##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138726_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	12136625
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.14615743668442	33.0	31.0	34.0	31.0	34.0
2	32.43075039395219	34.0	31.0	34.0	31.0	34.0
3	32.2100134922188	33.0	31.0	34.0	30.0	34.0
4	36.049778171443876	37.0	35.0	37.0	35.0	37.0
5	35.88699148239317	37.0	35.0	37.0	35.0	37.0
6	35.988444645855004	37.0	35.0	37.0	35.0	37.0
7	35.94972416137106	37.0	35.0	37.0	35.0	37.0
8	35.92630471815682	37.0	35.0	37.0	35.0	37.0
9	37.71128629252366	39.0	37.0	39.0	35.0	39.0
10-11	37.62932569804208	39.0	37.0	39.0	35.0	39.0
12-13	37.599752855509664	39.0	37.0	39.0	35.0	39.0
14-15	38.906394858537695	40.0	38.0	41.0	35.0	41.0
16-17	38.88273432688247	40.0	38.0	41.0	35.0	41.0
18-19	38.85489475039395	40.0	38.0	41.0	35.0	41.0
20-21	38.84736912899838	40.0	38.0	41.0	35.0	41.0
22-23	38.758416902826156	40.0	38.0	41.0	34.5	41.0
24-25	38.689506024622894	40.0	38.0	41.0	34.0	41.0
26-27	38.53650115525657	40.0	38.0	41.0	34.0	41.0
28-29	38.40740044689176	40.0	38.0	41.0	34.0	41.0
30-31	38.25326147310865	40.0	38.0	41.0	34.0	41.0
32-33	38.08028528645623	40.0	37.5	41.0	33.0	41.0
34-35	38.1684662295543	40.0	38.0	41.0	33.0	41.0
36-37	38.15851235291762	40.0	38.0	41.0	33.0	41.0
38-39	38.08672384293249	40.0	37.0	41.0	33.0	41.0
40-41	38.04780127834377	40.0	37.0	41.0	33.0	41.0
42-43	38.11018751069936	40.0	37.0	41.0	33.0	41.0
44-45	38.055788575583996	40.0	37.0	41.0	33.0	41.0
46-47	37.94799928025013	40.0	37.0	41.0	33.0	41.0
48-49	37.82886453390611	40.0	37.0	41.0	33.0	41.0
50-51	37.694649522951515	40.0	36.5	41.0	33.0	41.0
52-53	37.510455060957	39.5	36.0	41.0	32.0	41.0
54-55	37.31740901405789	39.0	36.0	41.0	32.0	41.0
56-57	37.18466543575158	39.0	35.0	41.0	32.0	41.0
58-59	36.9377486624803	39.0	35.0	40.5	31.0	41.0
60-61	36.70183724242797	38.0	35.0	40.0	31.0	41.0
62-63	36.40434964735683	37.5	35.0	40.0	31.0	41.0
64-65	36.06466762621354	37.0	35.0	40.0	30.5	41.0
66-67	35.752632258329854	36.5	34.0	39.5	30.0	41.0
68-69	35.45474208763471	36.0	34.0	39.0	30.0	41.0
70-71	35.14717233326438	35.5	34.0	39.0	30.0	40.0
72-73	34.69599957220753	35.0	34.0	38.0	29.0	40.0
74-75	34.4744454803217	35.0	34.0	37.0	29.0	39.5
76-77	33.301535053654774	34.5	32.0	36.0	28.0	39.0
78-79	34.03132801309827	35.0	33.0	36.0	29.0	39.0
80-81	33.987850794461025	35.0	34.0	36.0	29.0	37.5
82-83	33.79139222169346	35.0	33.0	35.5	29.0	37.0
84-85	33.61140292844793	35.0	33.0	35.0	29.0	37.0
86-87	33.46450487263806	35.0	33.0	35.0	29.0	36.0
88-89	33.32469560478409	35.0	33.0	35.0	29.0	36.0
90-91	33.20409205455867	35.0	33.0	35.0	29.0	36.0
92-93	33.14297313509313	35.0	33.0	35.0	29.0	35.5
94-95	33.12066583745558	35.0	33.0	35.0	29.0	35.0
96-97	33.187295878465235	35.0	33.0	35.0	29.0	35.0
98-99	33.291732548739255	35.0	33.0	35.0	29.5	35.0
100	32.09343509135729	34.0	31.0	35.0	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	1.0
18	3.0
19	14.0
20	77.0
21	384.0
22	1846.0
23	6490.0
24	16698.0
25	35551.0
26	63890.0
27	101053.0
28	144572.0
29	191543.0
30	244058.0
31	302762.0
32	376685.0
33	482207.0
34	655184.0
35	939858.0
36	1525390.0
37	2586938.0
38	2824559.0
39	1566910.0
40	69951.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.59081705169271	19.150950119988053	13.196617675836569	24.06161515248267
