##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138721_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	19971200
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.754347460342895	31.0	31.0	34.0	30.0	34.0
2	32.0211362862522	33.0	31.0	34.0	30.0	34.0
3	31.921580525957378	31.0	31.0	34.0	30.0	34.0
4	35.78327186148053	37.0	35.0	37.0	35.0	37.0
5	35.56951395008813	37.0	35.0	37.0	33.0	37.0
6	35.65718689913476	37.0	35.0	37.0	33.0	37.0
7	35.57382931421247	37.0	35.0	37.0	33.0	37.0
8	35.556550983416116	37.0	35.0	37.0	33.0	37.0
9	37.2749476746515	39.0	37.0	39.0	34.0	39.0
10-11	37.14843299351065	39.0	37.0	39.0	33.0	39.0
12-13	37.10846506469316	39.0	37.0	39.0	33.0	39.0
14-15	38.27740689092293	40.0	38.0	41.0	33.0	41.0
16-17	38.232136776958825	40.0	38.0	41.0	33.0	41.0
18-19	38.244057092212785	40.0	38.0	41.0	33.0	41.0
20-21	38.24978729399264	40.0	38.0	41.0	33.0	41.0
22-23	38.181143915314905	40.0	37.5	41.0	33.0	41.0
24-25	38.099768247936105	40.0	37.0	41.0	33.0	41.0
26-27	37.933241851547535	40.0	37.0	41.0	33.0	41.0
28-29	37.78410314371923	40.0	37.0	41.0	32.5	41.0
30-31	37.62139021756044	39.5	37.0	41.0	32.0	41.0
32-33	37.449020881328906	39.0	36.0	41.0	32.0	41.0
34-35	37.32264473947254	39.0	36.0	41.0	31.0	41.0
36-37	37.40997441672238	39.0	36.0	41.0	31.0	41.0
38-39	37.38066787957412	39.0	36.0	41.0	31.0	41.0
40-41	37.344445215142734	39.0	36.0	41.0	31.0	41.0
42-43	37.428298031829144	39.0	36.0	41.0	31.5	41.0
44-45	37.46845178060797	40.0	36.0	41.0	32.0	41.0
46-47	37.349550851417355	39.0	36.0	41.0	31.0	41.0
48-49	37.28925716737764	39.0	36.0	41.0	31.0	41.0
50-51	37.19557186805405	39.0	36.0	41.0	31.0	41.0
52-53	37.04558780110257	39.0	35.0	41.0	31.0	41.0
54-55	36.87640648338456	39.0	35.0	40.5	31.0	41.0
56-57	36.73875973127613	39.0	35.0	40.0	31.0	41.0
58-59	36.481849825248474	38.0	35.0	40.0	30.0	41.0
60-61	36.324845706805924	38.0	35.0	40.0	30.0	41.0
62-63	36.063927969144885	38.0	34.0	40.0	29.5	41.0
64-65	35.75880330852985	37.0	34.0	40.0	29.0	41.0
66-67	35.42813058388873	37.0	34.0	39.5	29.0	41.0
68-69	35.19675481979983	36.0	34.0	39.0	29.0	41.0
70-71	34.87634769573496	36.0	33.5	39.0	29.0	40.0
72-73	34.57854463609495	35.0	33.0	38.5	28.0	40.0
74-75	34.295348968151146	35.0	33.0	37.5	28.0	39.5
76-77	32.94105827538175	34.0	31.5	36.0	26.5	39.0
78-79	33.69103969384949	35.0	32.5	36.0	28.0	39.0
80-81	33.72208714587704	35.0	33.0	36.0	29.0	38.5
82-83	33.48801742730889	35.0	33.0	36.0	29.0	37.0
84-85	33.28400770252766	35.0	33.0	35.0	29.0	37.0
86-87	33.11450803406646	35.0	32.5	35.0	28.0	36.5
88-89	32.99937762096631	35.0	32.0	35.0	28.0	36.0
90-91	32.86437032197985	34.5	32.0	35.0	28.0	36.0
92-93	32.85669256201271	34.0	32.0	35.0	29.0	36.0
94-95	32.79525681717757	34.0	32.0	35.0	29.0	35.0
96-97	32.8959192106619	34.0	32.0	35.0	29.0	35.0
98-99	33.0212087659663	34.0	32.0	35.0	29.0	35.0
100	31.984900272650254	34.0	31.0	35.0	26.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	1.0
15	0.0
16	0.0
17	0.0
18	7.0
19	36.0
20	200.0
21	1070.0
22	4717.0
23	15599.0
24	39944.0
25	83439.0
26	148637.0
27	232363.0
28	327957.0
29	431899.0
30	543070.0
