##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138722_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	12273914
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.751042414017242	31.0	31.0	34.0	30.0	34.0
2	32.02396277177761	33.0	31.0	34.0	30.0	34.0
3	31.930904273893397	31.0	31.0	34.0	30.0	34.0
4	35.78782196127494	37.0	35.0	37.0	35.0	37.0
5	35.56469118163937	37.0	35.0	37.0	33.0	37.0
6	35.64955587924113	37.0	35.0	37.0	33.0	37.0
7	35.56548620106023	37.0	35.0	37.0	33.0	37.0
8	35.55262697783282	37.0	35.0	37.0	33.0	37.0
9	37.27358363436472	39.0	37.0	39.0	34.0	39.0
10-11	37.14787234943964	39.0	37.0	39.0	33.0	39.0
12-13	37.10768288746361	39.0	37.0	39.0	33.0	39.0
14-15	38.28940743759489	40.0	38.0	41.0	33.0	41.0
16-17	38.24344728177173	40.0	38.0	41.0	33.0	41.0
18-19	38.24275699666789	40.0	38.0	41.0	33.0	41.0
20-21	38.2424368920054	40.0	38.0	41.0	33.0	41.0
22-23	38.17442362649851	40.0	37.5	41.0	33.0	41.0
24-25	38.092385385104826	40.0	37.0	41.0	33.0	41.0
26-27	37.92441976006276	40.0	37.0	41.0	33.0	41.0
28-29	37.777276113096676	40.0	37.0	41.0	32.0	41.0
30-31	37.62084773269946	40.0	37.0	41.0	32.0	41.0
32-33	37.46117235505195	39.0	36.0	41.0	32.0	41.0
34-35	37.33222634026394	39.0	36.0	41.0	31.0	41.0
36-37	37.41918104826773	39.0	36.0	41.0	31.0	41.0
38-39	37.38824861845596	39.0	36.0	41.0	31.0	41.0
40-41	37.35396259516028	39.0	36.0	41.0	31.0	41.0
42-43	37.440776120287694	39.0	36.0	41.0	31.0	41.0
44-45	37.4788561990718	40.0	36.0	41.0	31.5	41.0
46-47	37.361200899557375	39.0	36.0	41.0	31.0	41.0
48-49	37.29893391602382	39.0	36.0	41.0	31.0	41.0
50-51	37.204074610375116	39.0	36.0	41.0	31.0	41.0
52-53	37.04951401263193	39.0	35.0	41.0	31.0	41.0
54-55	36.877703419088014	39.0	35.0	40.5	31.0	41.0
56-57	36.73516772650705	39.0	35.0	40.0	30.5	41.0
58-59	36.473244607678296	38.0	35.0	40.0	30.0	41.0
60-61	36.31016364669519	38.0	35.0	40.0	30.0	41.0
62-63	36.0506555885552	38.0	34.0	40.0	29.5	41.0
64-65	35.74089514119838	37.0	34.0	40.0	29.0	41.0
66-67	35.41054037466087	36.5	34.0	39.5	29.0	41.0
68-69	35.177777899212316	36.0	34.0	39.0	29.0	41.0
70-71	34.852514527237446	36.0	33.5	39.0	28.5	40.0
72-73	34.555564604276185	35.0	33.0	38.5	28.0	40.0
74-75	34.27552388278601	35.0	33.0	37.5	28.0	39.5
76-77	32.91469196177157	34.0	31.5	36.0	26.5	39.0
78-79	33.665951057396526	35.0	32.5	36.0	28.0	39.0
80-81	33.69871667378224	35.0	33.0	36.0	29.0	38.5
82-83	33.471801410242946	35.0	33.0	36.0	29.0	37.0
84-85	33.269666335904304	35.0	33.0	35.0	28.0	37.0
86-87	33.099579854280734	35.0	32.5	35.0	28.0	36.5
88-89	32.98605236999498	35.0	32.0	35.0	28.0	36.0
90-91	32.85108786615358	34.5	32.0	35.0	28.0	36.0
92-93	32.84367521241377	34.0	32.0	35.0	29.0	36.0
94-95	32.783822145181176	34.0	32.0	35.0	29.0	35.0
96-97	32.88630544088494	34.0	32.0	35.0	29.0	35.0
98-99	33.011910493929776	34.0	32.0	35.0	29.0	35.0
100	31.96447544704735	34.0	31.0	35.0	26.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	1.0
18	2.0
19	20.0
20	99.0
21	611.0
22	2886.0
23	9758.0
24	24977.0
25	52354.0
26	93317.0
27	144622.0
28	205354.0
29	269249.0
30	336271.0
31	410890.0
32	499247.0
33	618748.0
34	795017.0
35	1057039.0
36	1539267.0
37	2360857.0
38	2545408.0
39	1263399.0
40	44520.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.81842890540051	20.100238603594583	13.719128225926955	24.362204265077953
