##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138720_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4169106
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.880662664849492	31.0	31.0	34.0	30.0	34.0
2	32.13105135729339	33.0	31.0	34.0	30.0	34.0
3	32.048206737847394	33.0	31.0	34.0	30.0	34.0
4	35.638144244833306	37.0	35.0	37.0	34.0	37.0
5	35.43828173234262	37.0	35.0	37.0	33.0	37.0
6	35.77413503038781	37.0	35.0	37.0	33.0	37.0
7	35.693573634251564	37.0	35.0	37.0	33.0	37.0
8	35.6723309505683	37.0	35.0	37.0	33.0	37.0
9	37.27046637816357	39.0	37.0	39.0	34.0	39.0
10-11	37.155407418281044	39.0	37.0	39.0	33.0	39.0
12-13	37.10610320294087	39.0	37.0	39.0	33.0	39.0
14-15	38.19700278668856	40.0	38.0	41.0	33.0	41.0
16-17	38.149399415606126	40.0	38.0	41.0	33.0	41.0
18-19	38.16762082806242	40.0	38.0	41.0	33.5	41.0
20-21	38.169398868169594	40.0	38.0	41.0	33.5	41.0
22-23	38.1059233334815	40.0	38.0	41.0	33.0	41.0
24-25	38.024302623653995	40.0	37.0	41.0	33.0	41.0
26-27	37.85854857464483	39.0	37.0	41.0	33.0	41.0
28-29	37.71353681927815	39.0	37.0	41.0	33.0	41.0
30-31	37.564024697587854	39.0	37.0	41.0	32.0	41.0
32-33	37.39668452306772	39.0	36.5	40.5	32.0	41.0
34-35	37.27305736877928	39.0	36.0	40.0	31.5	41.0
36-37	37.34264499326231	39.0	36.0	41.0	32.0	41.0
38-39	37.310179960387785	39.0	36.0	41.0	32.0	41.0
40-41	37.272783507127656	39.0	36.0	41.0	31.5	41.0
42-43	37.34150665019145	39.0	36.0	41.0	32.0	41.0
44-45	37.36467686424264	39.0	36.0	41.0	32.0	41.0
46-47	37.24481953615134	39.0	36.0	41.0	31.0	41.0
48-49	37.171432780394426	39.0	36.0	41.0	31.0	41.0
50-51	37.08271813459517	39.0	36.0	41.0	31.0	41.0
52-53	36.93828391085128	38.5	35.0	40.0	31.0	41.0
54-55	36.76898842142653	38.0	35.0	40.0	31.0	41.0
56-57	36.63420392196099	38.0	35.0	40.0	31.0	41.0
58-59	36.390366586438105	38.0	35.0	40.0	30.0	41.0
60-61	36.24056032660688	38.0	35.0	40.0	30.0	41.0
62-63	36.0000349980847	38.0	34.5	40.0	29.5	41.0
64-65	35.709659306147955	37.0	34.0	39.5	29.0	41.0
66-67	35.40542523602652	37.0	34.0	39.0	29.0	41.0
68-69	35.18467206997634	36.0	34.0	39.0	29.0	40.5
70-71	34.90146387879905	36.0	34.0	38.5	29.0	40.0
72-73	34.629419757832366	35.0	33.0	38.0	29.0	40.0
74-75	34.38031374896208	35.0	33.0	38.0	29.0	39.0
76-77	33.127244486475504	34.5	31.5	36.5	26.5	38.5
78-79	33.821751356587285	35.0	33.0	37.0	28.5	39.0
80-81	33.86117481518728	35.0	33.0	36.5	29.0	38.0
82-83	33.65699315724768	35.0	33.0	36.0	29.0	38.0
84-85	33.47791874136168	35.0	33.0	36.0	29.0	38.0
86-87	33.329406909796276	35.0	33.0	35.5	29.0	37.0
88-89	33.229457774727145	35.0	33.0	35.0	29.0	37.0
90-91	33.109892929199546	35.0	33.0	35.0	28.5	37.0
92-93	33.11440560797753	35.0	33.0	35.0	29.0	36.0
94-95	33.05166227688564	35.0	32.5	35.0	29.0	36.0
96-97	33.15162900513347	35.0	33.0	35.0	29.0	36.0
98-99	33.256247231192546	34.5	33.0	35.0	29.0	36.0
100	32.28234669256567	34.0	31.0	35.0	26.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	7.0
17	8.0
18	9.0
19	21.0
20	83.0
21	321.0
22	1174.0
23	3614.0
24	8628.0
25	17537.0
26	31029.0
27	47484.0
28	66574.0
29	86287.0
30	108736.0
31	134020.0
32	164765.0
33	205656.0
34	267029.0
35	358906.0
36	528017.0
37	938511.0
38	784167.0
39	401894.0
