##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138717_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6427710
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.781684612404728	31.0	31.0	34.0	30.0	34.0
2	32.051940737836645	33.0	31.0	34.0	30.0	34.0
3	31.963203224787677	31.0	31.0	34.0	30.0	34.0
4	35.810650760535246	37.0	35.0	37.0	35.0	37.0
5	35.593603631775544	37.0	35.0	37.0	33.0	37.0
6	35.6751446471605	37.0	35.0	37.0	33.0	37.0
7	35.592260385113825	37.0	35.0	37.0	33.0	37.0
8	35.577218325033336	37.0	35.0	37.0	33.0	37.0
9	37.30456072224789	39.0	37.0	39.0	34.0	39.0
10-11	37.180113368524715	39.0	37.0	39.0	33.0	39.0
12-13	37.144547124870286	39.0	37.0	39.0	33.0	39.0
14-15	38.34329146772334	40.0	38.0	41.0	33.0	41.0
16-17	38.29703510270376	40.0	38.0	41.0	33.0	41.0
18-19	38.29141537188205	40.0	38.0	41.0	33.5	41.0
20-21	38.288585256111205	40.0	38.0	41.0	33.5	41.0
22-23	38.222444381208874	40.0	38.0	41.0	33.0	41.0
24-25	38.143546349098784	40.0	37.0	41.0	33.0	41.0
26-27	37.97727398390056	40.0	37.0	41.0	33.0	41.0
28-29	37.83146069809403	40.0	37.0	41.0	33.0	41.0
30-31	37.67997223591916	40.0	37.0	41.0	32.0	41.0
32-33	37.52255363726784	39.5	36.5	41.0	32.0	41.0
34-35	37.395631959364124	39.0	36.0	41.0	31.5	41.0
36-37	37.48758659595771	39.0	36.0	41.0	32.0	41.0
38-39	37.45660360971755	39.0	36.0	41.0	31.0	41.0
40-41	37.423940041866615	39.0	36.0	41.0	31.0	41.0
42-43	37.50877130105968	39.5	36.5	41.0	32.0	41.0
44-45	37.54908255687821	40.0	36.5	41.0	32.0	41.0
46-47	37.43684545182981	40.0	36.0	41.0	31.0	41.0
48-49	37.37614773655129	39.0	36.0	41.0	31.0	41.0
50-51	37.281019587806256	39.0	36.0	41.0	31.0	41.0
52-53	37.129163629542234	39.0	35.5	41.0	31.0	41.0
54-55	36.95926236116466	39.0	35.0	41.0	31.0	41.0
56-57	36.821627337838414	39.0	35.0	40.0	31.0	41.0
58-59	36.566299334499504	38.0	35.0	40.0	30.0	41.0
60-61	36.410246649296326	38.0	35.0	40.0	30.0	41.0
62-63	36.15460613593302	38.0	34.5	40.0	29.5	41.0
64-65	35.851893111911316	37.0	34.0	40.0	29.0	41.0
66-67	35.52107588489832	37.0	34.0	39.5	29.0	41.0
68-69	35.29424229231361	36.0	34.0	39.0	29.0	41.0
70-71	34.96620319261531	36.0	34.0	39.0	29.0	40.5
72-73	34.670510045501246	35.0	33.0	38.5	29.0	40.0
74-75	34.386559529443545	35.0	33.0	37.5	28.5	40.0
76-77	33.01932267941172	34.0	31.5	36.0	26.5	39.0
78-79	33.764497608961626	35.0	32.5	36.0	28.5	39.0
80-81	33.784916619026035	35.0	33.0	36.0	29.0	39.0
82-83	33.551825510590106	35.0	33.0	36.0	29.0	37.5
84-85	33.34551964289634	35.0	33.0	35.5	29.0	37.0
86-87	33.172564308210056	35.0	33.0	35.0	29.0	37.0
88-89	33.04871470939854	35.0	32.5	35.0	29.0	36.0
90-91	32.90877916197151	35.0	32.0	35.0	28.5	36.0
92-93	32.89776878256822	34.0	32.0	35.0	29.0	36.0
94-95	32.834606807919045	34.0	32.0	35.0	29.0	35.0
96-97	32.933640448038815	34.0	32.0	35.0	29.0	35.0
98-99	33.05495485649824	34.0	32.0	35.0	29.0	35.0
100	32.00114009246033	34.0	31.0	35.0	26.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	0.0
15	0.0
16	0.0
17	3.0
18	2.0
19	14.0
20	55.0
21	332.0
22	1401.0
23	4929.0
24	12538.0
25	26552.0
26	47111.0
27	73527.0
28	104051.0
29	136586.0
30	171039.0
31	209318.0
32	253879.0
33	315773.0
34	407204.0
35	542407.0
36	789549.0
37	1222176.0
38	1369739.0
39	713554.0
40	25970.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.94496951480387	20.05026673574259	13.470831758122257	24.533931991331283