2	15.641848231949481	21.090542104173128	39.62424700059003	23.64336266328736
3	18.658447490891035	25.486970358807742	30.270690674577004	25.58389147572422
4	13.251624542912719	17.381945156206896	37.620729622188975	31.745700678691406
5	13.540269224633642	37.55795421085727	35.2606348716473	13.641141692861789
6	31.259009962103367	36.940172466582375	18.025643653687	13.775173917627253
7	27.376955712741672	32.11167195729088	22.570974507398372	17.940397822569068
8	25.236578975541136	36.03908256160178	20.684950212295767	18.039388250561313
9	25.836762421506293	16.26830691497431	20.969504919131747	36.92542574438765
10-11	24.986243182085193	26.243820838242797	28.29669085183422	20.473245127837785
12-13	25.432632980807917	23.754354153043604	28.577607762283492	22.235405103864995
14-15	22.49003862989426	25.246995375721227	26.093591661144117	26.1693743332404
16-17	22.27392582349852	28.187536591832075	26.884806264733484	22.653731319935922
18-19	22.261499918891726	27.64646194791684	28.32819511807222	21.763843015119217
20-21	23.081726209294974	26.74947687664393	28.182321526933517	21.98647538712758
22-23	22.824046045233576	26.604119198843318	28.06854021918373	22.503294536739375
24-25	22.571542887906755	26.81140026435802	28.123742020876357	22.493314826858864
26-27	22.5450885622309	26.888760748625508	28.11249120168471	22.453659487458882
28-29	22.50141038041619	26.995550536515978	28.15126249352972	22.351776589538115
30-31	22.72635950377643	26.92050808350724	27.951731429005513	22.401400983710822
32-33	22.50904680420073	27.06468421941078	27.92053182855931	22.505737147829187
34-35	22.65871644068163	27.092335931363415	27.867466493441782	22.38148113451317
36-37	22.703599844257784	26.957990617186116	27.82570908239116	22.51270045616494
38-39	22.57056034862487	27.123608834136892	27.930662669709278	22.375168147528964
40-41	22.807341564667123	26.952223941620172	27.814813324029895	22.425621169682803
42-43	22.58316948991411	27.14100673465179	27.57348357138681	22.702340204047296
44-45	22.69624261295215	27.22384269135431	27.39001912935303	22.689895566340514
46-47	22.755104277560896	27.16096964223482	27.239664096960215	22.844261983244067
48-49	22.751706851281366	27.216237573722086	27.222250751360445	22.809804823636103
50-51	22.734126158583436	27.328793647045234	27.13477752583081	22.802302668540516
52-53	22.877990949562506	27.264908677167725	26.980530278252484	22.87657009501728
54-55	22.85101420920034	27.192069681511256	27.02683114745455	22.930084961833856
56-57	22.840603983309872	27.331202329311683	27.007742734128577	22.820450953249864
58-59	22.810383589924278	27.28317595450045	27.056040067853225	22.850400387722047
60-61	22.861686798184863	27.316756285085138	27.04887625784313	22.77268065888687
62-63	22.91031617500027	27.368092382543868	26.98413147172733	22.737459970728537
64-65	22.80966398038423	27.302693782333602	27.026085377152132	22.861556860130037
66-67	22.878941342593798	27.30356096169843	26.873993400744993	22.94350429496278
68-69	22.816035833828874	27.322467023289487	26.97483529528059	22.886661847601044
70-71	22.771622596417608	27.114436011345393	27.047797438154152	23.066143954082843
72-73	22.903499402348455	27.106961422631176	26.98516460159384	23.00437457342653
74-75	22.719451784857792	27.31135152138281	26.957585336908725	23.011611356850675
