##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138725_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	14751916
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.7641523311277	31.0	31.0	34.0	30.0	34.0
2	32.03202621273061	33.0	31.0	34.0	30.0	34.0
3	31.937879662546884	31.0	31.0	34.0	30.0	34.0
4	35.79371832106419	37.0	35.0	37.0	35.0	37.0
5	35.57702145267096	37.0	35.0	37.0	33.0	37.0
6	35.66026013163307	37.0	35.0	37.0	33.0	37.0
7	35.578549864302374	37.0	35.0	37.0	33.0	37.0
8	35.56386668687647	37.0	35.0	37.0	33.0	37.0
9	37.28655003187382	39.0	37.0	39.0	34.0	39.0
10-11	37.158541236270594	39.0	37.0	39.0	33.0	39.0
12-13	37.11594348151115	39.0	37.0	39.0	33.0	39.0
14-15	38.297695024836095	40.0	38.0	41.0	33.0	41.0
16-17	38.251289222362715	40.0	38.0	41.0	33.0	41.0
18-19	38.252925382709606	40.0	38.0	41.0	33.0	41.0
20-21	38.251417022682226	40.0	38.0	41.0	33.0	41.0
22-23	38.18269097269617	40.0	37.5	41.0	33.0	41.0
24-25	38.1003129326884	40.0	37.0	41.0	33.0	41.0
26-27	37.92909734453541	40.0	37.0	41.0	33.0	41.0
28-29	37.777635659450496	40.0	37.0	41.0	32.0	41.0
30-31	37.61888008481128	40.0	37.0	41.0	32.0	41.0
32-33	37.451157552278445	39.0	36.0	41.0	32.0	41.0
34-35	37.32159455754676	39.0	36.0	41.0	31.0	41.0
36-37	37.410260195480454	39.0	36.0	41.0	31.0	41.0
38-39	37.37727479678867	39.0	36.0	41.0	31.0	41.0
40-41	37.34177678847519	39.0	36.0	41.0	31.0	41.0
42-43	37.41979264869114	39.0	36.0	41.0	31.0	41.0
44-45	37.451357859146356	40.0	36.0	41.0	31.5	41.0
46-47	37.32939835995873	39.0	36.0	41.0	31.0	41.0
48-49	37.25776255311111	39.0	36.0	41.0	31.0	41.0
50-51	37.15645356843774	39.0	36.0	41.0	31.0	41.0
52-53	36.99925580393816	39.0	35.0	41.0	31.0	41.0
54-55	36.81619484808611	39.0	35.0	40.0	31.0	41.0
56-57	36.67061083581157	38.5	35.0	40.0	30.0	41.0
58-59	36.399525242593384	38.0	35.0	40.0	30.0	41.0
60-61	36.232830462547454	38.0	34.5	40.0	30.0	41.0
62-63	35.96183681849628	37.5	34.0	40.0	29.0	41.0
64-65	35.650672747910306	37.0	34.0	40.0	29.0	41.0
66-67	35.31596864961942	36.5	34.0	39.0	29.0	41.0
68-69	35.077794860693544	36.0	34.0	39.0	29.0	41.0
70-71	34.75653433782739	35.5	33.0	39.0	28.0	40.0
72-73	34.46391975094261	35.0	33.0	38.0	28.0	40.0
74-75	34.183256267661335	35.0	33.0	37.0	28.0	39.5
76-77	32.841938457974564	34.0	31.5	36.0	26.5	39.0
78-79	33.59346741652308	35.0	32.5	36.0	28.0	39.0
80-81	33.63142039302726	35.0	33.0	36.0	29.0	38.5
82-83	33.40939344170181	35.0	33.0	36.0	28.5	37.0
84-85	33.2149706734113	35.0	33.0	35.0	28.0	37.0
86-87	33.05510881383613	35.0	32.5	35.0	28.0	36.5
88-89	32.94643562056601	35.0	32.0	35.0	28.0	36.0
90-91	32.8200064283088	34.0	32.0	35.0	28.0	36.0
92-93	32.81721249545888	34.0	32.0	35.0	28.5	35.5
94-95	32.76000926994139	34.0	32.0	35.0	29.0	35.0
96-97	32.866990521188086	34.0	32.0	35.0	29.0	35.0
98-99	32.995832293517466	34.0	32.0	35.0	29.0	35.0
100	31.92340445917454	34.0	31.0	35.0	26.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	1.0
18	5.0
19	21.0
20	161.0
21	807.0
22	3569.0
23	11798.0
24	30653.0
25	63815.0
26	113617.0
27	176462.0
28	249389.0
29	324830.0
30	405417.0
31	494058.0
32	601523.0
33	743322.0
34	962299.0
35	1281743.0
36	1871464.0
37	2844118.0
38	3002960.0
39	1514411.0
40	55472.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.51980166642772	19.71394900255323	13.461384504994776	24.304864826024282