2	16.364574171155493	20.645162885068956	39.127516916770524	23.862746027005034
3	19.20547928274953	24.97304646060993	29.45139196440526	26.37008229223528
4	13.517744842573615	16.798162586386248	36.62462999392335	33.05946257711678
5	14.248433975672809	37.16813364506195	34.36892051950192	14.21451185976332
6	32.37707352744276	35.70944970286221	17.73668544591268	14.176791323782354
7	28.662482362271223	31.598265580422897	21.657050456778553	18.082201600527327
8	26.339390069314987	35.13109286972284	20.036278619467932	18.493238441494235
9	26.69798234682212	15.746890095063495	20.2731978618438	37.28192969627059
10-11	25.808373415179258	25.73156046264921	27.474656257402696	20.98540986476883
12-13	26.3227956701307	23.312201703521364	27.697980286941387	22.667022339406547
14-15	23.451684467469335	24.46369975178437	25.35178025192341	26.73283552882288
16-17	23.25132810810254	27.55266394075783	25.94453153162432	23.25147641951531
18-19	23.221641106979906	26.70211446757233	27.614266733956107	22.461977691491665
20-21	24.194856659437082	25.800271293697985	27.491569916880206	22.513302129984726
22-23	23.981882385575727	25.64230522221203	27.292715185507227	23.083097206705016
24-25	23.51601283332963	25.882901369093737	27.453396300124705	23.147689497451925
26-27	23.522817449068057	25.992085099101196	27.393659626274008	23.09143782555674
28-29	23.520855976031946	25.968271092092905	27.416787502241487	23.09408542963366
30-31	23.7440298240808	25.87834290149557	27.14332872129386	23.23429855312977
32-33	23.445537175363018	26.117539405087815	27.170012325635245	23.266911093913922
34-35	23.652323090183625	26.14204372265303	27.170807617118122	23.034825570045232
36-37	23.71116913363019	25.971535416927193	27.03695484266193	23.280340606780683
38-39	23.395661489126343	26.330712974312647	27.153906382468772	23.119719154092234
40-41	23.75768384270308	26.060659007449814	27.037360031204237	23.144297118642864
42-43	23.456896890057934	26.168862532900434	26.856686132147633	23.517554444894
44-45	23.464230467504954	26.262964190380234	26.76538111415522	23.507424227959593
46-47	23.78235897842859	26.144682642787632	26.591307488545425	23.48165089023836
48-49	23.60585749096062	26.41051164928546	26.32080113152832	23.6628297282256
50-51	23.624988829044423	26.416635048754433	26.394867871050533	23.563508251150616
52-53	23.896079778520825	26.405137855239662	26.156172259988647	23.542610106250866
54-55	23.82719689024592	26.179420556988305	26.3288057106339	23.664576842131872
56-57	23.80948504919066	26.38967573672129	26.18320571538454	23.617633498703512
58-59	23.6642807604042	26.455993164460534	26.251151934048178	23.628574141087093
60-61	23.786567983025154	26.407845774306065	26.32681409514826	23.478772147520523
62-63	23.942110904131358	26.42268602370518	26.15123527028845	23.483967801875018
64-65	23.77994770099809	26.46878210014426	26.183414493071776	23.567855705785874
66-67	23.97781160291958	26.379980035878454	26.00313793547154	23.63907042573043
68-69	23.748217689067342	26.333051621419866	26.229967435323214	23.688763254189574
70-71	23.759296829153474	26.15295642584914	26.250270524734148	23.837476220263238
72-73	23.904773829420872	26.223352096532572	26.035580576177647	23.83629349786891