76-77	22.838973155054784	27.171823092176005	26.98639345849737	23.00281029427184
78-79	22.774655825502286	27.106145604238268	27.002458370153576	23.11674020010587
80-81	22.75898617714432	27.215200917468447	26.928630646161956	23.097182259225278
82-83	22.85689857935979	27.1349907828138	27.038762221118468	22.969348416707945
84-85	22.941441898203628	27.12456428205113	26.95355706954669	22.980436750198553
86-87	22.718617967851078	27.126178291010778	27.12619540655347	23.02900833458467
88-89	22.776064496911914	27.035655320056563	27.12616503227959	23.062115150751936
90-91	22.876215634747062	27.064433797360156	27.066987975569017	22.992362592323765
92-93	22.929562953371484	27.043208273479063	27.087338499414926	22.939890273734527
94-95	22.70387599630518	27.049634019457553	27.20421558570503	23.042274398532246
96-97	22.70044084058197	27.172007730684527	27.303912887534626	22.823638541198882
98-99	23.867711568011757	29.184673499409413	27.439799575139755	19.507815357439075
100	30.858477535986268	40.77366694076459	28.36785552324914	0.0
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2194.0
1	1965.5
2	2819.0
3	4106.0
4	5535.0
5	8989.5
6	12071.5
7	14419.5
8	15997.5
9	15957.0
10	14525.0
11	12822.0
12	13562.0
13	17863.0
14	28037.0
15	41823.0
16	53276.5
17	60281.0
18	62621.0
19	61024.0
20	57444.0
21	53578.5
22	51760.0
23	54542.0
24	63101.5
25	77374.5
26	97392.5
27	119821.0
28	141423.5
29	165502.0
30	189741.0
31	212542.5
32	238605.5
33	266945.5
34	293341.5
35	319503.0
36	348654.0
37	374562.5
38	396654.0
39	414850.5
40	432092.5
41	450787.0
42	466374.5
43	485553.0
44	504757.0
45	521195.0
46	534443.5
47	542053.5
48	546663.0
49	547085.0
50	543628.0
51	537719.0
52	525021.5
53	507142.5
54	488004.0
55	463188.5
56	432390.0
57	396578.5
58	356700.5
59	311403.5
60	264947.5
61	220366.5
62	178638.0
63	140639.5
64	106422.0
65	79706.5
66	59511.0
67	45341.5
68	34503.5
69	25068.5
70	17876.0
71	12421.5
72	8502.0
73	5707.5
74	3798.0
75	2538.0
76	1739.0
77	1196.0
78	847.0
79	596.0
80	396.0
81	260.0
82	187.5
83	139.5
84	98.5
85	67.0
86	44.5
87	30.5
88	20.0
89	14.0
90	10.5
91	7.5
92	6.0
93	4.0
94	2.5
95	1.5
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	1.2221040492869673E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0016050299847302173
20-21	0.003332559538539096
22-23	0.004979403727405599
24-25	0.0012228080106641902
26-27	0.0017331259291339081
28-29	0.004607860274983551
30-31	0.007976038361841914
32-33	0.014926085532374926
34-35	2.1824640414342032E-4
36-37	9.86015156461548E-4
38-39	0.0020467808043539456
40-41	0.0030261237508441433
42-43	3.3192704073425666E-4
44-45	8.606164156470908E-6
46-47	0.0040526568237655115
48-49	0.0038336397965868076
50-51	7.98762783383967E-4
52-53	0.0011592234447844732
54-55	0.0011311331819925787
56-57	0.0015697417322395495
58-59	7.013059438764489E-4
60-61	5.349523927876108E-4
62-63	0.0016819091077151134
64-65	4.8511384506181763E-4
66-67	0.0020155544763326466
68-69	0.0016798819038036812
70-71	5.915977644885706E-4
72-73	7.178945997404298E-4
74-75	0.008531338189004336
76-77	0.0034175520833882706
78-79	0.006797121445828013
80-81	1.1959213212060389E-4
82-83	0.004777232933901432
84-85	0.0028449888467458936
86-87	6.96027224933749E-4
88-89	0.00403101066963219
90-91	0.001031142518948429