2	15.898124691057081	21.103048580265778	39.358514514318	23.640312214359138
3	18.89754758931616	25.43053288144105	30.032986275049584	25.63893325419321
4	13.304197231057985	17.17916506574468	37.34468119259898	32.171956510598356
5	13.633795094820226	37.714009488665745	34.91242086790624	13.73977454860779
6	31.48799789803575	36.63666468816661	18.023001215570915	13.852336198226725
7	27.812495678527455	32.10819530154592	22.158043741572282	17.92126527835435
8	25.76772400276683	35.672871239234276	20.4970120491467	18.062392708852194
9	26.369505247284845	15.968699656959792	20.58138892475994	37.08040617099543
10-11	25.310740154388768	26.117323543508636	28.0243741905924	20.5475621115102
12-13	25.732874292397	23.66823401109388	28.311491876716214	22.2873998197929
14-15	22.855695490673888	24.961954094640994	25.940474444133223	26.24187597055189
16-17	22.577097781738996	28.03890694605365	26.56842338310495	22.815571889102408
18-19	22.550379537651207	27.294907769434147	28.198722336956656	21.955990355957994
20-21	23.51747018385257	26.416966968147747	28.05178075230436	22.01378209569532
22-23	23.15287520974009	26.298666362547635	27.910099466315845	22.63835896139643
24-25	22.78164667586223	26.461018092418087	28.072252481315136	22.685082750404547
26-27	22.787536372230164	26.61005386659096	28.00164365432994	22.60076610684893
28-29	22.771071360514348	26.694926476910002	27.96095750950388	22.57304465307177
30-31	23.058039664654967	26.588337252650447	27.777832589172263	22.575790493522323
32-33	22.813800616270356	26.819701289186565	27.724754923195604	22.64174317134748
34-35	22.92233429098414	26.770043814238598	27.775673155379415	22.531948739397844
36-37	22.954680488412713	26.672715129874597	27.746562352070942	22.62604202964175
38-39	22.723924630481136	26.902810927224408	27.83136295951157	22.541901482782887
40-41	23.044436750308922	26.753772688318488	27.59421888738839	22.607571673984204
42-43	22.850449583235548	26.85807499465158	27.431554646188427	22.85992077592444
44-45	22.926063447628266	26.962748887934616	27.293401736416374	22.81778592802074
46-47	23.041956449419025	26.84179036823892	27.087312133873525	23.028941048468525
48-49	22.907584907800214	26.99717845257461	27.051484417178496	23.04375222244668
50-51	22.883875375710154	27.127676312357345	26.956561093481074	23.031887218451427
52-53	23.121227321403392	27.041721019950476	26.76969315074914	23.067358507896994
54-55	23.116565240102645	26.80467913885901	26.928128522260213	23.150627098778127
56-57	23.0518677390638	27.01074365251198	26.880452798274085	23.056935810150133
58-59	22.98486458925735	27.147835977487105	26.854850665777658	23.01244876747789
60-61	23.10707215518948	27.01980540686062	26.917566752607847	22.95555568534206
62-63	23.176560221898583	27.10290733899346	26.761396454603997	22.959135984503956
64-65	23.036856135647994	27.10180261055653	26.83954418771456	23.021797066080918
66-67	23.18091661962634	27.084643555601957	26.650425795308447	23.08401402946326
68-69	23.049213397177446	27.089784649892344	26.768652602983316	23.09234934994689
70-71	23.039059887440622	26.767494220980133	26.883412554112756	23.310033337466493
72-73	23.139777504693683	26.87491504454943	26.746977310943503	23.238330139813385
74-75	22.881323871154315	27.09282569415557	26.785502710588975	23.240347724101138
76-77	23.07544420232419	26.931881227058675	26.76411070263424	23.228563867982896
