##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138719_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6602773
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.738160618273564	31.0	31.0	34.0	30.0	34.0
2	32.009288824559015	31.0	31.0	34.0	30.0	34.0
3	31.915978937940164	31.0	31.0	34.0	30.0	34.0
4	35.77983174644956	37.0	35.0	37.0	35.0	37.0
5	35.55820047122626	37.0	35.0	37.0	33.0	37.0
6	35.64339149626983	37.0	35.0	37.0	33.0	37.0
7	35.55737203141771	37.0	35.0	37.0	33.0	37.0
8	35.542078457036155	37.0	35.0	37.0	33.0	37.0
9	37.2598845969716	39.0	37.0	39.0	33.0	39.0
10-11	37.13245147152568	39.0	37.0	39.0	33.0	39.0
12-13	37.092273428149056	39.0	37.0	39.0	33.0	39.0
14-15	38.264420115609	40.0	38.0	41.0	33.0	41.0
16-17	38.21769989669492	40.0	38.0	41.0	33.0	41.0
18-19	38.21758827692547	40.0	37.5	41.0	33.0	41.0
20-21	38.21398572710433	40.0	38.0	41.0	33.0	41.0
22-23	38.14583551742453	40.0	37.0	41.0	33.0	41.0
24-25	38.065308125898426	40.0	37.0	41.0	33.0	41.0
26-27	37.8976484011511	40.0	37.0	41.0	33.0	41.0
28-29	37.7443872688429	40.0	37.0	41.0	32.0	41.0
30-31	37.582258489993805	39.5	37.0	41.0	32.0	41.0
32-33	37.41303103998681	39.0	36.0	41.0	31.5	41.0
34-35	37.28408281108585	39.0	36.0	41.0	31.0	41.0
36-37	37.37211426165803	39.0	36.0	41.0	31.0	41.0
38-39	37.33985102581489	39.0	36.0	41.0	31.0	41.0
40-41	37.30091884097337	39.0	36.0	41.0	31.0	41.0
42-43	37.38749949694125	39.0	36.0	41.0	31.0	41.0
44-45	37.424546401915734	39.0	36.0	41.0	31.0	41.0
46-47	37.302967661017746	39.0	36.0	41.0	31.0	41.0
48-49	37.236185322051696	39.0	36.0	41.0	31.0	41.0
50-51	37.14057482754195	39.0	36.0	41.0	31.0	41.0
52-53	36.991082231828926	39.0	35.0	41.0	31.0	41.0
54-55	36.815467601346754	39.0	35.0	40.0	31.0	41.0
56-57	36.674329101314896	38.5	35.0	40.0	30.0	41.0
58-59	36.41330651218421	38.0	35.0	40.0	30.0	41.0
60-61	36.24795245563209	38.0	34.5	40.0	30.0	41.0
62-63	35.9849591527382	37.5	34.0	40.0	29.0	41.0
64-65	35.67909535190018	37.0	34.0	40.0	29.0	41.0
66-67	35.34765649347388	36.5	34.0	39.0	29.0	41.0
68-69	35.11112622646786	36.0	34.0	39.0	29.0	41.0
70-71	34.78987854681992	35.5	33.0	39.0	28.0	40.0
72-73	34.49426109767012	35.0	33.0	38.0	28.0	40.0
74-75	34.21120249522148	35.0	33.0	37.0	28.0	39.5
76-77	32.85518732678792	34.0	31.5	36.0	26.5	39.0
78-79	33.61425202747624	35.0	32.5	36.0	28.0	39.0
80-81	33.65341422865574	35.0	33.0	36.0	29.0	38.5
82-83	33.42738494362938	35.0	33.0	36.0	28.5	37.0
84-85	33.22700637739568	35.0	33.0	35.0	28.0	37.0
86-87	33.05815789932488	35.0	32.0	35.0	28.0	36.5
88-89	32.945626013633344	35.0	32.0	35.0	28.0	36.0
90-91	32.811405343864486	34.0	32.0	35.0	27.5	36.0
92-93	32.806848361852815	34.0	32.0	35.0	28.5	36.0
94-95	32.746318604705635	34.0	32.0	35.0	28.0	35.0
96-97	32.85026036627514	34.0	32.0	35.0	29.0	35.0
98-99	32.978037131337494	34.0	32.0	35.0	29.0	35.0
100	31.930357123369994	34.0	31.0	35.0	26.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	3.0
19	17.0
20	65.0
21	325.0
22	1506.0
23	5190.0
24	13271.0
25	28154.0
26	49881.0
27	79077.0
28	111386.0
29	147004.0
30	184230.0
31	225357.0
32	274531.0
33	340169.0
34	437051.0
35	579627.0
36	837143.0
37	1267012.0
38	1344977.0
39	654349.0
40	22448.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.18524550215493	19.87159031516001	13.553547880564725	24.389616302120338