2	15.626856220955831	21.201905499781414	39.502902277794114	23.66833600146864
3	18.715337501960267	25.374333198085285	30.239995793204905	25.670333506749543
4	13.140169671624887	17.26677152516215	37.75076971425282	31.84228908896014
5	13.51017703038874	37.57023885645121	35.396945412907556	13.522638700252502
6	31.421657168727275	37.03709408171807	18.013662719693325	13.527586029861336
7	27.366869382719507	32.09161583207705	22.652126496061584	17.889388289141856
8	25.19508503028295	36.208198565274415	20.782533748411176	17.814182656031463
9	25.689662414763582	16.228796881004275	20.957370509870547	37.1241701943616
10-11	24.83209416728508	26.359675529854332	28.524186685460297	20.28404361740029
12-13	25.185874907237572	23.817378195344844	28.818093846797694	22.178653050619896
14-15	22.311141915238863	25.34075899503867	26.137753570089505	26.210345519632966
16-17	22.221382109647138	28.426858087872663	26.913667542561814	22.438092259918385
18-19	22.232541416625644	27.692897455540255	28.425354548469944	21.649206579364154
20-21	22.907771718739074	26.951230317988934	28.26157901382098	21.87941894945101
22-23	22.670924861054583	26.846441477823856	28.081815281120893	22.40081838000067
24-25	22.44856809966167	26.97017228375349	28.189966907897745	22.39129270868709
26-27	22.449087792950824	27.11041450849005	28.08788653128071	22.352611167278415
28-29	22.40911009761754	27.219091480425927	28.091241536205818	22.28055688575072
30-31	22.599994895362343	27.08566791803138	27.972930434325832	22.34140675228044
32-33	22.402079565571793	27.225743432086496	27.95866643418408	22.413510568157626
34-35	22.57298443774311	27.252227110212473	27.896650451396642	22.278138000647772
36-37	22.565315009409847	27.188563927678764	27.85496991941569	22.391151143495694
38-39	22.37477551716027	27.326674673101603	27.975923567163573	22.322626242574554
40-41	22.66530756451165	27.20126671284521	27.79641751431362	22.337008208329518
42-43	22.500539445043014	27.286761014174576	27.64992363182463	22.56277590895778
44-45	22.587139802217873	27.39098055195602	27.52535118887909	22.496528456947026
46-47	22.683355848226018	27.356702719933452	27.32118490045263	22.638756531387905
48-49	22.52746636208529	27.427147626491728	27.348254682386084	22.6971313290369
50-51	22.557289135786387	27.542256747205013	27.312769878171206	22.587684238837397
52-53	22.735048636109042	27.49915207209415	27.085613533015678	22.680185758781125
54-55	22.691603343179807	27.314402661777592	27.262583409703296	22.731410585339308
56-57	22.62495664204913	27.470466208024114	27.231506883704515	22.67307026622224
58-59	22.626019818476877	27.557860002772166	27.172774325305394	22.643345853445563
60-61	22.6832950989556	27.46738710670393	27.24644276987524	22.602875024465224
62-63	22.727851282914997	27.57523840625002	27.123248698295722	22.57366161253927
64-65	22.64608642025714	27.558627596265993	27.112853908604073	22.682432074872793
66-67	22.73197964632717	27.52575063573407	27.023206992334607	22.719062725604154
68-69	22.616742268104343	27.492975220958098	27.194337825806137	22.695944685131415
70-71	22.667837410024333	27.277023217144915	27.170606410838737	22.884532961992015
72-73	22.738611740889773	27.327441603990387	27.099451502788654	22.83449515233119