40	14629.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.734413564922555	19.734038904263887	13.452260508607841	24.07928702220572
2	15.81216692499543	21.252206108455866	39.360404844587784	23.57522212196092
3	18.777870230474257	25.772067297994123	30.135504098127676	25.31455837340394
4	13.112235572806256	17.26912196523667	37.63571854493505	31.982923917022017
5	13.364927636764332	37.94494551109998	35.01299799045647	13.677128861679218
6	31.106884785371253	36.93223439269714	18.091696397261188	13.869184424670422
7	27.434274878115357	32.40462103865912	22.313680678783413	17.847423404442104
8	25.737963966375528	35.54735715522705	20.665005878958222	18.04967299943921
9	26.37215268693096	15.897053229157523	20.704654666971766	37.02613941693975
10-11	25.22087469112083	26.251875102240145	28.102607609401154	20.424642597237874
12-13	25.66736130000053	23.903457959572147	28.367964259004207	22.061216481423116
14-15	22.77299977501172	25.172746867074142	26.1824357548117	25.871817603102436
16-17	22.27619062695935	28.203660449026724	26.8059027522927	22.71424617172123
18-19	22.26426621342149	27.381659976886795	28.626201294013065	21.727872515678648
20-21	23.313182695702903	26.570368031994107	28.406306131691668	21.710143140611326
22-23	22.906553474487676	26.45675251446541	28.301847410081134	22.33484660096578
24-25	22.539721126298527	26.58727620888806	28.511031756383094	22.36197090843032
26-27	22.506479401392028	26.78448698831517	28.37206488129031	22.336968729002496
28-29	22.48321389605833	26.90823276089955	28.370954873377265	22.237598469664853
30-31	22.79762554029842	26.7984283278542	28.126037646257707	22.277908485589673
32-33	22.5012326930738	27.048487054971226	28.101150167262134	22.34913008469284
34-35	22.637422228854163	27.020023814333122	28.138278416607676	22.20427554020504
36-37	22.686134599186104	26.914525387025108	28.082078702574176	22.31726131121461
38-39	22.42688836831479	27.16600874812552	28.13963537114972	22.267467512409976
40-41	22.780704906593456	26.976620740662582	27.916907644634065	22.3257667081099
42-43	22.602506358716507	27.058494154810457	27.740832260812837	22.598167225660198
44-45	22.65517205817551	27.260663135407366	27.562755013761368	22.521409792655756
46-47	22.801961019230113	27.081407550548892	27.337444137237267	22.77918729298373
48-49	22.718804904126362	27.24379142977787	27.295987764938275	22.74141590115749
50-51	22.587521699827672	27.3965731528798	27.240873618534284	22.77503152875824
52-53	22.850320392352618	27.34266298563529	26.93374786936807	22.873268752644023
54-55	22.896524013282704	27.133665803409286	27.107864396377984	22.861945786930026
56-57	22.805378665546446	27.36238685601078	27.038371355924962	22.79386312251782
58-59	22.772772396366044	27.44081282714638	27.03249960447181	22.753915172015766
60-61	22.827533865730054	27.329089798649704	27.090636215444945	22.7527401201753
62-63	22.891421013898118	27.461559297174183	26.943830052322088	22.70318963660561
64-65	22.756776743164433	27.463840852809362	27.064637029215348	22.714745374810853
66-67	22.906872643648978	27.474749879110625	26.797872229443993	22.820505247796405
68-69	22.778602562204377	27.42191771548978	26.962294851958525	22.83718487034732
