FastQCFastQC Report
Fri 27 May 2016
SRR2138723_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138723_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24953700
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA298800.044.270361
GTATCAA486950.032.861261
TCAACGC586450.026.5850894
ATCAACG601550.025.9178093
CAACGCA603350.025.8744895
AACGCAG618300.025.275456
TATCAAC620800.025.2730052
GTACATG730150.021.2403891
ACGCAGA734600.021.2067787
TACATGG730550.020.9193822
GTGGTAT143150.020.8983821
CGCAGAG751550.020.76138
ACATGGG741750.020.0993673
TGGTATC150250.018.1877862
CATGGGG545900.017.8480284
GCAGAGT890100.017.4419469
GAGTACT490450.017.2604512-13
AGAGTAC782950.016.75068710-11
CAGAGTA852050.016.59554110-11
GTACTTT545200.015.3817314-15
Produced by FastQC (version 0.11.5)