74-75	23.62515291694948	26.37557898417004	26.200744778336322	23.798523320544156
76-77	23.862767223310527	26.246377050091922	26.082625683704574	23.808230042892973
78-79	23.627909549995714	26.246905569876013	26.141788245061186	23.983396635067088
80-81	23.917471750531085	26.255660949404707	26.015490727187263	23.81137657287694
82-83	23.840801673579495	26.176417542863405	26.28490592419852	23.697874859358574
84-85	23.880938150829607	26.20165303540113	26.10135822650338	23.816050587265885
86-87	23.638568328871585	26.335865451658652	26.229518572351644	23.79604764711812
88-89	23.808690938994772	26.091724917395194	26.218575233785575	23.88100890982446
90-91	23.733189263888104	26.109287463760616	26.254982567223827	23.902540705127453
92-93	23.83701699546853	26.152299401374187	26.27299524244837	23.737688360708916
94-95	23.74972245157153	26.188122403332276	26.304480754391545	23.757674390704647
96-97	23.614174784381795	26.278144592564512	26.42988974913437	23.677790873919324
98-99	24.955728329300094	28.286576385339256	26.461064771934527	20.29663051342612
100	32.58691773680633	39.9271279109794	27.48595435221427	0.0
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3366.0
1	2880.0
2	3437.0
3	4778.0
4	6206.5
5	9707.0
6	12911.5
7	15775.0
8	18381.0
9	18771.5
10	16758.0
11	14248.5
12	14860.0
13	19316.0
14	30265.0
15	45132.5
16	56885.5
17	63229.0
18	64144.5
19	60520.0
20	54402.5
21	48123.0
22	44284.5
23	45154.5
24	50889.0
25	61833.5
26	78023.5
27	95774.0
28	113342.0
29	134798.0
30	155606.0
31	173684.0
32	195043.5
33	218501.5
34	239658.0
35	262255.0
36	290423.5
37	315065.0
38	334352.0
39	350066.0
40	365931.0
41	383124.5
42	399192.0
43	418698.5
44	441452.5
45	465152.5
46	488605.5
47	511446.0
48	534101.5
49	551617.5
50	557610.5
51	561958.5
52	559732.5
53	549850.5
54	539394.0
55	522439.5
56	498067.0
57	465396.0
58	428646.5
59	385533.5
60	338130.5
61	291830.5
62	245483.0
63	200402.5
64	162020.5
65	134063.0
66	109651.0
67	80216.5
68	56120.5
69	42588.5
70	29398.0
71	18936.5
72	12961.5
73	8692.0
74	5872.0
75	4021.0
76	2693.5
77	1733.5
78	1132.0
79	734.0
80	468.0
81	300.5
82	221.0
83	152.5
84	100.0
85	66.5
86	45.5
87	32.5
88	19.0
89	11.5
90	8.5
91	5.5
92	3.5
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.005079855074577464
22-23	0.004919663623562173
24-25	0.0037255442643082436
26-27	0.016808349606415817
28-29	0.021715765835798276
30-31	0.03964070613123781
32-33	0.024019862281698605
34-35	0.012374337457348637
36-37	0.0072334204656486125
38-39	0.0027970063146331666
40-41	0.007850877266086062
42-43	0.00954092150882464
44-45	0.006103378562994687
46-47	0.001997181892211606
48-49	0.0094099676551994
50-51	0.0043932239858217445
52-53	9.188786372662258E-6
54-55	4.972354868695211E-4
56-57	7.104516948366253E-4
58-59	4.5276474596394787E-4
60-61	0.0010185577357625661
62-63	8.993371884920814E-4
64-65	0.0
66-67	2.1562729213692012E-4
68-69	6.610524667172796E-4
70-71	2.3215417926533005E-4
72-73	6.801942655751537E-4
74-75	3.3943125111707354E-4
76-77	0.0
78-79	5.456105007760491E-6
80-81	4.100815330754031E-4
82-83	0.0021671313052474242
84-85	1.714636283921364E-5
86-87	6.619417958062851E-4
88-89	2.776227389326138E-4
90-91	0.00880788338675458
92-93	8.047962910072123E-4
94-95	6.909263035392511E-5
