FastQCFastQC Report
Fri 27 May 2016
SRR2138726_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138726_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12136625
Sequences flagged as poor quality0
Sequence length19-100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA156100.052.4554631
GTATCAA282350.035.379491
TCAACGC351400.027.941494
TATCAAC361750.027.3668372
ATCAACG360550.027.2323933
CAACGCA364900.026.8966125
AACGCAG369400.026.6129866
GTGGTAT72100.023.0633411
GTACATG477200.022.6969761
TGGTATC69100.022.4171872
ACGCAGA439150.022.3860577
TACATGG483100.021.956912
CGCAGAG466000.021.0613178
ACATGGG495200.020.9934733
CGTCGAA11150.019.22829894
CATGGGG370650.019.0861994
GAGTACT254800.018.64504612-13
GCAGAGT528200.018.4580149
CAGAGTA504600.017.58092310-11
GTACTTT282550.016.86589614-15
Produced by FastQC (version 0.11.5)