31	664778.0
32	813337.0
33	1006499.0
34	1297538.0
35	1726012.0
36	2504429.0
37	3819736.0
38	4144256.0
39	2089930.0
40	75745.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.53551614324628	19.968194199647492	13.590395169043424	23.90589448806281
2	15.753705335683385	21.3337055359718	39.616647973081236	23.29594115526358
3	18.78392360024301	26.028424083264458	30.015472809470335	25.172179507022204
4	13.045595657747155	17.486790979009776	37.60422007691075	31.863393286332318
5	13.34755047268066	38.04526518186188	34.983210823585964	13.623973521871493
6	30.90752683864765	36.882610959782085	18.312505007210383	13.897357194359877
7	27.55179458420125	32.323841331517386	22.439232494792503	17.685131589488865
8	25.64172408267906	35.78961704854991	20.6689082278481	17.899750640922928
9	26.444019387918605	15.907682062169522	20.808083640442238	36.84021490946964
10-11	25.034935306841856	26.45714578993751	28.045475484697963	20.46244341852267
12-13	25.620430920525557	24.113170465470276	28.36817517224804	21.89822344175613
14-15	22.720096939593017	25.29742078593174	26.16949907867329	25.81298319580195
16-17	22.110584241307482	28.37609908267906	26.948898914436786	22.56441776157667
18-19	22.126857832804443	27.58152637563581	28.692886821957575	21.59872896960217
20-21	23.136857946162838	26.61804983228028	28.582037540412625	21.663054681144253
22-23	22.844879795678985	26.58783520086115	28.386474548232293	22.180810455227576
24-25	22.49184301519949	26.747310952666293	28.537915264339208	22.22293076779501
26-27	22.33274651107883	26.899305180198635	28.553132572865014	22.214815735857517
28-29	22.285520145835207	26.950554405712708	28.541982996057985	22.221942452394096
30-31	22.59845321569539	26.869000047761816	28.36602513497486	22.166521601567936
32-33	22.32893334576113	27.03554519368181	28.322341304010184	22.313180156546878
34-35	22.48674185012947	27.07029318533855	28.34681448328456	22.096150481247417
36-37	22.51534612400466	26.989439395444602	28.228578920000295	22.26663556055044
38-39	22.315602836697238	27.150727144250773	28.312918674594645	22.220751344457348
40-41	22.56668123975986	26.9856021055811	28.221708056793453	22.226008597865583
42-43	22.38476760613217	27.063345119243888	27.971614215433167	22.580273059190784
44-45	22.435120840522234	27.224781395279745	27.82513054744582	22.514967216752197
46-47	22.56486520006071	27.187210885407442	27.568749372761502	22.679174541770347
48-49	22.57004483516953	27.322095354296817	27.43369410871617	22.67416570181748
50-51	22.591203175961038	27.39558315119582	27.32360188221858	22.689611790624568
52-53	22.717046750488723	27.310819328012514	27.221072973236062	22.751060948262698
54-55	22.692358179007073	27.262616371027775	27.274057634269706	22.770967815695446
56-57	22.66701148581611	27.392720200068275	27.205244795300214	22.735023518815396
58-59	22.613144877814882	27.41360976194119	27.265901100871115	22.707344259372817
60-61	22.746141396041462	27.46633668565642	27.176294877696094	22.611227040606032
62-63	22.770426300318046	27.47448841564016	27.156802734095344	22.598282549946454
