FastQCFastQC Report
Fri 27 May 2016
SRR2138719_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138719_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6602773
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA78700.049.3458441
GTATCAA135950.033.4867821
TCAACGC168550.026.404894
ATCAACG172550.025.7927823
TATCAAC175800.025.5944022
CAACGCA174700.025.4053025
AACGCAG176600.025.2243716
TACATGG209050.021.3479182
GTACATG212150.021.3436321
ACGCAGA210350.021.1771987
CGCAGAG217450.020.4669788
ACATGGG212650.020.4493833
GTGGTAT41100.019.9499861
TGGTATC38450.018.990882
CATGGGG168200.017.922844
GCAGAGT249850.017.5679599
GAGTACT141200.017.0313512-13
AGAGTAC223550.016.91583410-11
CAGAGTA241800.016.89596710-11
GAGTACA151600.015.8221861
Produced by FastQC (version 0.11.5)