2	15.850325310290087	21.127199132849185	39.26088326828743	23.7615922885733
3	18.75086895401109	25.337366188654876	30.165115650016975	25.746649207317056
4	13.241845509454892	17.218356590480997	37.47155929788894	32.06823860217518
5	13.542476774530943	37.67336844686316	35.17658414123884	13.607570637367058
6	31.511745141018782	36.810973207771944	17.895799234654895	13.781482416554377
7	27.617199622037592	32.10357224154154	22.34692908570384	17.932299050717024
8	25.626005922057292	35.856919509424294	20.54239938280477	17.97467518571364
9	26.069607421003266	16.045834076076822	20.731107975391552	37.153450527528356
10-11	25.10144601366729	26.17719100747519	28.250759188601513	20.470603790256003
12-13	25.522708413571088	23.736663368557423	28.55008797061477	22.19054024725672
14-15	22.595892665096923	25.122179726608806	26.104683592787453	26.17724401550682
16-17	22.372251779668936	28.142781828180375	26.80816075306542	22.676805639085277
18-19	22.37082219704383	27.4509769770167	28.42252436630493	21.755676459634543
20-21	23.147087343201274	26.605656189537697	28.319495129996515	21.927761337264513
22-23	22.851733599378676	26.515815402867975	28.17567263577121	22.456778361982135
24-25	22.59715182527672	26.654576540528947	28.284485950948167	22.463785683246172
26-27	22.57628010198977	26.792074570166474	28.21301127526627	22.418634052577485
28-29	22.523734896793698	26.958532459571234	28.156714912622398	22.36101773101267
30-31	22.824272148199963	26.774372211625902	28.013974681604974	22.387380958569167
32-33	22.578658409839104	26.918592291291755	28.010620799512896	22.492128499356248
34-35	22.714304625727806	26.988107982366667	28.01110768470275	22.286479707202776
36-37	22.692835782361197	26.95302886928018	27.9617936720038	22.39234167635483
38-39	22.53346528893512	27.079569631440613	28.028422193562168	22.358542886062104
40-41	22.850729385563582	26.89349525978671	27.84663580526069	22.409139549389018
42-43	22.628056564567995	27.042858864009578	27.66829900582892	22.660785565593503
44-45	22.711706991712795	27.145491871249188	27.50233286216511	22.64046827487291
46-47	22.82468668976287	27.067430748816424	27.328212024298367	22.77967053712234
48-49	22.70818732586638	27.24906938930276	27.258425883701232	22.784317401129627
50-51	22.703056260491167	27.318845912124807	27.19143543010795	22.786662397276075
52-53	22.873449366386154	27.24823079400566	27.00760118827987	22.87071865132831
54-55	22.833441622013112	27.089203675085177	27.154710422068394	22.92264428083331
56-57	22.80741498837712	27.24302798406396	27.108276247488565	22.841280780070356
58-59	22.805549010342016	27.361217244822374	27.089234331518174	22.743999413317432
60-61	22.918903226498045	27.203573411208527	27.13803404391867	22.739489318374766
62-63	22.95426513315856	27.3182190596098	27.028516164057237	22.698999643174403
64-65	22.794663720751114	27.28571462007213	27.09133568476063	22.828285974416122
66-67	22.867242088107215	27.301973846895766	26.92983834754252	22.900945717454494
68-69	22.799892200359356	27.269350756872594	27.030722897583313	22.900034145184733
70-71	22.795873366076446	27.022044150582047	27.119433071356543	23.06264941198496
72-73	22.893325926118262	27.116570333875366	26.99697824049728	22.993125499509087
74-75	22.661079476855733	27.268331688832077	27.077288601870485	22.9933002324417