64-65	22.65637832723102	27.37040889502475	27.22612649183615	22.74708628590808
66-67	22.755658089716	27.383981501151144	27.049189302863446	22.81117110626941
68-69	22.64493316888175	27.387277450422516	27.155035528515732	22.812753852180006
70-71	22.652313873974826	27.178215611407907	27.252050661362976	22.91741985325429
72-73	22.739186858542322	27.270881095046708	27.14180738118636	22.84812466522461
74-75	22.53055849218208	27.395020996673196	27.170308474941496	22.904112036203227
76-77	22.644213453075064	27.22829865593844	27.196559884389558	22.930928006596943
78-79	22.586213989468437	27.194947608457632	27.18037591118041	23.038462490893522
80-81	22.67429681838027	27.310914309425954	27.06561450249632	22.949174369697456
82-83	22.713300498368824	27.181537199580784	27.243459865349486	22.861702436700902
84-85	22.71608041859968	27.20701940288011	27.131727970854335	22.94517220766587
86-87	22.57566251139836	27.313593322472745	27.24327798753505	22.867466178593844
88-89	22.69553089802655	27.108011822379556	27.246853305000982	22.949603974592907
90-91	22.719513583056415	27.106110301655743	27.2905920474539	22.88378406783394
92-93	22.740996128433395	27.122387969396378	27.282177745936252	22.85443815623397
94-95	22.580683562048602	27.100083890072117	27.358970082686124	22.960262465193157
96-97	22.564640215470842	27.26024825530475	27.41957685806675	22.755534671157662
98-99	23.669609998239576	29.283486098473333	27.607340988333284	19.439562914953807
100	30.571264254622527	40.79397801422402	28.63475773115346	0.0
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	5222.0
1	4668.5
2	6390.5
3	9234.5
4	12385.0
5	19636.5
6	25998.5
7	31200.0
8	35472.0
9	35682.5
10	32058.5
11	27837.0
12	29087.5
13	38151.0
14	59984.5
15	89397.0
16	113227.5
17	126107.5
18	127503.0
19	120468.5
20	108814.5
21	95963.5
22	88073.5
23	90007.5
24	102509.5
25	124543.5
26	155484.0
27	190005.0
28	224790.0
29	266958.0
30	311021.0
31	351919.0
32	399723.5
33	450066.5
34	491195.5
35	536353.0
36	588042.5
37	628516.0
38	661935.0
39	689084.5
40	712032.5
41	735111.0
42	754037.0
43	778421.0
44	805969.0
45	825140.0
46	838027.5
47	853950.0
48	869524.0
49	876608.5
50	875659.0
51	870066.5
52	854730.0
53	826468.5
54	791424.5
55	748857.0
56	698344.5
57	639267.0
58	572119.0
59	498479.5
60	425516.5
61	355177.5
62	288013.5
63	228792.5
64	177228.5
65	136185.0
66	103403.0
67	76677.5
68	56555.5
69	40736.5
70	28047.5
71	18613.5
72	12255.0
73	8051.0
74	5368.0
75	3702.5
76	2594.5
77	1765.0
78	1202.5
79	828.0
80	557.0
81	380.0
82	266.5
83	171.0
84	113.5
85	80.0
86	61.5
87	46.0
88	31.5
89	24.5
90	19.5
91	16.0
92	11.5
93	6.5
94	3.0
95	2.0
96	2.0
97	2.0
98	1.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	1.8526678416920365E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.001584782086204134
20-21	0.003001969106458012
22-23	0.0053160383550038895
24-25	0.001134606254686043
26-27	0.0017540711113458783
28-29	0.00435602709764836
30-31	0.008373319093247672
32-33	0.014634461174739221
34-35	2.176428810329594E-4
36-37	9.447871483685589E-4
38-39	0.0018676059534345466
40-41	0.0026899058623735734
42-43	3.4073309511721615E-4
