FastQCFastQC Report
Fri 27 May 2016
SRR2138724_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138724_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10641795
Sequences flagged as poor quality0
Sequence length19-100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA120000.049.9329641
GTATCAA221100.035.6306421
TCAACGC273450.028.273154
ATCAACG280050.027.5631923
TATCAAC283950.027.342422
CAACGCA283350.027.2565575
AACGCAG288850.026.751676
GTACATG363900.023.2045521
TACATGG367000.022.6422732
ACGCAGA347050.022.2536967
CGCAGAG353000.021.855528
ACATGGG376700.021.5942613
CATGGGG288700.019.2311384
GTGGTAT60850.018.475851
GCAGAGT415350.018.3687469
GAGTACT209500.018.36453812-13
TGGTATC60500.017.6401332
AGAGTAC359050.017.1478310-11
CAGAGTA393550.017.06258810-11
CGTCGAA7902.5211193E-916.79964494
Produced by FastQC (version 0.11.5)