##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138716_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10435464
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.69469455311235	31.0	31.0	34.0	30.0	34.0
2	31.972226726094785	31.0	31.0	34.0	30.0	34.0
3	31.87638958842654	31.0	31.0	34.0	30.0	34.0
4	35.74525540982174	37.0	35.0	37.0	35.0	37.0
5	35.516802798610584	37.0	35.0	37.0	33.0	37.0
6	35.60391516850616	37.0	35.0	37.0	33.0	37.0
7	35.518374554308274	37.0	35.0	37.0	33.0	37.0
8	35.50241925035628	37.0	35.0	37.0	33.0	37.0
9	37.216237533855704	39.0	37.0	39.0	33.0	39.0
10-11	37.086513642325826	39.0	37.0	39.0	33.0	39.0
12-13	37.043812761943315	39.0	37.0	39.0	33.0	39.0
14-15	38.21192124279285	40.0	37.5	41.0	33.0	41.0
16-17	38.1609562833047	40.0	37.0	41.0	33.0	41.0
18-19	38.16028760196959	40.0	37.5	41.0	33.0	41.0
20-21	38.16044246639339	40.0	37.5	41.0	33.0	41.0
22-23	38.09178508565725	40.0	37.0	41.0	33.0	41.0
24-25	38.00739553014013	40.0	37.0	41.0	33.0	41.0
26-27	37.832201136235085	40.0	37.0	41.0	32.5	41.0
28-29	37.67782998583359	40.0	37.0	41.0	32.0	41.0
30-31	37.519976855569254	39.0	36.5	41.0	32.0	41.0
32-33	37.354974717737534	39.0	36.0	41.0	31.5	41.0
34-35	37.22375255790142	39.0	36.0	40.5	31.0	41.0
36-37	37.316806330221524	39.0	36.0	41.0	31.0	41.0
38-39	37.28696584211414	39.0	36.0	41.0	31.0	41.0
40-41	37.2545452423076	39.0	36.0	41.0	31.0	41.0
42-43	37.33964091229977	39.0	36.0	41.0	31.0	41.0
44-45	37.38133086632909	39.0	36.0	41.0	31.0	41.0
46-47	37.2661665922563	39.0	36.0	41.0	31.0	41.0
48-49	37.20741597435221	39.0	36.0	41.0	31.0	41.0
50-51	37.11674274652159	39.0	36.0	41.0	31.0	41.0
52-53	36.96774540945481	39.0	35.0	41.0	31.0	41.0
54-55	36.79677668746732	39.0	35.0	40.0	30.0	41.0
56-57	36.66381481999533	39.0	35.0	40.0	30.0	41.0
58-59	36.40713065568981	38.0	35.0	40.0	30.0	41.0
60-61	36.25571733232185	38.0	34.5	40.0	30.0	41.0
62-63	36.00364412236164	38.0	34.0	40.0	29.0	41.0
64-65	35.701192665565486	37.0	34.0	40.0	29.0	41.0
66-67	35.37283066516166	37.0	34.0	39.5	28.0	41.0
68-69	35.14612003133918	36.0	34.0	39.0	28.0	41.0
70-71	34.82401929488705	36.0	33.0	39.0	28.0	40.0
72-73	34.53013130278411	35.0	33.0	38.5	28.0	40.0
74-75	34.2467861810668	35.0	33.0	37.5	28.0	40.0
76-77	32.89268427401731	34.0	31.5	36.0	26.5	39.0
78-79	33.65389190722391	35.0	32.5	36.0	28.0	39.0
80-81	33.685821839041125	35.0	33.0	36.0	28.5	39.0
82-83	33.44669973998955	35.0	33.0	36.0	28.0	37.5
84-85	33.232153853662055	35.0	32.0	35.5	28.0	37.0
86-87	33.054873771950376	35.0	32.0	35.0	28.0	37.0
88-89	32.92971487123029	35.0	32.0	35.0	28.0	36.0
90-91	32.786575946065966	34.0	32.0	35.0	27.0	36.0
92-93	32.77573749552282	34.0	32.0	35.0	28.0	36.0
94-95	32.71212351091337	34.0	32.0	35.0	28.0	35.0
96-97	32.81954958686009	34.0	32.0	35.0	29.0	35.0
98-99	32.951152248699344	34.0	32.0	35.0	29.0	35.0
100	31.86623651193808	34.0	31.0	35.0	25.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	0.0
18	2.0
19	14.0
20	117.0
21	595.0
22	2737.0
23	8781.0
24	22721.0
25	47417.0
26	83036.0
27	129340.0
28	182060.0
29	236848.0
30	296241.0
31	360021.0
32	437066.0
33	539082.0
34	689923.0
35	906250.0
36	1300129.0
37	1961619.0
38	2132274.0
39	1063593.0