74-75	22.520312783924695	27.457202876238284	27.177306791428794	22.845177548408223
76-77	22.713764728993212	27.351707594171486	27.103262289572967	22.831265387262334
78-79	22.617843400366443	27.359603576890894	27.072697863952104	22.94985515879056
80-81	22.692863706746692	27.36721711064909	27.08135923565375	22.85855994695046
82-83	22.683802975550833	27.339054354156755	27.201475099312074	22.775667570980342
84-85	22.7592826148705	27.29565431365194	27.15447422851238	22.790588842965175
86-87	22.588295362590028	27.309752796681718	27.277355961895267	22.824595878832984
88-89	22.65934454407195	27.26846735077587	27.232323020150993	22.839865085001186
90-91	22.64848644937064	27.272959425349296	27.251036548502398	22.827517576777666
92-93	22.71385664621222	27.185940129140988	27.314994941801313	22.78520828284548
94-95	22.564036664583398	27.25621275977329	27.370983472492377	22.80876710315094
96-97	22.562059515620014	27.36839137219161	27.460528400872786	22.60902071131559
98-99	23.685557284985688	29.398040181347113	27.593843083883968	19.322559449783228
100	30.560470231718927	40.85947407106784	28.58005569721323	0.0
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1048.0
1	963.5
2	1350.5
3	1912.0
4	2529.0
5	4164.0
6	5651.5
7	6734.0
8	7489.0
9	7512.0
10	6925.0
11	6207.5
12	6594.0
13	8560.5
14	13287.5
15	19620.5
16	24832.5
17	28173.0
18	29572.5
19	29510.0
20	28767.5
21	27887.5
22	27983.0
23	30465.5
24	35794.5
25	44273.5
26	55705.5
27	68112.5
28	80475.5
29	93934.5
30	107279.5
31	120220.5
32	133670.5
33	147314.0
34	161024.0
35	174134.0
36	188587.0
37	202306.0
38	213019.5
39	222010.5
40	232086.0
41	242091.0
42	249503.0
43	257243.0
44	264906.0
45	271469.5
46	276739.5
47	280337.5
48	281802.0
49	281492.5
50	280671.0
51	276959.5
52	267811.0
53	257675.0
54	247250.5
55	233436.5
56	217675.5
57	200679.5
58	181541.5
59	158947.0
60	135388.0
61	112548.0
62	91504.0
63	72578.5
64	56115.5
65	44070.0
66	34366.0
67	26359.0
68	20182.0
69	15204.5
70	11053.0
71	7767.5
72	5446.0
73	3748.0
74	2527.0
75	1706.0
76	1167.0
77	759.5
78	487.5
79	338.5
80	245.0
81	186.0
82	148.0
83	106.0
84	68.0
85	42.0
86	28.0
87	23.0
88	16.5
89	10.5
90	6.5
91	3.5
92	1.5
93	1.5
94	2.0
95	2.0
96	2.0
97	2.0
98	1.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	2.1780696391094184E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0015246487473765929
20-21	0.002886078769568936
22-23	0.005453805643794137
24-25	0.0010894656762282324
26-27	0.0016115509745640423
28-29	0.004222855841051083
30-31	0.008975239188173225
32-33	0.014685898304538092
34-35	1.8080989152742928E-4
36-37	8.543187911135974E-4
38-39	0.0021542228912192437
40-41	0.002609253427363096
42-43	3.6599982041608814E-4
44-45	4.112713689406053E-5
46-47	0.0037407704404153175
48-49	0.003619275807376911
50-51	7.852646790081253E-4
52-53	0.001061516797036176
54-55	0.0010650069784145782
56-57	0.0013170743306367488
58-59	5.820081232216854E-4
60-61	4.081299855911976E-4
62-63	0.0017540030067835837
64-65	5.115150405882535E-4
66-67	0.0022156270912926986
68-69	0.0019989100679839835
70-71	5.045724452017344E-4
72-73	9.744754369193505E-4
74-75	0.008675486778168802
76-77	0.003950978287550781
78-79	0.006581388327414197
80-81	1.1490710804924292E-4
82-83	0.004755966880635635
84-85	0.002686092835251894
86-87	7.240689515503523E-4
88-89	0.0035752000883074423
