##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138715_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7320342
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.75612888031734	31.0	31.0	34.0	30.0	34.0
2	32.02389779056771	33.0	31.0	34.0	30.0	34.0
3	31.930427294243902	31.0	31.0	34.0	30.0	34.0
4	35.788188174814785	37.0	35.0	37.0	35.0	37.0
5	35.56654019716565	37.0	35.0	37.0	33.0	37.0
6	35.65372983939822	37.0	35.0	37.0	33.0	37.0
7	35.57018620168293	37.0	35.0	37.0	33.0	37.0
8	35.55524154472564	37.0	35.0	37.0	33.0	37.0
9	37.275820310034696	39.0	37.0	39.0	34.0	39.0
10-11	37.147697266056696	39.0	37.0	39.0	33.0	39.0
12-13	37.10653716725257	39.0	37.0	39.0	33.0	39.0
14-15	38.291586035187976	40.0	38.0	41.0	33.0	41.0
16-17	38.24449861768753	40.0	38.0	41.0	33.0	41.0
18-19	38.2427067615147	40.0	38.0	41.0	33.0	41.0
20-21	38.23952155335194	40.0	38.0	41.0	33.0	41.0
22-23	38.17141657466017	40.0	37.5	41.0	33.0	41.0
24-25	38.09095825752246	40.0	37.0	41.0	33.0	41.0
26-27	37.923340995462596	40.0	37.0	41.0	33.0	41.0
28-29	37.770590726234055	40.0	37.0	41.0	32.0	41.0
30-31	37.613472809868014	39.5	37.0	41.0	32.0	41.0
32-33	37.44782875503228	39.0	36.0	41.0	32.0	41.0
34-35	37.31601998375351	39.0	36.0	41.0	31.0	41.0
36-37	37.4060246055136	39.0	36.0	41.0	31.0	41.0
38-39	37.372891916050335	39.0	36.0	41.0	31.0	41.0
40-41	37.33513427551459	39.0	36.0	41.0	31.0	41.0
42-43	37.41130691719197	39.0	36.0	41.0	31.0	41.0
44-45	37.44739957986334	40.0	36.0	41.0	31.5	41.0
46-47	37.32684042092369	39.0	36.0	41.0	31.0	41.0
48-49	37.26020368905603	39.0	36.0	41.0	31.0	41.0
50-51	37.15984219656531	39.0	36.0	41.0	31.0	41.0
52-53	37.00296223076827	39.0	35.0	41.0	31.0	41.0
54-55	36.82353340549737	39.0	35.0	40.0	31.0	41.0
56-57	36.67979114188823	39.0	35.0	40.0	30.0	41.0
58-59	36.414913877212335	38.0	35.0	40.0	30.0	41.0
60-61	36.249274113457346	38.0	34.5	40.0	30.0	41.0
62-63	35.98186777214205	37.5	34.0	40.0	29.0	41.0
64-65	35.66896382152464	37.0	34.0	40.0	29.0	41.0
66-67	35.337694721386725	36.5	34.0	39.0	29.0	41.0
68-69	35.10270144207509	36.0	34.0	39.0	29.0	41.0
70-71	34.77677814114344	35.5	33.0	39.0	28.0	40.0
72-73	34.48561425808521	35.0	33.0	38.0	28.0	40.0
74-75	34.204362293405524	35.0	33.0	37.0	28.0	39.5
76-77	32.85691838236894	34.0	31.5	36.0	26.5	39.0
78-79	33.61123198804419	35.0	32.5	36.0	28.0	39.0
80-81	33.65208288230764	35.0	33.0	36.0	29.0	38.5
82-83	33.4319900462052	35.0	33.0	36.0	28.5	37.0
84-85	33.234172273581635	35.0	33.0	35.0	28.0	37.0
86-87	33.068508346098525	35.0	32.0	35.0	28.0	36.5
88-89	32.95734025497397	35.0	32.0	35.0	28.0	36.0
90-91	32.82480965123389	34.0	32.0	35.0	28.0	36.0
92-93	32.82391862814946	34.0	32.0	35.0	28.5	36.0
94-95	32.76662781631964	34.0	32.0	35.0	29.0	35.0
96-97	32.868244829357565	34.0	32.0	35.0	29.0	35.0
98-99	32.99615869298091	34.0	32.0	35.0	29.0	35.0
100	31.940234506708673	34.0	31.0	35.0	26.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	2.0
17	1.0
18	1.0
19	12.0
20	71.0
21	379.0
22	1747.0
23	5982.0
24	15319.0
25	31582.0
26	56183.0
27	87476.0
28	123222.0
29	162778.0
30	203091.0
31	246861.0
32	299940.0
33	370676.0
34	477773.0
35	637031.0
36	927860.0
37	1413232.0
38	1495028.0
39	738967.0
40	25128.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.41508115331224	19.749022654952462	13.555678136349366	24.280218055385937
