	0.5667749980113342	1.946045111943341
5	0.24666546722751376	1.0586699494234837
6	0.12956284848801694	0.6672890005934567
7	0.08713114142924182	0.5235445844027878
8	0.052881979571506095	0.36314487483076685
9	0.036364647590170864	0.2809338822496944
>10	0.12540359221826425	1.7546393970425382
>50	0.0039988315806570906	0.23867953420956023
>100	0.001782496815067791	0.3038002224334927
>500	8.433986635950854E-5	0.03818893970653676
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	6.643628040982326E-5	0.0	0.0	0.0
3	0.0	6.643628040982326E-5	0.0	0.0	0.0
4	0.0	1.5501798762292097E-4	0.0	0.0	0.0
5	0.0	1.7716341442619538E-4	0.0	0.0	0.0
6	2.2145426803274423E-5	1.7716341442619538E-4	0.0	0.0	0.0
7	2.2145426803274423E-5	1.7716341442619538E-4	0.0	0.0	0.0
8	2.2145426803274423E-5	1.7716341442619538E-4	0.0	0.0	4.4290853606548846E-5
9	2.2145426803274423E-5	3.986176824589396E-4	0.0	0.0	8.858170721309769E-5
10-11	2.2145426803274423E-5	3.986176824589396E-4	0.0	0.0	8.858170721309769E-5
12-13	2.2145426803274423E-5	4.0969039586057684E-4	0.0	0.0	8.858170721309769E-5
14-15	2.2145426803274423E-5	5.868538102867722E-4	0.0	0.0	1.2179984741800932E-4
16-17	4.4290853606548846E-5	5.979265236884094E-4	0.0	0.0	1.5501798762292097E-4
18-19	4.4290853606548846E-5	6.975809443031443E-4	0.0	0.0	1.5501798762292097E-4
20-21	4.4290853606548846E-5	8.41526218524428E-4	0.0	0.0	1.5501798762292097E-4
22-23	4.4290853606548846E-5	9.854714927457118E-4	0.0	2.2145426803274423E-5	1.993088412294698E-4
24-25	4.4290853606548846E-5	0.0010851259133604467	0.0	3.321814020491164E-5	2.2145426803274422E-4
26-27	4.4290853606548846E-5	0.0011072713401637211	0.0	1.3287256081964655E-4	2.2145426803274422E-4
28-29	4.4290853606548846E-5	0.0011183440535653584	0.0	4.650539628687629E-4	2.2145426803274422E-4
30-31	4.4290853606548846E-5	0.0011847803339751816	0.0	0.001993088412294698	2.2145426803274422E-4
32-33	4.4290853606548846E-5	0.001206925760778456	0.0	0.004694830482294177	2.878905484425675E-4
34-35	4.4290853606548846E-5	0.0014505254556144748	0.0	0.008603498313072113	3.3218140204911635E-4
36-37	4.4290853606548846E-5	0.0016498342968439446	0.0	0.014206291294300542	3.65399542254028E-4
38-39	4.4290853606548846E-5	0.001782706857663591	0.0	0.023806333813520007	3.764722556556652E-4
40-41	4.4290853606548846E-5	0.0020152338390979725	0.0	0.03319599477810836	3.764722556556652E-4
42-43	4.4290853606548846E-5	0.002048451979302884	0.0	0.04213167449322959	3.764722556556652E-4
44-45	4.4290853606548846E-5	0.002314197100942177	0.0	0.05230749810933419	3.764722556556652E-4
46-47	4.4290853606548846E-5	0.0025467240823765585	0.0	0.06340235693777467	3.764722556556652E-4
48-49	4.4290853606548846E-5	0.0025467240823765585	0.0	0.07379963482191201	4.20763109262214E-4
50-51	4.4290853606548846E-5	0.0028124692040158517	0.0	0.08357684075555767	4.318358226638512E-4
52-53	4.4290853606548846E-5	0.002834614630819126	0.0	0.093730518944859	4.5398124946712567E-4
54-55	4.4290853606548846E-5	0.0028456873442207634	0.0	0.10323090704346372	4.7612667627040014E-4
56-57	4.4290853606548846E-5	0.002878905484425675	0.0	0.11235482288641278	4.871993896720373E-4
58-59	4.4290853606548846E-5	0.0029010509112289496	0.0	0.12246421022210756	4.982721030736745E-4
60-61	5.5363567008186054E-5	0.003033923472048596	0.0	0.13249608856399087	5.093448164753117E-4
62-63	6.643628040982326E-5	0.003144650606064968	0.0	0.1425611850460791	5.314902432785861E-4
64-65	6.643628040982326E-5	0.0034768320081140845	0.0	0.15131970134677414	5.314902432785861E-4
66-67	6.643628040982326E-5	0.003521122861720633	0.0	0.1606539987443543	5.425629566802233E-4
68-69	6.643628040982326E-5	0.0035543410019255447	0.0	0.16971147830689354	5.536356700818606E-4
70-71	7.750899381146048E-5	0.003576486428728819	0.0	0.1774070141210314	5.75781096885135E-4
72-73	1.3287256081964653E-4	0.0035986318555320937	0.0	0.18305409795586636	6.643628040982327E-4
74-75	1.4394527422128373E-4	0.003764722556556652	0.0	0.1875496195969311	6.643628040982327E-4
76-77	1.5501798762292097E-4	0.0038754496905730237	0.0	0.19194548681738105	6.643628040982327E-4
78-79	1.5501798762292097E-4	0.003919740544179573	0.0	0.19511228285024929	6.643628040982327E-4
80-81	1.5501798762292097E-4	0.00393081325758121	0.0	0.1960645362027901	6.754355174998698E-4
82-83	1.5501798762292097E-4	0.003952958684384484	0.0	0.19661817187287195	7.086536577047815E-4
84-85	1.5501798762292097E-4	0.003986176824589396	0.0	0.19683962614090472	7.086536577047815E-4
86-87	1.5501798762292097E-4	0.004074758531802494	0.0	0.19695035327492108	7.086536577047815E-4
88	1.5501798762292097E-4	0.004296212799835238	0.0	0.19696142598832272	7.307990845080559E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6360	0.0	54.090916	1
GTATCAA	12380	0.0	37.299847	1
TCAACGC	15955	0.0	28.539135	4
ATCAACG	16120	0.0	28.296879	3
TATCAAC	16325	0.0	28.113869	2
CAACGCA	16275	0.0	28.076769	5
AACGCAG	16395	0.0	27.846756	6
GTGGTAT	2970	0.0	23.545097	1
ACGCAGA	19510	0.0	23.400696	7
CGCAGAG	20225	0.0	22.573427	8
GTACATG	20990	0.0	22.535734	1
TACATGG	21360	0.0	21.919586	2
TGGTATC	3135	0.0	21.408518	2
ACATGGG	22145	0.0	20.852205	3
GCAGAGT	22745	0.0	19.878073	9
CATGGGG	16535	0.0	18.909632	4
GAGTACT	10565	0.0	18.867739	12-13
CAGAGTA	21920	0.0	17.894392	10-11
AGAGTAC	20690	0.0	17.258564	10-11
GTACTTT	11565	0.0	17.134123	14-15
>>END_MODULE