40	35597.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.5621672404792	20.49628075953307	13.642009593440216	24.29954240654752
2	15.610125242155021	21.358896930697092	39.62558828241849	23.405389544729395
3	18.692215602373103	25.662666615942136	30.29158969746492	25.35352808421984
4	13.075527834699061	17.386395085067612	37.981301071040065	31.556776009193264
5	13.261882749056486	37.825361670549576	35.533915885292686	13.378839695101243
6	31.046333924394737	37.411484530060186	18.059637788985714	13.482543756559362
7	27.16978372978911	32.204202898884034	22.75708104594103	17.86893232538582
8	25.030741325924748	36.43024402173205	20.834876149254118	17.70413850308908
9	25.62680490297317	16.274475193436537	21.18036150572701	36.91835839786329
10-11	24.7335001107761	26.51976950905106	28.569640027506203	20.17709035266664
12-13	25.082602939361394	24.032462763514875	28.88532316339743	21.9996111337263
14-15	22.16899028160128	25.58248967175777	26.288054848351738	25.960465198289217
16-17	21.947241636787783	28.607851073991537	27.13589927577729	22.30900801344339
18-19	21.951416311521694	27.940515323360604	28.662081106015957	21.445987259101745
20-21	22.649394086049842	27.127872041707196	28.594068799624328	21.62866507261863
22-23	22.39950611672727	27.00052893472298	28.416158958183104	22.18380599036665
24-25	22.188027480403306	27.1642476158419	28.53203622337489	22.115688680379897
26-27	22.151951610506824	27.32489708715179	28.429177342742634	22.09397395959875
28-29	22.113959636991343	27.36191968599509	28.462900932134875	22.06121974487869
30-31	22.353134291300165	27.292861733192925	28.29881633902375	22.055187636483158
32-33	22.13201080196325	27.45646252535463	28.252261618252465	22.15926505442965
34-35	22.301648389296957	27.449186507134236	28.285489665879204	21.9636754376896
36-37	22.306530443128665	27.34289790597461	28.227463402418852	22.12310824847787
38-39	22.114723208388952	27.55161518112037	28.254675836014375	22.078985774476294
40-41	22.405331007622976	27.392513089439046	28.136080848270375	22.066075054667607
42-43	22.259916080776325	27.466109977929047	27.958100019066855	22.315873922227773
44-45	22.313984118718093	27.615929936862592	27.820990305753323	22.24909563866599
46-47	22.369952630889358	27.53811646484241	27.629436413140134	22.462494491128098
48-49	22.282982135867897	27.63155815045568	27.628812957974013	22.45664675570241
50-51	22.289211007888586	27.758860993427003	27.537323663660967	22.414604335023448
52-53	22.47066650103912	27.641549709943426	27.409078854584422	22.478704934433036
54-55	22.46283673391192	27.56220856719352	27.453664578517827	22.521290120376737
56-57	22.37032832405084	27.731330339676557	27.452803748070924	22.445537588201677
58-59	22.35315310162778	27.774391518010905	27.453452852324105	22.41900252803721
60-61	22.44957247425624	27.74633185473061	27.465057707111846	22.339037963901305
62-63	22.47507544739386	27.736162532031127	27.4239136965511	22.36484832402391
64-65	22.379901938997328	27.705522218281075	27.411516992069494	22.5030588506521
66-67	22.452362016120407	27.744486226029853	27.259042728841386	22.544109029008357
68-69	22.367622760955587	27.721695733242353	27.407139168941967	22.50354233686009
