FastQCFastQC Report
Fri 27 May 2016
SRR2138720_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138720_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4169106
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA55650.049.2345541
GTATCAA100800.036.1751861
TCAACGC129300.027.5439014
TATCAAC131600.027.3396152
ATCAACG131200.027.2378853
CAACGCA132500.026.8786895
AACGCAG136750.026.191316
GTGGTAT26800.022.9538381
TGGTATC25750.022.7896292
GTACATG165800.022.554541
TACATGG164000.022.3578932
ACGCAGA160600.022.2009567
ACATGGG166350.021.6284523
CGCAGAG165150.021.5157898
CATGGGG119300.019.5060214
GCAGAGT186450.018.9275939
CTCACGA8401.6370905E-1118.2593594
GAGTACT100300.018.07699412-13
CAGAGTA180850.017.15279810-11
AGAGTAC168900.017.1084210-11
Produced by FastQC (version 0.11.5)