92-93	4.8428208026757554E-5
94-95	6.513225818377519E-4
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	517.0
20-21	2030.0
22-23	3292.0
24-25	5285.0
26-27	8856.0
28-29	15552.0
30-31	34415.0
32-33	46898.0
34-35	66159.0
36-37	94247.0
38-39	106886.0
40-41	110969.0
42-43	124041.0
44-45	144178.0
46-47	142641.0
48-49	132236.0
50-51	121616.0
52-53	119585.0
54-55	130950.0
56-57	135884.0
58-59	133335.0
60-61	128548.0
62-63	126031.0
64-65	134058.0
66-67	139954.0
68-69	141784.0
70-71	140036.0
72-73	132779.0
74-75	137163.0
76-77	135642.0
78-79	144035.0
80-81	145573.0
82-83	142446.0
84-85	145026.0
86-87	152142.0
88-89	167581.0
90-91	175884.0
92-93	192499.0
94-95	222330.0
96-97	470685.0
98-99	2496115.0
100-101	4924031.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.66295171471303
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.25045001562816	74.66956090173908
2	7.235250208306537	12.106447776428345
3	1.8954579216786818	4.757388137360215
4	0.7300683337070694	2.4431868700550217
5	0.3510025405689526	1.468295430168094
6	0.1794357061985584	0.9007272494151253
7	0.10766584461837124	0.6305349651741412
8	0.06275099317604618	0.41999466497102705
9	0.04445013186078049	0.33469463120229953
>10	0.13782576163862675	1.7924084214197191
>50	0.004200393752929631	0.2351066693813639
>100	0.0013534564290335288	0.17941585682970818
>500	5.322020621850189E-5	0.024919454414267926
>1k	3.5472229979129445E-5	0.03731897144164639
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	4.073680164289892E-5	0.0	0.0	0.0
3	0.0	4.073680164289892E-5	0.0	1.6294720657159565E-5	0.0
4	0.0	1.466524859144361E-4	0.0	2.444208098573935E-5	8.147360328579782E-6
5	8.147360328579782E-6	1.466524859144361E-4	0.0	2.444208098573935E-5	8.147360328579782E-6
6	8.147360328579783E-5	1.466524859144361E-4	0.0	2.444208098573935E-5	8.147360328579782E-6
7	8.962096361437761E-5	1.466524859144361E-4	0.0	3.258944131431913E-5	8.147360328579782E-6
8	8.962096361437761E-5	1.7109456690017546E-4	0.0	3.258944131431913E-5	1.6294720657159565E-5
9	8.962096361437761E-5	2.770102511717126E-4	0.0	5.703152230005848E-5	3.258944131431913E-5
10-11	9.36946437786675E-5	2.770102511717126E-4	0.0	9.36946437786675E-5	3.258944131431913E-5
12-13	9.77683239429574E-5	2.770102511717126E-4	0.0	1.385051255858563E-4	3.258944131431913E-5
14-15	1.0591568427153718E-4	3.421891338003509E-4	0.0	1.7516824706446534E-4	5.295784213576859E-5
16-17	1.466524859144361E-4	3.421891338003509E-4	0.0	1.996103280502047E-4	9.77683239429574E-5
18-19	1.466524859144361E-4	3.6663121478609025E-4	0.0	2.4849449002168337E-4	1.0184200410724729E-4
20-21	1.5072616607872597E-4	3.951469759361195E-4	0.0	3.910732957718296E-4	1.0591568427153718E-4
22-23	1.6294720657159567E-4	4.195890569218588E-4	0.0	6.314204254649332E-4	1.0998936443582706E-4
24-25	1.6702088673588555E-4	4.521784982361779E-4	0.0	7.984413122008188E-4	1.1406304460011696E-4
26-27	1.7109456690017546E-4	4.725468990576274E-4	0.0	9.04356996472356E-4	1.1406304460011696E-4
28-29	1.7109456690017546E-4	4.766205792219173E-4	0.0	0.0014176406971728823	1.1406304460011696E-4
30-31	1.7109456690017546E-4	5.703152230005849E-4	0.0	0.00262345002580269	1.1406304460011696E-4
32-33	1.7516824706446534E-4	5.743889031648747E-4	0.0	0.005381331497026947	1.1813672476440685E-4