70-71	22.358898087618524	27.500311972097574	27.490909417367792	22.649880522916106
72-73	22.45989655491295	27.566786552928036	27.394598688061812	22.578718204097207
74-75	22.24917291615178	27.706494441160203	27.394524676903192	22.649807965784817
76-77	22.361927687954324	27.62694853566108	27.367114617392087	22.64400915899251
78-79	22.35736550785698	27.558869932792945	27.323798778279656	22.759965781070417
80-81	22.353271885996804	27.59548690620999	27.35167299530015	22.699568212493055
82-83	22.45094729646958	27.567884098204214	27.47461168930322	22.506556916022987
84-85	22.50267597601127	27.57312364557326	27.365244839736086	22.55895553867938
86-87	22.316648958690397	27.551646909522976	27.535561063800664	22.596143067985963
88-89	22.400237795686973	27.50321980493633	27.493387549425197	22.6031548499515
90-91	22.459290436119357	27.575936149506674	27.46371260177743	22.50106081259654
92-93	22.48215782908325	27.475085477384457	27.534957809267418	22.507798884264872
94-95	22.26123129385152	27.492289471545007	27.64628223432077	22.600197000282705
96-97	22.312373549995463	27.62140153696096	27.70212363559825	22.36410127744532
98-99	23.403825857553077	29.625068449244363	27.878226071443557	19.092879621759
100	30.094605543679297	41.056539035472994	28.848855420847702	0.0
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1760.0
1	1628.5
2	2408.0
3	3569.5
4	4892.0
5	7849.5
6	10553.5
7	12703.0
8	14223.0
9	14296.0
10	13035.0
11	11381.0
12	11720.0
13	15175.5
14	23787.0
15	35245.0
16	44642.5
17	50456.0
18	52456.5
19	51734.0
20	49532.0
21	47474.5
22	47658.5
23	51654.0
24	60549.0
25	74496.5
26	93706.0
27	114753.0
28	135324.0
29	159621.5
30	182293.5
31	202100.5
32	226158.5
33	251492.0
34	274759.5
35	298489.5
36	324720.0
37	347106.0
38	364296.0
39	377901.0
40	391871.5
41	406134.5
42	415403.5
43	424866.0
44	435117.5
45	441735.5
46	443999.0
47	445324.0
48	447697.0
49	447612.0
50	444213.0
51	437805.5
52	425067.5
53	407286.0
54	388247.0
55	366697.5
56	340414.0
57	311337.0
58	280793.0
59	244860.0
60	207054.0
61	171155.5
62	137679.5
63	108497.0
64	83450.5
65	63919.0
66	48600.0
67	37286.5
68	28625.5
69	21100.0
70	15145.0
71	10647.0
72	7466.5
73	5127.5
74	3462.5
75	2344.5
76	1614.5
77	1106.0
78	761.5
79	525.0
80	359.0
81	244.0
82	173.0
83	117.0
84	73.5
85	55.0
86	44.5
87	34.0
88	26.0
89	18.0
90	13.5
91	9.5
92	6.5
93	5.0
94	3.0
95	1.5
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	1.2457519857286652E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0016434343504035853
20-21	0.003100151619934636
22-23	0.0049838243750128484
24-25	0.001327783549615053
26-27	0.001649762832205404
28-29	0.004233219484366989
30-31	0.008134597394640378
32-33	0.014350914087162995
34-35	2.0822429805167917E-4
36-37	8.770975361209475E-4
38-39	0.002098688491716324
40-41	0.0027089333029363893
42-43	3.1569202849566537E-4
44-45	2.5341116772879737E-5
46-47	0.003773409191008279
48-49	0.003800491360380944
50-51	9.784750224786226E-4
52-53	0.0012820897488359582
54-55	0.001017085037511334
56-57	0.0016239400589555635
58-59	6.183006503473939E-4
60-61	4.0822555479670344E-4
62-63	0.001653521079703963
64-65	4.530548051795519E-4
66-67	0.002133605938796206
68-69	0.001633650414852843
70-71	6.942640205870828E-4