70-71	22.780761257217602	27.110829992527552	27.04226571487946	23.066143035375383
72-73	22.905119151539598	27.206697019878995	26.96507762618239	22.923106202399023
74-75	22.5533076054698	27.44945073234648	26.959931481018586	23.037310181165136
76-77	22.75355441991266	27.340349645151136	26.881948497487507	23.024147437448693
78-79	22.715855971382386	27.428632645561517	26.806942906116447	23.048568476939643
80-81	22.756750906734673	27.36555359908248	26.785576409668593	23.092119084514252
82-83	22.783894859344194	27.289349107220367	26.980969184084618	22.945786849350814
84-85	22.930779665134736	27.315560909440613	26.7796718833303	22.97398754209436
86-87	22.621873629819923	27.408629635445227	26.940089184511724	23.029407550223134
88-89	22.686591688545967	27.396159264995333	26.92244763825912	22.99480140819958
90-91	22.726044536077325	27.35712954449996	27.01956423452112	22.89726168490159
92-93	22.835810563402088	27.408554413744486	26.878526158930143	22.877108863923283
94-95	22.566690107457585	27.552488495882997	26.967631744366138	22.913189652293276
96-97	22.572369336577445	27.56647285027506	27.12799446132561	22.733163351821887
98-99	23.715555789217955	29.6311316328882	27.194685273354267	19.458627304539576
100	30.64151630574988	41.27228857155865	28.08619512269147	0.0
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1935.0
1	1732.5
2	2036.5
3	2668.5
4	3258.0
5	4691.5
6	6097.5
7	7353.0
8	8316.5
9	8404.5
10	7640.0
11	6679.0
12	6813.5
13	8405.0
14	12510.0
15	18279.0
16	23102.0
17	25797.0
18	26233.5
19	25139.0
20	23262.5
21	21273.5
22	20207.0
23	20713.5
24	23693.5
25	29184.0
26	37080.5
27	44987.0
28	51322.5
29	58931.5
30	65890.0
31	71710.5
32	79781.5
33	89254.5
34	98595.0
35	107464.0
36	117232.0
37	124910.0
38	129289.5
39	133488.5
40	139451.5
41	145411.0
42	150110.0
43	155672.0
44	161717.5
45	167313.0
46	171213.5
47	175057.5
48	179443.0
49	182681.0
50	183956.0
51	182760.0
52	178998.5
53	175084.5
54	170549.5
55	163122.5
56	153419.5
57	141711.5
58	128584.5
59	113537.5
60	97396.5
61	80831.0
62	64880.5
63	50899.5
64	39226.5
65	30774.5
66	23997.5
67	18067.0
68	13311.0
69	9668.0
70	6739.5
71	4461.5
72	3006.5
73	2009.0
74	1322.5
75	897.5
76	608.5
77	402.0
78	270.0
79	180.0
80	120.5
81	82.5
82	58.5
83	31.5
84	17.0
85	11.5
86	9.0
87	8.5
88	8.0
89	5.0
90	2.0
91	1.5
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	1.679017036266288E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0014391574596568184
20-21	0.002830492366545883
22-23	0.0048100763542644025
24-25	9.958904520502745E-4
26-27	0.0015366170439401645
28-29	0.004085212731445325
30-31	0.00746387424672053
32-33	0.013043947716958895
34-35	2.0623679477934604E-4
36-37	8.669814745596498E-4
38-39	0.0015893781243908921
40-41	0.0026378303695898967
42-43	3.647373903366727E-4
44-45	2.546396943814388E-5
46-47	0.0035501997600581358
48-49	0.003218823049128268
50-51	7.551741352063772E-4
52-53	0.0011661705973541332
54-55	0.0011937417006125657
56-57	0.0014840813721816368
58-59	8.216891394003981E-4
60-61	3.8109519419199633E-4
62-63	0.0017018046422656481
64-65	4.346554877138831E-4
66-67	0.0016015473592247218
68-69	0.0013717226824037053
70-71	5.448787077293315E-4
72-73	5.068912635293886E-4
74-75	0.007292700318634907