90-91	0.001014621031064732
92-93	4.658175511435646E-5
94-95	8.352097712861562E-4
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	194.0
20-21	595.0
22-23	1181.0
24-25	2555.0
26-27	4008.0
28-29	8394.0
30-31	18133.0
32-33	24703.0
34-35	34982.0
36-37	51592.0
38-39	58352.0
40-41	59756.0
42-43	66021.0
44-45	76362.0
46-47	76159.0
48-49	70340.0
50-51	65135.0
52-53	64243.0
54-55	70257.0
56-57	72739.0
58-59	71578.0
60-61	68863.0
62-63	67994.0
64-65	71462.0
66-67	75511.0
68-69	74956.0
70-71	74261.0
72-73	71099.0
74-75	73098.0
76-77	72162.0
78-79	75507.0
80-81	76867.0
82-83	74715.0
84-85	76540.0
86-87	80648.0
88-89	87899.0
90-91	91465.0
92-93	99536.0
94-95	114843.0
96-97	244078.0
98-99	1288960.0
100-101	2569967.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.52672512465952
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.25051689307239	79.86858909583047
2	6.310222470846681	11.046262153624946
3	1.408062581127424	3.6972931948997596
4	0.49510669196322726	1.7334026933937947
5	0.21649626680445921	0.9474604617554427
6	0.10637173881639989	0.5586221966649064
7	0.06356632653322651	0.3894626672760648
8	0.03965655729900386	0.2776806872080177
9	0.02432074182572259	0.1915843396147048
>10	0.08366683586481737	1.1286087194465906
>50	0.0016293186298497351	0.09265595251030896
>100	3.572932507498911E-4	0.053332244205920525
>500	2.6283966093011277E-5	0.015045593569174004
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.6672920838058965E-5
2	0.0	3.1115280558705977E-5	0.0	3.1115280558705977E-5	4.6672920838058965E-5
3	0.0	3.1115280558705977E-5	0.0	3.1115280558705977E-5	4.6672920838058965E-5
4	0.0	1.0890348195547092E-4	0.0	3.1115280558705977E-5	4.6672920838058965E-5
5	0.0	1.244611222348239E-4	0.0	3.1115280558705977E-5	4.6672920838058965E-5
6	0.0	1.244611222348239E-4	0.0	3.1115280558705977E-5	4.6672920838058965E-5
7	0.0	1.244611222348239E-4	0.0	3.1115280558705977E-5	4.6672920838058965E-5
8	0.0	1.400187625141769E-4	0.0	3.1115280558705977E-5	4.6672920838058965E-5
9	0.0	3.578257264251187E-4	0.0	3.1115280558705977E-5	6.223056111741195E-5
10-11	1.5557640279352988E-5	3.578257264251187E-4	0.0	3.1115280558705977E-5	6.223056111741195E-5
12-13	1.5557640279352988E-5	3.578257264251187E-4	0.0	3.1115280558705977E-5	6.223056111741195E-5
14-15	2.3336460419029482E-5	5.600750500567076E-4	0.0	3.1115280558705977E-5	1.0112466181579443E-4
16-17	3.1115280558705977E-5	5.9896915075509E-4	0.0	3.1115280558705977E-5	1.0890348195547092E-4
18-19	3.1115280558705977E-5	6.61199711872502E-4	0.0	3.1115280558705977E-5	1.322399423745004E-4
20-21	3.1115280558705977E-5	7.467667334089434E-4	0.0	3.1115280558705977E-5	1.5557640279352988E-4
22-23	4.6672920838058965E-5	8.089972945263554E-4	0.0	7.000938125708845E-5	1.8669168335223586E-4
24-25	9.334584167611793E-5	8.556702153644145E-4	0.0	9.334584167611793E-5	2.1780696391094184E-4
26-27	1.0890348195547092E-4	8.867854959231203E-4	0.0	1.7891286321255936E-4	2.1780696391094184E-4
28-29	1.0890348195547092E-4	8.867854959231203E-4	0.0	7.623243736882964E-4	2.1780696391094184E-4
30-31	1.400187625141769E-4	9.645736973198854E-4	0.0	0.002030272056455565	2.1780696391094184E-4
32-33	1.5557640279352988E-4	9.645736973198854E-4	0.0	0.004776195565761367	2.3336460419029484E-4
34-35	1.5557640279352988E-4	0.0011434865605324447	0.0	0.008307779909174497	2.489222444696478E-4
