FastQCFastQC Report
Fri 27 May 2016
SRR2138727_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138727_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8888940
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA113850.049.5334171
CTTATAC105300.042.0012551
TATACAC123700.037.2274133
GTATCAA209500.035.128071
TTATACA135750.033.1346552
TACACAT171650.026.9434665
TCAACGC269300.026.812574
TATCAAC272900.026.6331332
ATCAACG272400.026.5219823
CAACGCA272900.026.5024365
AACGCAG280100.025.8777836
ACACATC182700.024.2293116
ATACACA202050.023.9095464
ACATCTC188700.023.5219088
CACATCT197250.022.221057
GTACATG342350.021.9711251
ACGCAGA331050.021.895087
TACATGG345350.021.4933822
CGCAGAG339550.021.2886228
ACATGGG351850.020.9161033
Produced by FastQC (version 0.11.5)