##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138718_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	13810415
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.763980734829474	31.0	31.0	34.0	30.0	34.0
2	32.036922641354366	33.0	31.0	34.0	30.0	34.0
3	31.94760229869993	31.0	31.0	34.0	30.0	34.0
4	35.799454614506516	37.0	35.0	37.0	35.0	37.0
5	35.57955296781451	37.0	35.0	37.0	33.0	37.0
6	35.66283243479649	37.0	35.0	37.0	33.0	37.0
7	35.58132221225792	37.0	35.0	37.0	33.0	37.0
8	35.56555939846847	37.0	35.0	37.0	33.0	37.0
9	37.29017049813492	39.0	37.0	39.0	34.0	39.0
10-11	37.164756127893334	39.0	37.0	39.0	33.0	39.0
12-13	37.12641014046283	39.0	37.0	39.0	33.0	39.0
14-15	38.31966074154904	40.0	38.0	41.0	33.0	41.0
16-17	38.27233587839323	40.0	38.0	41.0	33.0	41.0
18-19	38.27133630669317	40.0	38.0	41.0	33.5	41.0
20-21	38.26982846735207	40.0	38.0	41.0	33.0	41.0
22-23	38.20134773813088	40.0	38.0	41.0	33.0	41.0
24-25	38.12228864448526	40.0	37.0	41.0	33.0	41.0
26-27	37.95744165794071	40.0	37.0	41.0	33.0	41.0
28-29	37.809501484727804	40.0	37.0	41.0	32.5	41.0
30-31	37.65580809334296	40.0	37.0	41.0	32.0	41.0
32-33	37.497256907123614	39.0	36.5	41.0	32.0	41.0
34-35	37.37158336741962	39.0	36.0	41.0	31.0	41.0
36-37	37.46311038483124	39.0	36.0	41.0	32.0	41.0
38-39	37.43249161063724	39.0	36.0	41.0	31.0	41.0
40-41	37.400607015546385	39.0	36.0	41.0	31.0	41.0
42-43	37.48460453566052	39.5	36.0	41.0	31.5	41.0
44-45	37.524816513176155	40.0	36.0	41.0	32.0	41.0
46-47	37.41059489347444	39.5	36.0	41.0	31.0	41.0
48-49	37.351334731851104	39.0	36.0	41.0	31.0	41.0
50-51	37.26011915442116	39.0	36.0	41.0	31.0	41.0
52-53	37.112340064608375	39.0	35.5	41.0	31.0	41.0
54-55	36.9441702041537	39.0	35.0	41.0	31.0	41.0
56-57	36.8091339298552	39.0	35.0	40.0	31.0	41.0
58-59	36.553284192961016	38.0	35.0	40.0	30.0	41.0
60-61	36.398531593286506	38.0	35.0	40.0	30.0	41.0
62-63	36.1451918256942	38.0	34.5	40.0	29.5	41.0
64-65	35.844492930808514	37.0	34.0	40.0	29.0	41.0
66-67	35.517744216704784	37.0	34.0	39.5	29.0	41.0
68-69	35.28876387800946	36.0	34.0	39.0	29.0	41.0
70-71	34.96265401329727	36.0	34.0	39.0	29.0	40.5
72-73	34.667408427969406	35.5	33.0	38.5	28.5	40.0
74-75	34.383687090237615	35.0	33.0	37.5	28.0	40.0
76-77	33.01435504733506	34.0	31.5	36.0	26.5	39.0
78-79	33.762911191808456	35.0	32.5	36.5	28.5	39.0
80-81	33.784500451185444	35.0	33.0	36.0	29.0	39.0
82-83	33.54608940368004	35.0	33.0	36.0	29.0	37.5
84-85	33.33554142297221	35.0	33.0	35.5	29.0	37.0
86-87	33.157211771436366	35.0	33.0	35.0	29.0	37.0
88-89	33.035707492308035	35.0	32.0	35.0	29.0	36.0
90-91	32.896717727944676	35.0	32.0	35.0	28.0	36.0
92-93	32.88430073124099	34.0	32.0	35.0	29.0	36.0
94-95	32.819341881837744	34.0	32.0	35.0	29.0	35.0
96-97	32.916974564463786	34.0	32.0	35.0	29.0	35.0
98-99	33.038675397221056	34.0	32.0	35.0	29.0	35.0
100	31.9833968501751	34.0	31.0	35.0	26.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	5.0
19	30.0
20	150.0
21	784.0
22	3191.0
23	10592.0
24	27403.0
25	57089.0
26	101535.0
27	157818.0
28	223569.0
29	295421.0
30	369868.0
31	452426.0
32	551684.0
33	682209.0
34	880885.0
35	1172263.0
36	1699319.0
37	2619062.0
38	2937882.0
39	1512632.0
40	54596.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.9781447552445	20.07201811096915	13.436055324912394	24.513781808873954