34-35	1.9146296772162488E-4	6.721572271078322E-4	0.0	0.008676938749937468	1.2221040492869676E-4
36-37	2.0775768837878446E-4	7.699255510507895E-4	0.0	0.013707933752835485	1.2221040492869676E-4
38-39	2.1183136854307437E-4	7.90293951872239E-4	0.0	0.021443852384821986	1.2221040492869676E-4
40-41	2.1183136854307437E-4	8.391781138437177E-4	0.0	0.029420118146501598	1.2221040492869676E-4
42-43	2.1590504870736427E-4	8.595465146651671E-4	0.0	0.03724973142226677	1.2221040492869676E-4
44-45	2.2812608920023394E-4	9.858305997581538E-4	0.0	0.04576372296563264	1.2221040492869676E-4
46-47	2.973786519931621E-4	0.001091746284029691	0.0	0.05508430318152791	1.3035776525727652E-4
48-49	3.01452332157452E-4	0.0010998936443582707	0.0	0.06417675730822295	1.466524859144361E-4
50-51	3.01452332157452E-4	0.0011772935674797787	0.0	0.07355436904641828	1.5072616607872597E-4
52-53	3.01452332157452E-4	0.0011895146079726484	0.0	0.08282199142017779	1.6702088673588555E-4
54-55	3.055260123217419E-4	0.0011935882881369381	0.0	0.09114859367598632	1.8331560739304512E-4
56-57	3.421891338003509E-4	0.0012139566889583877	0.0	0.09934483816653758	1.87389287557335E-4
58-59	3.7477857511467E-4	0.0012343250897798373	0.0	0.10761440890004606	1.9146296772162488E-4
60-61	4.0329433626469925E-4	0.0012587671707655766	0.0	0.11572103242698295	1.955366478859148E-4
62-63	4.4810481807188807E-4	0.001275061891422736	0.0	0.12282960431366882	1.955366478859148E-4
64-65	4.4810481807188807E-4	0.0014013459765157227	0.0	0.1301703759697192	1.955366478859148E-4
66-67	4.6847321889333755E-4	0.001421714377337172	0.0	0.13799184188515579	1.955366478859148E-4
68-69	4.88841619714787E-4	0.0014543038186514913	0.0	0.14545075026597057	1.955366478859148E-4
70-71	4.929152998790769E-4	0.0014828195798015204	0.0	0.1520460384519559	2.1183136854307437E-4
72-73	5.540205023434252E-4	0.0014909669401301003	0.0	0.1572847911432327	2.362734495288137E-4
74-75	5.62167862672005E-4	0.0015235563814444194	0.0	0.16179028140493734	2.362734495288137E-4
76-77	5.703152230005849E-4	0.001580587903744478	0.0	0.16572545644364137	2.403471296931036E-4
78-79	5.906836238220342E-4	0.0016050299847302173	0.0	0.16855666415782283	2.444208098573935E-4
80-81	6.029046643149039E-4	0.0016091036648945073	0.0	0.16948138955511666	2.444208098573935E-4
82-83	6.151257048077736E-4	0.001617251025223087	0.0	0.17009651525992442	2.6071553051455304E-4
84-85	6.191993849720635E-4	0.001621324705387377	0.0	0.1703898202317533	2.6071553051455304E-4
86-87	6.436414659578029E-4	0.0016457667863731163	0.0	0.17048758855569623	2.6071553051455304E-4
88	6.436414659578029E-4	0.0017516824706446533	0.0	0.17049980959618913	2.6071553051455304E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	19555	0.0	34.39519	1
GTATCAA	29965	0.0	26.559425	1
TCAACGC	32185	0.0	23.865162	4
CAACGCA	32685	0.0	23.525059	5
ATCAACG	32995	0.0	23.304033	3
AACGCAG	33430	0.0	23.135086	6
GTACATG	36370	0.0	21.601593	1
TACATGG	36530	0.0	21.10477	2
TATCAAC	37835	0.0	20.786734	2
ACATGGG	37430	0.0	20.182962	3
ACGCAGA	39075	0.0	19.677967	7
CGCAGAG	40625	0.0	18.957315	8
CATGGGG	28275	0.0	17.696451	4
GAGTACT	23400	0.0	17.310987	12-13
CAGAGTA	41220	0.0	16.757877	10-11
GCAGAGT	46220	0.0	16.42409	9
AGAGTAC	38075	0.0	16.244799	10-11
GTACTTT	25960	0.0	15.621175	14-15
GAGTACA	26300	0.0	15.285471	1
ATGGGGG	17170	0.0	14.61851	5
>>END_MODULE