2	15.873001561948882	20.89618217290941	39.357286859001945	23.873529406139767
3	19.007328090942423	25.049205472326086	29.955887198435477	25.98757923829601
4	13.382366561562286	17.06047340411145	37.107542243244914	32.449617791081344
5	13.773331901706232	37.44263587684838	34.93564918141803	13.848383040027365
6	31.86465878233558	36.44265527484918	17.777366139450862	13.91531980336438
7	27.937588161864568	31.9873852888294	22.002400980719205	18.072625568586822
8	25.73497249172238	35.860633287351874	20.34257415842047	18.061820062505276
9	26.061678539062793	16.13339923189381	20.588723860169374	37.21619836887402
10-11	25.270287918242072	26.184186476533473	27.93211027572209	20.613415329502367
12-13	25.65066632132761	23.614033333415296	28.33073236195795	22.40456798329914
14-15	22.729423024224822	25.03776462902963	25.801205736016158	26.431606610729393
16-17	22.504966298022687	28.170821800402223	26.55847909838092	22.765732803194165
18-19	22.5550961811743	27.36791502825427	28.168123054746363	21.90886573582507
20-21	23.320792881861596	26.51407235109147	28.089878123369573	22.075256643677356
22-23	23.072741042892922	26.411105674618685	27.934486455732888	22.5816668267555
24-25	22.81124038611538	26.52354921281915	28.042084654594397	22.623125746471075
26-27	22.735119709058413	26.67551767349547	28.00498028074887	22.58438233669725
28-29	22.7417709079221	26.736925890860956	27.997433542137408	22.523869659079544
30-31	22.967176764785442	26.65677599520741	27.81228760237664	22.563759637630508
32-33	22.74053836908795	26.85058702902191	27.789614869697125	22.619259732193015
34-35	22.85249569290523	26.847379139646478	27.792321264451374	22.50780390299692
36-37	22.877568861228752	26.723966476751016	27.741636039346158	22.656828622674073
38-39	22.6827551765687	26.91648442331133	27.845538826146317	22.555221573973654
40-41	22.99440977033167	26.788711069304444	27.64409530225798	22.572783858105904
42-43	22.792949816310713	26.865812104563975	27.529081255550803	22.81215682357451
44-45	22.882122304136747	26.97999519763062	27.354395469135333	22.783487029097298
46-47	22.984605041329765	26.91898256547119	27.156562557828273	22.939849835370772
48-49	22.881682564217368	27.05123561295743	27.10871320190819	22.958368620917007
50-51	22.86437315370891	27.19732937862941	27.032445379091147	22.905852088570537
52-53	23.064285734259173	27.07867616667575	26.87421491496992	22.98282318409516
54-55	23.04171640802181	26.92580619968201	26.99990050455348	23.032576887742696
56-57	22.94412201090652	27.08598221375577	26.986851755566693	22.983044019771018
58-59	22.934044063784015	27.167839102456853	26.958487877414978	22.939628956344155
60-61	22.999244493630698	27.05169627279823	27.018616102040788	22.930443131530282
62-63	23.060355503707864	27.202709532539803	26.8724802363013	22.86445472745104
64-65	22.967070614871236	27.166021370958397	26.905315673350188	22.96159234082017
66-67	23.047986340943176	27.134435604744294	26.768504471160036	23.04907358315249
68-69	22.945289295602457	27.119421463924944	26.922933636848263	23.01235560362434
70-71	22.93202488195037	26.874350082029576	27.001111887691803	23.19251314832826
72-73	23.02288929059859	26.96351369801162	26.870822033636898	23.142774977752882