2	15.671310384228136	21.29129356358951	39.389127698190094	23.648268353992258
3	18.628207230857626	25.4561654463902	30.322702572953197	25.592924749798975
4	13.122885879968123	17.383460236350608	37.78472261695249	31.70893126672877
5	13.43367306485721	37.61884056344433	35.44978192183218	13.49770444986628
6	31.252435209224345	37.15097627406562	18.065257271414364	13.53133124529567
7	27.316796779821605	32.184470922850615	22.659072880865637	17.83965941646214
8	25.243890208947377	36.08013951789283	20.850133757747326	17.825836515412462
9	25.77982631224333	16.104657245998762	20.963012335255673	37.152504106502235
10-11	24.753665983245256	26.349501445105016	28.614596302862733	20.282236268787
12-13	25.17595235190253	23.883159919524505	28.82847112125161	22.112416607321357
14-15	22.27009470750879	25.358821585013917	26.230410165081935	26.14067354239536
16-17	22.16802681164903	28.44506482969556	26.99557906116507	22.391329297490337
18-19	22.15337665753635	27.747039465526157	28.503555429159604	21.596028447777886
20-21	22.87541994256202	26.980934410354045	28.365726531680142	21.777919115403797
22-23	22.635572886878354	26.889768378762874	28.163623260084975	22.3110354742738
24-25	22.39179468813152	26.98405471656713	28.307592128694647	22.3165584666067
26-27	22.344082503007222	27.150086688021148	28.224742748835567	22.281088060136067
28-29	22.344371125122173	27.262558729502473	28.185072959676788	22.20799718569856
30-31	22.54864812791846	27.111944638590106	28.073417108101044	22.265990125390385
32-33	22.342055948391444	27.254975892546195	28.074055269734316	22.32891288932804
34-35	22.484596083558568	27.283574319804693	28.030298788236585	22.201530808400154
36-37	22.46536295142663	27.244034164796332	27.97941889145977	22.311183992317275
38-39	22.282966015354596	27.402266415645038	28.044516321036472	22.27025124796389
40-41	22.565748897233195	27.265175814678937	27.881484203205286	22.28759108488258
42-43	22.428281435839395	27.361720105735937	27.7115263068827	22.498472151541968
44-45	22.528684899719714	27.436322048525767	27.565876579106224	22.469116472648295
46-47	22.5813246566857	27.413450963677853	27.412814648594026	22.592409731042416
48-49	22.466985553966065	27.542308700722916	27.36892203187063	22.621783713440387
50-51	22.48856668909564	27.627681662275293	27.323445368880446	22.560306279748623
52-53	22.655910831104865	27.531426229632753	27.176607637572708	22.636055301689673
54-55	22.615530043135077	27.406989608585917	27.292093426335928	22.68538692194307
56-57	22.609883320537367	27.52682210747955	27.2449263710084	22.61836820097469
58-59	22.531429066187922	27.60327095540564	27.2354292998574	22.62987067854904
60-61	22.62346786334058	27.512238326207484	27.304304286195418	22.55998952425652
62-63	22.638001073575076	27.635210129357624	27.18707963823747	22.53970915882983
64-65	22.57472510651823	27.5807383844984	27.21075153208214	22.633784976901236
66-67	22.658815610341275	27.549399604118353	27.111035821737484	22.68074896380289
68-69	22.598330288056996	27.533715920468047	27.188265504453707	22.679688287021246
70-71	22.576784859391445	27.36123768998314	27.246442173936636	22.81553527668878
72-73	22.655578516342974	27.38649194348169	27.158453797884015	22.79947574229132
