FastQCFastQC Report
Fri 27 May 2016
SRR2138725_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138725_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14751916
Sequences flagged as poor quality0
Sequence length19-100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA180600.050.534941
GTATCAA320550.034.8522221
TCAACGC400000.027.4426364
ATCAACG409700.026.8227183
TATCAAC414300.026.740392
CAACGCA417800.026.2540425
AACGCAG423750.025.9811676
GTACATG539150.022.7986811
TACATGG539650.022.3714852
ACGCAGA504500.021.8065477
ACATGGG557600.021.2728713
CGCAGAG518400.021.23758
GTGGTAT87150.020.2088851
CATGGGG417500.019.507854
TGGTATC90400.018.4947662
GCAGAGT602350.018.1832929
GAGTACT309000.017.77556612-13
CAGAGTA573100.017.31278810-11
AGAGTAC535800.016.70409610-11
CGTCGAA11400.016.18975894
Produced by FastQC (version 0.11.5)