74-75	22.808340523519924	27.123680850500335	26.902678443239807	23.16530018273993
76-77	23.014977286668202	27.001287867962116	26.85231654951425	23.13141829585543
78-79	22.91267569756564	27.00784724906786	26.822953898645007	23.25652315472149
80-81	22.98126907912675	27.01344258253839	26.8010436018713	23.20424473646356
82-83	22.960113913696787	26.96786614733317	26.979150505380016	23.092869433590025
84-85	23.069751031503248	26.933845841502308	26.876794263659914	23.119608863334527
86-87	22.891160010298346	26.977266489183055	27.018289696501878	23.113283804016717
88-89	22.92469140093108	26.923514626494345	26.971868685290428	23.17992528728415
90-91	22.950943430711675	26.92156324791198	27.03154424162284	23.095949079753506
92-93	22.997881312936574	26.84081232572581	27.018495894536464	23.142810466801148
94-95	22.879503275563202	26.905106401738095	27.07388565972072	23.141504662977987
96-97	22.837740864044072	27.04487414957993	27.16784480907461	22.94954017730139
98-99	24.011081871954605	29.013311133895964	27.3140260138996	19.66158098024983
100	31.05285717600903	40.66549588497308	28.28164693901788	0.0
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1735.0
1	1487.0
2	1888.0
3	2662.5
4	3479.5
5	5371.5
6	7138.0
7	8838.0
8	10305.5
9	10577.0
10	9542.0
11	8214.5
12	8533.0
13	11162.5
14	17509.0
15	26059.5
16	32959.0
17	36782.0
18	37613.0
19	36303.5
20	34001.5
21	31663.0
22	30778.5
23	32826.0
24	38235.5
25	46899.0
26	58830.5
27	71903.0
28	84567.5
29	99172.0
30	112992.0
31	125332.0
32	139037.0
33	153396.5
34	166816.5
35	180083.5
36	195503.0
37	209728.0
38	222286.5
39	233139.5
40	243937.5
41	255082.5
42	264312.0
43	274036.0
44	285018.5
45	294759.0
46	302609.0
47	309818.5
48	316042.0
49	319902.0
50	321740.5
51	321867.5
52	316618.5
53	309152.5
54	300468.5
55	287147.5
56	270015.5
57	250978.0
58	229855.0
59	203441.0
60	174740.5
61	146431.0
62	119434.5
63	94438.0
64	72450.5
65	56500.5
66	43979.5
67	33225.5
68	24973.5
69	18532.0
70	13237.0
71	9158.0
72	6200.0
73	4096.0
74	2754.5
75	1886.5
76	1310.0
77	896.0
78	601.5
79	405.0
80	281.0
81	181.0
82	118.0
83	74.0
84	49.5
85	35.0
86	26.0
87	21.5
88	16.0
89	12.0
90	8.5
91	6.0
92	5.0
93	3.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	2.458901510339271E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0016460979555326786
20-21	0.0032172067418718914
22-23	0.0052259556343008775
24-25	0.0011821085345093013
26-27	0.0018320578541995039
28-29	0.004426382856393431
30-31	0.008470343074250713
32-33	0.0145570912210018
34-35	1.794116443539535E-4
36-37	8.885621571790095E-4
38-39	0.0021277312037170345
40-41	0.0027258335436556366
42-43	3.707352171292788E-4
44-45	1.4417198102610226E-5
46-47	0.0038541572304902085
48-49	0.003887364859566727
50-51	8.896808460610652E-4
52-53	0.0011865171745715918
54-55	0.0010316241715198214
56-57	0.0014309357321900906
58-59	7.441246075036429E-4
60-61	4.044789941765335E-4
62-63	0.0018305147286988503
64-65	4.958057273852035E-4
66-67	0.002050892105119669
68-69	0.0018292754741018446
70-71	4.914209343115096E-4
72-73	9.193471363711426E-4
74-75	0.00819718614381105
76-77	0.0031725103305419956
78-79	0.006547167408380015
80-81	6.376975837638551E-5
82-83	0.005007819511149803
84-85	0.0029902520603824045
86-87	7.456971125556178E-4