74-75	22.472886760317497	27.538774372337848	27.170038504794526	22.818300362550133
76-77	22.622435773813606	27.3922703386765	27.160863595121366	22.82443029238853
78-79	22.530600758121928	27.41000068604192	27.14394700511017	22.915451550725983
80-81	22.618243481382027	27.411296077602188	27.13609597520033	22.834364465815455
82-83	22.613822228981842	27.39027635462403	27.231291974219534	22.76460944217459
84-85	22.691115107322606	27.34775101207657	27.184539520406904	22.776594360193915
86-87	22.56529480650068	27.347077625610584	27.26629832508969	22.821329242799052
88-89	22.5670912712343	27.287525346676915	27.298502310569916	22.846881071518865
90-91	22.590082211719402	27.337560616963614	27.29618623442614	22.776170936890843
92-93	22.63452940031663	27.23747608362886	27.34785912542776	22.78013539062675
94-95	22.484481340464537	27.28344394431279	27.44063855093417	22.791436164288502
96-97	22.478933159270735	27.435334860994026	27.493417873630094	22.592314106105146
98-99	23.61128675730604	29.415991315882483	27.669441183857334	19.303280742954144
100	30.40822666072996	40.88499423505542	28.70677910421462	0.0
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2109.0
1	1993.0
2	3001.5
3	4338.5
4	5867.0
5	9706.5
6	13195.5
7	15775.0
8	17613.0
9	17561.0
10	15929.5
11	14209.0
12	15148.5
13	19775.0
14	30596.5
15	45218.5
16	57289.0
17	64621.0
18	67606.0
19	67305.5
20	64664.5
21	61967.0
22	61915.0
23	66562.5
24	77524.0
25	95105.5
26	118819.5
27	145599.5
28	173458.5
29	204737.5
30	235534.5
31	264411.0
32	293387.5
33	321234.5
34	348656.0
35	376747.5
36	408013.0
37	437325.5
38	461280.0
39	481961.0
40	502501.5
41	524071.0
42	541136.5
43	555074.5
44	568193.0
45	580414.5
46	590477.0
47	597790.0
48	602461.0
49	603483.0
50	601282.0
51	594103.5
52	577307.5
53	553671.5
54	528923.5
55	498809.5
56	463613.5
57	425073.0
58	381999.0
59	334436.5
60	286360.5
61	239302.0
62	193951.0
63	153389.5
64	118785.5
65	92742.0
66	72737.5
67	55825.5
68	42111.0
69	31541.5
70	23117.0
71	16589.5
72	11900.0
73	8342.0
74	5692.5
75	3858.5
76	2576.5
77	1688.5
78	1155.5
79	802.5
80	539.5
81	369.5
82	267.0
83	170.0
84	104.0
85	72.5
86	53.5
87	42.5
88	31.5
89	23.0
90	15.0
91	9.0
92	7.0
93	4.0
94	1.5
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	1.4481824043665594E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0014300801243119776
20-21	0.0032259759201716145
22-23	0.005131006323594138
24-25	0.0012387092736295747
26-27	0.0018190655242983488
28-29	0.004442482096343837
30-31	0.008402954090661954
32-33	0.014642562050407659
34-35	1.8665057519119606E-4
36-37	8.323178564441731E-4
38-39	0.0017866473406645802
40-41	0.0027406729855613023
42-43	2.72643383249919E-4
44-45	1.1490082985975357E-5
46-47	0.0041979513299144735
48-49	0.003469263231428146
50-51	7.752347868124214E-4
52-53	0.0012864320056564416
54-55	0.0010167700355755635
56-57	0.0015116039077143985
58-59	8.094912768761013E-4
60-61	4.860816027877245E-4
62-63	0.0018019574308315858
64-65	5.548753275335857E-4
66-67	0.002118567614459022
68-69	0.0017703658066286064
70-71	5.249422461708517E-4
72-73	8.493909155483669E-4
74-75	0.00888188729346119
76-77	0.0036214352088128525
78-79	0.006562335611572715
80-81	8.780639003198446E-5
82-83	0.004924008613097684
84-85	0.0029132359700974977
86-87	8.204521070568983E-4
88-89	0.00406982715825442