78-79	22.9648638545796	26.972900516253333	26.750418442910984	23.31181718625608
80-81	23.100100096757302	26.926928909710217	26.68065090477066	23.29232008876182
82-83	23.088146676784362	26.868239372703062	26.863733837730063	23.179880112782513
84-85	23.193950714696104	26.87170799917548	26.72539973861241	23.208941547516
86-87	22.944537507368814	26.913077179075103	26.851465424017228	23.290919889538856
88-89	23.027253006254536	26.818615529377926	26.863234527600454	23.290896936767087
90-91	23.04771546136294	26.814075579389907	26.94626438459899	23.19194457464816
92-93	23.160183217032948	26.785226944750555	26.862336653163243	23.192253185053254
94-95	22.959769946749024	26.847463356245566	26.962142974504168	23.230623722501246
96-97	22.8753066968103	26.965314778316756	27.090374540207584	23.06900398466536
98-99	24.069498869893053	28.964153719297215	27.210961745823344	19.75538566498639
100	31.308866236268013	40.51696946188836	28.174164301843625	0.0
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3397.0
1	3062.0
2	4219.0
3	5992.0
4	7909.5
5	12543.0
6	16775.0
7	20331.0
8	22876.5
9	22706.5
10	20340.5
11	17788.0
12	18784.0
13	24494.5
14	37839.0
15	55797.5
16	70453.5
17	78566.0
18	80051.5
19	76792.5
20	70752.0
21	64362.0
22	61181.5
23	63764.5
24	73449.0
25	90840.0
26	116131.5
27	143147.0
28	167261.5
29	195232.5
30	221458.5
31	244810.0
32	274078.0
33	306631.0
34	337492.0
35	368779.0
36	405008.0
37	435860.5
38	457239.0
39	475563.5
40	497784.5
41	520473.5
42	537956.5
43	558269.5
44	579522.0
45	595991.5
46	609879.5
47	624477.0
48	639160.5
49	649488.5
50	654268.0
51	655944.5
52	646775.5
53	629796.0
54	612357.5
55	586666.5
56	551310.0
57	510783.0
58	466564.0
59	413947.5
60	357822.5
61	301318.5
62	245924.5
63	194800.5
64	150156.0
65	117655.5
66	92069.5
67	69221.0
68	51047.5
69	37436.5
70	25998.5
71	17144.0
72	11559.0
73	7809.0
74	5140.5
75	3421.0
76	2352.0
77	1557.0
78	1032.5
79	716.0
80	479.5
81	307.0
82	238.0
83	185.0
84	130.0
85	91.0
86	63.5
87	47.0
88	33.0
89	21.5
90	16.0
91	12.5
92	9.5
93	6.5
94	3.5
95	2.5
96	2.0
97	1.5
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	6.778780464856226E-6
2	0.0
3	1.559119506916932E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	6.778780464856226E-6
10-11	3.389390232428113E-6
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.001474384751106229
20-21	0.0030675502702850743
22-23	0.00520360908766427
24-25	0.0011596895572091644
26-27	0.001876081142646727
28-29	0.0043393602451989805
30-31	0.008256182365087022
32-33	0.01495631132446021
34-35	2.571251522395172E-4
36-37	9.040866233689691E-4
38-39	0.0019776407518781233
40-41	0.0028304272405099465
42-43	3.0907397493751113E-4
44-45	2.5164927338788802E-5
46-47	0.004026158736682478
48-49	0.003492294249047833
50-51	9.230801082926192E-4
52-53	0.001149107510120651
54-55	0.0010056329977934958
56-57	0.0014905869242153086
58-59	7.255845220663E-4
60-61	4.449803102172286E-4
62-63	0.0017623461704157363
64-65	5.418954001718256E-4
66-67	0.002184545082733554
68-69	0.0017760069090019715
70-71	5.908526990618875E-4
72-73	8.322977407601258E-4
74-75	0.008234897229094842
76-77	0.0031812027741685445
78-79	0.006655196134438744
80-81	1.2351396686788158E-4
82-83	0.00505394117444945
84-85	0.0029763452832651583
86-87	7.493654891829092E-4
88-89	0.0037372701071368697
