FastQCFastQC Report
Fri 27 May 2016
SRR2138721_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138721_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19971200
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA277950.052.2556151
GTATCAA502200.035.6406441
TCAACGC642200.027.3714624
ATCAACG643450.027.337223
TATCAAC659250.026.8544982
CAACGCA657650.026.7346045
AACGCAG673850.026.1521386
GTACATG870850.023.2528781
TACATGG879300.022.6415182
ACGCAGA801400.021.9644577
ACATGGG895650.021.8201753
TGGTATC134600.021.6600822
GTGGTAT140800.021.6003041
CGCAGAG824200.021.3519218
GCAGAGT932450.018.6945939
CATGGGG571500.018.3806534
GAGTACT463500.018.1652112-13
CAGAGTA902250.017.43465810-11
AGAGTAC850400.016.97211510-11
GTACTTT518100.016.22467814-15
Produced by FastQC (version 0.11.5)