76-77	22.829995830831717	27.118234306866242	27.015252036149224	23.03651782615282
78-79	22.74171613031909	27.095189022021355	27.017558831686973	23.14553601597259
80-81	22.808078984048567	27.161760663241573	26.957178143791015	23.072982208918837
82-83	22.874192366910577	27.120129972854745	27.11003407927242	22.895643580962258
84-85	22.93958928833897	27.109045894324087	27.012410059239116	22.93895475809783
86-87	22.725914868944752	27.15936797227204	27.08728179709344	23.027435361689765
88-89	22.809942945266258	27.05734133166183	27.131197895324792	23.001517827747115
90-91	22.838678694828452	27.062726934013824	27.152610817612715	22.945983553545005
92-93	22.88676678009944	27.003813154102673	27.13532366484633	22.97409640095156
94-95	22.71766347449972	27.06284008211517	27.235342628959046	22.98415381442607
96-97	22.684971335228855	27.214155865590424	27.298257071329758	22.802615727850966
98-99	23.833590270180974	29.190569734824678	27.461668587202514	19.51417140779183
100	30.831943205255907	40.72703353504459	28.441023259699506	0.0
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1755.0
1	1527.0
2	1973.0
3	2859.0
4	3710.0
5	5571.0
6	7372.5
7	9084.5
8	10561.0
9	10756.0
10	9616.0
11	8377.5
12	8805.5
13	11217.0
14	17022.5
15	24812.0
16	30924.0
17	34372.5
18	35349.5
19	34393.5
20	32462.5
21	30388.0
22	29409.0
23	31132.0
24	36057.0
25	43711.0
26	54599.0
27	66588.5
28	78116.0
29	91417.5
30	104447.5
31	115997.5
32	128484.5
33	142104.5
34	154698.0
35	166594.5
36	180630.0
37	193889.5
38	204109.5
39	212990.0
40	223010.0
41	233967.5
42	241951.0
43	249768.0
44	259541.5
45	267658.5
46	274173.0
47	280747.0
48	287206.0
49	292240.5
50	293577.5
51	291676.5
52	286010.5
53	277184.0
54	266393.5
55	251991.0
56	234869.0
57	216168.5
58	195794.5
59	171736.5
60	146421.5
61	121885.5
62	99487.0
63	79449.0
64	61487.5
65	47851.5
66	36888.5
67	27794.0
68	20708.0
69	15346.0
70	11020.5
71	7734.0
72	5413.5
73	3686.0
74	2484.0
75	1676.5
76	1131.5
77	753.5
78	517.0
79	360.5
80	235.0
81	152.5
82	113.0
83	81.0
84	52.5
85	36.0
86	26.5
87	18.0
88	11.5
89	6.0
90	4.5
91	4.0
92	3.5
93	2.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	1.0601606325100076E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0016659667082300118
20-21	0.0034532468244517743
22-23	0.004839662144624707
24-25	0.0010984934200622928
26-27	0.0017659926360380888
28-29	0.004369277034086201
30-31	0.007963706169030272
32-33	0.01451863522085245
34-35	1.3773632492549996E-4
36-37	8.005517032853641E-4
38-39	0.0017306455812466314
40-41	0.0026702508062434694
42-43	2.529771457284335E-4
44-45	1.5985348469007248E-5
46-47	0.003909016863644427
48-49	0.003662171480892846
50-51	8.535393583986076E-4
52-53	0.0010554186070803008
54-55	9.230988377169377E-4
56-57	0.0014571249553219775
58-59	6.595795995848467E-4
60-61	4.5690193425919237E-4
62-63	0.0018854835553816325
64-65	5.581172939304925E-4
66-67	0.0021343656122687713
68-69	0.0017109293333470207
70-71	6.753303420576321E-4
72-73	7.323464093626209E-4
74-75	0.008398594397356497
76-77	0.003246818973119175
78-79	0.006290993787494794
80-81	1.2092341960144446E-4
82-83	0.005303646564173868
84-85	0.003193353497575028
86-87	7.507818705846624E-4
88-89	0.0039618277892505705
90-91	8.677459595716058E-4