90-91	9.332881372998044E-4
92-93	5.449934908702418E-5
94-95	6.423360567078847E-4
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	401.0
20-21	1281.0
22-23	2653.0
24-25	5882.0
26-27	9116.0
28-29	18659.0
30-31	40122.0
32-33	53518.0
34-35	76530.0
36-37	110004.0
38-39	125009.0
40-41	129348.0
42-43	142868.0
44-45	165855.0
46-47	164009.0
48-49	152780.0
50-51	140610.0
52-53	140394.0
54-55	152525.0
56-57	157025.0
58-59	154535.0
60-61	149487.0
62-63	145940.0
64-65	155701.0
66-67	162988.0
68-69	163737.0
70-71	160662.0
72-73	154613.0
74-75	159196.0
76-77	157935.0
78-79	165534.0
80-81	167762.0
82-83	162026.0
84-85	167605.0
86-87	176864.0
88-89	191725.0
90-91	199491.0
92-93	215647.0
94-95	247795.0
96-97	523728.0
98-99	2756582.0
100-101	5482273.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.16125469064427
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.6330731713708	75.43631899880893
2	7.030221197512626	11.833444734708525
3	1.7689163725778432	4.466226640769233
4	0.70139792987718	2.3612211926353597
5	0.34115323753718124	1.4355942256452288
6	0.1625862980681856	0.8210080104555343
7	0.10537648517682194	0.6208032045159988
8	0.06531808435847422	0.43978015468788295
9	0.04379346480182101	0.33171416504746404
>10	0.143379086948223	1.8952359504395746
>50	0.003754291398219604	0.2043871441135864
>100	9.83999549178652E-4	0.12424884299802842
>500	3.092315592854311E-5	0.016369909191474935
>1k	1.545766760249417E-5	0.013646825983176571
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	7.240912021832798E-6	0.0	0.0	0.0
3	0.0	1.4481824043665595E-5	0.0	0.0	0.0
4	0.0	5.0686384152829585E-5	0.0	1.4481824043665595E-5	7.240912021832798E-6
5	0.0	5.0686384152829585E-5	0.0	1.4481824043665595E-5	7.240912021832798E-6
6	0.0	5.0686384152829585E-5	0.0	1.4481824043665595E-5	3.620456010916399E-5
7	0.0	5.0686384152829585E-5	0.0	1.4481824043665595E-5	3.620456010916399E-5
8	0.0	5.792729617466238E-5	0.0	2.896364808733119E-5	5.0686384152829585E-5
9	0.0	1.9550462458948554E-4	0.0	3.620456010916399E-5	7.240912021832799E-5
10-11	1.4481824043665595E-5	1.9550462458948554E-4	0.0	5.0686384152829585E-5	7.240912021832799E-5
12-13	2.896364808733119E-5	1.9550462458948554E-4	0.0	6.154775218557878E-5	7.240912021832799E-5
14-15	3.982501612008039E-5	3.258410409824759E-4	0.0	7.602957622924439E-5	9.051140027290997E-5
16-17	5.792729617466238E-5	3.548046890698071E-4	0.0	7.965003224016078E-5	1.0861368032749197E-4
18-19	6.516820819649518E-5	4.199728972663023E-4	0.0	9.413185628382637E-5	1.1585459234932476E-4
20-21	6.516820819649518E-5	4.706592814191319E-4	0.0	1.2309550437115756E-4	1.1585459234932476E-4
22-23	7.240912021832799E-5	4.99622929506463E-4	0.0	1.3757732841482317E-4	1.4119778442573956E-4
24-25	8.327048825107718E-5	5.503093136592927E-4	0.0	1.7378188852398716E-4	1.6654097650215435E-4
26-27	9.413185628382637E-5	5.828934177575403E-4	0.0	2.534319207641479E-4	1.7740234453490354E-4
28-29	9.413185628382637E-5	5.90134329779373E-4	0.0	7.059889221286978E-4	1.8102280054581996E-4
30-31	1.0861368032749196E-4	6.553025379758682E-4	0.0	0.002143309958462508	1.8464325655673635E-4
32-33	1.1585459234932476E-4	6.62543449997701E-4	0.0	0.004460401805449004	2.1722736065498394E-4
34-35	1.3395687240390675E-4	7.639162183033602E-4	0.0	0.0077948417915030075	2.2446827267681675E-4