44-45	5.30505428171329E-6
46-47	0.004087303512295201
48-49	0.0036161801142195154
50-51	8.856862425307471E-4
52-53	0.001289330223089292
54-55	0.0010331751991882743
56-57	0.00167204765901752
58-59	7.847232509850738E-4
60-61	4.6645391087720355E-4
62-63	0.0020441949602215408
64-65	5.175375886497508E-4
66-67	0.0022081499092233845
68-69	0.0019026224820183613
70-71	6.279786203422747E-4
72-73	8.347410827509422E-4
74-75	0.008336832325556792
76-77	0.0035147486821905556
78-79	0.006653011629324545
80-81	1.860019340819291E-4
82-83	0.0052493308392308384
84-85	0.003116085031812189
86-87	8.418162302382308E-4
88-89	0.003707559163330134
90-91	0.001026241625932934
92-93	8.675814244027268E-5
94-95	7.96186006211565E-4
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	607.0
20-21	1958.0
22-23	3690.0
24-25	8656.0
26-27	12975.0
28-29	27111.0
30-31	58541.0
32-33	76601.0
34-35	109702.0
36-37	160678.0
38-39	188876.0
40-41	194154.0
42-43	216197.0
44-45	252965.0
46-47	251128.0
48-49	232652.0
50-51	209096.0
52-53	200708.0
54-55	214720.0
56-57	226484.0
58-59	224474.0
60-61	218859.0
62-63	209396.0
64-65	220044.0
66-67	230112.0
68-69	237138.0
70-71	234754.0
72-73	220535.0
74-75	223893.0
76-77	222243.0
78-79	237048.0
80-81	242005.0
82-83	232168.0
84-85	234322.0
86-87	247048.0
88-89	273146.0
90-91	286857.0
92-93	309337.0
94-95	351740.0
96-97	746819.0
98-99	3991090.0
100-101	7930673.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	81.84167276073767
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.52823041035914	72.45298463331797
2	7.54512266291068	12.350109198351229
3	2.006490219972588	4.926435479418513
4	0.8415007813068892	2.7547932628649394
5	0.4068028399520575	1.6646712452747514
6	0.2101985654224739	1.0321801323649566
7	0.13146483062726655	0.7531511153419785
8	0.0912848317494501	0.5976722662445991
9	0.05573641447981103	0.4105405255242181
>10	0.17388812224373854	2.2473895646931625
>50	0.006410858845213075	0.35905676383473034
>100	0.0027923690332895684	0.39658284862328025
>500	6.42565750933863E-5	0.03859048214964123
>1k	1.2836522411371107E-5	0.01584248199598582
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	6.509373497836885E-5	0.0	0.0	0.0
3	0.0	6.509373497836885E-5	0.0	0.0	0.0
4	0.0	1.6523794263739784E-4	0.0	0.0	0.0
5	0.0	1.7525236340330076E-4	0.0	0.0	0.0
6	0.0	1.7525236340330076E-4	0.0	0.0	0.0
7	0.0	1.7525236340330076E-4	0.0	0.0	0.0
8	5.00721038295145E-6	2.503605191475725E-4	0.0	0.0	0.0
9	5.00721038295145E-6	6.259012978689312E-4	0.0	0.0	0.0
10-11	5.00721038295145E-6	6.259012978689312E-4	0.0	0.0	0.0
12-13	5.00721038295145E-6	6.409229290177856E-4	0.0	0.0	0.0
14-15	5.00721038295145E-6	8.762618170165037E-4	0.0	0.0	0.0
16-17	5.00721038295145E-6	9.038014741227368E-4	0.0	0.0	0.0
18-19	5.00721038295145E-6	9.989384713988143E-4	0.0	5.00721038295145E-6	0.0
20-21	7.510815574427175E-6	0.001119111520589649	0.0	1.2518025957378626E-5	0.0
22-23	1.2518025957378626E-5	0.0012543062009293384	0.0	3.755407787213588E-5	0.0
24-25	1.502163114885435E-5	0.0013794864605031244	0.0	6.759734016984457E-5	0.0