88-89	0.0035726405434930533
90-91	9.436502716868463E-4
92-93	4.058785830453963E-5
94-95	6.758194805069228E-4
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	207.0
20-21	629.0
22-23	1359.0
24-25	2974.0
26-27	4734.0
28-29	9723.0
30-31	19899.0
32-33	26494.0
34-35	38482.0
36-37	55858.0
38-39	64077.0
40-41	65496.0
42-43	73215.0
44-45	85710.0
46-47	85719.0
48-49	79614.0
50-51	72785.0
52-53	71136.0
54-55	77639.0
56-57	81046.0
58-59	79653.0
60-61	77354.0
62-63	75476.0
64-65	79656.0
66-67	84155.0
68-69	84844.0
70-71	83227.0
72-73	79281.0
74-75	82013.0
76-77	81420.0
78-79	86189.0
80-81	87414.0
82-83	84307.0
84-85	86221.0
86-87	91492.0
88-89	100732.0
90-91	105190.0
92-93	114718.0
94-95	132338.0
96-97	283402.0
98-99	1494228.0
100-101	2930236.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.23071188674498
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.26122633412022	79.60781740782666
2	6.284686786095059	10.964354046725827
3	1.3899134660826664	3.637294233220925
4	0.49490016090653455	1.7268197339496663
5	0.21975001870588518	0.9584475284419944
6	0.11690792304015586	0.6118776811196156
7	0.06681338230371865	0.4079725231340239
8	0.04438135375407478	0.30971336659723236
9	0.021992359811470227	0.17265682821815898
>10	0.09648202060681586	1.317270841955822
>50	0.0021039645764407834	0.1243450040089263
>100	7.700544474549693E-4	0.10766360932098284
>500	4.811703424521526E-5	0.03242401230099682
>1k	2.405851526918141E-5	0.021343183179045884
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.7321127892658566E-5	0.0	0.0	0.0
3	0.0	2.7321127892658566E-5	0.0	0.0	0.0
4	0.0	6.830281973164642E-5	0.0	1.3660563946329283E-5	0.0
5	0.0	8.19633836779757E-5	0.0	1.3660563946329283E-5	0.0
6	2.7321127892658566E-5	8.19633836779757E-5	0.0	1.3660563946329283E-5	0.0
7	2.7321127892658566E-5	8.19633836779757E-5	0.0	1.3660563946329283E-5	0.0
8	2.7321127892658566E-5	9.562394762430498E-5	0.0	1.3660563946329283E-5	1.3660563946329283E-5
9	2.7321127892658566E-5	2.86871842872915E-4	0.0	4.098169183898785E-5	1.3660563946329283E-5
10-11	4.098169183898785E-5	2.86871842872915E-4	0.0	4.098169183898785E-5	1.3660563946329283E-5
12-13	5.464225578531713E-5	3.073626887924089E-4	0.0	4.098169183898785E-5	1.3660563946329283E-5
14-15	5.464225578531713E-5	4.507986102288664E-4	0.0	4.098169183898785E-5	5.464225578531713E-5
16-17	1.0928451157063426E-4	4.507986102288664E-4	0.0	4.098169183898785E-5	8.879366565114034E-5
18-19	1.0928451157063426E-4	4.781197381215249E-4	0.0	4.781197381215249E-5	1.1611479354379892E-4
20-21	1.0928451157063426E-4	5.054408660141834E-4	0.0	5.464225578531713E-5	1.434359214364575E-4
22-23	1.0928451157063426E-4	6.147253775848178E-4	0.0	9.562394762430498E-5	1.5709648538278677E-4
24-25	1.0928451157063426E-4	7.581612990212752E-4	0.0	1.3660563946329285E-4	1.7075704932911605E-4
26-27	1.0928451157063426E-4	8.606155286187448E-4	0.0	1.9124789524860997E-4	1.7758733130228069E-4
28-29	1.0928451157063426E-4	8.606155286187448E-4	0.0	5.054408660141836E-4	1.9124789524860997E-4
30-31	1.0928451157063426E-4	9.289183483503913E-4	0.0	0.0013114141388476113	2.1173874116810388E-4
32-33	1.0928451157063426E-4	9.699000401893792E-4	0.0	0.0033400078848775097	2.458901510339271E-4
34-35	1.3660563946329285E-4	0.0011543176534648245	0.0	0.0063931439268821045	2.732112789265857E-4