72-73	6.254051471693677E-4
74-75	0.008362671963600332
76-77	0.003099562961622411
78-79	0.006445452314691343
80-81	9.024168334257773E-5
82-83	0.00518262393956864
84-85	0.002903120794927742
86-87	8.533891639488592E-4
88-89	0.0033463048066943207
90-91	8.100428977587099E-4
92-93	2.8802075592775518E-5
94-95	6.128072582368309E-4
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	324.0
20-21	995.0
22-23	2051.0
24-25	4830.0
26-27	6986.0
28-29	13453.0
30-31	29347.0
32-33	39507.0
34-35	57291.0
36-37	83430.0
38-39	95858.0
40-41	97889.0
42-43	107517.0
44-45	124844.0
46-47	124584.0
48-49	115121.0
50-51	106924.0
52-53	105355.0
54-55	113963.0
56-57	118701.0
58-59	116637.0
60-61	112752.0
62-63	110349.0
64-65	115696.0
66-67	122461.0
68-69	123535.0
70-71	121569.0
72-73	115069.0
74-75	118516.0
76-77	117694.0
78-79	123912.0
80-81	126339.0
82-83	121818.0
84-85	125471.0
86-87	132440.0
88-89	145318.0
90-91	153270.0
92-93	166288.0
94-95	191429.0
96-97	394865.0
98-99	2066828.0
100-101	4164238.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.64804130118446
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.65656801825152	75.8925287253141
2	7.057899764176597	11.94874781475281
3	1.802022585068945	4.576130466197497
4	0.6837555564899652	2.315142743427078
5	0.32076095444754876	1.357589325994173
6	0.1583278386046294	0.8041284852799162
7	0.09635347508774858	0.5709293057118272
8	0.06218500683159736	0.42110712212763934
9	0.03390123856537996	0.2582706098019231
>10	0.12523273344061747	1.6014643702625422
>50	0.002280170997228015	0.12879237621267609
>100	6.561055654263767E-4	0.08834115789645106
>500	3.7703203483574474E-5	0.018526495707219766
>1k	1.88492693767987E-5	0.01830100131411956
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	9.582707582528195E-6
2	0.0	2.874812274758458E-5	0.0	0.0	9.582707582528195E-6
3	0.0	2.874812274758458E-5	0.0	9.582707582528195E-6	9.582707582528195E-6
4	0.0	5.749624549516916E-5	0.0	3.833083033011278E-5	9.582707582528195E-6
5	0.0	6.707895307769735E-5	0.0	4.791353791264097E-5	9.582707582528195E-6
6	0.0	6.707895307769735E-5	0.0	4.791353791264097E-5	9.582707582528195E-6
7	0.0	6.707895307769735E-5	0.0	5.749624549516916E-5	9.582707582528195E-6
8	0.0	8.624436824275375E-5	0.0	5.749624549516916E-5	9.582707582528195E-6
9	0.0	2.683158123107894E-4	0.0	7.666166066022556E-5	9.582707582528195E-6
10-11	0.0	2.683158123107894E-4	0.0	1.48531967529187E-4	9.582707582528195E-6
12-13	0.0	2.683158123107894E-4	0.0	1.629060289029793E-4	9.582707582528195E-6
14-15	0.0	3.9768236467492004E-4	0.0	1.820714440680357E-4	2.874812274758458E-5
16-17	0.0	4.168477798399765E-4	0.0	1.9165415165056388E-4	2.874812274758458E-5
18-19	0.0	5.222575632477866E-4	0.0	2.1081956681562027E-4	3.833083033011278E-5
20-21	0.0	6.037105776992763E-4	0.0	2.44359043354469E-4	4.3122184121376875E-5
22-23	0.0	6.659981769857095E-4	0.0	2.683158123107894E-4	5.270489170390507E-5
24-25	0.0	7.42659837645935E-4	0.0	2.97063935058374E-4	6.707895307769735E-5
26-27	0.0	7.809906679760478E-4	0.0	3.92891010883656E-4	6.707895307769735E-5
28-29	0.0	7.90573375558576E-4	0.0	7.761993141847836E-4	6.707895307769735E-5
30-31	0.0	8.720263900100657E-4	0.0	0.001930915577879431	6.707895307769735E-5