76-77	0.00275203935606075
78-79	0.005524826385542942
80-81	1.3058855773263005E-4
82-83	0.004315605545021974
84-85	0.002547529562730891
86-87	6.521049346668078E-4
88-89	0.0034259888522517835
90-91	9.274176216838801E-4
92-93	9.108571438981225E-5
94-95	7.090027111890517E-4
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	141.0
20-21	468.0
22-23	890.0
24-25	1986.0
26-27	3007.0
28-29	6270.0
30-31	12679.0
32-33	16921.0
34-35	24091.0
36-37	34310.0
38-39	40000.0
40-41	41852.0
42-43	46136.0
44-45	53758.0
46-47	52848.0
48-49	48628.0
50-51	44308.0
52-53	43402.0
54-55	46757.0
56-57	48452.0
58-59	47808.0
60-61	46923.0
62-63	45068.0
64-65	47068.0
66-67	49525.0
68-69	49881.0
70-71	49278.0
72-73	46434.0
74-75	47497.0
76-77	46951.0
78-79	50118.0
80-81	51094.0
82-83	48758.0
84-85	49353.0
86-87	52163.0
88-89	56201.0
90-91	58354.0
92-93	62608.0
94-95	73070.0
96-97	153290.0
98-99	823335.0
100-101	1647425.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	88.52387609659826
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.0554446273175	81.49104774205914
2	5.844989792811557	10.348423044094634
3	1.2292541419468925	3.264550240588107
4	0.4304673195051226	1.5242654262202497
5	0.18425609875508758	0.8155532028118972
6	0.08762558369177358	0.46541737881735984
7	0.045444993370497073	0.2816076873638428
8	0.030368693581001333	0.21506835742240962
9	0.017920494273977513	0.14277524532294458
>10	0.07042101449119109	0.9700817177550041
>50	0.0022917265743029754	0.1339910153511419
>100	0.0014768949093131264	0.31552774738004935
>500	3.861877176944983E-5	0.031691194813217076
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	9.59438306437879E-5	0.0	0.0	0.0
3	0.0	9.59438306437879E-5	0.0	0.0	0.0
4	0.0	1.6790170362662884E-4	0.0	0.0	0.0
5	0.0	1.6790170362662884E-4	0.0	0.0	0.0
6	0.0	1.6790170362662884E-4	0.0	0.0	0.0
7	0.0	1.6790170362662884E-4	0.0	0.0	0.0
8	0.0	1.918876612875758E-4	0.0	0.0	0.0
9	0.0	3.837753225751516E-4	0.0	0.0	0.0
10-11	0.0	3.837753225751516E-4	0.0	2.3985957660946974E-5	0.0
12-13	2.3985957660946974E-5	3.837753225751516E-4	0.0	2.3985957660946974E-5	0.0
14-15	2.3985957660946974E-5	4.5573319555799254E-4	0.0	2.3985957660946974E-5	0.0
16-17	3.597893649142046E-5	4.6772617438846603E-4	0.0	3.597893649142046E-5	2.3985957660946974E-5
18-19	4.797191532189395E-5	5.037051108798865E-4	0.0	7.195787298284093E-5	2.3985957660946974E-5
20-21	4.797191532189395E-5	5.756629838627274E-4	0.0	7.195787298284093E-5	2.3985957660946974E-5
22-23	7.195787298284093E-5	6.596138356760419E-4	0.0	7.195787298284093E-5	2.3985957660946974E-5
24-25	8.395085181331442E-5	7.435646874893562E-4	0.0	7.195787298284093E-5	2.3985957660946974E-5
26-27	9.59438306437879E-5	7.675506451503032E-4	0.0	9.594383064378791E-5	7.195787298284093E-5
28-29	9.59438306437879E-5	7.675506451503032E-4	0.0	4.5573319555799254E-4	7.195787298284093E-5
30-31	1.5590872479615534E-4	8.275155393026707E-4	0.0	0.0018709046975538641	7.195787298284093E-5
32-33	1.918876612875758E-4	8.395085181331441E-4	0.0	0.00412558471768288	7.195787298284093E-5
34-35	2.3985957660946973E-4	0.0010553821370816669	0.0	0.007519597726706877	7.195787298284093E-5
36-37	2.878314919313637E-4	0.0012712557560301896	0.0	0.012772522454454264	9.59438306437879E-5
