FastQCFastQC Report
Fri 27 May 2016
SRR2138722_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138722_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12273914
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA195550.034.395191
GTATCAA299650.026.5594251
TCAACGC321850.023.8651624
CAACGCA326850.023.5250595
ATCAACG329950.023.3040333
AACGCAG334300.023.1350866
GTACATG363700.021.6015931
TACATGG365300.021.104772
TATCAAC378350.020.7867342
ACATGGG374300.020.1829623
ACGCAGA390750.019.6779677
CGCAGAG406250.018.9573158
CATGGGG282750.017.6964514
GAGTACT234000.017.31098712-13
CAGAGTA412200.016.75787710-11
GCAGAGT462200.016.424099
AGAGTAC380750.016.24479910-11
GTACTTT259600.015.62117514-15
GAGTACA263000.015.2854711
ATGGGGG171700.014.618515
Produced by FastQC (version 0.11.5)