FastQCFastQC Report
Fri 27 May 2016
SRR2138717_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138717_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6427710
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA67350.044.8759351
GTATCAA114400.032.6066061
TCAACGC132750.027.1188354
ATCAACG136150.026.5013643
CAACGCA137200.026.1799555
AACGCAG139850.025.742056
TATCAAC143000.025.4025732
GTACATG173900.021.7543261
TACATGG172400.021.6368662
ACGCAGA169400.021.2756397
CGCAGAG172050.020.9715828
ACATGGG178300.020.5102353
CATGGGG141500.018.1399124
GCAGAGT206200.017.3405349
GAGTACT114950.017.02198412-13
GTGGTAT38300.016.8873181
CAGAGTA197650.016.46476210-11
AGAGTAC179450.016.2418310-11
GTACTTT128250.015.29006814-15
TGGTATC38800.015.0970832
Produced by FastQC (version 0.11.5)