36-37	1.3660563946329285E-4	0.0013455655487134345	0.0	0.011283625819667988	2.732112789265857E-4
38-39	1.3660563946329285E-4	0.0013797169585792577	0.0	0.01874229373436378	2.732112789265857E-4
40-41	1.3660563946329285E-4	0.001543643725935209	0.0	0.0260780165735426	2.8004156089975034E-4
42-43	1.3660563946329285E-4	0.0015982859817205261	0.0	0.03332494574707029	2.86871842872915E-4
44-45	1.434359214364575E-4	0.001789533876969136	0.0	0.041241242553968105	2.86871842872915E-4
46-47	1.5709648538278677E-4	0.0019466303623519227	0.0	0.04982007671226289	2.937021248460796E-4
48-49	1.639267673559514E-4	0.001960290926298252	0.0	0.05837158974266503	3.0053240681924426E-4
50-51	1.7075704932911605E-4	0.002213011359305344	0.0	0.06717582320607425	3.0053240681924426E-4
52-53	1.7758733130228069E-4	0.0022266719232516734	0.0	0.0753585010099255	3.073626887924089E-4
54-55	1.7758733130228069E-4	0.0022813141790369905	0.0	0.08415590419136154	3.1419297076557353E-4
56-57	1.7758733130228069E-4	0.0023359564348223076	0.0	0.09258447214624671	3.210232527387382E-4
58-59	1.7758733130228069E-4	0.002349616998768637	0.0	0.10076714995009796	3.278535347119028E-4
60-61	1.8441761327544533E-4	0.0024110895365271187	0.0	0.10926402072471478	3.278535347119028E-4
62-63	2.458901510339271E-4	0.002479392356258765	0.0	0.11722129922345159	3.415140986582321E-4
64-65	2.458901510339271E-4	0.0029097001205681374	0.0	0.12487804531536914	3.415140986582321E-4
66-67	2.5955071498025636E-4	0.002937021248460796	0.0	0.1326099245089915	3.5517466260456137E-4
68-69	2.5955071498025636E-4	0.0030189846321387716	0.0	0.14038961567642605	3.8249579049721993E-4
70-71	2.86871842872915E-4	0.0030599663239777595	0.0	0.14708329201012738	4.1664720036304313E-4
72-73	3.8249579049721993E-4	0.0030736268879240887	0.0	0.15230162743762518	4.507986102288664E-4
74-75	3.8249579049721993E-4	0.003107778297789912	0.0	0.15665934733650422	4.6445917417519566E-4
76-77	3.8932607247038457E-4	0.0032102325273873815	0.0	0.16057992918910072	4.6445917417519566E-4
78-79	3.961563544435492E-4	0.003292195911065357	0.0	0.1634623081817762	4.781197381215249E-4
80-81	3.961563544435492E-4	0.0033058564750116865	0.0	0.16450051104169722	4.917803020678542E-4
82-83	3.961563544435492E-4	0.0033195170389580158	0.0	0.16514255754717472	4.986105840410188E-4
84-85	3.961563544435492E-4	0.0033263473209311804	0.0	0.16538161741623547	5.054408660141834E-4
86-87	4.098169183898785E-4	0.0033604987307970036	0.0	0.1654909019278061	5.054408660141834E-4
88	4.098169183898785E-4	0.00342880155052865	0.0	0.16549773220977926	5.054408660141834E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8340	0.0	49.76087	1
GTATCAA	14475	0.0	33.152924	1
TCAACGC	18020	0.0	26.0874	4
ATCAACG	18445	0.0	25.44206	3
TATCAAC	18810	0.0	25.121922	2
CAACGCA	18730	0.0	25.120289	5
AACGCAG	19200	0.0	24.484112	6
GTACATG	24150	0.0	21.594683	1
TACATGG	23840	0.0	21.361977	2
GTGGTAT	4305	0.0	20.569307	1
ACGCAGA	23000	0.0	20.509878	7
ACATGGG	24520	0.0	20.270288	3
CGCAGAG	23620	0.0	19.936964	8
TGGTATC	4190	0.0	19.47821	2
CATGGGG	18960	0.0	18.12203	4
GAGTACT	14390	0.0	17.6955	12-13
GCAGAGT	27965	0.0	16.678787	9
CAGAGTA	26300	0.0	16.454626	10-11
AGAGTAC	24280	0.0	16.369799	10-11
GTACTTT	16125	0.0	15.894918	14-15
>>END_MODULE