FastQCFastQC Report
Fri 27 May 2016
SRR2138716_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138716_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10435464
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA118050.045.4525761
GTATCAA202550.032.5112271
TCAACGC236050.027.0878964
ATCAACG239550.026.726063
CAACGCA240800.026.5198025
TATCAAC250150.025.8048042
AACGCAG249150.025.7125956
ACGCAGA296750.021.5607857
GTACATG301950.021.5125711
CGCAGAG306350.020.9116788
TACATGG305450.020.7603662
ACATGGG303050.020.321183
GTGGTAT66500.018.1545451
CATGGGG222000.018.1272854
GAGTACT203750.017.75642212-13
GCAGAGT357700.017.5233049
TGGTATC66300.017.350962
CAGAGTA349050.016.41451310-11
GAGTACA217850.016.065571
AGAGTAC315950.016.01775210-11
Produced by FastQC (version 0.11.5)