FastQCFastQC Report
Fri 27 May 2016
SRR2138715_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138715_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7320342
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA83400.049.760871
GTATCAA144750.033.1529241
TCAACGC180200.026.08744
ATCAACG184450.025.442063
TATCAAC188100.025.1219222
CAACGCA187300.025.1202895
AACGCAG192000.024.4841126
GTACATG241500.021.5946831
TACATGG238400.021.3619772
GTGGTAT43050.020.5693071
ACGCAGA230000.020.5098787
ACATGGG245200.020.2702883
CGCAGAG236200.019.9369648
TGGTATC41900.019.478212
CATGGGG189600.018.122034
GAGTACT143900.017.695512-13
GCAGAGT279650.016.6787879
CAGAGTA263000.016.45462610-11
AGAGTAC242800.016.36979910-11
GTACTTT161250.015.89491814-15
Produced by FastQC (version 0.11.5)