38-39	2.878314919313637E-4	0.0012952417136911367	0.0	0.021407467212395177	9.59438306437879E-5
40-41	3.118174495923107E-4	0.0014151715019958715	0.0	0.030666046869520708	9.59438306437879E-5
42-43	3.118174495923107E-4	0.0014631434173177655	0.0	0.03922903375447878	9.59438306437879E-5
44-45	3.3580340725325767E-4	0.0016670240574358148	0.0	0.04903929043780609	1.1992978830473487E-4
46-47	3.5978936491420466E-4	0.001846918739892917	0.0	0.059329266274352345	1.4391574596568185E-4
48-49	3.5978936491420466E-4	0.0018589117187233907	0.0	0.06912752997884919	1.4391574596568185E-4
50-51	3.5978936491420466E-4	0.0021587361894852275	0.0	0.07856600431843182	1.4391574596568185E-4
52-53	3.837753225751516E-4	0.002182722147146175	0.0	0.0879804927003535	1.5590872479615534E-4
54-55	3.957683014056251E-4	0.002182722147146175	0.0	0.09629162702987162	1.6790170362662884E-4
56-57	4.0776128023609857E-4	0.002182722147146175	0.0	0.10575408732711522	1.6790170362662884E-4
58-59	4.0776128023609857E-4	0.0021947151259766482	0.0	0.11467686357698748	1.6790170362662884E-4
60-61	4.3174723789704555E-4	0.002266672998959489	0.0	0.12304796280065798	1.6790170362662884E-4
62-63	4.3174723789704555E-4	0.002314644914281383	0.0	0.13121518138421043	1.918876612875758E-4
64-65	4.3174723789704555E-4	0.002638455342704167	0.0	0.13830303187302026	1.918876612875758E-4
66-67	4.3174723789704555E-4	0.002698420236856535	0.0	0.14573867874791382	1.918876612875758E-4
68-69	4.3174723789704555E-4	0.002734399173347955	0.0	0.15372600264900915	1.918876612875758E-4
70-71	4.5573319555799254E-4	0.002770378109839376	0.0	0.16038210589992197	1.918876612875758E-4
72-73	4.5573319555799254E-4	0.0027943640675003227	0.0	0.16594684807726165	2.1587361894852278E-4
74-75	4.5573319555799254E-4	0.0028543289616526904	0.0	0.17055215194816348	2.1587361894852278E-4
76-77	4.797191532189395E-4	0.002950272792296478	0.0	0.1749655681577777	2.2786659777899627E-4
78-79	4.797191532189395E-4	0.002998244707618372	0.0	0.17785587605592185	2.3985957660946976E-4
80-81	4.797191532189395E-4	0.002998244707618372	0.0	0.1788632862776816	2.518525554399432E-4
82-83	4.797191532189395E-4	0.0030102376864488455	0.0	0.17934300543090054	3.118174495923107E-4
84-85	4.91712132049413E-4	0.0030702025806012127	0.0	0.17964282990166236	3.118174495923107E-4
86-87	5.037051108798865E-4	0.00309418853826216	0.0	0.17978674564762803	3.118174495923107E-4
88	5.037051108798865E-4	0.0031661464112450006	0.0	0.17982272458411946	3.118174495923107E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5565	0.0	49.234554	1
GTATCAA	10080	0.0	36.175186	1
TCAACGC	12930	0.0	27.543901	4
TATCAAC	13160	0.0	27.339615	2
ATCAACG	13120	0.0	27.237885	3
CAACGCA	13250	0.0	26.878689	5
AACGCAG	13675	0.0	26.19131	6
GTGGTAT	2680	0.0	22.953838	1
TGGTATC	2575	0.0	22.789629	2
GTACATG	16580	0.0	22.55454	1
TACATGG	16400	0.0	22.357893	2
ACGCAGA	16060	0.0	22.200956	7
ACATGGG	16635	0.0	21.628452	3
CGCAGAG	16515	0.0	21.515789	8
CATGGGG	11930	0.0	19.506021	4
GCAGAGT	18645	0.0	18.927593	9
CTCACGA	840	1.6370905E-11	18.25935	94
GAGTACT	10030	0.0	18.076994	12-13
CAGAGTA	18085	0.0	17.152798	10-11
AGAGTAC	16890	0.0	17.10842	10-11
>>END_MODULE