26-27	1.502163114885435E-5	0.0014320621695241148	0.0	1.6523794263739787E-4	0.0
28-29	1.502163114885435E-5	0.0014345657747155903	0.0	5.833400096138439E-4	0.0
30-31	1.7525236340330074E-5	0.001612321743310367	0.0	0.001802595737862522	0.0
32-33	2.00288415318058E-5	0.0016248397692677455	0.0	0.004261136035891684	0.0
34-35	2.00288415318058E-5	0.001930279602627784	0.0	0.007388138920044864	0.0
36-37	2.00288415318058E-5	0.002253244672328153	0.0	0.012660731453292742	0.0
38-39	2.00288415318058E-5	0.0023183384073065215	0.0	0.020987722320141004	0.0
40-41	2.00288415318058E-5	0.0026162674250921327	0.0	0.029384814132350584	0.0
42-43	2.00288415318058E-5	0.0027063972119852585	0.0	0.03704334241307483	0.0
44-45	2.00288415318058E-5	0.0030468875180259575	0.0	0.04600374539336645	0.0
46-47	2.00288415318058E-5	0.0032947444319820544	0.0	0.05601816615926935	0.0
48-49	2.00288415318058E-5	0.003307262457939433	0.0	0.06559195241147252	0.0
50-51	2.00288415318058E-5	0.0037478969716391603	0.0	0.07499048630027239	0.0
52-53	2.00288415318058E-5	0.003795465470277199	0.0	0.08381068738984138	0.0
54-55	2.00288415318058E-5	0.003840530363723762	0.0	0.09270098942477167	0.0
56-57	2.00288415318058E-5	0.003915638519468034	0.0	0.1007225404582599	0.0
58-59	2.00288415318058E-5	0.003928156545425413	0.0	0.10889681140842813	0.0
60-61	2.00288415318058E-5	0.003980732254446403	0.0	0.11685577231212946	0.0
62-63	2.00288415318058E-5	0.004043322384233296	0.0	0.1241537814452812	0.0
64-65	2.00288415318058E-5	0.004516503765422208	0.0	0.13112131469315813	0.0
66-67	2.00288415318058E-5	0.004546547027719916	0.0	0.13854700769107514	0.0
68-69	2.00288415318058E-5	0.004649194840570421	0.0	0.146193017945842	0.0
70-71	2.00288415318058E-5	0.004746835443037974	0.0	0.1531054718795065	0.0
72-73	2.00288415318058E-5	0.004789396731293062	0.0	0.15846569059445603	0.0
74-75	2.00288415318058E-5	0.004874519307803237	0.0	0.16280193478609198	0.0
76-77	2.00288415318058E-5	0.005057282486780964	0.0	0.1667401057522833	0.0
78-79	2.503605191475725E-5	0.005132390642525237	0.0	0.1694189633071623	0.0
80-81	2.503605191475725E-5	0.005139901458099663	0.0	0.1703052395449447	0.0
82-83	2.503605191475725E-5	0.005157426694439993	0.0	0.170843514661112	0.0
84-85	2.7539657106232974E-5	0.005194980772312129	0.0	0.17108386075949367	0.0
86-87	3.00432622977087E-5	0.00524755648133312	0.0	0.17117649415157826	0.0
88	3.00432622977087E-5	0.005447844896651178	0.0	0.1711865085723442	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	27795	0.0	52.255615	1
GTATCAA	50220	0.0	35.640644	1
TCAACGC	64220	0.0	27.371462	4
ATCAACG	64345	0.0	27.33722	3
TATCAAC	65925	0.0	26.854498	2
CAACGCA	65765	0.0	26.734604	5
AACGCAG	67385	0.0	26.152138	6
GTACATG	87085	0.0	23.252878	1
TACATGG	87930	0.0	22.641518	2
ACGCAGA	80140	0.0	21.964457	7
ACATGGG	89565	0.0	21.820175	3
TGGTATC	13460	0.0	21.660082	2
GTGGTAT	14080	0.0	21.600304	1
CGCAGAG	82420	0.0	21.351921	8
GCAGAGT	93245	0.0	18.694593	9
CATGGGG	57150	0.0	18.380653	4
GAGTACT	46350	0.0	18.16521	12-13
CAGAGTA	90225	0.0	17.434658	10-11
AGAGTAC	85040	0.0	16.972115	10-11
GTACTTT	51810	0.0	16.224678	14-15
>>END_MODULE