FastQCFastQC Report
Fri 27 May 2016
SRR2138718_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138718_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13810415
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA147650.045.4593771
GTATCAA251050.032.484831
TCAACGC295100.026.79544
ATCAACG301600.026.2313923
CAACGCA307050.025.8055215
TATCAAC316200.025.174492
AACGCAG315550.025.097516
GTACATG382550.021.9559361
TACATGG387450.021.4369282
ACGCAGA377350.020.9764237
CGCAGAG384950.020.5939738
ACATGGG400700.020.3120883
CATGGGG297950.018.6387654
GTGGTAT81000.018.3196741
GAGTACT251850.017.33727512-13
GCAGAGT461950.017.0732679
TGGTATC82200.016.4201132
CAGAGTA440500.016.3864410-11
AGAGTAC400850.016.1563710-11
ATGGGGG189800.015.3793545
Produced by FastQC (version 0.11.5)