36-37	1.5205915245848875E-4	8.689094426199358E-4	0.0	0.013316037208150515	2.3170918469864952E-4
38-39	1.5205915245848875E-4	8.97873090707267E-4	0.0	0.021248456328068346	2.3170918469864952E-4
40-41	1.5930006448032155E-4	0.0010064867710347588	0.0	0.029412584632684827	2.4619100874231513E-4
42-43	1.5930006448032155E-4	0.0010680345232203378	0.0	0.037283456000417076	2.498114647532315E-4
44-45	1.6292052049123794E-4	0.0012237141316897428	0.0	0.04653734156431939	2.606728327859807E-4
46-47	1.6654097650215435E-4	0.0013323278120172348	0.0	0.05657686608259056	2.7153420081872995E-4
48-49	1.6654097650215435E-4	0.0013395687240390676	0.0	0.06614211086343169	3.041183049169775E-4
50-51	1.6654097650215435E-4	0.001524211980595804	0.0	0.07569649427624006	3.041183049169775E-4
52-53	1.6654097650215435E-4	0.0015350733486285532	0.0	0.08525811860107027	3.403228650261415E-4
54-55	1.7378188852398716E-4	0.0015386938046394695	0.0	0.09426219270021936	3.837683371571383E-4
56-57	1.7378188852398716E-4	0.0015567960846940515	0.0	0.10329160999144486	3.910092491789711E-4
58-59	1.7378188852398716E-4	0.0015640369967158843	0.0	0.11146659966409408	3.910092491789711E-4
60-61	1.7378188852398716E-4	0.0016111029248577977	0.0	0.1191311050392041	3.982501612008039E-4
62-63	1.7378188852398716E-4	0.0016292052049123795	0.0	0.1267919899583032	4.127319852444695E-4
64-65	1.7378188852398716E-4	0.0017812643573708682	0.0	0.13418496113259448	4.127319852444695E-4
66-67	1.7378188852398716E-4	0.001795746181414534	0.0	0.14212100070852324	4.127319852444695E-4
68-69	1.7378188852398716E-4	0.0018319507415236979	0.0	0.14981084927570967	4.127319852444695E-4
70-71	1.7378188852398716E-4	0.001868155301632862	0.0	0.15633491100738103	4.2721380928813506E-4
72-73	1.7378188852398716E-4	0.001879016669665611	0.0	0.16175111319971197	4.851411054627975E-4
74-75	1.7378188852398716E-4	0.0019297030538184407	0.0	0.16628030366936836	4.887615614737139E-4
76-77	1.8826371256765273E-4	0.0019803894379712703	0.0	0.1707407054748174	4.923820174846303E-4
78-79	1.8826371256765273E-4	0.0019912508060040196	0.0	0.1734162224668846	4.923820174846303E-4
80-81	1.8826371256765273E-4	0.001994871262014936	0.0	0.17438288422179926	4.960024734955466E-4
82-83	1.8826371256765273E-4	0.0020057326300476847	0.0	0.1749838799196114	5.141047535501287E-4
84-85	1.8826371256765273E-4	0.002016593998080434	0.0	0.1751793845442009	5.141047535501287E-4
86-87	1.8826371256765273E-4	0.0020491781021786818	0.0	0.17535316643272486	5.141047535501287E-4
88	1.8826371256765273E-4	0.0021071053983533444	0.0	0.17536764825676854	5.141047535501287E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	14765	0.0	45.459377	1
GTATCAA	25105	0.0	32.48483	1
TCAACGC	29510	0.0	26.7954	4
ATCAACG	30160	0.0	26.231392	3
CAACGCA	30705	0.0	25.805521	5
TATCAAC	31620	0.0	25.17449	2
AACGCAG	31555	0.0	25.09751	6
GTACATG	38255	0.0	21.955936	1
TACATGG	38745	0.0	21.436928	2
ACGCAGA	37735	0.0	20.976423	7
CGCAGAG	38495	0.0	20.593973	8
ACATGGG	40070	0.0	20.312088	3
CATGGGG	29795	0.0	18.638765	4
GTGGTAT	8100	0.0	18.319674	1
GAGTACT	25185	0.0	17.337275	12-13
GCAGAGT	46195	0.0	17.073267	9
TGGTATC	8220	0.0	16.420113	2
CAGAGTA	44050	0.0	16.38644	10-11
AGAGTAC	40085	0.0	16.15637	10-11
ATGGGGG	18980	0.0	15.379354	5
>>END_MODULE