36-37	1.5557640279352988E-4	0.0013301782438846807	0.0	0.013986318611138337	2.489222444696478E-4
38-39	1.5557640279352988E-4	0.0014468605459798279	0.0	0.022480790203665068	2.567010646093243E-4
40-41	1.7113404307288288E-4	0.0014935334668178869	0.0	0.031231962860801127	2.800375250283538E-4
42-43	1.7113404307288288E-4	0.0015402063876559459	0.0	0.039687540352629475	2.800375250283538E-4
44-45	1.7113404307288288E-4	0.001711340430728829	0.0	0.04908435508135868	2.800375250283538E-4
46-47	1.7113404307288288E-4	0.0018202439126842997	0.0	0.05936795530601101	2.800375250283538E-4
48-49	1.7113404307288288E-4	0.0018202439126842997	0.0	0.0694648638473111	3.2671044586641277E-4
50-51	1.7113404307288288E-4	0.0019524838550588	0.0	0.07864387161212935	3.2671044586641277E-4
52-53	1.7113404307288288E-4	0.0019602626751984765	0.0	0.08821959920407113	3.422680861457657E-4
54-55	1.7113404307288288E-4	0.001983599135617506	0.0	0.09800535493978416	3.578257264251187E-4
56-57	1.7113404307288288E-4	0.0020536085168745945	0.0	0.10680320051775827	3.578257264251187E-4
58-59	1.7113404307288288E-4	0.0020536085168745945	0.0	0.11555437317489432	3.578257264251187E-4
60-61	1.7113404307288288E-4	0.00210806025785233	0.0	0.124002171846583	3.733833667044717E-4
62-63	1.7113404307288288E-4	0.0021313967182713593	0.0	0.13218549063352267	3.889410069838247E-4
64-65	1.7113404307288288E-4	0.0022714154807855365	0.0	0.1399798684134785	3.889410069838247E-4
66-67	1.7113404307288288E-4	0.0022791943009252127	0.0	0.1472919593447744	3.889410069838247E-4
68-69	1.7113404307288288E-4	0.0023180884016235953	0.0	0.15453404089481323	3.889410069838247E-4
70-71	1.7891286321255936E-4	0.0023492036821823013	0.0	0.16147274845940468	3.889410069838247E-4
72-73	1.8669168335223586E-4	0.0023803189627410073	0.0	0.16692570137731788	4.200562875425307E-4
74-75	1.8669168335223586E-4	0.0024114342432997133	0.0	0.17125072537497804	4.200562875425307E-4
76-77	1.8669168335223586E-4	0.002442549523858419	0.0	0.17556797055249848	4.200562875425307E-4
78-79	1.8669168335223586E-4	0.0024658859842774487	0.0	0.17822832704026784	4.200562875425307E-4
80-81	1.8669168335223586E-4	0.0024736648044171253	0.0	0.1790684396153529	4.4339274796156023E-4
82-83	1.8669168335223586E-4	0.0024736648044171253	0.0	0.17962073584526994	4.667292083805897E-4
84-85	1.8669168335223586E-4	0.0024970012648361547	0.0	0.1798618792695999	4.667292083805897E-4
86-87	1.8669168335223586E-4	0.002543674185674214	0.0	0.18001745567239344	4.667292083805897E-4
88	1.8669168335223586E-4	0.002644798847490008	0.0	0.18004857095295215	4.667292083805897E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6735	0.0	44.875935	1
GTATCAA	11440	0.0	32.606606	1
TCAACGC	13275	0.0	27.118835	4
ATCAACG	13615	0.0	26.501364	3
CAACGCA	13720	0.0	26.179955	5
AACGCAG	13985	0.0	25.74205	6
TATCAAC	14300	0.0	25.402573	2
GTACATG	17390	0.0	21.754326	1
TACATGG	17240	0.0	21.636866	2
ACGCAGA	16940	0.0	21.275639	7
CGCAGAG	17205	0.0	20.971582	8
ACATGGG	17830	0.0	20.510235	3
CATGGGG	14150	0.0	18.139912	4
GCAGAGT	20620	0.0	17.340534	9
GAGTACT	11495	0.0	17.021984	12-13
GTGGTAT	3830	0.0	16.887318	1
CAGAGTA	19765	0.0	16.464762	10-11
AGAGTAC	17945	0.0	16.24183	10-11
GTACTTT	12825	0.0	15.290068	14-15
TGGTATC	3880	0.0	15.097083	2
>>END_MODULE