96-97	2.9736118452664266E-4
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	272.0
20-21	976.0
22-23	2046.0
24-25	4041.0
26-27	7734.0
28-29	17606.0
30-31	38110.0
32-33	49998.0
34-35	68140.0
36-37	102168.0
38-39	116842.0
40-41	116367.0
42-43	130432.0
44-45	152149.0
46-47	151446.0
48-49	140806.0
50-51	124861.0
52-53	121023.0
54-55	130084.0
56-57	136960.0
58-59	133989.0
60-61	129966.0
62-63	124899.0
64-65	130622.0
66-67	137209.0
68-69	140875.0
70-71	138983.0
72-73	128418.0
74-75	130088.0
76-77	130558.0
78-79	141001.0
80-81	141477.0
82-83	134708.0
84-85	135292.0
86-87	143918.0
88-89	155712.0
90-91	165807.0
92-93	173210.0
94-95	199990.0
96-97	448221.0
98-99	2531435.0
100-101	4828186.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.52267849369377
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.46910061931268	74.72698926146792
2	7.196347645084414	12.021164613784716
3	1.7582611463376185	4.405640413005312
4	0.6788533258168337	2.267985923062966
5	0.321116461083707	1.3410253469063595
6	0.18526409768677493	0.9284252200510068
7	0.10411816248496521	0.6087359467410143
8	0.06221954652676461	0.4157394544462706
9	0.04648377815402594	0.34942046921378056
>10	0.169889768803459	2.2199116136428003
>50	0.005931924763296536	0.3386770890814299
>100	0.002355528664136335	0.3446545585886454
>500	5.79952814877652E-5	0.03163009000769871
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.6479045863244517E-5
2	0.0	9.063475224784485E-5	0.0	0.0	3.295809172648903E-5
3	0.0	9.063475224784485E-5	0.0	0.0	3.295809172648903E-5
4	0.0	2.2246711915380099E-4	0.0	0.0	4.119761465811129E-5
5	0.0	2.3070664208542325E-4	0.0	0.0	4.119761465811129E-5
6	1.6479045863244517E-5	2.3070664208542325E-4	0.0	0.0	5.767666052135581E-5
7	1.6479045863244517E-5	2.471856879486678E-4	0.0	8.239522931622258E-6	5.767666052135581E-5
8	1.6479045863244517E-5	2.8838330260677906E-4	0.0	8.239522931622258E-6	6.591618345297807E-5
9	2.4718568794866776E-5	5.932456510768027E-4	0.0	1.6479045863244517E-5	9.063475224784485E-5
10-11	4.119761465811129E-5	5.932456510768027E-4	0.0	2.0598807329055647E-5	9.063475224784485E-5
12-13	4.119761465811129E-5	6.097246969400472E-4	0.0	2.4718568794866776E-5	9.063475224784485E-5
14-15	4.943713758973355E-5	8.28072054628037E-4	0.0	2.4718568794866776E-5	1.6067069716663407E-4
16-17	4.943713758973355E-5	8.527906234229038E-4	0.0	2.4718568794866776E-5	2.0598807329055648E-4
18-19	5.355689905554468E-5	8.898684766152039E-4	0.0	4.943713758973355E-5	2.3070664208542325E-4
20-21	7.415570638460033E-5	9.475451371365599E-4	0.0	8.651499078203372E-5	2.3070664208542325E-4
22-23	9.063475224784485E-5	0.00102170084352116	0.0	1.3595212837176727E-4	2.677844952777234E-4
24-25	1.235928439743339E-4	0.0011411739260296827	0.0	1.5655093570082293E-4	3.2546115579907924E-4
26-27	1.4831141276920067E-4	0.0012029703480168498	0.0	2.3894616501704552E-4	3.2958091726489034E-4
28-29	1.4831141276920067E-4	0.001215329632414283	0.0	7.703953941066812E-4	3.2958091726489034E-4
30-31	1.4831141276920067E-4	0.0014254374671706508	0.0	0.0022287909530038213	3.2958091726489034E-4
32-33	1.524311742350118E-4	0.0014460362744997065	0.0	0.004832480199396455	3.666587704571905E-4
34-35	1.5655093570082293E-4	0.0017220602927090522	0.0	0.008396073867323082	3.954971007178684E-4