92-93	4.4876455119057237E-5
94-95	6.207570108756629E-4
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	173.0
20-21	656.0
22-23	1276.0
24-25	2890.0
26-27	4450.0
28-29	9270.0
30-31	18022.0
32-33	24334.0
34-35	34485.0
36-37	49847.0
38-39	58163.0
40-41	58933.0
42-43	65627.0
44-45	77123.0
46-47	76329.0
48-49	71075.0
50-51	65160.0
52-53	64058.0
54-55	69294.0
56-57	72333.0
58-59	71214.0
60-61	69366.0
62-63	67001.0
64-65	71233.0
66-67	75021.0
68-69	75799.0
70-71	74775.0
72-73	71527.0
74-75	72925.0
76-77	72410.0
78-79	76409.0
80-81	78952.0
82-83	76369.0
84-85	78073.0
86-87	81715.0
88-89	90527.0
90-91	94755.0
92-93	103236.0
94-95	120125.0
96-97	254156.0
98-99	1346258.0
100-101	2657429.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.20807233877123
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.36158795548086	76.99536723156852
2	6.621741267494887	11.284516178586621
3	1.6566701413001563	4.234850077241583
4	0.5955923521542277	2.0299710490710536
5	0.2832744379730242	1.2068634401265106
6	0.1450821390888426	0.7417301641527462
7	0.08782639237887478	0.5238462316550727
8	0.05990573147824469	0.4083561521044223
9	0.03922452119035386	0.3008021255137204
>10	0.1450253940257385	1.9368131396431072
>50	0.0032751995966297407	0.18681719335266075
>100	7.057531856352341E-4	0.0974297633339678
>500	8.871465254683322E-5	0.052637253649985574
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.5435455679000326E-5
2	0.0	4.5435455679000326E-5	0.0	0.0	4.5435455679000326E-5
3	0.0	4.5435455679000326E-5	0.0	0.0	4.5435455679000326E-5
4	0.0	1.968869746090014E-4	0.0	1.5145151893000107E-5	4.5435455679000326E-5
5	0.0	2.1203212650200152E-4	0.0	3.0290303786000214E-5	4.5435455679000326E-5
6	1.5145151893000107E-5	2.2717727839500162E-4	0.0	4.5435455679000326E-5	6.058060757200043E-5
7	1.5145151893000107E-5	2.423224302880017E-4	0.0	4.5435455679000326E-5	6.058060757200043E-5
8	1.5145151893000107E-5	2.574675821810018E-4	0.0	4.5435455679000326E-5	6.058060757200043E-5
9	1.5145151893000107E-5	4.5435455679000323E-4	0.0	9.087091135800065E-5	7.572575946500054E-5
10-11	1.5145151893000107E-5	4.5435455679000323E-4	0.0	1.0601606325100075E-4	7.572575946500054E-5
12-13	1.5145151893000107E-5	4.5435455679000323E-4	0.0	1.2116121514400086E-4	7.572575946500054E-5
14-15	1.5145151893000107E-5	6.285238035595045E-4	0.0	1.3630636703700096E-4	1.1358863919750081E-4
16-17	3.0290303786000214E-5	6.663866832920047E-4	0.0	1.5145151893000109E-4	1.6659667082300118E-4
18-19	3.0290303786000214E-5	7.421124427570053E-4	0.0	1.7416924676950124E-4	1.817418227160013E-4
20-21	3.0290303786000214E-5	8.178382022220058E-4	0.0	2.0445955055550146E-4	2.1960470244850158E-4
22-23	3.786287973250027E-5	8.784188097940063E-4	0.0	2.423224302880017E-4	2.650401581275019E-4
24-25	4.5435455679000326E-5	0.0010222977527775073	0.0	3.104756138065022E-4	2.8775788596700205E-4
26-27	4.5435455679000326E-5	0.0010753057844030077	0.0	3.7105622137850264E-4	3.0290303786000217E-4
28-29	4.5435455679000326E-5	0.001098023512242508	0.0	9.465719933125068E-4	3.4076591759250245E-4
30-31	4.5435455679000326E-5	0.0012419024552260088	0.0	0.0020445955055550143	3.634836454320026E-4
32-33	4.5435455679000326E-5	0.001249475031172509	0.0	0.004399666624916532	3.634836454320026E-4