90-91	8.935306335496593E-4
92-93	5.100183152677196E-5
94-95	6.950548312033213E-4
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	455.0
20-21	1466.0
22-23	3077.0
24-25	6677.0
26-27	10283.0
28-29	21070.0
30-31	46004.0
32-33	59899.0
34-35	84468.0
36-37	122113.0
38-39	141649.0
40-41	143066.0
42-43	158383.0
44-45	183593.0
46-47	182326.0
48-49	169041.0
50-51	154157.0
52-53	148709.0
54-55	159180.0
56-57	166658.0
58-59	163961.0
60-61	160567.0
62-63	154211.0
64-65	161028.0
66-67	169575.0
68-69	174012.0
70-71	171156.0
72-73	161915.0
74-75	164097.0
76-77	162055.0
78-79	172835.0
80-81	177417.0
82-83	170502.0
84-85	173049.0
86-87	182830.0
88-89	201066.0
90-91	211096.0
92-93	228604.0
94-95	262498.0
96-97	559337.0
98-99	2983928.0
100-101	5823903.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.1042016993355
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.98181726917699	73.05781072641922
2	7.215908839979514	11.84912869683392
3	1.9499371506746854	4.802940993600658
4	0.7818230214482755	2.5676382018469246
5	0.3904200951264333	1.6027565118867215
6	0.20691432236156349	1.019312115459308
7	0.13680872980638936	0.7862800082377578
8	0.07946264487903858	0.5219373618169002
9	0.05358858414985534	0.39598631296393827
>10	0.19491945297976415	2.5400826050738434
>50	0.005695211719413157	0.32597883567687197
>100	0.0025535011096115352	0.36425274409077885
>500	5.039392591542902E-5	0.03458951069811929
>1k	1.0078266264416739E-4	0.13130537539515896
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.3557560929712453E-5	0.0
2	0.0	8.812414604313094E-5	0.0	2.0336341394568678E-5	6.778780464856226E-6
3	0.0	1.0168170697284339E-4	0.0	3.389390232428113E-5	6.778780464856226E-6
4	0.0	3.5927536463737996E-4	0.0	4.0672682789137356E-5	6.778780464856226E-6
5	0.0	3.7961170603194864E-4	0.0	6.1009024183706034E-5	6.778780464856226E-6
6	0.0	3.863904864968049E-4	0.0	6.1009024183706034E-5	6.778780464856226E-6
7	0.0	3.863904864968049E-4	0.0	1.0846048743769962E-4	6.778780464856226E-6
8	0.0	4.677358520750796E-4	0.0	1.0846048743769962E-4	6.778780464856226E-6
9	0.0	8.066748753178909E-4	0.0	1.4235438976198075E-4	1.3557560929712453E-5
10-11	3.389390232428113E-6	8.066748753178909E-4	0.0	1.49133170226837E-4	1.3557560929712453E-5
12-13	6.778780464856226E-6	8.066748753178909E-4	0.0	1.7285890185383376E-4	1.3557560929712453E-5
14-15	6.778780464856226E-6	0.001108330606003993	0.0	1.7963768231869E-4	2.3725731626996793E-5
16-17	2.0336341394568678E-5	0.0011490032887931303	0.0	1.830270725511181E-4	4.0672682789137356E-5
18-19	2.0336341394568678E-5	0.0012540743859984018	0.0	1.8980585301597432E-4	4.745146325399358E-5
20-21	2.0336341394568678E-5	0.001376092434365814	0.0	2.2031036510782736E-4	4.745146325399358E-5
22-23	2.0336341394568678E-5	0.001535393775289935	0.0	2.4403609673482414E-4	6.439841441613414E-5
24-25	2.0336341394568678E-5	0.0016641906041222035	0.0	2.813193892915334E-4	7.795597534584659E-5
26-27	2.0336341394568678E-5	0.001735367799003194	0.0	3.9316926696166107E-4	8.473475581070283E-5
28-29	2.0336341394568678E-5	0.001738757189235622	0.0	8.981884115934499E-4	8.812414604313094E-5
30-31	2.0336341394568678E-5	0.0019929614566677302	0.0	0.0023183429189808295	9.490292650798717E-5
32-33	2.0336341394568678E-5	0.002009908407829871	0.0	0.00485021742260463	1.0846048743769962E-4