36-37	1.5655093570082293E-4	0.0019774855035893423	0.0	0.013578733791313483	4.160959080469241E-4
38-39	1.5655093570082293E-4	0.002072240017302998	0.0	0.021727621970687897	4.572935227050354E-4
40-41	1.6479045863244517E-4	0.002323545466717477	0.0	0.030300845581040858	4.7377256856827993E-4
42-43	1.6479045863244517E-4	0.0024265395033627552	0.0	0.03821490735686404	5.149701832263912E-4
44-45	1.6479045863244517E-4	0.0028220366040806236	0.0	0.04696528071024687	5.561677978845025E-4
46-47	1.6479045863244517E-4	0.003081581576426725	0.0	0.05667967824662952	5.973654125426138E-4
48-49	1.6479045863244517E-4	0.0030857013378925357	0.0	0.0665959440948369	6.262037428032917E-4
50-51	1.689102200982563E-4	0.00346471939274716	0.0	0.07579125168652734	6.262037428032917E-4
52-53	1.7302998156406744E-4	0.0034894379615420267	0.0	0.08493300237916224	6.921199262562698E-4
54-55	1.7302998156406744E-4	0.003522396053268516	0.0	0.0940253159342074	7.580361097092479E-4
56-57	1.7302998156406744E-4	0.003592431998187305	0.0	0.10314234805804745	7.786349170383034E-4
58-59	1.7714974302987857E-4	0.0036171505669821717	0.0	0.11243241016345154	7.909942014357368E-4
60-61	1.812695044956897E-4	0.0036995457962983947	0.0	0.12162359799367617	7.992337243673592E-4
62-63	1.853892659615008E-4	0.003744863172422317	0.0	0.12983016283357193	8.404313390254704E-4
64-65	1.8950902742731195E-4	0.00423099502538803	0.0	0.13792961387535663	8.445511004912816E-4
66-67	1.9362878889312308E-4	0.00426807287858033	0.0	0.1464328015407908	8.569103848887149E-4
68-69	2.0598807329055648E-4	0.004366947153759798	0.0	0.1546146478118917	8.651499078203372E-4
70-71	2.0598807329055648E-4	0.004420504052815342	0.0	0.16156880516618088	9.22826568341693E-4
72-73	2.0598807329055648E-4	0.004436983098678586	0.0	0.1667926627048294	0.0010299403664527824
74-75	2.0598807329055648E-4	0.004502899282131564	0.0	0.17101129844581997	0.0010299403664527824
76-77	2.0598807329055648E-4	0.004692408309558876	0.0	0.17529997013172938	0.0010464194123160268
78-79	2.0598807329055648E-4	0.004762444254477666	0.0	0.17813848578167324	0.0010587786967134602
80-81	2.0598807329055648E-4	0.004766564015943477	0.0	0.1790077554509594	0.0010958565499057603
82-83	2.0598807329055648E-4	0.004795402346204155	0.0	0.17974107299187378	0.0011658924948245496
84-85	2.0598807329055648E-4	0.0048530790067255105	0.0	0.17997589939542502	0.001174132017756172
86-87	2.0598807329055648E-4	0.004914875428712677	0.0	0.18009949223939936	0.0011823715406877942
88	2.0598807329055648E-4	0.005116743740537423	0.0	0.18010773176233097	0.0011947308250852275
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	15610	0.0	52.455463	1
GTATCAA	28235	0.0	35.37949	1
TCAACGC	35140	0.0	27.94149	4
TATCAAC	36175	0.0	27.366837	2
ATCAACG	36055	0.0	27.232393	3
CAACGCA	36490	0.0	26.896612	5
AACGCAG	36940	0.0	26.612986	6
GTGGTAT	7210	0.0	23.063341	1
GTACATG	47720	0.0	22.696976	1
TGGTATC	6910	0.0	22.417187	2
ACGCAGA	43915	0.0	22.386057	7
TACATGG	48310	0.0	21.95691	2
CGCAGAG	46600	0.0	21.061317	8
ACATGGG	49520	0.0	20.993473	3
CGTCGAA	1115	0.0	19.228298	94
CATGGGG	37065	0.0	19.086199	4
GAGTACT	25480	0.0	18.645046	12-13
GCAGAGT	52820	0.0	18.458014	9
CAGAGTA	50460	0.0	17.580923	10-11
GTACTTT	28255	0.0	16.865896	14-15
>>END_MODULE