34-35	6.058060757200043E-5	0.0014993700374070105	0.0	0.007814898376788055	3.786287973250027E-4
36-37	6.058060757200043E-5	0.0017341198917485124	0.0	0.01248717773577859	3.786287973250027E-4
38-39	6.058060757200043E-5	0.0018098456512135128	0.0	0.019840148979830143	3.8620137327150276E-4
40-41	6.058060757200043E-5	0.001999160049876014	0.0	0.027458160382009194	4.2406425300400304E-4
42-43	6.058060757200043E-5	0.0020597406574480144	0.0	0.03545480058151325	4.2406425300400304E-4
44-45	6.058060757200043E-5	0.0022944905117895163	0.0	0.044223843527560314	4.316368289505031E-4
46-47	7.572575946500054E-5	0.0024686597585590175	0.0	0.05384101497961538	4.4678198084350314E-4
48-49	7.572575946500054E-5	0.0024838049104520176	0.0	0.06298111414704095	4.5435455679000323E-4
50-51	9.087091135800065E-5	0.0027412724926330198	0.0	0.07142453632738852	4.5435455679000323E-4
52-53	9.087091135800065E-5	0.0027715627964190197	0.0	0.07953476516609007	4.770722846295034E-4
54-55	9.087091135800065E-5	0.00278670794831202	0.0	0.08847040478296012	5.149351643620036E-4
56-57	9.087091135800065E-5	0.0028169982520980202	0.0	0.09598240012188819	5.45225468148004E-4
58-59	9.087091135800065E-5	0.00284728855588402	0.0	0.10343381485324424	5.60370620041004E-4
60-61	9.844348730450071E-5	0.0029154417394025205	0.0	0.11071863291377729	5.67943195987504E-4
62-63	1.2116121514400086E-4	0.002976022346974521	0.0	0.11844266037920734	5.755157719340041E-4
64-65	1.2116121514400086E-4	0.0033016431126740233	0.0	0.12530341418673638	5.830883478805042E-4
66-67	1.2116121514400086E-4	0.0033319334164600236	0.0	0.13286841755728995	5.982334997735043E-4
68-69	1.2116121514400086E-4	0.0034682397834970248	0.0	0.13981246970023048	6.058060757200043E-4
70-71	1.2116121514400086E-4	0.0035515381189085253	0.0	0.14674137669127804	6.360963795060045E-4
72-73	1.3630636703700096E-4	0.0035591106948550254	0.0	0.1519285912146306	6.815318351850049E-4
74-75	1.3630636703700096E-4	0.003612118726480526	0.0	0.15640398359901211	6.815318351850049E-4
76-77	1.3630636703700096E-4	0.0037181347897315264	0.0	0.16037201339497814	6.89104411131505E-4
78-79	1.3630636703700096E-4	0.003755997669464027	0.0	0.16293911664084165	6.96676987078005E-4
80-81	1.3630636703700096E-4	0.003755997669464027	0.0	0.16371909196333118	7.11822138971005E-4
82-83	1.3630636703700096E-4	0.003755997669464027	0.0	0.16433247061499767	7.269672908640052E-4
84-85	1.3630636703700096E-4	0.0037711428213570266	0.0	0.16450663986176717	7.345398668105053E-4
86-87	1.3630636703700096E-4	0.0038468685808220274	0.0	0.16465809138069717	7.421124427570053E-4
88	1.3630636703700096E-4	0.004028610403538029	0.0	0.16467323653259017	7.421124427570053E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7870	0.0	49.345844	1
GTATCAA	13595	0.0	33.486782	1
TCAACGC	16855	0.0	26.40489	4
ATCAACG	17255	0.0	25.792782	3
TATCAAC	17580	0.0	25.594402	2
CAACGCA	17470	0.0	25.405302	5
AACGCAG	17660	0.0	25.224371	6
TACATGG	20905	0.0	21.347918	2
GTACATG	21215	0.0	21.343632	1
ACGCAGA	21035	0.0	21.177198	7
CGCAGAG	21745	0.0	20.466978	8
ACATGGG	21265	0.0	20.449383	3
GTGGTAT	4110	0.0	19.949986	1
TGGTATC	3845	0.0	18.99088	2
CATGGGG	16820	0.0	17.92284	4
GCAGAGT	24985	0.0	17.567959	9
GAGTACT	14120	0.0	17.03135	12-13
AGAGTAC	22355	0.0	16.915834	10-11
CAGAGTA	24180	0.0	16.895967	10-11
GAGTACA	15160	0.0	15.822186	1
>>END_MODULE