34-35	2.0336341394568678E-5	0.002338679260375398	0.0	0.008463307410372998	1.1523926790255584E-4
36-37	2.0336341394568678E-5	0.002664060722688497	0.0	0.013693136539009578	1.1523926790255584E-4
38-39	2.0336341394568678E-5	0.0027284591371046314	0.0	0.021492123463826666	1.2879682883226829E-4
40-41	2.0336341394568678E-5	0.003019946697093449	0.0	0.029697837216535128	1.2879682883226829E-4
42-43	2.0336341394568678E-5	0.003084345111509583	0.0	0.0375069923120495	1.355756092971245E-4
44-45	2.0336341394568678E-5	0.003487682549168528	0.0	0.04629907057496803	1.4574377999440884E-4
46-47	2.0336341394568678E-5	0.0038300109626437677	0.0	0.055379247007642934	1.4913317022683696E-4
48-49	2.3725731626996793E-5	0.0038469579138059084	0.0	0.06478480490263096	1.6269073115654942E-4
50-51	2.7115121859424905E-5	0.004250295351464854	0.0	0.07373957389670603	1.6269073115654942E-4
52-53	2.7115121859424905E-5	0.004280799863556707	0.0	0.0825723248424137	1.864164627835462E-4
54-55	3.0504512091853017E-5	0.004318083156113416	0.0	0.09136779249556465	2.10142194410543E-4
56-57	3.389390232428113E-5	0.004379092180297122	0.0	0.10054626124497998	2.1692097487539921E-4
58-59	3.389390232428113E-5	0.004419764863086259	0.0	0.10953831353161175	2.2708914557268355E-4
60-61	4.4062073021565465E-5	0.00447060571657268	0.0	0.11787960289361735	2.3047853580511168E-4
62-63	6.1009024183706034E-5	0.004558729862615812	0.0	0.12582772298866127	2.3047853580511168E-4
64-65	6.1009024183706034E-5	0.004989182422134183	0.0	0.1335962054013865	2.3047853580511168E-4
66-67	6.778780464856226E-5	0.005016297543993607	0.0	0.14154432549643042	2.3047853580511168E-4
68-69	6.778780464856226E-5	0.005090864129107026	0.0	0.1492687458361341	2.3047853580511168E-4
70-71	6.778780464856226E-5	0.00513831559236102	0.0	0.15609158837401188	2.3047853580511168E-4
72-73	7.117719488099037E-5	0.0051552625435231595	0.0	0.16137903713659973	2.4403609673482414E-4
74-75	7.456658511341848E-5	0.005250165470031147	0.0	0.16548019931783775	2.4403609673482414E-4
76-77	7.456658511341848E-5	0.0054433607132795495	0.0	0.16938816625582737	2.4403609673482414E-4
78-79	7.456658511341848E-5	0.005538263639787537	0.0	0.17184208478410534	2.4403609673482414E-4
80-81	7.795597534584659E-5	0.005555210590949678	0.0	0.1727775564882555	2.4403609673482414E-4
82-83	8.134536557827471E-5	0.0055721575421118184	0.0	0.17341137246171956	2.4403609673482414E-4
84-85	8.134536557827471E-5	0.0055823257128091025	0.0	0.1736587979486868	2.4403609673482414E-4
86-87	8.134536557827471E-5	0.005673839249084661	0.0	0.17377403721658935	2.4403609673482414E-4
88	8.812414604313094E-5	0.005917875345819485	0.0	0.17378081599705422	2.508148771996804E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	18060	0.0	50.53494	1
GTATCAA	32055	0.0	34.852222	1
TCAACGC	40000	0.0	27.442636	4
ATCAACG	40970	0.0	26.822718	3
TATCAAC	41430	0.0	26.74039	2
CAACGCA	41780	0.0	26.254042	5
AACGCAG	42375	0.0	25.981167	6
GTACATG	53915	0.0	22.798681	1
TACATGG	53965	0.0	22.371485	2
ACGCAGA	50450	0.0	21.806547	7
ACATGGG	55760	0.0	21.272871	3
CGCAGAG	51840	0.0	21.2375	8
GTGGTAT	8715	0.0	20.208885	1
CATGGGG	41750	0.0	19.50785	4
TGGTATC	9040	0.0	18.494766	2
GCAGAGT	60235	0.0	18.183292	9
GAGTACT	30900	0.0	17.775566	12-13
CAGAGTA	57310	0.0	17.312788	10-11
AGAGTAC	53580	0.0	16.704096	10-11
CGTCGAA	1140	0.0	16.189758	94
>>END_MODULE