32-33	0.0	8.816090975925938E-4	0.0	0.004197225921147349	8.624436824275375E-5
34-35	0.0	0.0010109756499567246	0.0	0.007536799513658425	8.624436824275375E-5
36-37	0.0	0.0011259681409470628	0.0	0.01306123043498593	8.624436824275375E-5
38-39	0.0	0.001183464386442232	0.0	0.020952590129197896	8.624436824275375E-5
40-41	0.0	0.0012457519857286652	0.0	0.029615357783803385	8.624436824275375E-5
42-43	0.0	0.0012888741698500422	0.0	0.037636084030379484	8.624436824275375E-5
44-45	0.0	0.0015188591518307188	0.0	0.04673965623378126	9.103572203401785E-5
46-47	0.0	0.0016865565345249621	0.0	0.05623611944806671	1.0061842961654604E-4
48-49	0.0	0.0017009305958987544	0.0	0.06586194921471628	1.2457519857286652E-4
50-51	0.0	0.0019452896392532235	0.0	0.0748265721581714	1.2457519857286652E-4
52-53	0.0	0.0019644550544182797	0.0	0.0838486913471217	1.2457519857286652E-4
54-55	0.0	0.0019884118233746	0.0	0.09263124284650878	1.2457519857286652E-4
56-57	0.0	0.002021951299913449	0.0	0.10081487512198786	1.2457519857286652E-4
58-59	0.0	0.002045908068869769	0.0	0.10885476678372902	1.2457519857286652E-4
60-61	9.582707582528195E-6	0.002093821606782411	0.0	0.11707193853574695	1.2457519857286652E-4
62-63	9.582707582528195E-6	0.002160900559860108	0.0	0.1246326948183617	1.2457519857286652E-4
64-65	9.582707582528195E-6	0.0024004682494233125	0.0	0.13208804131756863	1.2457519857286652E-4
66-67	9.582707582528195E-6	0.0024052596032145767	0.0	0.13957213593952314	1.2936655236413063E-4
68-69	9.582707582528195E-6	0.002481921263874802	0.0	0.14690290724015723	1.341579061553947E-4
70-71	9.582707582528195E-6	0.002510669386622387	0.0	0.15351976682589294	1.341579061553947E-4
72-73	9.582707582528195E-6	0.0025394175093699715	0.0	0.15920231242233215	1.9165415165056388E-4
74-75	9.582707582528195E-6	0.0025394175093699715	0.0	0.16355286166479996	1.9165415165056388E-4
76-77	9.582707582528195E-6	0.002601705108656405	0.0	0.16763988644874822	1.9165415165056388E-4
78-79	9.582707582528195E-6	0.002644827292777782	0.0	0.17024159155740465	1.9165415165056388E-4
80-81	9.582707582528195E-6	0.002644827292777782	0.0	0.17105133034812828	1.9165415165056388E-4
82-83	9.582707582528195E-6	0.00265441000036031	0.0	0.17178919883198293	2.0123685923309208E-4
84-85	9.582707582528195E-6	0.0026735754155253664	0.0	0.1719952270450073	2.0123685923309208E-4
86-87	9.582707582528195E-6	0.002731071661020535	0.0	0.17214375901253648	2.0602821302435616E-4
88	9.582707582528195E-6	0.0028460641520108737	0.0	0.17217250713528406	2.1081956681562027E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11805	0.0	45.452576	1
GTATCAA	20255	0.0	32.511227	1
TCAACGC	23605	0.0	27.087896	4
ATCAACG	23955	0.0	26.72606	3
CAACGCA	24080	0.0	26.519802	5
TATCAAC	25015	0.0	25.804804	2
AACGCAG	24915	0.0	25.712595	6
ACGCAGA	29675	0.0	21.560785	7
GTACATG	30195	0.0	21.512571	1
CGCAGAG	30635	0.0	20.911678	8
TACATGG	30545	0.0	20.760366	2
ACATGGG	30305	0.0	20.32118	3
GTGGTAT	6650	0.0	18.154545	1
CATGGGG	22200	0.0	18.127285	4
GAGTACT	20375	0.0	17.756422	12-13
GCAGAGT	35770	0.0	17.523304	9
TGGTATC	6630	0.0	17.35096	2
CAGAGTA	34905	0.0	16.414513	10-11
GAGTACA	21785	0.0	16.06557	1
AGAGTAC	31595	0.0	16.017752	10-11
>>END_MODULE